Status
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Gene information
General information
TC012016 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8469459..8472749
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012016
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05150

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012016 RA
      MKVYYNGFN LHRQFPSPQ DLVVNFIDH VYQDLENIE 
      VSYHFAVLL VANVVAFRG AKNETIPVD GKIRQISCN 
      EDKVLVLLT NGDLLKITE SVIKVPKLM GLDDDDTVV 
      QISTGSKLC VALTKKGLL YNVPNKLEF VSGDIIEVK 
      TGREHCLLL DKSGNVFSF GRGSRGQLG HGCLDDESE 
      PKFIEALGG VKIEAIEAG GWHSCALSK EGDLYVWGW 
      NGSGQLGLG GSDDGVSIM ATPQVLDFD FNIKKVGCG 
      NKHTVVLTD EGKLFGCGW NKYKQLKND ENEVFRDSD 
      KKGGMK
    CDS sequence
    • >TC012016 RA
      atgaaagtg tattacaat gggttcaac ctccatcgc 
      cagttcccc agcccacaa gacttggta gtaaatttt 
      atcgatcac gtgtaccag gacttggaa aatatcgag 
      gttagctac catttcgct gtattgttg gttgcgaac 
      gttgtggcg tttcgaggc gcgaaaaat gaaacaatt 
      cccgtggat gggaaaatt cgacaaatt tcatgcaac 
      gaagacaag gttttggtg cttttaact aatggagac 
      ttgttgaaa ataaccgaa tctgtgata aaagtgcca 
      aaattaatg ggtttggat gacgatgat accgttgtt 
      caaatttcg actggatcc aagctttgt gtagcttta 
      acgaaaaag ggactcttg tacaatgtg ccaaataag 
      ttagaattt gtaagcggg gatataatt gaggtgaaa 
      acgggccga gaacattgc ttactttta gacaaaagc 
      ggaaatgtt tttagtttt ggtagggga agtcgcggc 
      caacttggg catggatgt ctggatgat gaatctgaa 
      cctaagttt atagaagcg ttaggtggt gtgaaaatt 
      gaggctatt gaggcagga ggctggcat tcgtgtgct 
      ttgagtaaa gagggggat ttatacgtt tgggggtgg 
      aacggaagt ggacagttg ggtttaggg ggaagtgat 
      gatggggtg tccattatg gcaacgcct caagtctta 
      gattttgat tttaatatt aaaaaagtt ggctgtggt 
      aataaacat acagttgtt ttgacagat gagggaaaa 
      ttgtttggc tgtggttgg aataaatac aaacagttg 
      aagaacgac gaaaatgag gtttttcgt gactcggat 
      aagaagggc gggatgaaa taa
    mRNA sequence
    • >TC012016 RA
      tgtttgaca agtgtgtca tttacaata agaaattta 
      ttgtaaaac gcctttaat tagtattta aaccgtagt 
      tttattatt tattattaa aatcctcac catttggga 
      cctcctccg gccgaagtc aagtcaaaa aagcgagga 
      aatagctac gaaaagggt caaagttgt gagacaaca 
      ccaaacaca aaactgtca acgtaaata acttaaaca 
      aatcgatac catgaaagt gtattacaa tgggttcaa 
      cctccatcg ccagttccc cagcccaca agacttggt 
      agtaaattt tatcgatca cgtgtacca ggacttgga 
      aaatatcga ggttagcta ccatttcgc tgtattgtt 
      ggttgcgaa cgttgtggc gtttcgagg cgcgaaaaa 
      tgaaacaat tcccgtgga tgggaaaat tcgacaaat 
      ttcatgcaa cgaagacaa ggttttggt gcttttaac 
      taatggaga cttgttgaa aataaccga atctgtgat 
      aaaagtgcc aaaattaat gggtttgga tgacgatga 
      taccgttgt tcaaatttc gactggatc caagctttg 
      tgtagcttt aacgaaaaa gggactctt gtacaatgt 
      gccaaataa gttagaatt tgtaagcgg ggatataat 
      tgaggtgaa aacgggccg agaacattg cttactttt 
      agacaaaag cggaaatgt ttttagttt tggtagggg 
      aagtcgcgg ccaacttgg gcatggatg tctggatga 
      tgaatctga acctaagtt tatagaagc gttaggtgg 
      tgtgaaaat tgaggctat tgaggcagg aggctggca 
      ttcgtgtgc tttgagtaa agaggggga tttatacgt 
      ttgggggtg gaacggaag tggacagtt gggtttagg 
      gggaagtga tgatggggt gtccattat ggcaacgcc 
      tcaagtctt agattttga ttttaatat taaaaaagt 
      tggctgtgg taataaaca tacagttgt tttgacaga 
      tgagggaaa attgtttgg ctgtggttg gaataaata 
      caaacagtt gaagaacga cgaaaatga ggtttttcg 
      tgactcgga taagaaggg cgggatgaa ataaaacaa 
      ccatgaaca ttttaatgt agtccacag tataataaa 
      tactggtat acttcagaa ttacattat aaatataca 
      atgtgtatg gtatttttt tgtattttt gtggttttt 
      aaaatattt tctgaaaat aacaatttc ccacaaagc 
      aattgttca aacatctaa ctttaataa aacatgatt 
      tacaagtca cagatattt acgaacaag aacacgttg 
      tattttagg aaaaaagct agaaaactt aaaaaaata 
      taataataa aaaagtgaa aaaacgatt tgcaacaac 
      aaacaaaac ggaccgtct agccttttt cctttacat 
      gtgaaaata caacaaatt gatataata taatattca 
      cgctaaaag gcatttata gtcacaatt ttataatat 
      gagtacttt ttttaaaaa atcgtttcg tcgacaacg 
      agaccgttt cgaattcgg aaagggcct aatcgtcaa 
      aaattttaa tctaacata ttgtcaaaa catggaatc 
      atttcattt tacaaaaat tcaacaaat ttttcgtaa 
      taataaaaa acattatcc ctaattaat aatccgcac 
      cacttttta agcatagca ataagaata aaaagtttc 
      tcgagacaa aaaatatct ttctttgat ataattttt 
      gatattttg gatatgaat cagtacttt tggtcccag 
      tgtgtgcaa cggagatta atttcgtat tcatggcat 
      atttcaaaa ttatccaag gttaaattt tgatttttt 
      tcagtcttc atcaatact accgatcac atttttata 
      aaacttttt tctaccaat tttaagcac attttcgac 
      tagacaaac aaagaaacg agtatagaa gacactttt 
      tggtgttgg ttttagagc caatcgtta accttgtgt 
      ggtggtgta gagtgtggt tttgaatat gccatcttt 
      ttgtgaaaa taaataaat aaacgacct gtttgaacc 
      tgttctatc tgattttgc taccacatt gatgaagag 
      catgggccc tacatttaa actactact tttacatta 
      cgtactaag taaaatctt aaactacgt tagttaagt 
      gccatcacc tggttatta gtgtctcat ttacaaaaa 
      ctctatata gtgtggggg tatttaaac ctaacctct 
      tgtacttgt aaactgtca acaaagacg cgtaagaac 
      taccatcta tttaagtat acactctgt atattcaat 
      taatctaag gtgttttac aggggagtc tgtgaacaa 
      attgtatct tagcgcatg aaccagtcg cgacacact 
      ctatgtcgg cagaaatgt tttttactt gaagaattt 
      acgccaatt tacgtatac agcggtgct ctgataatg 
      aaaaacgac gcacccata ttgcaacat aaataacaa 
      acacaacat tatacataa aaatatcat ttcattact 
      ttttcttta cgtaaaatc cgctcttca tcaatgtga 
      ttaaatgtg ggtaaattg tgtactatt gttgtatta 
      tggttgttc caattaaca caaaaattg ctcagaggt 
      ttcaattgt aataaaaat gttctcagt ttaatctgc 
      aggcacaca aacattcaa gtgcgctga aacagtact 
      atatacacg gttgtaaag caaaaaact taagtaata 
      tttccacta cactacaca actaaaaat taattaaca 
      aatacgttg atataaaaa aaatcatta aaattatgt 
      gtttattgt aaaaaaata attaggaat aacatagaa 
      actattaga agtcttata gttgtgaaa aagctatag 
      tgtggaatg ataaatcta acatctatt tcaattaat 
      taaaaaaga ccttggtcc gttattgtt tttcttgtt 
      tattttatc ataaacgaa atactgaca cgtttggtg 
      caaaaaaga taaaaaacg aaaagtacg tccatttta 
      ataaagttt aacatgcaa acaagttaa ataataaaa 
      acaggaaaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY