Status
Please wait ...
Gene information
General information
TC011975 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9538751..9540443
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011975
    iBeetle screen iB_05138

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011975 RA
      MSNNVENLS PQIIRRVVK EMEDLVSNP 
      PEGIKVQIN DEDVTDIQA YIDGPAGTP 
      YMGGVFKVK LTLGKGFPQ EPPKAFFLT 
      KIFHPNVAA NGEICVNTL KKDWKSDLG 
      IKHILLTIK CLLIVPNAE SALNEEAGK 
      LLLEQYEDY SQRAKMMTE IHAQPQKIS 
      KGCSGEGPL AKKHASDKS KLTAEKKKI 
      LKDKKRTLK RL
    CDS sequence
    • >TC011975 RA
      atgtcaaac aatgtggag aatctttcg ccccagatc 
      atccgtcga gtggtcaaa gaaatggaa gatctagtc 
      tcaaatcct cccgaaggg atcaaagtt caaataaac 
      gacgaagat gtgaccgat attcaggcg tacattgac 
      ggtcctgca gggacgccg tatatgggg ggcgttttc 
      aaggtgaag ctcactctg ggcaagggg ttccctcag 
      gagccgccc aaggccttc tttctgacg aagattttc 
      cacccaaat gtggccgcc aatggcgag atttgcgtc 
      aacacgtta aaaaaggat tggaagtca gatctcgga 
      attaaacac atattactg accattaaa tgcctcctc 
      attgtgccc aacgccgaa tcagccctc aatgaagaa 
      gccgggaaa ctgctcctc gaacagtac gaagactac 
      tcacagcgg gccaaaatg atgacggaa attcacgct 
      cagccccaa aaaatttca aaaggttgc agcggggag 
      ggccctctg gctaagaag cacgccagt gataagagt 
      aaactaaca gcggagaaa aagaagata cttaaagac 
      aagaaacgg acgttgaag cgtttatga
    mRNA sequence
    • >TC011975 RA
      agggcgctt ttgaaacaa aatatgttc tttaagtgc 
      aatcttacc ttttgttgg gcgtttttt tcacttttt 
      ttaccccct gtagaatgt ggttaatgc aaagtttta 
      attttttcc taagattag gcggtttag tggctaaaa 
      tcaactttt aaatgttct aactaaact gtcgccagg 
      tgtcgcttc aaaaaattt ctaaccgct ttttgcgct 
      aattttaac gattttttg gttcatccg tcgcttttt 
      tcgtcgctg aatgtgtgt atgcgtggt gcagggcga 
      aagaaattt ttttttgag tttcatttg gcgtttttt 
      cggtgtgcc gtccccggg tccgagatt aaacgcatg 
      tgattggtg gcaaaattc aaatttcgt tgttcatct 
      gttttctct agaacgcca agtttgatg tttgttatc 
      acgattgtc aggttgttt tagcgtcgt gaaatcgcc 
      aatttagtc gatttcttg tgatatttt taattaagg 
      gcaagtgcc ggagcaatg tcaaacaat gtggagaat 
      ctttcgccc cagatcatc cgtcgagtg gtcaaagaa 
      atggaagat ctagtctca aatcctccc gaagggatc 
      aaagttcaa ataaacgac gaagatgtg accgatatt 
      caggcgtac attgacggt cctgcaggg acgccgtat 
      atggggggc gttttcaag gtgaagctc actctgggc 
      aaggggttc cctcaggag ccgcccaag gccttcttt 
      ctgacgaag attttccac ccaaatgtg gccgccaat 
      ggcgagatt tgcgtcaac acgttaaaa aaggattgg 
      aagtcagat ctcggaatt aaacacata ttactgacc 
      attaaatgc ctcctcatt gtgcccaac gccgaatca 
      gccctcaat gaagaagcc gggaaactg ctcctcgaa 
      cagtacgaa gactactca cagcgggcc aaaatgatg 
      acggaaatt cacgctcag ccccaaaaa atttcaaaa 
      ggttgcagc ggggagggc cctctggct aagaagcac 
      gccagtgat aagagtaaa ctaacagcg gagaaaaag 
      aagatactt aaagacaag aaacggacg ttgaagcgt 
      ttatgaaaa ccgttactg ccattcttg tttatttct 
      ttgttttaa ctatttttt tttaattat taaattata 
      ttttttgag tatgcaaga atgatttat taatttata 
      tgtgtgcta cagtttggt tgagacctt ttcatttcg 
      tgttatttt gaatgtagt gcatgcaca aagtgcaat 
      ttattcatg gttccaatc aaaaaataa atgttaggc 
      tcagttggt tttccacca gtcaattac aaagtgtct 
      gcaacaaat attgaaaca aaccggaac attgttggc 
      ctcaacatc aaacatagc tgagcctta ggatgtatt 
      tgtggtaga gaattgtga attatttat taataaaaa 
      ctgaaaatc aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY