Status
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Gene information
General information
TC031239
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011990,TC011989
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9152282..9172799
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031239
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05143

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031239 RA
      MSSGLDILS GSLRVRNGC GVGSGPYTF CRANEVKAC 
      PSLMSPETK RLLPPNTET VQTKPFPIR GERANYVNS 
      PHVIAMVGL PARGKTYIS KKLSRYLNW IGINTKVFN 
      LGEYRRHAT TAYKNHDFF RPDNQEAMA LRQQCAREA 
      LQDVCKWLE SGGEVAVFD ATNSTIERR RLIQEYVVD 
      KMGFKLFFV ESICDDPQI IEQNIKEVK VSSPDYVNM 
      NKDAVLEDF LLRIQHYQE RYQPLDEEK EKNVSFMKI 
      YNTGEKCLV HKHEGHIQA RIVYYLMNI HITPRTIYL 
      TRHGESEQN LEGRIGGDS NLSPRGRQY ASALSKYIQ 
      EQHIEGLRV WTSWLKRSI QTAAGINVP QERWKSLNE 
      IDAGVCEEM TYEEIKEKF PEEFASRDQ NKFAYRYPR 
      GESYEDLVG RLEPVIMEL ERQGNVLVV SHQAVLRCL 
      LAYFLDKSA DELPYLRVP LHTIIKLTP VAYGCRVEH 
      IKLSIDAVD THREKPKER GQNAQLVSL
    • >TC031239 RB
      MLERRRSQF QRAVSLHIG ERANYVNSP HVIAMVGLP 
      ARGKTYISK KLSRYLNWI GINTKVFNL GEYRRHATT 
      AYKNHDFFR PDNQEAMAL RQQCAREAL QDVCKWLES 
      GGEVAVFDA TNSTIERRR LIQEYVVDK MGFKLFFVE 
      SICDDPQII EQNIKEVKV SSPDYVNMN KDAVLEDFL 
      LRIQHYQER YQPLDEEKE KNVSFMKIY NTGEKCLVH 
      KHEGHIQAR IVYYLMNIH ITPRTIYLT RHGESEQNL 
      EGRIGGDSN LSPRGRQYA SALSKYIQE QHIEGLRVW 
      TSWLKRSIQ TAAGINVPQ ERWKSLNEI DAGVCEEMT 
      YEEIKEKFP EEFASRDQN KFAYRYPRG ESYEDLVGR 
      LEPVIMELE RQGNVLVVS HQAVLRCLL AYFLDKSAD 
      ELPYLRVPL HTIIKLTPV AYGCRVEHI KLSIDAVDT 
      HREKPKERG QNAQLVSL
    • >TC031239 RC
      MSSGLDILS GSLRVRNGC GVGSGPYTF CRANEVKAC 
      PSLMSPETK RLLPPNTET VQTKPFPIR GERANYVNS 
      PHVIAMVGL PARGKTYIS KKLSRYLNW IGINTKVFN 
      LGEYRRHAT TAYKNHDFF RPDNQEAMA LRQQCAREA 
      LQDVCKWLE SGGEVAVFD ATNSTIERR RLIQEYVVD 
      KMGFKLFFV ESICDDPQI IEQNIKEVK VSSPDYVNM 
      NKDAVLEDF LLRIQHYQE RYQPLDEEK EKNVSFMKI 
      YNTGEKCLV HKHEGHIQA RIVYYLMNI HITPRTIYL 
      TRHGESEQN LEGRIGGDS NLSPRGRQY ASALSKYIQ 
      EQHIEGLRV WTSWLKRSI QTAAGINVP QERWKSLNE 
      IDAGVCEEM TYEEIKEKF PEEFASRDQ NKFAYRYPR 
      GESYEDLVG RLEPVIMEL ERQGNVLVV SHQAVLRCL 
      LAYFLDKSA DELPYLRVP LHTIIKLTP VAYGCRVEH 
      IKLSIDAVD THREKPKVP GELEEKFRF KSRKT
    CDS sequence
    • >TC031239 RA
      atgagcagt ggattggat atactgagt gggtccctt 
      cgtgtgcga aacggatgc ggagtgggc tcaggccct 
      tacaccttt tgcagagcc aatgaagtc aaagcatgt 
      cccagtcta atgtcaccg gaaaccaaa aggctcctt 
      ccccccaac accgaaacg gttcaaacc aaaccgttt 
      ccgatcaga ggtgaaaga gccaactat gtcaacagc 
      ccccacgtc atcgccatg gtggggctc cctgcgcgg 
      gggaaaacc tacatctcg aagaagctg tctcggtac 
      ttaaactgg atcggcatc aataccaaa gtgttcaat 
      ttgggcgaa tatcgccgt catgcgacc accgcctac 
      aaaaaccac gatttcttc cgccccgac aaccaagaa 
      gcgatggct ttgagacag caatgtgcc agagaggcc 
      ctccaggac gtgtgcaaa tggctggaa tccgggggt 
      gaagtcgcc gtgttcgac gctaccaac tcaacaata 
      gaacgaaga aggttgatc caggaatat gttgtagac 
      aaaatgggc tttaaacta tttttcgtc gaatcgatt 
      tgtgacgat ccccaaata atcgagcaa aatatcaaa 
      gaagtgaaa gtcagcagt cctgactac gtcaatatg 
      aacaaggac gcggttttg gaagatttc ctcttacga 
      atccaacat tatcaagaa cggtatcag ccgctggat 
      gaagaaaag gaaaagaac gtttcgttt atgaagatt 
      tacaacacg ggggagaaa tgtctggta cataaacac 
      gaagggcac atccaagcg aggattgtg tactacctt 
      atgaacatc cacattacc ccgcgtacc atctatcta 
      acaaggcac ggtgaaagc gagcaaaat ctggaaggc 
      aggattgga ggcgactcc aatttgagt cccagagga 
      aggcaatac gcctccgcc ttgtccaag tacatccaa 
      gagcagcac attgaaggc ctccgagtg tggacttcg 
      tggctgaaa cgctccatc caaacagct gcgggaata 
      aacgtgcca caggaaagg tggaagtcc cttaacgaa 
      atcgatgct ggcgtatgt gaggaaatg acctatgag 
      gagattaag gagaagttc ccggaggag ttcgcctcc 
      cgggatcaa aacaaattt gcttaccga tacccccgt 
      ggtgagagt tatgaggac ctcgttgga aggcttgaa 
      cccgtaatt atggaactg gagagacag ggcaatgtg 
      ttggtggtg tcccaccag gccgtgttg cgttgtttg 
      ttggcgtat ttcttggat aaatctgct gatgaattg 
      ccttatctt cgtgttcca cttcacacc ataatcaaa 
      ttgactcca gtggcgtac gggtgtcgc gtggagcac 
      ataaaactg tccattgat gcggtcgat actcacaga 
      gagaagcca aaggaacgg ggacaaaac gcccaactg 
      gtgtcactc taa
    • >TC031239 RB
      atgcttgaa aggcgtcgc tcgcaattc caaagggcc 
      gtttcgctc cacataggt gaaagagcc aactatgtc 
      aacagcccc cacgtcatc gccatggtg gggctccct 
      gcgcggggg aaaacctac atctcgaag aagctgtct 
      cggtactta aactggatc ggcatcaat accaaagtg 
      ttcaatttg ggcgaatat cgccgtcat gcgaccacc 
      gcctacaaa aaccacgat ttcttccgc cccgacaac 
      caagaagcg atggctttg agacagcaa tgtgccaga 
      gaggccctc caggacgtg tgcaaatgg ctggaatcc 
      gggggtgaa gtcgccgtg ttcgacgct accaactca 
      acaatagaa cgaagaagg ttgatccag gaatatgtt 
      gtagacaaa atgggcttt aaactattt ttcgtcgaa 
      tcgatttgt gacgatccc caaataatc gagcaaaat 
      atcaaagaa gtgaaagtc agcagtcct gactacgtc 
      aatatgaac aaggacgcg gttttggaa gatttcctc 
      ttacgaatc caacattat caagaacgg tatcagccg 
      ctggatgaa gaaaaggaa aagaacgtt tcgtttatg 
      aagatttac aacacgggg gagaaatgt ctggtacat 
      aaacacgaa gggcacatc caagcgagg attgtgtac 
      taccttatg aacatccac attaccccg cgtaccatc 
      tatctaaca aggcacggt gaaagcgag caaaatctg 
      gaaggcagg attggaggc gactccaat ttgagtccc 
      agaggaagg caatacgcc tccgccttg tccaagtac 
      atccaagag cagcacatt gaaggcctc cgagtgtgg 
      acttcgtgg ctgaaacgc tccatccaa acagctgcg 
      ggaataaac gtgccacag gaaaggtgg aagtccctt 
      aacgaaatc gatgctggc gtatgtgag gaaatgacc 
      tatgaggag attaaggag aagttcccg gaggagttc 
      gcctcccgg gatcaaaac aaatttgct taccgatac 
      ccccgtggt gagagttat gaggacctc gttggaagg 
      cttgaaccc gtaattatg gaactggag agacagggc 
      aatgtgttg gtggtgtcc caccaggcc gtgttgcgt 
      tgtttgttg gcgtatttc ttggataaa tctgctgat 
      gaattgcct tatcttcgt gttccactt cacaccata 
      atcaaattg actccagtg gcgtacggg tgtcgcgtg 
      gagcacata aaactgtcc attgatgcg gtcgatact 
      cacagagag aagccaaag gaacgggga caaaacgcc 
      caactggtg tcactctaa
    • >TC031239 RC
      atgagcagt ggattggat atactgagt gggtccctt 
      cgtgtgcga aacggatgc ggagtgggc tcaggccct 
      tacaccttt tgcagagcc aatgaagtc aaagcatgt 
      cccagtcta atgtcaccg gaaaccaaa aggctcctt 
      ccccccaac accgaaacg gttcaaacc aaaccgttt 
      ccgatcaga ggtgaaaga gccaactat gtcaacagc 
      ccccacgtc atcgccatg gtggggctc cctgcgcgg 
      gggaaaacc tacatctcg aagaagctg tctcggtac 
      ttaaactgg atcggcatc aataccaaa gtgttcaat 
      ttgggcgaa tatcgccgt catgcgacc accgcctac 
      aaaaaccac gatttcttc cgccccgac aaccaagaa 
      gcgatggct ttgagacag caatgtgcc agagaggcc 
      ctccaggac gtgtgcaaa tggctggaa tccgggggt 
      gaagtcgcc gtgttcgac gctaccaac tcaacaata 
      gaacgaaga aggttgatc caggaatat gttgtagac 
      aaaatgggc tttaaacta tttttcgtc gaatcgatt 
      tgtgacgat ccccaaata atcgagcaa aatatcaaa 
      gaagtgaaa gtcagcagt cctgactac gtcaatatg 
      aacaaggac gcggttttg gaagatttc ctcttacga 
      atccaacat tatcaagaa cggtatcag ccgctggat 
      gaagaaaag gaaaagaac gtttcgttt atgaagatt 
      tacaacacg ggggagaaa tgtctggta cataaacac 
      gaagggcac atccaagcg aggattgtg tactacctt 
      atgaacatc cacattacc ccgcgtacc atctatcta 
      acaaggcac ggtgaaagc gagcaaaat ctggaaggc 
      aggattgga ggcgactcc aatttgagt cccagagga 
      aggcaatac gcctccgcc ttgtccaag tacatccaa 
      gagcagcac attgaaggc ctccgagtg tggacttcg 
      tggctgaaa cgctccatc caaacagct gcgggaata 
      aacgtgcca caggaaagg tggaagtcc cttaacgaa 
      atcgatgct ggcgtatgt gaggaaatg acctatgag 
      gagattaag gagaagttc ccggaggag ttcgcctcc 
      cgggatcaa aacaaattt gcttaccga tacccccgt 
      ggtgagagt tatgaggac ctcgttgga aggcttgaa 
      cccgtaatt atggaactg gagagacag ggcaatgtg 
      ttggtggtg tcccaccag gccgtgttg cgttgtttg 
      ttggcgtat ttcttggat aaatctgct gatgaattg 
      ccttatctt cgtgttcca cttcacacc ataatcaaa 
      ttgactcca gtggcgtac gggtgtcgc gtggagcac 
      ataaaactg tccattgat gcggtcgat actcacaga 
      gagaagcca aaggttcct ggtgaattg gaggaaaaa 
      ttccgattc aagtcacgc aaaacttga
    mRNA sequence
    • >TC031239 RA
      caacctgtg taaaagcac accggatcg gctcagttt 
      cgctcagtt tgtgttcgg tgccatctg gagagtata 
      cagtgtgga gtatgagca gtggattgg atatactga 
      gtgggtccc ttcgtgtgc gaaacggat gcggagtgg 
      gctcaggcc cttacacct tttgcagag ccaatgaag 
      tcaaagcat gtcccagtc taatgtcac cggaaacca 
      aaaggctcc ttcccccca acaccgaaa cggttcaaa 
      ccaaaccgt ttccgatca gaggtgaaa gagccaact 
      atgtcaaca gcccccacg tcatcgcca tggtggggc 
      tccctgcgc gggggaaaa cctacatct cgaagaagc 
      tgtctcggt acttaaact ggatcggca tcaatacca 
      aagtgttca atttgggcg aatatcgcc gtcatgcga 
      ccaccgcct acaaaaacc acgatttct tccgccccg 
      acaaccaag aagcgatgg ctttgagac agcaatgtg 
      ccagagagg ccctccagg acgtgtgca aatggctgg 
      aatccgggg gtgaagtcg ccgtgttcg acgctacca 
      actcaacaa tagaacgaa gaaggttga tccaggaat 
      atgttgtag acaaaatgg gctttaaac tatttttcg 
      tcgaatcga tttgtgacg atccccaaa taatcgagc 
      aaaatatca aagaagtga aagtcagca gtcctgact 
      acgtcaata tgaacaagg acgcggttt tggaagatt 
      tcctcttac gaatccaac attatcaag aacggtatc 
      agccgctgg atgaagaaa aggaaaaga acgtttcgt 
      ttatgaaga tttacaaca cgggggaga aatgtctgg 
      tacataaac acgaagggc acatccaag cgaggattg 
      tgtactacc ttatgaaca tccacatta ccccgcgta 
      ccatctatc taacaaggc acggtgaaa gcgagcaaa 
      atctggaag gcaggattg gaggcgact ccaatttga 
      gtcccagag gaaggcaat acgcctccg ccttgtcca 
      agtacatcc aagagcagc acattgaag gcctccgag 
      tgtggactt cgtggctga aacgctcca tccaaacag 
      ctgcgggaa taaacgtgc cacaggaaa ggtggaagt 
      cccttaacg aaatcgatg ctggcgtat gtgaggaaa 
      tgacctatg aggagatta aggagaagt tcccggagg 
      agttcgcct cccgggatc aaaacaaat ttgcttacc 
      gataccccc gtggtgaga gttatgagg acctcgttg 
      gaaggcttg aacccgtaa ttatggaac tggagagac 
      agggcaatg tgttggtgg tgtcccacc aggccgtgt 
      tgcgttgtt tgttggcgt atttcttgg ataaatctg 
      ctgatgaat tgccttatc ttcgtgttc cacttcaca 
      ccataatca aattgactc cagtggcgt acgggtgtc 
      gcgtggagc acataaaac tgtccattg atgcggtcg 
      atactcaca gagagaagc caaaggaac ggggacaaa 
      acgcccaac tggtgtcac tctaaccaa cacaataac 
      aagtcttag attagttag attagactt caaatacac 
      agtgtgttg tgtagattt gttagcaca ctgtgtctg 
      tacgcttcc tgtgcttcc ttgcttagt tgtagtttc 
      tagcaattt tgtaatcaa aatgtttta aggttcctg 
      gtgaattgg aggaaaaat tccgattca agtcacgca 
      aaacttgaa gcaattgtg cggctaatg cagatcgac 
      tgatacagg gtgcctatt ataattaaa tgaatattt 
      gatgtgatg taccaaggt ttaggttaa tataaccgt 
      ttgtgatgg agtagttag tcttttcga gatcttatt 
      gtgtacatt gttattatt atttgccat tttccaatt 
      tatatcccc tagatgtta atagttatt ggtgtaatt 
      taccataaa cgtggaata taattatct tatttttat 
      gtattagtt atttataat gtaacatat gacaatttt 
      tgcccatta taaagacaa g
    • >TC031239 RB
      gttgtttgc gttgaaaca atgcttgaa aggcgtcgc 
      tcgcaattc caaagggcc gtttcgctc cacataggt 
      gaaagagcc aactatgtc aacagcccc cacgtcatc 
      gccatggtg gggctccct gcgcggggg aaaacctac 
      atctcgaag aagctgtct cggtactta aactggatc 
      ggcatcaat accaaagtg ttcaatttg ggcgaatat 
      cgccgtcat gcgaccacc gcctacaaa aaccacgat 
      ttcttccgc cccgacaac caagaagcg atggctttg 
      agacagcaa tgtgccaga gaggccctc caggacgtg 
      tgcaaatgg ctggaatcc gggggtgaa gtcgccgtg 
      ttcgacgct accaactca acaatagaa cgaagaagg 
      ttgatccag gaatatgtt gtagacaaa atgggcttt 
      aaactattt ttcgtcgaa tcgatttgt gacgatccc 
      caaataatc gagcaaaat atcaaagaa gtgaaagtc 
      agcagtcct gactacgtc aatatgaac aaggacgcg 
      gttttggaa gatttcctc ttacgaatc caacattat 
      caagaacgg tatcagccg ctggatgaa gaaaaggaa 
      aagaacgtt tcgtttatg aagatttac aacacgggg 
      gagaaatgt ctggtacat aaacacgaa gggcacatc 
      caagcgagg attgtgtac taccttatg aacatccac 
      attaccccg cgtaccatc tatctaaca aggcacggt 
      gaaagcgag caaaatctg gaaggcagg attggaggc 
      gactccaat ttgagtccc agaggaagg caatacgcc 
      tccgccttg tccaagtac atccaagag cagcacatt 
      gaaggcctc cgagtgtgg acttcgtgg ctgaaacgc 
      tccatccaa acagctgcg ggaataaac gtgccacag 
      gaaaggtgg aagtccctt aacgaaatc gatgctggc 
      gtatgtgag gaaatgacc tatgaggag attaaggag 
      aagttcccg gaggagttc gcctcccgg gatcaaaac 
      aaatttgct taccgatac ccccgtggt gagagttat 
      gaggacctc gttggaagg cttgaaccc gtaattatg 
      gaactggag agacagggc aatgtgttg gtggtgtcc 
      caccaggcc gtgttgcgt tgtttgttg gcgtatttc 
      ttggataaa tctgctgat gaattgcct tatcttcgt 
      gttccactt cacaccata atcaaattg actccagtg 
      gcgtacggg tgtcgcgtg gagcacata aaactgtcc 
      attgatgcg gtcgatact cacagagag aagccaaag 
      gaacgggga caaaacgcc caactggtg tcactctaa 
      ccaacacaa taacaagtc ttagattag ttagattag 
      acttcaaat acacagtgt gttgtgtag atttgttag 
      cacactgtg tctgtacgc ttcctgtgc ttccttgct 
      tagttgtag tttctagca attttgtaa tcaaaatgt 
      tttaaggtt cctggtgaa ttggaggaa aaattccga 
      ttcaagtca cgcaaaact tgaagcaat tgtgcggct 
      aatgcagat cgactgata cagggtgcc tattataat 
      taaatgaat atttgatgt gatgtacca aggtttagg 
      ttaatataa ccgtttgtg atggagtag ttagtcttt 
      tcgagatct tattgtgta cattgttat tattatttg 
      ccattttcc aatttatat cccctagat gttaatagt 
      tattggtgt aatttacca taaacgtgg aatataatt 
      atcttattt ttatgtatt agttattta taatgtaac 
      atatgacaa tttttgccc attataaag acaag
    • >TC031239 RC
      caacctgtg taaaagcac accggatcg gctcagttt 
      cgctcagtt tgtgttcgg tgccatctg gagagtata 
      cagtgtgga gtatgagca gtggattgg atatactga 
      gtgggtccc ttcgtgtgc gaaacggat gcggagtgg 
      gctcaggcc cttacacct tttgcagag ccaatgaag 
      tcaaagcat gtcccagtc taatgtcac cggaaacca 
      aaaggctcc ttcccccca acaccgaaa cggttcaaa 
      ccaaaccgt ttccgatca gaggtgaaa gagccaact 
      atgtcaaca gcccccacg tcatcgcca tggtggggc 
      tccctgcgc gggggaaaa cctacatct cgaagaagc 
      tgtctcggt acttaaact ggatcggca tcaatacca 
      aagtgttca atttgggcg aatatcgcc gtcatgcga 
      ccaccgcct acaaaaacc acgatttct tccgccccg 
      acaaccaag aagcgatgg ctttgagac agcaatgtg 
      ccagagagg ccctccagg acgtgtgca aatggctgg 
      aatccgggg gtgaagtcg ccgtgttcg acgctacca 
      actcaacaa tagaacgaa gaaggttga tccaggaat 
      atgttgtag acaaaatgg gctttaaac tatttttcg 
      tcgaatcga tttgtgacg atccccaaa taatcgagc 
      aaaatatca aagaagtga aagtcagca gtcctgact 
      acgtcaata tgaacaagg acgcggttt tggaagatt 
      tcctcttac gaatccaac attatcaag aacggtatc 
      agccgctgg atgaagaaa aggaaaaga acgtttcgt 
      ttatgaaga tttacaaca cgggggaga aatgtctgg 
      tacataaac acgaagggc acatccaag cgaggattg 
      tgtactacc ttatgaaca tccacatta ccccgcgta 
      ccatctatc taacaaggc acggtgaaa gcgagcaaa 
      atctggaag gcaggattg gaggcgact ccaatttga 
      gtcccagag gaaggcaat acgcctccg ccttgtcca 
      agtacatcc aagagcagc acattgaag gcctccgag 
      tgtggactt cgtggctga aacgctcca tccaaacag 
      ctgcgggaa taaacgtgc cacaggaaa ggtggaagt 
      cccttaacg aaatcgatg ctggcgtat gtgaggaaa 
      tgacctatg aggagatta aggagaagt tcccggagg 
      agttcgcct cccgggatc aaaacaaat ttgcttacc 
      gataccccc gtggtgaga gttatgagg acctcgttg 
      gaaggcttg aacccgtaa ttatggaac tggagagac 
      agggcaatg tgttggtgg tgtcccacc aggccgtgt 
      tgcgttgtt tgttggcgt atttcttgg ataaatctg 
      ctgatgaat tgccttatc ttcgtgttc cacttcaca 
      ccataatca aattgactc cagtggcgt acgggtgtc 
      gcgtggagc acataaaac tgtccattg atgcggtcg 
      atactcaca gagagaagc caaaggttc ctggtgaat 
      tggaggaaa aattccgat tcaagtcac gcaaaactt 
      gaagcaatt gtgcggcta atgcagatc gactgatac 
      agggtgcct attataatt aaatgaata tttgatgtg 
      atgtaccaa ggtttaggt taatataac cgtttgtga 
      tggagtagt tagtctttt cgagatctt attgtgtac 
      attgttatt attatttgc cattttcca atttatatc 
      ccctagatg ttaatagtt attggtgta atttaccat 
      aaacgtgga atataatta tcttatttt tatgtatta 
      gttatttat aatgtaaca tatgacaat ttttgccca 
      ttataaaga caag
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