Status
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Gene information
General information
TC011988 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9179056..9180521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011988
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05142

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 9 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011988 RA
      MQLFKVAKG TFGQIRNLH VSASVGQNV REKKFAIYR 
      WNPDQPDKK PWVQEYKVD LNACGPMVL DALIKIKNE 
      MDPTLTFRR SCREGICGS CAMNIGGVN TLACICKID 
      TSLDKPTKI YPLPHMYVV KDLVPDMTN FYKQYKSIQ 
      PWLQRKDEK KSGEHQQYL QSMDDRQKL DGLYECILC 
      ACCSTSCPS YWWNGDKYL GPAVLMQAY RWIIDSRDD 
      ATAERLDKL KDPFSVYRC HTILNCTKT CPKGLNPGK 
      AIAEIKKLL GGLAKKDAP GLDTAALQR
    CDS sequence
    • >TC011988 RA
      atgcagttg ttcaaggtg gccaaaggt acattcgga 
      cagatccgt aatttgcat gtaagtgcg tccgtgggg 
      caaaatgtc cgcgagaag aagtttgcc atctaccgg 
      tggaacccc gaccagccc gacaagaag ccctgggtg 
      caggaatac aaggtcgat ttgaatgcc tgtggcccc 
      atggtcttg gatgcgctg attaaaatt aagaacgaa 
      atggacccc actttgacg ttcaggcgg tcgtgtcgc 
      gaagggatc tgtggctcc tgtgccatg aatataggc 
      ggggttaac acactggct tgtatttgt aaaatcgat 
      actagttta gataaaccc accaagatt tatccattg 
      ccccatatg tacgttgtt aaagatctt gtacctgat 
      atgaccaac ttctacaaa caatataag tcgatacag 
      ccctggctg cagcgcaag gatgagaag aagtcaggg 
      gaacaccag cagtacttg caaagtatg gatgaccgc 
      caaaagctc gacggtttg tatgagtgc attttgtgc 
      gcttgctgt tcaacttca tgccccagt tattggtgg 
      aacggcgat aagtatcta ggaccagct gtacttatg 
      caggcctat cgctggatc attgattcc agagatgat 
      gcaactgca gaaagattg gataaattg aaagatccg 
      ttttcagta tacagatgt cacactatc ttgaattgc 
      acaaaaacg tgtcctaaa ggtctgaac cctggaaaa 
      gctattgct gaaataaag aagttgtta ggtggtctt 
      gccaagaag gacgctccg ggacttgat actgccgct 
      ttgcaacgt taa
    mRNA sequence
    • >TC011988 RA
      atagcagga caaaacggc accaaaccg tgacacgcg 
      accggaagt cgtgaattt acatcaatt cgcaatgaa 
      aactggtct ttttcgcca attccaacg ataagtgag 
      agttcaagg tcagcacat gcggttggt aggcctgac 
      gtgttgcca acttgtcag ttttgtgtt gccagtgag 
      taaccaaaa ctgtctcga acttcgcca aaaataaag 
      caaatattt tgcgtcctt cgttgaacg cgaaaatgt 
      ctcgcgaaa tgcagttgt tcaaggtgg ccaaaggta 
      cattcggac agatccgta atttgcatg taagtgcgt 
      ccgtggggc aaaatgtcc gcgagaaga agtttgcca 
      tctaccggt ggaaccccg accagcccg acaagaagc 
      cctgggtgc aggaataca aggtcgatt tgaatgcct 
      gtggcccca tggtcttgg atgcgctga ttaaaatta 
      agaacgaaa tggacccca ctttgacgt tcaggcggt 
      cgtgtcgcg aagggatct gtggctcct gtgccatga 
      atataggcg gggttaaca cactggctt gtatttgta 
      aaatcgata ctagtttag ataaaccca ccaagattt 
      atccattgc cccatatgt acgttgtta aagatcttg 
      tacctgata tgaccaact tctacaaac aatataagt 
      cgatacagc cctggctgc agcgcaagg atgagaaga 
      agtcagggg aacaccagc agtacttgc aaagtatgg 
      atgaccgcc aaaagctcg acggtttgt atgagtgca 
      ttttgtgcg cttgctgtt caacttcat gccccagtt 
      attggtgga acggcgata agtatctag gaccagctg 
      tacttatgc aggcctatc gctggatca ttgattcca 
      gagatgatg caactgcag aaagattgg ataaattga 
      aagatccgt tttcagtat acagatgtc acactatct 
      tgaattgca caaaaacgt gtcctaaag gtctgaacc 
      ctggaaaag ctattgctg aaataaaga agttgttag 
      gtggtcttg ccaagaagg acgctccgg gacttgata 
      ctgccgctt tgcaacgtt aagtcggta tttttttgt 
      ctatactat aggcttaaa aatatattt attttgaaa 
      ctaaatttg tttttgttc ctttgagaa atgttaagt 
      tattcaaga tgtacatat ttaacgaga acttttatc 
      aaattagat ggaccttgt attatagcg attaaaaca 
      atgctttgt ggtgtaatc acgttttaa ataaacact 
      agaaaatgg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY