Status
Please wait ...
Gene information
General information
TC012039 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7321359..7326778
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012039
    iBeetle screen iB_05155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labrum bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs coxa size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs femur fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs tibiotarsus fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012039 RA
      MEVNPLSVI LVKSDSKGD RLLFRYPFR FLEKNEPAN 
      TKRRNPYTL PNLEDLLQS PTQPTSNIN KGHLTGFTD 
      EVLSSLFAV KSELCNNKF ELKVNDVRF VGHPTLLQL 
      RNKQQDASG LMLINVVFA LQALASHSV VTCYHDLSK 
      RVGIALRYE EKRCGYVSE QTQTMTTAH DDGYTGNSE 
      SAFETILTK CTLAKNIKK LYEDLCSTG LVNIQINKW 
      ITLSFCLPQ KVHQWHLRG KFVEPKDID RCLKALRPY 
      HSLLLLHPI QQMADFTGL DGSPSLERM LKQYSPIKN 
      LQTLAADAD LTLHHVFEL TAHLVYWAK ATVIFPICL 
      TNKYVIAPD APIHLNSPL IDKFSEAFP TANLIRVIS 
      DFALPTSLG QKCNPLCHP AQQSHLVQT IIWMLQHHL 
      LLQLHTYVQ YMPTDHGLA VPREKDERL VSQRNIGNV 
      LSSSSYHPS TSEHSRAES ESGTSTVSE TPELRSESD 
      LINRSINLE NVSFTSVED EKQDYQEEL LLDFPDEER 
      GNIFKIPAT NNPEDLRLF ARLCRKGYF HGNHHIEEI 
      MYLENLRRS QLLQLLDKF RDVLITYET EDPAIAMFS 
      YT
    CDS sequence
    • >TC012039 RA
      atggaggtg aaccctttg agtgtgatt ttggtgaaa 
      tcggacagc aagggggac cgcctctta tttcggtac 
      cccttccgg ttcttagag aaaaacgag ccggcaaac 
      accaaacgg cgaaatcct tacacgtta ccaaacctt 
      gaggaccta ctacagtcc cccactcag cccacatcg 
      aacataaat aagggccac ttgaccggt ttcactgac 
      gaagtcctg tcgtctcta tttgccgtg aagtccgaa 
      ttgtgcaat aataagttt gagctgaaa gtcaacgat 
      gtgcgattt gtgggtcac ccgactttg ctgcagttg 
      cggaataaa caacaggac gcttctggt ttaatgttg 
      attaatgtg gtgtttgct ttacaagcc cttgctagt 
      cattcagtt gtaacgtgt taccacgat ttgagtaaa 
      agagttgga attgcgtta cgttatgag gaaaaacgc 
      tgtggttat gtgagcgaa cagacgcaa accatgacc 
      acagcacac gatgatggg tacactggt aactcagaa 
      tcggccttc gagactatt ttaactaag tgtacgttg 
      gcgaaaaat attaaaaag ttgtatgaa gatttgtgc 
      agcactgga ctagtcaat attcaaatt aacaaatgg 
      atcactttg agtttttgt ttgcctcaa aaggtgcac 
      cagtggcac ttgagaggg aaattcgtt gagcctaaa 
      gacatagac aggtgtttg aaggcttta cgcccttac 
      cacagttta ttattactg caccccatc cagcaaatg 
      gccgatttc actggactg gatggctcc ccatcctta 
      gaacgaatg ctaaagcaa tattctcca ataaaaaac 
      ttgcaaact ttagcagcc gacgccgac ttgaccctc 
      caccatgtt tttgagctc acggctcat ttagtttac 
      tgggctaaa gctacagtt atattcccc atttgcttg 
      actaacaaa tatgtgata gctccagac gcccccatt 
      catctgaat tcacctctg attgacaaa ttctcagag 
      gctttcccc acagcgaat ttaattcgt gtgattagc 
      gatttcgcg ctaccgacc tctttgggc cagaagtgc 
      aacccgttg tgtcatccg gcccaacag tctcatttg 
      gtacagact attatctgg atgttgcaa caccatcta 
      ctgttgcag ctgcacact tatgtgcag tatatgcca 
      acagatcac gggttggca gtgcccagg gaaaaggac 
      gaacggctc gtttcacaa agaaacata ggaaatgta 
      ttgtcctcg agctcgtat cacccgagt acgtccgag 
      cactcgcga gccgagtcc gaaagcggc acgtcgacg 
      gtgtcggaa acgcccgag cttcgcagc gaatcagat 
      ttgatcaac cgcagtata aatctcgaa aatgtgagt 
      tttacgtcg gttgaggac gaaaaacag gattatcag 
      gaggagttg ctgttggat tttcctgat gaggaacgc 
      gggaatatt tttaaaata ccggcaact aataatcca 
      gaggatttg cgattgttt gcccgattg tgtcgcaaa 
      ggttatttt catgggaat caccacatt gaggagata 
      atgtatttg gagaactta cggcgcagt cagttgttg 
      cagctcttg gataagttt agggatgtt ttgattacg 
      tacgaaacg gaggatccg gctatagct atgttttca 
      tacacgtga
    mRNA sequence
    • >TC012039 RA
      catctgttt gctgtcatt ggcaacata accttacac 
      tttttccac atttatttc gtgtttaaa atgtgaaaa 
      ctgcacgtt tcgcaaaac tgcgactac ttaacacgc 
      cagtgagtg ctgttgttt ggtgctagt atggaggtg 
      aaccctttg agtgtgatt ttggtgaaa tcggacagc 
      aagggggac cgcctctta tttcggtac cccttccgg 
      ttcttagag aaaaacgag ccggcaaac accaaacgg 
      cgaaatcct tacacgtta ccaaacctt gaggaccta 
      ctacagtcc cccactcag cccacatcg aacataaat 
      aagggccac ttgaccggt ttcactgac gaagtcctg 
      tcgtctcta tttgccgtg aagtccgaa ttgtgcaat 
      aataagttt gagctgaaa gtcaacgat gtgcgattt 
      gtgggtcac ccgactttg ctgcagttg cggaataaa 
      caacaggac gcttctggt ttaatgttg attaatgtg 
      gtgtttgct ttacaagcc cttgctagt cattcagtt 
      gtaacgtgt taccacgat ttgagtaaa agagttgga 
      attgcgtta cgttatgag gaaaaacgc tgtggttat 
      gtgagcgaa cagacgcaa accatgacc acagcacac 
      gatgatggg tacactggt aactcagaa tcggccttc 
      gagactatt ttaactaag tgtacgttg gcgaaaaat 
      attaaaaag ttgtatgaa gatttgtgc agcactgga 
      ctagtcaat attcaaatt aacaaatgg atcactttg 
      agtttttgt ttgcctcaa aaggtgcac cagtggcac 
      ttgagaggg aaattcgtt gagcctaaa gacatagac 
      aggtgtttg aaggcttta cgcccttac cacagttta 
      ttattactg caccccatc cagcaaatg gccgatttc 
      actggactg gatggctcc ccatcctta gaacgaatg 
      ctaaagcaa tattctcca ataaaaaac ttgcaaact 
      ttagcagcc gacgccgac ttgaccctc caccatgtt 
      tttgagctc acggctcat ttagtttac tgggctaaa 
      gctacagtt atattcccc atttgcttg actaacaaa 
      tatgtgata gctccagac gcccccatt catctgaat 
      tcacctctg attgacaaa ttctcagag gctttcccc 
      acagcgaat ttaattcgt gtgattagc gatttcgcg 
      ctaccgacc tctttgggc cagaagtgc aacccgttg 
      tgtcatccg gcccaacag tctcatttg gtacagact 
      attatctgg atgttgcaa caccatcta ctgttgcag 
      ctgcacact tatgtgcag tatatgcca acagatcac 
      gggttggca gtgcccagg gaaaaggac gaacggctc 
      gtttcacaa agaaacata ggaaatgta ttgtcctcg 
      agctcgtat cacccgagt acgtccgag cactcgcga 
      gccgagtcc gaaagcggc acgtcgacg gtgtcggaa 
      acgcccgag cttcgcagc gaatcagat ttgatcaac 
      cgcagtata aatctcgaa aatgtgagt tttacgtcg 
      gttgaggac gaaaaacag gattatcag gaggagttg 
      ctgttggat tttcctgat gaggaacgc gggaatatt 
      tttaaaata ccggcaact aataatcca gaggatttg 
      cgattgttt gcccgattg tgtcgcaaa ggttatttt 
      catgggaat caccacatt gaggagata atgtatttg 
      gagaactta cggcgcagt cagttgttg cagctcttg 
      gataagttt agggatgtt ttgattacg tacgaaacg 
      gaggatccg gctatagct atgttttca tacacgtga 
      gcgtgttga ctattattc attgttttc actaattat 
      tttattaca actgttaca atcttcgta tttttgtag 
      ttaattttt tactggttg aataaagaa attacaagt 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY