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Gene information
General information
TC012042 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7204345..7229548
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012042
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05156

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012042 RA
      MKLFRLIVH QKNFSAEDL IINPKDYPG ANLGDIVEI 
      YHPPPDDDN PRGKKKSEP EEEYPRLLL QIKTFPDDT 
      TQQNALQKD TISVEQSIA QAFGLQAYK NVIIRIVDP 
      PDIALDSIE LTFKDQYMG RSEMWRLKN SLINTCVYI 
      KKNIEFCGG AVRCQVYEM WSMGDRVAC GVITEDTKV 
      VFRSSTSMV YLFLQMSSE MWDFDIYGD LYFEKAVNG 
      FLADLFMKW KKNGSNHEV TIVLFSRTF YEASNLEAF 
      PLHMRECLQ KDYKGRYYE DFYRVAVQN ERYDDWSGI 
      LVQLRRIFS EYLKIVLEY HKRPGLDIP AATNSTAAQ 
      GNFLEVLNM SLNVFEKHY LDRSFDRTG QLSVVVTPG 
      VGVFEVDRE LTNITKQRI IDNGVGSDL VCVGEQPLH 
      AVPLLKFHN KDTHMNVPD DYSMPHWIN LSFYSTNKK 
      IAYSNFIPR IKLPPPLPT EEVKKDSPK KLVPQDDYI 
      HNSFFDYDA YDAQIFQLP PVHTVHETS TIHHKVIPR 
      YKRSSGSLL DSHELIKGQ RRKLSDPDV PHRIDSNAR 
      SEPIAIPPA ATSPLVSPH ADEKELSPP VRMVVGSEG 
      SPPLESRAL INPFDPSHV TIKLTSNRR RWTHIFPKG 
      PTGVLIQQH HYQAIPAQP EPINSECEG NNPKLDRMI 
      SSGSAQLAK SFCATAALH EYCKKRPNS QTNHSVASA 
      PKNPSKSLT LLWGATGEQ EWTPALTTG VDWKSLTIP 
      ACLPITTDY FPDKRALQI DYVVSLYSL LPDSVNADF 
      AQQRPTYKR PLTTVEVFK ELISQRLAQ GFQLILMAN 
      NADKIPSIN ALNPKMRGP VTRISVTEN DESYYLSIG 
      RLFHKISLS GNTISVTRY RPRHPYPGF NYQYRYRFH 
      APHHDTYEE SYATFTTEK LEHFNWNYL DHYVCTGGD 
      TEFALVDAL KYWRFRVYL LPLNNSANK KIIDGAEHC 
      DIYTAATPS DHQQMVDGL LKFIETAVN KIKRTLPSK 
      KSRNVNCSS PFRERLGSN RLSDRPRPR SGSKVMERN 
      RTSQSSESQ SSSESQSHD AFPDENAVQ PGPCLKNTA 
      TSLEILEAM KNPLNGLMF ISAQQNLPS STFIASDAV 
      NWLLLRMEG VSTLDKAVQ IMQNLHLEG FIRHASGDP 
      NKQFLFGFF LFYIIEKDD KDAFQPSGD LQNFENEWL 
      EVEVKSALN WGLSDVTNG VEVPNMYCD RKYGVPHFL 
      CEDINVEYA ENESTIYKR MRLETDVAG KSDRVEWGH 
      VRYQTTFMP DQAYELIVE WLTSSGSIV AELLSSWHR 
      KAQTCGLQM IPIPHDPLA LPYSNKSDP LRGPIFVPL 
      NIKCLEERD TIFKEFKDG SSLERLFLL QETIIQRFG 
      FINCMLEPP INPEATSRE HQYVHCTGT VFILISRPA 
      LKPRSRVPS VGSKPRSTG RFSVHADVV PSPHEAYIT 
      RHVSGKNKD DYDNSRKMG FLWSWNHMV SRRWRTNQG 
      VDNNFQTKL LKDFREFCA NVDDRLRNF WEESWVTKE 
      NASTNFSLD NYMRK
    CDS sequence
    • >TC012042 RA
      atgaaattg tttcgtctt atcgtccac cagaagaat 
      ttcagtgct gaggattta atcatcaac cctaaagac 
      taccccggt gctaatttg ggcgatatt gtggagatt 
      taccacccg ccacccgac gatgacaac ccacgtggt 
      aagaagaag agcgagcct gaggaagaa tacccaagg 
      ttgttgctc caaattaaa acgtttccg gacgataca 
      acccagcaa aatgcctta caaaaagac acaatcagc 
      gtggagcag agcatagct caggctttc ggtctgcag 
      gcgtacaag aacgtgata atccgtatt gtggacccg 
      cctgacatt gcgcttgac tcaattgaa cttaccttt 
      aaagaccag tacatgggg cgctctgag atgtggcgc 
      ttgaaaaac agtcttatt aacacttgt gtgtacatc 
      aaaaagaac attgagttc tgtgggggc gctgtgcgt 
      tgccaggtc tacgagatg tggtcaatg ggcgacaga 
      gtcgcttgt ggggttatc acagaagac acgaaagtc 
      gttttccgc tcctccaca tccatggtt tatctcttc 
      ctgcaaatg tcgtcggaa atgtgggac tttgatatt 
      tacggcgac ttgtacttc gaaaaagcc gtgaacggg 
      tttttggcc gatttgttc atgaagtgg aagaagaac 
      gggagcaac cacgaagtg acgattgtt ttgttttcc 
      cgcacgttt tacgaagcg agtaacttg gaggcgttc 
      cctctccac atgcgagag tgtctccag aaggattac 
      aaaggccga tactacgag gatttttac agggttgcg 
      gtccagaac gagcgctat gacgactgg agcgggatt 
      ttggtccag ttaaggcga attttttcc gaatatttg 
      aaaattgtc ctcgagtat cacaaaagg ccagggttg 
      gatattcca gctgcgacg aacagtaca gcagctcag 
      gggaatttt ctagaggtg ttaaacatg tccctgaat 
      gtttttgag aagcattat ttggatcga agttttgat 
      aggactggg caactcagt gttgtggtt acaccgggt 
      gttggtgtt tttgaagtt gatcgagag ttgaccaat 
      attacgaaa cagagaatt atcgataat ggggttggg 
      agtgatttg gtgtgtgtt ggggaacag ccactgcac 
      gcagttccg cttctaaag tttcataat aaagacact 
      catatgaac gtccctgac gactactca atgccccac 
      tggatcaac ttatcgttt tactcaact aacaaaaaa 
      atcgcatat tcgaatttc atcccacgt attaagcta 
      ccacctcct ttacccacc gaagaggtg aaaaaggat 
      tccccgaaa aaactcgta cctcaagac gattacatt 
      cacaattcg tttttcgat tatgacgct tacgatgcg 
      cagattttt cagttaccg ccagtgcat acagtgcac 
      gagaccagt acaatacac cacaaagta atcccgcgt 
      tacaaacgc agctctggc agtttacta gactcgcat 
      gaactgatc aaagggcaa aggcgcaaa ttatcagac 
      cctgatgtg ccccacagg attgactcc aacgcccgg 
      tctgagcca atagccatt cctccagcg gctacaagc 
      cctctagtc tcaccacac gctgatgag aaggagtta 
      tcaccaccg gtgcgaatg gtagtgggc tctgaaggc 
      tcaccccct ttagaatcc cgcgctttg attaaccca 
      tttgacccc tcacatgtc acgattaaa ttaacttcg 
      aatcgtaga cggtggact cacattttt cctaaagga 
      ccgacggga gttttgatc cagcaacac cattatcaa 
      gcgataccg gctcagcct gagccgatt aactccgaa 
      tgtgaggga aataacccg aagctggat cgcatgatt 
      tcgtccggg agtgcgcag ttggctaag tcgttttgc 
      gccactgcg gctttacac gaatattgt aagaaacgg 
      ccaaattcg cagaccaat cacagtgtg gcaagcgcc 
      cccaaaaac ccatcaaag agcctcaca ctgctgtgg 
      ggggcgacg ggtgagcag gagtggacg ccggctctg 
      acgacagga gtggattgg aagtcgctc acaataccg 
      gcctgcttg cccatcacc accgactat ttccccgac 
      aaaagggcc cttcagatt gactacgtc gtgtcttta 
      tacagttta ctgcccgat agtgtgaat gcagacttt 
      gctcaacaa cgcccaact tacaaaaga ccccttaca 
      acagttgaa gtgttcaaa gagttgatt tcgcagcgt 
      ctggcccaa ggtttccag ctaattctc atggccaac 
      aacgctgat aaaataccc agtatcaac gccctaaac 
      cccaaaatg cgggggcca gtgactcga ataagtgtg 
      accgaaaac gacgagtcg tattatctc tcaattggg 
      cgacttttc cacaaaatc tcgctgtcg ggcaatacg 
      atctcagtc accagatac agaccaaga cacccatat 
      cctgggttt aactaccaa taccggtac cgattccac 
      gccccccat cacgacact tacgaggag tcttacgct 
      actttcacc accgaaaaa ctggagcat ttcaactgg 
      aattacctc gaccattat gtgtgtaca ggtggcgac 
      accgagttc gccctagtt gatgcgctg aaatattgg 
      cgattccgc gtctacttg ctgcctttg aataattcg 
      gctaacaaa aaaatcata gacggggcc gagcactgc 
      gatatttac acagccgcc acaccgtcc gaccaccaa 
      cagatggtc gacgggctg ctgaaattc atcgaaact 
      gccgtcaat aaaataaaa cggactttg ccgtcgaag 
      aagtcgcgg aacgtcaac tgtagttca ccgttccgg 
      gagcgcctg ggcagcaac cgcctctcg gaccgcccc 
      cgtcctagg tcgggctcc aaagtcatg gagcggaat 
      cgcacctcg cagagctcg gaatcgcaa tcgagttcc 
      gagtcgcaa tcacatgat gcgttccct gacgaaaac 
      gccgtacag cccggccct tgtcttaaa aacacagcg 
      acgagttta gagatactc gaagcgatg aaaaatcct 
      ttgaatggt cttatgttt atttccgct caacagaat 
      ttaccgtcg tcgactttc atcgcttcg gatgctgtg 
      aattggctg ttgttgcgc atggaaggc gtgtcaacg 
      ctagacaaa gccgtccag ataatgcaa aatttacat 
      ttggaaggc ttcattcga cacgcttca ggtgacccg 
      aataaacag tttctattc ggtttcttc ctgttttat 
      atcattgag aaagatgat aaagatgcg tttcaacct 
      agcggcgat ttgcaaaac ttcgaaaac gagtggctg 
      gaagtcgag gtcaagtca gccctaaac tgggggctg 
      tcggacgta accaacggg gtggaagta cccaacatg 
      tactgcgac cggaaatac ggtgtcccc cacttcctg 
      tgcgaggac atcaacgtt gaatacgcc gagaacgag 
      agcaccatt tacaaaaga atgcgtttg gagactgac 
      gtcgctggt aaaagcgac cgcgtggag tggggccac 
      gtccgctac caaacgact ttcatgccg gaccaagcc 
      tacgaatta attgtcgaa tggctgaca tcatcgggt 
      tctattgtt gccgaactg ctgtccagt tggcaccgg 
      aaagcccaa acttgcggc ctgcaaatg atcccgatc 
      ccgcacgac cccctcgcc ttaccctat tctaacaaa 
      agcgacccc ttaagaggc cctattttt gtacccctt 
      aacataaaa tgcctcgag gaacgggac acaattttc 
      aaagaattc aaagacggg tcgtcgctc gaacgccta 
      tttttgctg caagagacg atcatacaa agattcggg 
      tttattaac tgcatgctg gagccgcct attaaccct 
      gaggccact tcgcgtgaa caccaatac gttcattgc 
      accggaact gtttttatt ctgatttcg cggcctgcc 
      ttaaagccc aggagcagg gtcccctca gttgggagc 
      aaacctagg tccactggg cggttctcg gtgcacgcg 
      gacgttgtg cccagcccc catgaagcc tacatcacg 
      cggcacgtc agtgggaaa aataaggac gattatgat 
      aattccaga aagatgggt tttttatgg tcttggaac 
      cacatggtc agtcgcagg tggcgcaca aaccaaggt 
      gttgataac aacttccag accaaactt ctgaaggac 
      tttagagag ttttgcgcg aatgttgac gacagattg 
      aggaacttc tgggaggag agttgggtt accaaagaa 
      aatgcttct acaaatttt agtttagac aattatatg 
      cggaaataa
    mRNA sequence
    • >TC012042 RA
      tctagtttt tatcttgtt gcaaggtgt tggtcgttg 
      cggtaactt gttgcttgt ttattgtct aacctcaaa 
      agtgacatt ttacagtga atgtcaaga cataaatta 
      tcatttttt aatcgaaat taaaatgtt aatgcgata 
      gtttgtaac ataattagt gtgttatgt tggtgggga 
      ctgttaatt ttacatttg gcacttctc gtcccccgt 
      gcgatgaaa aacaatacg atgcaaagt tcatgtgtg 
      attagttta caaaaatag aagcaaaca ataacatca 
      tgaaattgt ttcgtctta tcgtccacc agaagaatt 
      tcagtgctg aggatttaa tcatcaacc ctaaagact 
      accccggtg ctaatttgg gcgatattg tggagattt 
      accacccgc cacccgacg atgacaacc cacgtggta 
      agaagaaga gcgagcctg aggaagaat acccaaggt 
      tgttgctcc aaattaaaa cgtttccgg acgatacaa 
      cccagcaaa atgccttac aaaaagaca caatcagcg 
      tggagcaga gcatagctc aggctttcg gtctgcagg 
      cgtacaaga acgtgataa tccgtattg tggacccgc 
      ctgacattg cgcttgact caattgaac ttaccttta 
      aagaccagt acatggggc gctctgaga tgtggcgct 
      tgaaaaaca gtcttatta acacttgtg tgtacatca 
      aaaagaaca ttgagttct gtgggggcg ctgtgcgtt 
      gccaggtct acgagatgt ggtcaatgg gcgacagag 
      tcgcttgtg gggttatca cagaagaca cgaaagtcg 
      ttttccgct cctccacat ccatggttt atctcttcc 
      tgcaaatgt cgtcggaaa tgtgggact ttgatattt 
      acggcgact tgtacttcg aaaaagccg tgaacgggt 
      ttttggccg atttgttca tgaagtgga agaagaacg 
      ggagcaacc acgaagtga cgattgttt tgttttccc 
      gcacgtttt acgaagcga gtaacttgg aggcgttcc 
      ctctccaca tgcgagagt gtctccaga aggattaca 
      aaggccgat actacgagg atttttaca gggttgcgg 
      tccagaacg agcgctatg acgactgga gcgggattt 
      tggtccagt taaggcgaa ttttttccg aatatttga 
      aaattgtcc tcgagtatc acaaaaggc cagggttgg 
      atattccag ctgcgacga acagtacag cagctcagg 
      ggaattttc tagaggtgt taaacatgt ccctgaatg 
      tttttgaga agcattatt tggatcgaa gttttgata 
      ggactgggc aactcagtg ttgtggtta caccgggtg 
      ttggtgttt ttgaagttg atcgagagt tgaccaata 
      ttacgaaac agagaatta tcgataatg gggttggga 
      gtgatttgg tgtgtgttg gggaacagc cactgcacg 
      cagttccgc ttctaaagt ttcataata aagacactc 
      atatgaacg tccctgacg actactcaa tgccccact 
      ggatcaact tatcgtttt actcaacta acaaaaaaa 
      tcgcatatt cgaatttca tcccacgta ttaagctac 
      cacctcctt tacccaccg aagaggtga aaaaggatt 
      ccccgaaaa aactcgtac ctcaagacg attacattc 
      acaattcgt ttttcgatt atgacgctt acgatgcgc 
      agatttttc agttaccgc cagtgcata cagtgcacg 
      agaccagta caatacacc acaaagtaa tcccgcgtt 
      acaaacgca gctctggca gtttactag actcgcatg 
      aactgatca aagggcaaa ggcgcaaat tatcagacc 
      ctgatgtgc cccacagga ttgactcca acgcccggt 
      ctgagccaa tagccattc ctccagcgg ctacaagcc 
      ctctagtct caccacacg ctgatgaga aggagttat 
      caccaccgg tgcgaatgg tagtgggct ctgaaggct 
      caccccctt tagaatccc gcgctttga ttaacccat 
      ttgacccct cacatgtca cgattaaat taacttcga 
      atcgtagac ggtggactc acatttttc ctaaaggac 
      cgacgggag ttttgatcc agcaacacc attatcaag 
      cgataccgg ctcagcctg agccgatta actccgaat 
      gtgagggaa ataacccga agctggatc gcatgattt 
      cgtccggga gtgcgcagt tggctaagt cgttttgcg 
      ccactgcgg ctttacacg aatattgta agaaacggc 
      caaattcgc agaccaatc acagtgtgg caagcgccc 
      ccaaaaacc catcaaaga gcctcacac tgctgtggg 
      gggcgacgg gtgagcagg agtggacgc cggctctga 
      cgacaggag tggattgga agtcgctca caataccgg 
      cctgcttgc ccatcacca ccgactatt tccccgaca 
      aaagggccc ttcagattg actacgtcg tgtctttat 
      acagtttac tgcccgata gtgtgaatg cagactttg 
      ctcaacaac gcccaactt acaaaagac cccttacaa 
      cagttgaag tgttcaaag agttgattt cgcagcgtc 
      tggcccaag gtttccagc taattctca tggccaaca 
      acgctgata aaataccca gtatcaacg ccctaaacc 
      ccaaaatgc gggggccag tgactcgaa taagtgtga 
      ccgaaaacg acgagtcgt attatctct caattgggc 
      gacttttcc acaaaatct cgctgtcgg gcaatacga 
      tctcagtca ccagataca gaccaagac acccatatc 
      ctgggttta actaccaat accggtacc gattccacg 
      ccccccatc acgacactt acgaggagt cttacgcta 
      ctttcacca ccgaaaaac tggagcatt tcaactgga 
      attacctcg accattatg tgtgtacag gtggcgaca 
      ccgagttcg ccctagttg atgcgctga aatattggc 
      gattccgcg tctacttgc tgcctttga ataattcgg 
      ctaacaaaa aaatcatag acggggccg agcactgcg 
      atatttaca cagccgcca caccgtccg accaccaac 
      agatggtcg acgggctgc tgaaattca tcgaaactg 
      ccgtcaata aaataaaac ggactttgc cgtcgaaga 
      agtcgcgga acgtcaact gtagttcac cgttccggg 
      agcgcctgg gcagcaacc gcctctcgg accgccccc 
      gtcctaggt cgggctcca aagtcatgg agcggaatc 
      gcacctcgc agagctcgg aatcgcaat cgagttccg 
      agtcgcaat cacatgatg cgttccctg acgaaaacg 
      ccgtacagc ccggccctt gtcttaaaa acacagcga 
      cgagtttag agatactcg aagcgatga aaaatcctt 
      tgaatggtc ttatgttta tttccgctc aacagaatt 
      taccgtcgt cgactttca tcgcttcgg atgctgtga 
      attggctgt tgttgcgca tggaaggcg tgtcaacgc 
      tagacaaag ccgtccaga taatgcaaa atttacatt 
      tggaaggct tcattcgac acgcttcag gtgacccga 
      ataaacagt ttctattcg gtttcttcc tgttttata 
      tcattgaga aagatgata aagatgcgt ttcaaccta 
      gcggcgatt tgcaaaact tcgaaaacg agtggctgg 
      aagtcgagg tcaagtcag ccctaaact gggggctgt 
      cggacgtaa ccaacgggg tggaagtac ccaacatgt 
      actgcgacc ggaaatacg gtgtccccc acttcctgt 
      gcgaggaca tcaacgttg aatacgccg agaacgaga 
      gcaccattt acaaaagaa tgcgtttgg agactgacg 
      tcgctggta aaagcgacc gcgtggagt ggggccacg 
      tccgctacc aaacgactt tcatgccgg accaagcct 
      acgaattaa ttgtcgaat ggctgacat catcgggtt 
      ctattgttg ccgaactgc tgtccagtt ggcaccgga 
      aagcccaaa cttgcggcc tgcaaatga tcccgatcc 
      cgcacgacc ccctcgcct taccctatt ctaacaaaa 
      gcgacccct taagaggcc ctatttttg tacccctta 
      acataaaat gcctcgagg aacgggaca caattttca 
      aagaattca aagacgggt cgtcgctcg aacgcctat 
      ttttgctgc aagagacga tcatacaaa gattcgggt 
      ttattaact gcatgctgg agccgccta ttaaccctg 
      aggccactt cgcgtgaac accaatacg ttcattgca 
      ccggaactg tttttattc tgatttcgc ggcctgcct 
      taaagccca ggagcaggg tcccctcag ttgggagca 
      aacctaggt ccactgggc ggttctcgg tgcacgcgg 
      acgttgtgc ccagccccc atgaagcct acatcacgc 
      ggcacgtca gtgggaaaa ataaggacg attatgata 
      attccagaa agatgggtt ttttatggt cttggaacc 
      acatggtca gtcgcaggt ggcgcacaa accaaggtg 
      ttgataaca acttccaga ccaaacttc tgaaggact 
      ttagagagt tttgcgcga atgttgacg acagattga 
      ggaacttct gggaggaga gttgggtta ccaaagaaa 
      atgcttcta caaatttta gtttagaca attatatgc 
      ggaaataat tttatcgtg taaaataaa tttaaactt 
      cgtagcagt taattgtgt tggcgacac tgtcgtgtc 
      cataacctc acaaaaaca ttacgtaaa gaaaaacaa 
      gtcaaaact atgtcgggg gttttgaag cagacgacg 
      gggctcacg gggctggcc gtttcgaag aaccctcac 
      cacactttg ggtgtgttg tacggcaaa atcctgcga 
      accttgcag aagatgccc caggaggca gcttatagg 
      aaatacacc gaggagata gtcaacgag agggcgcag 
      gccctgaag cagaatacg acagtcgag gggattgag 
      agacaagtg ggttgtggg caagtcgag gaacttata 
      gtccaagct gagaatgaa ctgatcctg gctcgcaaa 
      atgctgacc tggaagcct tgggagccc cttctgaag 
      gaggccccc gcgaaccag tgggcctgg ccaccggcg 
      aagccgcca aaaacgtgt tagaaaatt gttgtacag 
      cttagaaaa ttagaaata aataaataa accctagtg 
      tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY