Status
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Gene information
General information
TC012035 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7374062..7379422
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012035
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05154

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection


      remarks: animals lost, remarks: animals lost,
      Pupal morphology
      remarks: animals lost, remarks: animals lost,
      Adult morphology
      remarks: animals lost,
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012035 RA
        MVHALDVER IDILPGDFD TYHYKCLEG CSISFKYNR 
        ELRRHLSVK HEINIIELN LDFPNTTEF KKWLHNEET 
        ENYVQYVHL TATKRRSTG AETYYYECS RTGKRSYVD 
        DTARKRRAR KCVSKLNRG CTSQIIVNK SHDGTLSVN 
        YFKTHYGHE VDLEHVKLS KQDRCSIAS KLLLGLPVS 
        SVLHSVKNP SGSLKRINL LGRKDIVNI RKSFNIDLK 
        DGKIVNIDQ INVSQFLTE CNASEHNPV IYWSFQEFP 
        KSDELSFAL MTKYQSDML KEIGNNTVV LTSTTLNNC 
        DFELITLFV IGKSWESFP VACMFTNKV NDGTYNLFF 
        TKIKDRVGA ITPAVLITN CDQMYYEAW KSVMGEVPC 
        NYYNPVQME IDWKLNLNR IVCNETDVT RVKRKWVYD 
        SLKIFQSIG DETEFVENL DDTMKVLSK DDDFKHFLE 
        YFTRSFSDP KKWACCYRT GADLPLKQL EVINDVLRQ 
        YLNEDLETR FDVIFHSLI RFIRDETVN SMLKTKTNR 
        AFFLSENDA RHVLAMEKR LSGVHLSEN VWSVTEVES 
        GSAHIVQKY LEKKCCNLI CTTCLICLH CFSCSCVDF 
        FTGNAMCVH VHYIWVTYF KSTVQVSDD VSCDEEIVI 
        KIDEEGEEC DDHVEVLRN AIAGRTAVL NSNVANIEN 
        VGTLRLILE SVNQLCDLA QIENEEQGT EHLNDHEYL
      CDS sequence
      • >TC012035 RA
        atggttcat gctttggac gtcgaacgc attgatata 
        ctacctggg gatttcgat acctaccac tacaagtgt 
        cttgaaggc tgcagtatc tcgttcaag tacaaccgg 
        gagctgcgg aggcacctc agcgtcaaa catgagatt 
        aatataatt gagctgaat ttggatttt ccgaatact 
        actgagttt aagaagtgg ttgcacaat gaagagact 
        gagaactac gtgcagtac gtgcatttg acggccacg 
        aagcgccgg agtacgggg gcggagacc tactattat 
        gagtgtagc aggacagga aagcgcagt tacgtggac 
        gacactgcg aggaagcgc agggcgcgc aaatgtgtc 
        tccaaacta aaccgtggc tgtactagt caaataatt 
        gtaaataaa tcgcacgac ggcacatta tcggtaaat 
        tatttcaaa acacattac ggccacgag gtagattta 
        gaacacgta aaactatcc aaacaggat agatgctca 
        atcgcctca aaactcttg ctcggttta ccagtgtcg 
        tccgtttta cactcagtg aaaaaccct agtggcagt 
        ttgaaacgc atcaacctc ttggggcgc aaggacata 
        gtgaacatt cgcaaatcg ttcaacatt gatttaaaa 
        gacggcaaa atcgtgaac attgaccaa atcaacgtc 
        agtcagttt ctaacagaa tgcaacgcc tccgagcac 
        aatccggtg atttattgg agctttcag gagttcccc 
        aaatcggac gagctttcg ttcgccctc atgaccaaa 
        taccagtcc gacatgtta aaggaaatc ggtaacaac 
        accgtggtt ttgacttca acgacgcta aacaactgt 
        gatttcgaa ctgatcacg cttttcgtg atcgggaaa 
        agttgggag agtttcccc gtcgcctgc atgttcacc 
        aataaggtg aacgacggg acttacaat ttatttttt 
        acgaaaatc aaagacaga gttggcgca ataacaccg 
        gcggtttta atcacaaat tgtgaccaa atgtattac 
        gaagcctgg aagagcgta atgggggaa gtcccgtgt 
        aattattac aaccctgtc caaatggaa atcgactgg 
        aagctaaac ttgaaccgc attgtttgc aacgaaaca 
        gacgtgacc cgggtgaag cgaaaatgg gtctacgac 
        tcgctgaag attttccaa agcattggt gacgagacc 
        gagtttgtg gaaaacctg gacgacacg atgaaagtc 
        ctcagcaag gacgacgat ttcaagcac ttccttgag 
        tatttcacg aggagcttc agtgacccg aaaaagtgg 
        gcctgttgt tacagaact ggcgcagat ttgcccctc 
        aaacagctc gaagttata aacgatgtt ttgcgccag 
        tacctcaac gaggatttg gagacgcgt ttcgatgtc 
        attttccac tccctcatc aggttcatc cgggacgaa 
        accgtcaac tcgatgctg aaaaccaag acaaatcgg 
        gcgtttttc ctaagcgag aacgacgcg agacacgtg 
        ctggcgatg gaaaaaagg ctgagtggg gttcatttg 
        agcgagaac gtgtggagt gtgactgag gtggagtcc 
        gggagcgcg cacatcgtc cagaagtac ttggaaaag 
        aagtgttgc aatttaatc tgcaccact tgtctcatt 
        tgtctccat tgctttagt tgcagttgc gttgatttt 
        ttcacgggg aatgcgatg tgtgtgcac gtgcactac 
        atttgggtg acttatttc aagtcgacg gtgcaagtt 
        agtgacgat gtttcgtgc gacgaagag attgttatt 
        aagattgac gaggagggg gaggaatgt gatgaccat 
        gttgaggtt ttgcgaaat gcgattgcc gggaggact 
        gcggttttg aatagtaat gttgcgaat attgagaat 
        gtggggact ctgaggctg attttggag agtgttaat 
        cagctgtgc gatttggcg cagattgag aacgaggag 
        caagggacg gagcatttg aatgatcac gagtatttg 
        taa
      mRNA sequence
      • >TC012035 RA
        tgtcaaaaa aattgtgtg taaagaaag aatgtcttt 
        acaatgcac agagtgcag ccgagagtt ttccgtgaa 
        aagtaattt aacgcgaca ccttcgcat ggttcatgc 
        tttggacgt cgaacgcat tgatatact acctgggga 
        tttcgatac ctaccacta caagtgtct tgaaggctg 
        cagtatctc gttcaagta caaccggga gctgcggag 
        gcacctcag cgtcaaaca tgagattaa tataattga 
        gctgaattt ggattttcc gaatactac tgagtttaa 
        gaagtggtt gcacaatga agagactga gaactacgt 
        gcagtacgt gcatttgac ggccacgaa gcgccggag 
        tacgggggc ggagaccta ctattatga gtgtagcag 
        gacaggaaa gcgcagtta cgtggacga cactgcgag 
        gaagcgcag ggcgcgcaa atgtgtctc caaactaaa 
        ccgtggctg tactagtca aataattgt aaataaatc 
        gcacgacgg cacattatc ggtaaatta tttcaaaac 
        acattacgg ccacgaggt agatttaga acacgtaaa 
        actatccaa acaggatag atgctcaat cgcctcaaa 
        actcttgct cggtttacc agtgtcgtc cgttttaca 
        ctcagtgaa aaaccctag tggcagttt gaaacgcat 
        caacctctt ggggcgcaa ggacatagt gaacattcg 
        caaatcgtt caacattga tttaaaaga cggcaaaat 
        cgtgaacat tgaccaaat caacgtcag tcagtttct 
        aacagaatg caacgcctc cgagcacaa tccggtgat 
        ttattggag ctttcagga gttccccaa atcggacga 
        gctttcgtt cgccctcat gaccaaata ccagtccga 
        catgttaaa ggaaatcgg taacaacac cgtggtttt 
        gacttcaac gacgctaaa caactgtga tttcgaact 
        gatcacgct tttcgtgat cgggaaaag ttgggagag 
        tttccccgt cgcctgcat gttcaccaa taaggtgaa 
        cgacgggac ttacaattt attttttac gaaaatcaa 
        agacagagt tggcgcaat aacaccggc ggttttaat 
        cacaaattg tgaccaaat gtattacga agcctggaa 
        gagcgtaat gggggaagt cccgtgtaa ttattacaa 
        ccctgtcca aatggaaat cgactggaa gctaaactt 
        gaaccgcat tgtttgcaa cgaaacaga cgtgacccg 
        ggtgaagcg aaaatgggt ctacgactc gctgaagat 
        tttccaaag cattggtga cgagaccga gtttgtgga 
        aaacctgga cgacacgat gaaagtcct cagcaagga 
        cgacgattt caagcactt ccttgagta tttcacgag 
        gagcttcag tgacccgaa aaagtgggc ctgttgtta 
        cagaactgg cgcagattt gcccctcaa acagctcga 
        agttataaa cgatgtttt gcgccagta cctcaacga 
        ggatttgga gacgcgttt cgatgtcat tttccactc 
        cctcatcag gttcatccg ggacgaaac cgtcaactc 
        gatgctgaa aaccaagac aaatcgggc gtttttcct 
        aagcgagaa cgacgcgag acacgtgct ggcgatgga 
        aaaaaggct gagtggggt tcatttgag cgagaacgt 
        gtggagtgt gactgaggt ggagtccgg gagcgcgca 
        catcgtcca gaagtactt ggaaaagaa gtgttgcaa 
        tttaatctg caccacttg tctcatttg tctccattg 
        ctttagttg cagttgcgt tgatttttt cacggggaa 
        tgcgatgtg tgtgcacgt gcactacat ttgggtgac 
        ttatttcaa gtcgacggt gcaagttag tgacgatgt 
        ttcgtgcga cgaagagat tgttattaa gattgacga 
        ggaggggga ggaatgtga tgaccatgt tgaggtttt 
        gcgaaatgc gattgccgg gaggactgc ggttttgaa 
        tagtaatgt tgcgaatat tgagaatgt ggggactct 
        gaggctgat tttggagag tgttaatca gctgtgcga 
        tttggcgca gattgagaa cgaggagca agggacgga 
        gcatttgaa tgatcacga gtatttgta aatttttat 
        gataataaa gagatattt taatg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY