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Gene information
General information
TC011976 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9426071..9439670
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011976
    iBeetle screen iB_05139

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    adult cuticle darker - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment random number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    adult cuticularisation darker - Number of animals showing the phenotype: 9


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011976 RA
      MGSLPQLSI LKGPTRPLV VSQLGRIFE KVAAGPAAS 
      NIALIFEEN GQSRQLSYS QVDQITNKF ARVISETIK 
      TKNLKPNSD GDHIIAVNM HPSDHLILV LLSIWKSGC 
      AYLPLDHSF PASRIDHII QESKPVIVI YDEDSKNFP 
      AKLSYEELL TASDGRSDK RLEDKERVY QKDDLAIVL 
      YTSGSTGIP KGVRIPHKI ILNRLQWQF KTFPFSETE 
      KVCVFKTAL TFVDSVCEI WGPLISGIS ILIVPKSVT 
      LDPEKLIQK LDDYKIERL VLVPSLLRS ILMCLELKK 
      NRTLLKNLK LWVCSGETL TTSLAEEFF RYFPENEYK 
      LCNFYGSTE IMGDVTFYI ISGMQQLKN LLTVPIGAP 
      VDNTIVYLL DPELRPVKT GDIGELFVS GLNLASGYV 
      NNRDKEKFL ENQLAIDPI FSKLYRTGD FARLQNDVL 
      LYEGRTDSQ VKIRGHRVD LSEVEKAVS GVEGVEKAV 
      VLCYQPGEM NQALLAFVK SSALMNENQ IENILRSKL 
      TSYMVPQVI LVESIPLLV NGKIDRQAL LKSYENTNN 
      NDDSSVEIE IDYSGVKPA QMAAAKVLF DTVASVLNR 
      SARSAISLD SNFYEIGGN SLNSIYTIT RLNEEGYQI 
      SIGDFLSAI DLGEVLERM TSSNDIHCS PPTYTSELL 
      KNEHKSAVL DIITTSFYQ KADLEQWLM PDIFESDYK 
      ELMDALWEP LVEKALSFV AKAESGKII GVGLNFDAR 
      DEPDVQITS KLTVIFEFL ETVEGPVRD KCLPGGKGK 
      ILHSFMMGT HSSLSPKEN VAVMQFMED EVLKLATER 
      HFEGIFTTN TSPLTQQLG TDVYHYQTL LDYQVNRYV 
      ASDNTRPFG MAPDSQRAI VQWKPIKSD IVVDV
    CDS sequence
    • >TC011976 RA
      atgggttcc cttccccaa ctctcaatt ctaaaggga 
      ccaacgcgg cctttggtg gtctcccaa ctgggccga 
      attttcgaa aaagttgcg gccggccct gcagccagc 
      aacattgcc ctcatcttc gaagaaaat ggccaaagc 
      cgccaatta tcctactcg caagtcgac caaatcacg 
      aataaattt gcccgggtt ataagcgag acgatcaaa 
      acgaaaaat ctgaaaccg aattccgat ggcgatcac 
      ataattgcc gtcaatatg cacccctct gatcactta 
      atcctggtt ttgttatca atttggaaa tccggctgc 
      gcctattta cctctggac cattctttc ccggcctca 
      aggattgac cacataatc caggaatcc aagcctgtc 
      atcgttatt tatgacgaa gatagcaaa aatttcccc 
      gccaagttg tcctatgag gaattgtta actgcaagt 
      gacggaagg agtgataag agactcgag gataaagag 
      agagtttat caaaaagac gacttggct atagttttg 
      tacacgtcg ggaagtacg gggattccc aaaggagtg 
      agaatcccg cataaaatt atcctcaat cggctacag 
      tggcagttc aaaacgttc ccgttttct gaaaccgaa 
      aaagtttgc gttttcaaa accgccctc acttttgtt 
      gacagtgtt tgcgaaatc tgggggccg ctaataagc 
      ggaatttcg attttaatt gtccccaaa agtgtgact 
      cttgatcct gaaaaactt atccaaaag ctggacgat 
      tacaaaatt gagagacta gtccttgta ccctcactt 
      ttacgctca attctgatg tgtctcgag ttgaagaaa 
      aatcggacc ttgctgaaa aacctgaaa ttgtgggtt 
      tgttccggt gaaaccctc accacgagt ttagccgag 
      gagtttttc cgctatttt cccgaaaac gaatacaag 
      ttgtgcaat ttttacgga agtactgaa attatgggc 
      gatgtgaca ttttacata atttcaggg atgcaacag 
      ctgaaaaat ttgctcact gttccgatt ggtgctccg 
      gttgataac acaattgtt tacttgttg gacccggag 
      ctgcgtccg gtgaaaacc ggagacatc ggcgaactc 
      ttcgtcagc ggtctaaac ctggcgtcc ggttacgtt 
      aacaaccgc gataaggaa aagttcctg gagaaccag 
      ctcgccata gacccgatt ttctccaaa ttgtaccgc 
      actggtgat ttcgcacgt ttacaaaac gacgtgttg 
      ttgtacgaa gggaggacc gactcccag gtgaaaatc 
      cggggtcac cgagttgac ctctccgag gtcgaaaag 
      gcggttagt ggggtcgaa ggtgtggaa aaagctgtt 
      gttctgtgc taccaaccc ggagagatg aatcaagcg 
      cttttagca ttcgtcaaa agcagcgct ttgatgaac 
      gaaaatcaa atcgagaat attttgagg agtaagctg 
      acttcgtac atggttccg caagttata ttagttgaa 
      agcattcct ttgttggtt aatggcaaa atcgataga 
      caggccctg ctgaagagc tacgaaaat actaacaat 
      aacgacgat tcttctgtc gaaatcgag attgactat 
      tctggagtg aagccggcc caaatggcc gcggctaaa 
      gttctcttc gataccgtg gcttccgtt ttaaacaga 
      tcggctcgt tcggcgatt tccctcgat tcaaacttt 
      tacgaaatt gggggtaac tcactcaac tccatttac 
      accatcacc agactcaac gaagaggga tatcaaata 
      agtatcgga gatttctta tcagcgatt gatttagga 
      gaagttttg gagcgtatg acttcgtct aatgatatt 
      cattgttcg ccgccaacg tacacgtct gaactcctc 
      aaaaacgag cacaaatca gcggttctt gacattatt 
      acgacgagt ttttaccag aaggccgac ttggagcaa 
      tggctaatg ccagacatt ttcgagtcg gactataag 
      gagcttatg gacgctctt tgggagccg ctcgttgag 
      aaagcttta agtttcgtg gcgaaagca gagtcgggg 
      aaaataatc ggagtggga ctgaatttc gatgcaagg 
      gatgaaccg gatgtacaa attacttcg aaattgact 
      gtgatattt gagttcctt gaaactgtt gagggtccc 
      gtgagagac aagtgtctg ccgggcggc aaaggcaaa 
      atcttgcac tcctttatg atgggcact cattcaagt 
      ttatcgccc aaagagaat gtagcggtt atgcaattt 
      atggaagac gaagtgctt aagttggcg acagaaaga 
      cacttcgag ggaatattt acgacgaat acgagccct 
      ttgactcag caattggga accgacgtt tatcattac 
      cagacatta ctcgactat caagtaaat cgatatgtt 
      gcttcagat aatacaagg ccgtttgga atggcaccg 
      gattcacaa agagcaatc gtgcagtgg aagccaatt 
      aaaagtgac attgtagta gatgtttaa
    mRNA sequence
    • >TC011976 RA
      tagttacct tttaaacca ccggtcatc ttgttcttg 
      tgttaaacc tgtcttgga tactacaac gtgaccaaa 
      gagaaaaaa atgggttcc cttccccaa ctctcaatt 
      ctaaaggga ccaacgcgg cctttggtg gtctcccaa 
      ctgggccga attttcgaa aaagttgcg gccggccct 
      gcagccagc aacattgcc ctcatcttc gaagaaaat 
      ggccaaagc cgccaatta tcctactcg caagtcgac 
      caaatcacg aataaattt gcccgggtt ataagcgag 
      acgatcaaa acgaaaaat ctgaaaccg aattccgat 
      ggcgatcac ataattgcc gtcaatatg cacccctct 
      gatcactta atcctggtt ttgttatca atttggaaa 
      tccggctgc gcctattta cctctggac cattctttc 
      ccggcctca aggattgac cacataatc caggaatcc 
      aagcctgtc atcgttatt tatgacgaa gatagcaaa 
      aatttcccc gccaagttg tcctatgag gaattgtta 
      actgcaagt gacggaagg agtgataag agactcgag 
      gataaagag agagtttat caaaaagac gacttggct 
      atagttttg tacacgtcg ggaagtacg gggattccc 
      aaaggagtg agaatcccg cataaaatt atcctcaat 
      cggctacag tggcagttc aaaacgttc ccgttttct 
      gaaaccgaa aaagtttgc gttttcaaa accgccctc 
      acttttgtt gacagtgtt tgcgaaatc tgggggccg 
      ctaataagc ggaatttcg attttaatt gtccccaaa 
      agtgtgact cttgatcct gaaaaactt atccaaaag 
      ctggacgat tacaaaatt gagagacta gtccttgta 
      ccctcactt ttacgctca attctgatg tgtctcgag 
      ttgaagaaa aatcggacc ttgctgaaa aacctgaaa 
      ttgtgggtt tgttccggt gaaaccctc accacgagt 
      ttagccgag gagtttttc cgctatttt cccgaaaac 
      gaatacaag ttgtgcaat ttttacgga agtactgaa 
      attatgggc gatgtgaca ttttacata atttcaggg 
      atgcaacag ctgaaaaat ttgctcact gttccgatt 
      ggtgctccg gttgataac acaattgtt tacttgttg 
      gacccggag ctgcgtccg gtgaaaacc ggagacatc 
      ggcgaactc ttcgtcagc ggtctaaac ctggcgtcc 
      ggttacgtt aacaaccgc gataaggaa aagttcctg 
      gagaaccag ctcgccata gacccgatt ttctccaaa 
      ttgtaccgc actggtgat ttcgcacgt ttacaaaac 
      gacgtgttg ttgtacgaa gggaggacc gactcccag 
      gtgaaaatc cggggtcac cgagttgac ctctccgag 
      gtcgaaaag gcggttagt ggggtcgaa ggtgtggaa 
      aaagctgtt gttctgtgc taccaaccc ggagagatg 
      aatcaagcg cttttagca ttcgtcaaa agcagcgct 
      ttgatgaac gaaaatcaa atcgagaat attttgagg 
      agtaagctg acttcgtac atggttccg caagttata 
      ttagttgaa agcattcct ttgttggtt aatggcaaa 
      atcgataga caggccctg ctgaagagc tacgaaaat 
      actaacaat aacgacgat tcttctgtc gaaatcgag 
      attgactat tctggagtg aagccggcc caaatggcc 
      gcggctaaa gttctcttc gataccgtg gcttccgtt 
      ttaaacaga tcggctcgt tcggcgatt tccctcgat 
      tcaaacttt tacgaaatt gggggtaac tcactcaac 
      tccatttac accatcacc agactcaac gaagaggga 
      tatcaaata agtatcgga gatttctta tcagcgatt 
      gatttagga gaagttttg gagcgtatg acttcgtct 
      aatgatatt cattgttcg ccgccaacg tacacgtct 
      gaactcctc aaaaacgag cacaaatca gcggttctt 
      gacattatt acgacgagt ttttaccag aaggccgac 
      ttggagcaa tggctaatg ccagacatt ttcgagtcg 
      gactataag gagcttatg gacgctctt tgggagccg 
      ctcgttgag aaagcttta agtttcgtg gcgaaagca 
      gagtcgggg aaaataatc ggagtggga ctgaatttc 
      gatgcaagg gatgaaccg gatgtacaa attacttcg 
      aaattgact gtgatattt gagttcctt gaaactgtt 
      gagggtccc gtgagagac aagtgtctg ccgggcggc 
      aaaggcaaa atcttgcac tcctttatg atgggcact 
      cattcaagt ttatcgccc aaagagaat gtagcggtt 
      atgcaattt atggaagac gaagtgctt aagttggcg 
      acagaaaga cacttcgag ggaatattt acgacgaat 
      acgagccct ttgactcag caattggga accgacgtt 
      tatcattac cagacatta ctcgactat caagtaaat 
      cgatatgtt gcttcagat aatacaagg ccgtttgga 
      atggcaccg gattcacaa agagcaatc gtgcagtgg 
      aagccaatt aaaagtgac attgtagta gatgtttaa 
      gaggatttt atttatttt cagtatttt gtgtatctc 
      agaccatta ttgtaaata aaggcaatt agtgtag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY