Status
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Gene information
General information
TC034344
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012013
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8585566..8599940
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034344
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034344 RA
      MVRSLVQWT KTLSFPARI SPNKSSKES VISNPGSPT 
      ITPTPSNTS LSPQSKTEI NNMKNSSLN EVNQIYPDP 
      ESEKAIMSS VVYCIHHKE GCKWSDELR KLKAHLNSC 
      KHDAIPCTG KCGAQIPRV IMDEHLKTT CPQRRARCQ 
      FCNKEFTGL VFENHVGNC GYEPLYCEN KCGVKVARR 
      HLSQHKVSE CSKRLLPCR FCSKEFVAD TLSAHHLKC 
      GRVPVACPN RCDVNVLAK EDLETHLKE ECTVSVLSC 
      TYKDAGCRF KGPRYLMEK HLEESSQQH LALVCTVVT 
      KQQHQITSL KNALSRLSL NYSGTLIWK ISDFSEKMT 
      EAKNKEGME LVSPPFYTS QYGYKLQAS LFPNGNGAG 
      EDSHISVYI KILPGEYDA LLRWPFAHS VSFTLFDQS 
      SCPEKACNI VESFIPDPT WKNFQRPSK EPDSLGFGF 
      PKFVSHEML KKRHFMKDD TMFIRVKVD PSKIVAV
    CDS sequence
    • >TC034344 RA
      atggtccgc agtctagtc cagtggact aaaaccctg 
      agctttccg gcccggatc tcgcccaac aaaagctca 
      aaggagagt gtcatcagc aaccccggc agtcccaca 
      atcacgccg actcccagc aacacctcg ctgtccccg 
      caatcgaaa accgagatt aataacatg aagaatagt 
      tctctgaat gaagttaac caaatctat cccgacccg 
      gagtcggag aaggctatt atgagctct gtcgtttac 
      tgcatacac cataaggaa ggctgcaaa tggtcagat 
      gagctcaga aaattgaag gcccaccta aactcgtgc 
      aaacacgac gccatcccc tgcaccggc aaatgcggc 
      gcccaaatc ccgagagtc atcatggac gagcacctc 
      aaaaccact tgtcctcag cggcgcgcc aggtgccag 
      ttctgcaac aaggagttc accggccta gttttcgag 
      aaccacgtg ggcaactgc ggctacgag cccctctac 
      tgcgagaac aaatgcgga gtcaaagtg gcaagacgc 
      cacttgagc caacacaaa gtgagcgag tgctcgaaa 
      cgactcctc ccgtgcaga ttttgctcg aaggaattc 
      gtcgctgac acactttcc gcacaccat ttgaagtgt 
      ggaagggtt ccagtggcg tgccccaac aggtgcgac 
      gtcaacgtg ctcgcaaaa gaggatttg gagacgcac 
      ctgaaggag gaatgcacc gtgtcagtg cttagttgc 
      acctacaag gacgcagga tgccgcttt aaggggcct 
      agatactta atggagaag catttggag gaaagcagt 
      caacaacat ttagcccta gtttgcacc gttgttacg 
      aaacaacaa caccaaatc acctcactt aaaaacgcc 
      ctctccagg ctatccctc aactactct gggacccta 
      atctggaaa atcagcgac ttttccgaa aaaatgacc 
      gaagcaaag aataaagag gggatggag ttggtctcg 
      ccgcctttc tacactagt caatacgga tacaaactt 
      caggcttca ctcttcccc aatggaaac ggtgccggc 
      gaagactcc cacatttcc gtctacatt aaaatcctt 
      ccaggggaa tatgacgcc ctcttgcgc tggcccttc 
      gcccactcc gtctcgttc accctcttt gaccagtcc 
      tcctgtccg gagaaagcg tgcaatatt gtggaaagt 
      ttcatcccg gaccccacc tggaaaaac ttccaaagg 
      ccgagtaaa gagcccgat tccttgggc ttcgggttt 
      cccaaattc gtgtcacat gaaatgttg aagaagagg 
      cattttatg aaagatgac acgatgttt attagggta 
      aaagttgat cctagtaag attgttgct gtctga
    mRNA sequence
    • >TC034344 RA
      agtattgtt tgatttgtt tcgttaaaa atcacagtt 
      tagaatcct ccctgaaaa tacgttagg agctcccac 
      ctccgtgca caattacaa gcaacagct gtttttcaa 
      acacacaaa gagtgggag aaaggccat cgaaaggcc 
      gattgagta caagaagaa aacagtttg cttcgttga 
      tgcgcacgg tgcggagtg agtgtctcg aaaatggtc 
      cgcagtcta gtccagtgg actaaaacc ctgagcttt 
      ccggcccgg atctcgccc aacaaaagc tcaaaggag 
      agtgtcatc agcaacccc ggcagtccc acaatcacg 
      ccgactccc agcaacacc tcgctgtcc ccgcaatcg 
      aaaaccgag attaataac atgaagaat agttctctg 
      aatgaagtt aaccaaatc tatcccgac ccggagtcg 
      gagaaggct attatgagc tctgtcgtt tactgcata 
      caccataag gaaggctgc aaatggtca gatgagctc 
      agaaaattg aaggcccac ctaaactcg tgcaaacac 
      gacgccatc ccctgcacc ggcaaatgc ggcgcccaa 
      atcccgaga gtcatcatg gacgagcac ctcaaaacc 
      acttgtcct cagcggcgc gccaggtgc cagttctgc 
      aacaaggag ttcaccggc ctagttttc gagaaccac 
      gtgggcaac tgcggctac gagcccctc tactgcgag 
      aacaaatgc ggagtcaaa gtggcaaga cgccacttg 
      agccaacac aaagtgagc gagtgctcg aaacgactc 
      ctcccgtgc agattttgc tcgaaggaa ttcgtcgct 
      gacacactt tccgcacac catttgaag tgtggaagg 
      gttccagtg gcgtgcccc aacaggtgc gacgtcaac 
      gtgctcgca aaagaggat ttggagacg cacctgaag 
      gaggaatgc accgtgtca gtgcttagt tgcacctac 
      aaggacgca ggatgccgc tttaagggg cctagatac 
      ttaatggag aagcatttg gaggaaagc agtcaacaa 
      catttagcc ctagtttgc accgttgtt acgaaacaa 
      caacaccaa atcacctca cttaaaaac gccctctcc 
      aggctatcc ctcaactac tctgggacc ctaatctgg 
      aaaatcagc gacttttcc gaaaaaatg accgaagca 
      aagaataaa gaggggatg gagttggtc tcgccgcct 
      ttctacact agtcaatac ggatacaaa cttcaggct 
      tcactcttc cccaatgga aacggtgcc ggcgaagac 
      tcccacatt tccgtctac attaaaatc cttccaggg 
      gaatatgac gccctcttg cgctggccc ttcgcccac 
      tccgtctcg ttcaccctc tttgaccag tcctcctgt 
      ccggagaaa gcgtgcaat attgtggaa agtttcatc 
      ccggacccc acctggaaa aacttccaa aggccgagt 
      aaagagccc gattccttg ggcttcggg tttcccaaa 
      ttcgtgtca catgaaatg ttgaagaag aggcatttt 
      atgaaagat gacacgatg tttattagg gtaaaagtt 
      gatcctagt aagattgtt gctgtctga agtgtttac 
      aattttcgg ctcttgatc tcattttgt acataattg 
      taaagtttt gtaaaaagc gataagact gtatagggt 
      aacacgaaa gatgtgtgt gtgtgtgta aataaaatt 
      ggaatattt ttgcactaa aatattctc tatttctgg 
      ttagattgc tcaggaaaa ttgtaaaat tcaaaattg 
      tagtttgct ttatttttc gattttttt gtgatagct 
      ggagaagac caaagttct gtttatttc tgtgtaaat 
      ataaggcgt aaggacatg tacttcgta cgtaagcga 
      tagtcattg ttaggttag gcttctaac tagtggatc 
      atttttatt ttaataaat cctttttta cata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY