Status
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Gene information
General information
TC011998 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9039945..9055690
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011998
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05144

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011998 RA
      MASDEEGTQ LSPLNTSWR NFGTNESSG IFGESDQFT 
      RKIRTYSQW SPMEEGVTL AWNDVSVYI QTKKNGKTT 
      CKRIINGVT GAVKAGSLV ALMGASGAG KSTLMSTLA 
      HRITGGAFV EGDVLINGR PIGNYMKYI SGFMHQEDI 
      FIGSLTVSE HMNIMARLK LDRKTTQQE RNSKIHEIL 
      KSLGLTKCL NTRIGINGE SKVLSGGEK KRLAFATEL 
      LTDPPILFC DEPTTGLDS YSAQKIVTM MNTMASSGK 
      TILCTIHQP SSDIFAMFS QLILMADGR IAFIGSAAS 
      ALDFFQKAG YRCPTSYNP ADFFIKTLA TTPGFEENS 
      KQCIKRICD YFAVSDYNK EVNVVVQYE FHMGRAVEV 
      IGIMIGLCY LGTDFTTQV GIQNVEGII FLLVSENTF 
      TPMYSILDE FPQKYPLFL REYNSGLYS SFLYFLSRI 
      MAMLPGLII EPILFVIIV YWLSGLRAT TYAFLMTTL 
      AGILTLNSA AACGIFFSN AFDSVPAAM AYLVPFDYV 
      LMLTSGVFV KLSTLPRVF SWTKYLSWL MYSTESIST 
      VQWNGIKNI TCDISDQEI PCLTADTQV LEKYSFSED 
      NLSRDLWSM LFLCIIFHC LSFICLWLK IRKR
    CDS sequence
    • >TC011998 RA
      atggcttcg gacgaagaa ggtacacaa ttatctccc 
      ctaaatacg agttggcga aatttcgga actaatgaa 
      agttccgga attttcggc gaaagcgac cagttcact 
      cgaaaaatc cggacttac agccaatgg tccccgatg 
      gaggaagga gtaactctc gcctggaac gacgtttcc 
      gtctacatc caaaccaag aaaaacggc aaaaccaca 
      tgtaaacga atcataaac ggagtcacc ggtgccgta 
      aaagctgga agtttagtg gctttgatg ggagcaagt 
      ggtgccggg aaaagtaca ctaatgtca acgttagcc 
      cacagaatc accggtggc gcttttgtc gagggagat 
      gttttaatt aacggacgg cctattggc aactatatg 
      aagtatatc agcgggttt atgcaccaa gaggacatt 
      tttatcggc tctttgact gtctcagaa catatgaat 
      ataatggca aggctcaaa ttggaccgc aaaacgaca 
      caacaggag cgaaactcg aaaattcac gaaattttg 
      aaaagttta ggactcact aagtgtctc aatacgagg 
      attgggatc aatggagag agcaaagtc ttgtcagga 
      ggcgaaaaa aaacgactc gctttcgca acagaactc 
      cttacggac cccccaatt ttattctgc gacgaaccc 
      acgaccggt ctcgactca tactcagcc caaaaaatt 
      gtaacaatg atgaacaca atggcgtca agtggcaaa 
      acaatcctt tgcacaatc catcaaccc tcatctgac 
      attttcgca atgttcagt caattaatt ctgatggct 
      gatggtcga attgcgttt atcggaagt gcagcatcg 
      gctttggat tttttccaa aaggccggt tacaggtgc 
      cccacgtcg tacaatccg gccgatttc ttcatcaaa 
      acgctagca acgacgccc ggttttgag gaaaatagc 
      aaacagtgt ataaagaga atttgtgat tatttcgca 
      gtcagtgat tacaacaaa gaggtgaac gttgttgtt 
      cagtatgaa tttcacatg gggagagca gtggaggtt 
      attggaatt atgatcggt ttgtgttat ttgggcaca 
      gattttacg acacaggtt gggattcaa aatgtggaa 
      ggaattatt tttttattg gtttccgaa aatacattc 
      acaccgatg tattcgatt ttggatgag tttccacag 
      aaataccct ctgttttta agggagtac aattcaggg 
      ctgtattca tcgtttctg tatttctta tcgcggatt 
      atggcaatg ctgcctggt ttaataatc gagccgatt 
      ttgttcgtg attatcgtc tattggctt tcgggcctc 
      cgtgccacc acttatgcc ttcctaatg acaactttg 
      gcgggcatt cttaccttg aattcggcc gctgcgtgc 
      ggaatattc ttctccaat gcgttcgat tccgtgccc 
      gcagctatg gcctatttg gtgcctttt gactacgtg 
      cttatgcta acatcggga gtttttgtt aaattaagc 
      actcttcca cgggttttt tcctggacc aaatatctc 
      tcctggttg atgtattca accgaatcc atttctaca 
      gtgcaatgg aacggaata aaaaatatc acgtgcgat 
      atttccgac caggaaatc ccctgccta actgcagac 
      actcaagtc ttagaaaaa tacagtttt tcagaagac 
      aatttgtca cgtgattta tggagcatg ctgtttctc 
      tgtatcatt tttcactgt ttaagtttc atctgtttg 
      tggctgaaa atcaggaag agataa
    mRNA sequence
    • >TC011998 RA
      atggcttcg gacgaagaa ggtacacaa ttatctccc 
      ctaaatacg agttggcga aatttcgga actaatgaa 
      agttccgga attttcggc gaaagcgac cagttcact 
      cgaaaaatc cggacttac agccaatgg tccccgatg 
      gaggaagga gtaactctc gcctggaac gacgtttcc 
      gtctacatc caaaccaag aaaaacggc aaaaccaca 
      tgtaaacga atcataaac ggagtcacc ggtgccgta 
      aaagctgga agtttagtg gctttgatg ggagcaagt 
      ggtgccggg aaaagtaca ctaatgtca acgttagcc 
      cacagaatc accggtggc gcttttgtc gagggagat 
      gttttaatt aacggacgg cctattggc aactatatg 
      aagtatatc agcgggttt atgcaccaa gaggacatt 
      tttatcggc tctttgact gtctcagaa catatgaat 
      ataatggca aggctcaaa ttggaccgc aaaacgaca 
      caacaggag cgaaactcg aaaattcac gaaattttg 
      aaaagttta ggactcact aagtgtctc aatacgagg 
      attgggatc aatggagag agcaaagtc ttgtcagga 
      ggcgaaaaa aaacgactc gctttcgca acagaactc 
      cttacggac cccccaatt ttattctgc gacgaaccc 
      acgaccggt ctcgactca tactcagcc caaaaaatt 
      gtaacaatg atgaacaca atggcgtca agtggcaaa 
      acaatcctt tgcacaatc catcaaccc tcatctgac 
      attttcgca atgttcagt caattaatt ctgatggct 
      gatggtcga attgcgttt atcggaagt gcagcatcg 
      gctttggat tttttccaa aaggccggt tacaggtgc 
      cccacgtcg tacaatccg gccgatttc ttcatcaaa 
      acgctagca acgacgccc ggttttgag gaaaatagc 
      aaacagtgt ataaagaga atttgtgat tatttcgca 
      gtcagtgat tacaacaaa gaggtgaac gttgttgtt 
      cagtatgaa tttcacatg gggagagca gtggaggtt 
      attggaatt atgatcggt ttgtgttat ttgggcaca 
      gattttacg acacaggtt gggattcaa aatgtggaa 
      ggaattatt tttttattg gtttccgaa aatacattc 
      acaccgatg tattcgatt ttggatgag tttccacag 
      aaataccct ctgttttta agggagtac aattcaggg 
      ctgtattca tcgtttctg tatttctta tcgcggatt 
      atggcaatg ctgcctggt ttaataatc gagccgatt 
      ttgttcgtg attatcgtc tattggctt tcgggcctc 
      cgtgccacc acttatgcc ttcctaatg acaactttg 
      gcgggcatt cttaccttg aattcggcc gctgcgtgc 
      ggaatattc ttctccaat gcgttcgat tccgtgccc 
      gcagctatg gcctatttg gtgcctttt gactacgtg 
      cttatgcta acatcggga gtttttgtt aaattaagc 
      actcttcca cgggttttt tcctggacc aaatatctc 
      tcctggttg atgtattca accgaatcc atttctaca 
      gtgcaatgg aacggaata aaaaatatc acgtgcgat 
      atttccgac caggaaatc ccctgccta actgcagac 
      actcaagtc ttagaaaaa tacagtttt tcagaagac 
      aatttgtca cgtgattta tggagcatg ctgtttctc 
      tgtatcatt tttcactgt ttaagtttc atctgtttg 
      tggctgaaa atcaggaag agataa
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