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Gene information
General information
TC012032 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7476421..7480783
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012032
    iBeetle screen iB_05153

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012032 RA
      MGKKTKIGK QRKDKYYQL AKETGFRSR AAFKLIQLN 
      RKFEFLQKS RVCIDLCAA PGGWMQVAK QNMPVSSVI 
      IGIDLFPIK PIPGCISLT EDITTEKCR VSLKKELQT 
      WKADVVLHD GAPNVGKNW LHDAYQQAC LTLSSLKLA 
      SEFLKKGGW FITKVFRSK DYHSLIWVL KQFFKKVHA 
      TKPQASRNE SAEIFVVCQ HYIAPDKID SKFMDPKYV 
      FEELEIEPK GKLNIYHPE KNKKSKAEG YPENDWTLY 
      HKIKASDFI KHPTGIELL EGASEIVFD VEEITNHEK 
      TTNEIKECC KDIKVLGRK DLKKLLAWK KALSEEFSE 
      KKDEPVEEE LEETVEKSD DEDDLKDVH QQILELESE 
      EQRELKRKK KKAQKERKK LNEKLNLKM VLKGDSGPT 
      MESDGMFEL KQVTSTKQM KKIIDQTPD TLAQSDNEE 
      DEIKSKYLR YNKDEQHLS SSGLYYKDS DSELEMESE 
      SEDEDGKIK EGLGLSDSE NSDVEEQKT KSKTKPKHP 
      LITDLDHRS KEEKKVHKA EMWFERDAF KNLIDEKDE 
      DADLDRLVS EMKKKGAKI IGEEEDAKN TESDSDYSS 
      DSDDSKYDD FEKLAPKNN VKKDGFEIV PEGQKVGKK 
      RKHKLTEEE LALGTLMIN SKKTKRDII DAAWNRYAF 
      NDDNLPDWF VKDEEKHMR KEAAVPKEL VQDYKKHLE 
      ELNVRPIKK VIEAKARKK KRALKKLEK AKKKVENLM 
      DNVDVTDRE KARQIKQLY KKARQEPKK EVTYVVAKK 
      HMAAKKMKR PAGVKGRYK VVDARMKKD LKAQKAKLK 
      TQGRRKKSK AKSK
    CDS sequence
    • >TC012032 RA
      atgggaaag aaaactaaa attgggaaa cagcgcaag 
      gataagtac tatcaattg gccaaagaa accggtttt 
      aggtctcgt gcagccttc aaattgatt caactcaac 
      cgcaagttc gagtttttg cagaagtcg cgagtatgc 
      atcgacctg tgcgccgcc cccgggggc tggatgcag 
      gtggccaag cagaacatg cccgtttcg agcgtcatc 
      attgggatt gacttgttc ccaattaag cccattcct 
      gggtgcatt tcgctgact gaggacatt acgaccgag 
      aaatgccgc gtctcgctc aagaaagag ctgcagacg 
      tggaaagcc gacgttgtt ttgcacgat ggcgcacca 
      aatgtgggc aagaattgg ctgcatgat gcctatcag 
      caggcatgc ctgacgttg agctctttg aaactggcc 
      agtgagttt ctgaagaag gggggctgg tttataacc 
      aaagtgttt cgttcgaag gattaccat tcgctgatt 
      tgggttttg aagcagttt tttaaaaaa gtgcatgcc 
      accaaacca caagcttcg cgtaatgaa tcggcggaa 
      attttcgtc gtttgtcaa cactacatc gcgcctgat 
      aaaatcgac tccaagttt atggacccg aaatatgtt 
      tttgaggag ttggaaatt gagcctaaa gggaaattg 
      aatatttac catcctgag aaaaataag aagagtaaa 
      gctgagggg taccccgag aatgattgg actttgtat 
      cataagatt aaagcatca gattttatt aaacaccct 
      acagggatc gaacttcta gaaggggct tctgagatc 
      gtttttgac gttgaagaa ataacaaat cacgaaaaa 
      actacaaac gaaattaaa gaatgttgc aaggatatt 
      aaagttttg ggaaggaaa gatttgaag aagctcttg 
      gcttggaag aaggcattg agtgaagaa ttctctgag 
      aagaaagat gaacctgtt gaagaagag cttgaagaa 
      actgtggag aaaagtgac gacgaggat gacttgaaa 
      gacgtccac caacaaata ttggaactt gaaagtgaa 
      gagcagcgc gaattgaag cgaaaaaag aaaaaagct 
      caaaaggag cgcaaaaag ctgaacgaa aaattaaac 
      ttgaagatg gtcttgaaa ggtgatagt ggccccaca 
      atggaaagt gacggcatg tttgaacta aaacaagta 
      acaagcacc aaacagatg aagaaaatt attgatcaa 
      acgcctgat actcttgct caatcagac aatgaagaa 
      gatgaaatc aaatcaaaa tatttgcgt tataacaag 
      gacgagcaa catttgagt agttcagga ttgtattat 
      aaagattca gattctgag ttggaaatg gaaagcgag 
      tctgaggac gaagatggg aaaattaag gaaggccta 
      ggtttaagc gatagcgaa aatagcgat gtggaagaa 
      cagaaaact aaatcgaaa acgaaacca aaacatcct 
      ctcataacg gacttggac cacagaagt aaggaagag 
      aaaaaagtg cacaaagcc gaaatgtgg tttgaaaga 
      gacgctttt aagaatcta atagacgag aaagacgaa 
      gacgctgat ttggaccga ctggtgagt gagatgaag 
      aagaaagga gccaagatt atcggagag gaagaagat 
      gcgaaaaac acagagtcg gatagtgac tacagttcg 
      gattcagat gattccaaa tacgacgat tttgagaaa 
      cttgcgccc aaaaataac gtaaagaag gacggtttt 
      gagattgtc cccgagggg caaaaagta ggcaagaaa 
      cgtaaacat aaattaact gaggaagaa ttggcgttg 
      ggtacgcta atgatcaac agcaaaaaa acaaaacgt 
      gacataatt gacgccgct tggaatcgt tacgctttt 
      aatgatgac aacttgcct gattggttt gtcaaagat 
      gaggagaaa catatgagg aaagaagct gcggttccc 
      aaagagttg gttcaagat tacaagaaa catttggag 
      gagttgaat gtgcgtcct attaaaaaa gtgatcgaa 
      gctaaggca aggaagaag aagagggcg ttgaagaag 
      ttggaaaaa gcgaaaaag aaggttgag aatttaatg 
      gataatgtt gatgtgacg gatcgggaa aaggcgcga 
      caaatcaaa cagttgtac aaaaaggcg agacaggag 
      ccgaagaaa gaggtgact tacgtcgtg gctaagaaa 
      cacatggct gctaaaaaa atgaagcgg ccggctgga 
      gtaaaaggg cgttataaa gtggtggac gcgcgaatg 
      aaaaaggat ttgaaagca caaaaggcc aagctaaaa 
      acccaaggc aggaggaag aaaagcaag gctaagagc 
      aagtga
    mRNA sequence
    • >TC012032 RA
      acgccatgc agtgcaatt tgtattggt tgataaaac 
      tatttttcc ccttttatc agcgaaaca tttaataat 
      aataactgg ataaaaatt ttgaaaaaa tgccaaaaa 
      accgcaaat taaaacatg tgagttggt agcattgct 
      ttttgataa cgcaaaaag gatagtcat atgtgtgtt 
      tcgtttgcc cgacaagga caacaaatt ccgaatgac 
      attctgtca aaagtcaaa agccatgtg tgtttgctg 
      aaaacaaat tagtttttt cgctttcca gaatttaaa 
      aagcgccaa catgggaaa gaaaactaa aattgggaa 
      acagcgcaa ggataagta ctatcaatt ggccaaaga 
      aaccggttt taggtctcg tgcagcctt caaattgat 
      tcaactcaa ccgcaagtt cgagttttt gcagaagtc 
      gcgagtatg catcgacct gtgcgccgc ccccggggg 
      ctggatgca ggtggccaa gcagaacat gcccgtttc 
      gagcgtcat cattgggat tgacttgtt cccaattaa 
      gcccattcc tgggtgcat ttcgctgac tgaggacat 
      tacgaccga gaaatgccg cgtctcgct caagaaaga 
      gctgcagac gtggaaagc cgacgttgt tttgcacga 
      tggcgcacc aaatgtggg caagaattg gctgcatga 
      tgcctatca gcaggcatg cctgacgtt gagctcttt 
      gaaactggc cagtgagtt tctgaagaa ggggggctg 
      gtttataac caaagtgtt tcgttcgaa ggattacca 
      ttcgctgat ttgggtttt gaagcagtt ttttaaaaa 
      agtgcatgc caccaaacc acaagcttc gcgtaatga 
      atcggcgga aattttcgt cgtttgtca acactacat 
      cgcgcctga taaaatcga ctccaagtt tatggaccc 
      gaaatatgt ttttgagga gttggaaat tgagcctaa 
      agggaaatt gaatattta ccatcctga gaaaaataa 
      gaagagtaa agctgaggg gtaccccga gaatgattg 
      gactttgta tcataagat taaagcatc agattttat 
      taaacaccc tacagggat cgaacttct agaaggggc 
      ttctgagat cgtttttga cgttgaaga aataacaaa 
      tcacgaaaa aactacaaa cgaaattaa agaatgttg 
      caaggatat taaagtttt gggaaggaa agatttgaa 
      gaagctctt ggcttggaa gaaggcatt gagtgaaga 
      attctctga gaagaaaga tgaacctgt tgaagaaga 
      gcttgaaga aactgtgga gaaaagtga cgacgagga 
      tgacttgaa agacgtcca ccaacaaat attggaact 
      tgaaagtga agagcagcg cgaattgaa gcgaaaaaa 
      gaaaaaagc tcaaaagga gcgcaaaaa gctgaacga 
      aaaattaaa cttgaagat ggtcttgaa aggtgatag 
      tggccccac aatggaaag tgacggcat gtttgaact 
      aaaacaagt aacaagcac caaacagat gaagaaaat 
      tattgatca aacgcctga tactcttgc tcaatcaga 
      caatgaaga agatgaaat caaatcaaa atatttgcg 
      ttataacaa ggacgagca acatttgag tagttcagg 
      attgtatta taaagattc agattctga gttggaaat 
      ggaaagcga gtctgagga cgaagatgg gaaaattaa 
      ggaaggcct aggtttaag cgatagcga aaatagcga 
      tgtggaaga acagaaaac taaatcgaa aacgaaacc 
      aaaacatcc tctcataac ggacttgga ccacagaag 
      taaggaaga gaaaaaagt gcacaaagc cgaaatgtg 
      gtttgaaag agacgcttt taagaatct aatagacga 
      gaaagacga agacgctga tttggaccg actggtgag 
      tgagatgaa gaagaaagg agccaagat tatcggaga 
      ggaagaaga tgcgaaaaa cacagagtc ggatagtga 
      ctacagttc ggattcaga tgattccaa atacgacga 
      ttttgagaa acttgcgcc caaaaataa cgtaaagaa 
      ggacggttt tgagattgt ccccgaggg gcaaaaagt 
      aggcaagaa acgtaaaca taaattaac tgaggaaga 
      attggcgtt gggtacgct aatgatcaa cagcaaaaa 
      aacaaaacg tgacataat tgacgccgc ttggaatcg 
      ttacgcttt taatgatga caacttgcc tgattggtt 
      tgtcaaaga tgaggagaa acatatgag gaaagaagc 
      tgcggttcc caaagagtt ggttcaaga ttacaagaa 
      acatttgga ggagttgaa tgtgcgtcc tattaaaaa 
      agtgatcga agctaaggc aaggaagaa gaagagggc 
      gttgaagaa gttggaaaa agcgaaaaa gaaggttga 
      gaatttaat ggataatgt tgatgtgac ggatcggga 
      aaaggcgcg acaaatcaa acagttgta caaaaaggc 
      gagacagga gccgaagaa agaggtgac ttacgtcgt 
      ggctaagaa acacatggc tgctaaaaa aatgaagcg 
      gccggctgg agtaaaagg gcgttataa agtggtgga 
      cgcgcgaat gaaaaagga tttgaaagc acaaaaggc 
      caagctaaa aacccaagg caggaggaa gaaaagcaa 
      ggctaagag caagtgatc atagagtta cataatttc 
      ttgtttcaa taaaataca tttttattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY