Status
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Gene information
General information
TC012050 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7068480..7074837
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012050
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05159

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule lateral decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment: just on one side
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012050 RA
        MWAQRTGPT IYVGESPPL NQLGLAILS PEDSSGSSR 
        ASSKDSAWS EPTWGHSKC IEHGHHHVT WPLNKDEQK 
        HHDLFGCSF DPKKCSEFD CDDPARSPL NQGMVHPEC 
        ASHGHHHAT FTLSPEEQA KHEAMPCGF DPKDCPDFE 
        CSPMPEIPT SIIMRPGYH HRTYPYTED DLQRYEDAK 
        YKSIPDYWE ESRFFWPSV TDPSGGGRA LGVTFNIGP 
        EVATNINVG TPEKPSFNL PRSKISTPV KGIAGTSPP 
        SSLASGQSG SRVSQGSPP SSFASGPCG PHLSRASTP 
        SSFASGASS HLSRGSPPS SMASGYSGS RISGTSPPS 
        SMASGYSGS RISGTSPPS SMASGASGG SSSSYYTGR 
        SDSDMSSPY ATAGSSLSS SSELSTSSS LEGRPAPCG 
        SLYQADAVF EVTPSESVG EILKAIDSQ HAMESEQIL 
        GNLKRYQDA RRKFMGS
      CDS sequence
      • >TC012050 RA
        atgtgggcc caacgcaca ggtcccacc atttacgtg 
        ggtgaatcc cccccctta aaccaactc ggcttggct 
        attctaagt ccagaggac agctccggc agcagccgc 
        gcctcgtct aaagacagt gcctggtcc gaacctaca 
        tggggccac tccaaatgc atagaacat gggcatcac 
        cacgtcaca tggcccctc aataaagac gagcaaaaa 
        catcacgac ttgttcggg tgctcgttc gatccgaaa 
        aaatgctca gagtttgac tgtgacgat ccagctagg 
        tcaccactg aaccaaggc atggtgcat ccagaatgc 
        gcatcacac ggccaccac cacgcaacg ttcactcta 
        tcgccagaa gaacaagcg aaacacgag gcaatgccg 
        tgtggcttc gacccgaag gactgtcca gactttgag 
        tgctcccca atgccggag ataccaaca tccataatc 
        atgcgacct gggtatcac cacaggacc tacccctac 
        actgaagat gacctacaa agatacgag gatgctaaa 
        tataaatca attccggat tattgggaa gaatcaagg 
        tttttctgg ccttcggtt accgatccg tcagggggt 
        ggacgcgct ctaggtgtc accttcaac attggccca 
        gaggtggca acgaacatc aatgtggga acacccgaa 
        aaacctagt ttcaattta ccacgaagc aaaatatca 
        actcctgtc aagggaatt gccgggact tcaccgcca 
        agtagcttg gcttcgggg caatcaggt tcccgcgtt 
        tcccaaggt tctcctcca agcagtttc gcgtcgggt 
        ccgtgtggc ccccacctt tcgcgtgcc tcaacaccg 
        tctagtttc gcatcggga gcttcatct cacctttct 
        cgaggttct cctccaagt agtatggct tcgggctat 
        tctggttct cggatttct ggcacttct cctcccagc 
        agtatggct tcgggctat tctggttct cggatttct 
        ggcacttct cctcccagc agtatggct tcgggggca 
        tccggtgga agttcctct agttattac acgggacgt 
        tccgattct gacatgagt tcgccgtac gctacagcg 
        ggctcatcc ttatcaagt tcctcggaa ctgtctact 
        tcaagctct cttgaagga cgtcctgca ccctgcgga 
        tcactatac caagccgat gcggtcttt gaagtaacg 
        ccttcagag tccgtcgga gaaattctc aaagccata 
        gatagtcaa catgcgatg gaaagtgag caaatttta 
        ggaaacttg aaacggtat caggatgcc agaaggaag 
        tttatgggg agctaa
      mRNA sequence
      • >TC012050 RA
        gtctaattc aaggaagat tgtttaact agtaaagaa 
        tggtgatga ttttctaca gccttctgt cataatcaa 
        attaatcaa acttttcgt ccaaagtca caaattcat 
        tcatcaaaa tttctgtaa aacctccat cccagtgaa 
        acctactcg catttctat aacctgtgg ttgctgatt 
        tgagttaaa atgtgggcc caacgcaca ggtcccacc 
        atttacgtg ggtgaatcc cccccctta aaccaactc 
        ggcttggct attctaagt ccagaggac agctccggc 
        agcagccgc gcctcgtct aaagacagt gcctggtcc 
        gaacctaca tggggccac tccaaatgc atagaacat 
        gggcatcac cacgtcaca tggcccctc aataaagac 
        gagcaaaaa catcacgac ttgttcggg tgctcgttc 
        gatccgaaa aaatgctca gagtttgac tgtgacgat 
        ccagctagg tcaccactg aaccaaggc atggtgcat 
        ccagaatgc gcatcacac ggccaccac cacgcaacg 
        ttcactcta tcgccagaa gaacaagcg aaacacgag 
        gcaatgccg tgtggcttc gacccgaag gactgtcca 
        gactttgag tgctcccca atgccggag ataccaaca 
        tccataatc atgcgacct gggtatcac cacaggacc 
        tacccctac actgaagat gacctacaa agatacgag 
        gatgctaaa tataaatca attccggat tattgggaa 
        gaatcaagg tttttctgg ccttcggtt accgatccg 
        tcagggggt ggacgcgct ctaggtgtc accttcaac 
        attggccca gaggtggca acgaacatc aatgtggga 
        acacccgaa aaacctagt ttcaattta ccacgaagc 
        aaaatatca actcctgtc aagggaatt gccgggact 
        tcaccgcca agtagcttg gcttcgggg caatcaggt 
        tcccgcgtt tcccaaggt tctcctcca agcagtttc 
        gcgtcgggt ccgtgtggc ccccacctt tcgcgtgcc 
        tcaacaccg tctagtttc gcatcggga gcttcatct 
        cacctttct cgaggttct cctccaagt agtatggct 
        tcgggctat tctggttct cggatttct ggcacttct 
        cctcccagc agtatggct tcgggctat tctggttct 
        cggatttct ggcacttct cctcccagc agtatggct 
        tcgggggca tccggtgga agttcctct agttattac 
        acgggacgt tccgattct gacatgagt tcgccgtac 
        gctacagcg ggctcatcc ttatcaagt tcctcggaa 
        ctgtctact tcaagctct cttgaagga cgtcctgca 
        ccctgcgga tcactatac caagccgat gcggtcttt 
        gaagtaacg ccttcagag tccgtcgga gaaattctc 
        aaagccata gatagtcaa catgcgatg gaaagtgag 
        caaatttta ggaaacttg aaacggtat caggatgcc 
        agaaggaag tttatgggg agctaaatc acaccctcg 
        gcggagaat tatttcgta ccggcatta aaacacgcg 
        acctatact attaataat tgaaacgta aaagatagc 
        atctcacaa ataaaactt tagtactac cttaatttt 
        cataaagac gttttttta gaaatttag aactatttc 
        tttatagta ccctgctgt cagtgtcaa tttttcgtc 
        agagctcat caatccatt catcaaaat ttcagtaaa 
        aattccatt caagggaaa gtactttac atttttggc 
        aataggctg ctaatttga acaaattcc ctgtcttgc 
        gcaatatgt tcggcggcg gtggcgtgg gtggagctc 
        cggtgaacc aactcggct tggctatcc tcgaggcat 
        tggagggtt ccgacccat ttggtggcg gtttcgaca 
        gctatgaat cgtctggct cattccccc gtgccttca 
        gccttcctg aaaagggtc ccttagata acgtaatac 
        ctgggcgcc ggaacatca cgctccccc tgaatgcag 
        cacagcaaa agcagcaag atctgttcg ggtgcacgt 
        tcgatccaa gggcatgtc cagaatacg attttaaaa 
        ctccaccaa acataaata aaaccgcga gtcaagtca 
        ctatcaaca ttagcccag acgtggtaa caaatgtca 
        atgtgggaa ctcccagta tggacctct cttctgaag 
        tacccagtt ttgacttat cacgaaaca aaatgtcaa 
        ctccggtca aaagaccct catccagag tttc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY