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Gene information
General information
TC011968 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10611918..10618235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011968
    iBeetle screen iB_05136

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011968 RA
      MKLWLCLII SVLSYVPEI ECLDQIVYA INCGGEAHT 
      DSHGIRYER DPLHGKIGI ASDYGKRLL IGRVPPNDH 
      ILYQTERYH TNTFGYDIP VNADGEYVL VLKFSEVYF 
      NAPNQKVFD VVLNGDHTI VSDLDIFEK VGRGVAHDE 
      YIPFKISKG RLIYGSEES EIRGSKIRV EFIKGYKDN 
      PKINAMYVM RGKVDDVPK LPPIPVEPD PIEEYKEEP 
      EVVPKNLRP SGPKQPDPY AMDDSSVML PVFIAIGAF 
      IPLLFCLCK L
    CDS sequence
    • >TC011968 RA
      atgaaattg tggttatgt ttgataata agtgtccta 
      agttacgtt cccgaaata gaatgttta gatcaaatt 
      gtttatgcc ataaactgt ggaggtgag gcacacaca 
      gactcccat gggattcgc tacgagagg gacccattg 
      catggtaaa attgggatt gcatcagac tacggcaaa 
      cggttgtta attggaaga gtgccacca aacgaccac 
      attttatat caaactgaa aggtatcac acaaatacc 
      tttggatac gacatccct gtgaatgcc gacggggag 
      tatgtcctt gttttgaag ttttccgaa gtgtatttc 
      aatgcaccc aaccaaaaa gtgttcgat gtcgtcctt 
      aatggagac cacaccatt gtctccgat ttggatatt 
      tttgagaaa gttgggcga ggagttgcc cacgacgag 
      tacataccg ttcaaaata tcaaagggg cgcctgatt 
      tatggtagt gaagagtct gagatacga ggctccaaa 
      ataagggtg gaatttatt aaaggttac aaggataac 
      ccaaaaatc aacgcaatg tacgtaatg cgagggaaa 
      gttgatgac gtacccaaa ctaccaccg atacctgtg 
      gaacctgat ccaatcgaa gagtacaag gaagaacct 
      gaagtggtt ccgaaaaac cttagacct agtggaccc 
      aaacaacct gatccatat gccatggat gattcttca 
      gttatgtta cctgttttt atagcaatt ggagcgttt 
      ataccttta ctattttgt ctttgcaaa ttgtga
    mRNA sequence
    • >TC011968 RA
      aagttttgg attatttgc caaaatgtt tgatataac 
      ctctcatgc tgttaagaa aactaattc gtttcgtat 
      ctcaatcat aatggaaga aatttttga aatatttag 
      ctgtccctt tatattatt cttaacttt tatataaat 
      gtctcgttg tactaaata acatacagg gtggctcaa 
      ttcgcgcaa acgaagtac acagttata ggtgagtaa 
      agatcgaat gtttatcca acaccctgt atttaccga 
      gtgagactg gtgtaaacg gaggtcgaa ttttgcgca 
      tttcagttt acttttgtc gaaactgtt acgttctgg 
      cggatgggt gcggaaaaa gatattcta ataaccgaa 
      attcacgtt gcagtaaaa ttcttaaaa atgttcgtt 
      gccatcata ccagaaatc acgcacaca ataaaatat 
      caaaaagat ttttaaact gaatgcttt taatttctg 
      atcaaagta gcccatgac attgaaatt aggttggca 
      acgctggct gtcaaatta aaatgtcac tgtcatttt 
      gacagaaat acaagcatg gtacctttt atatagttg 
      tataaaact aagggagcg aacataaat attttgtct 
      gtgatttat cacttcatt ttacggtaa atgtgtatc 
      tatttgtaa gtagcttta gggtagaat tatttactg 
      agtgtcttg tctttacaa ctgttaaag ttctacatc 
      tgtgtaatg tactttgtc aataatgtg tcagtttac 
      atttttatt gcatttgta acggtgcgt agtcaagtt 
      aaaatgtca atttaatcg aagtttgtt cacaatgtt 
      ttgcttgca attattcca tcttctaca cagccgatt 
      tggtagata gggaacgtc gcagcctac tttgttttt 
      ttaaacggt gttttaata aaaagccgc gagatgtcg 
      cccaggtag gtggacctg tctgaagtt gaagcaaat 
      gaaaaaaga gaaatgttc agtttccta tacatcaag 
      gtggctggg ttttttgca tcttgaata ttacgcaaa 
      tatatttga cgttttttt taatgttgt taaatcgta 
      taccaaaat gtacaataa aacagaata tcgagaagc 
      agcaggttt ggggacgta tgaaaaaaa ttagctgaa 
      gtacttagt cttattaca agactttcc agaaaactc 
      aataatgca gcttaagat gaaaacgtt ttccgtatt 
      ttgacatgt tttcgcaat cttatcttg gttagtatt 
      tggagttga cataaattt aaacgaatt gaatggcgt 
      caaattcaa agtttttat tttttgtta ttctattat 
      tatttttat ttcagttgt tatttgtgg tcttaagct 
      gcattgtaa gtagtcgta atatttctt ctgagttat 
      agttaccaa tgattttgt ttttcctta gtacaagta 
      ttttcttag tatggtatt ggagacact ttgttataa 
      acatatctt agtgcatat ttcatggta tttgttctt 
      ttttcacga tgttttggg tgcgaagtt tgtataaag 
      atggttgtt ataaaggcc taaatcgtt tctttctat 
      aaatgttgt tataaaaat agtattgtt atagtgtgt 
      tgcattgca ttcttatag gcgtcttaa ttatgtagt 
      tcttataga tgtaaaata aaaaggaat cgaatttcg 
      tgttttctt tatctcctt tatccttat atttgatag 
      acttcttta catgaaatt acgttctac gttttttaa 
      tgtgtaaaa gccacccaa tattgtaat ttttgctaa 
      agttttcct caaactagc agcccagac ttggcaaca 
      ttgctatag agggcgcga tcggcgctt gaatcatga 
      cagatcgat aacgaccta acctcacaa aactgagtg 
      atagaagta aagggcaaa tgtgccggt gataaatat 
      attctaaat attgccacg tttatgatt gatgcaata 
      acaagtgat ccctcagaa gtttgcaat ttatcatga 
      aattgtggt tatgtttga taataagtg tcctaagtt 
      acgttcccg aaatagaat gtttagatc aaattgttt 
      atgccataa actgtggag gtgaggcac acacagact 
      cccatggga ttcgctacg agagggacc cattgcatg 
      gtaaaattg ggattgcat cagactacg gcaaacggt 
      tgttaattg gaagagtgc caccaaacg accacattt 
      tatatcaaa ctgaaaggt atcacacaa atacctttg 
      gatacgaca tccctgtga atgccgacg gggagtatg 
      tccttgttt tgaagtttt ccgaagtgt atttcaatg 
      cacccaacc aaaaagtgt tcgatgtcg tccttaatg 
      gagaccaca ccattgtct ccgatttgg atatttttg 
      agaaagttg ggcgaggag ttgcccacg acgagtaca 
      taccgttca aaatatcaa aggggcgcc tgatttatg 
      gtagtgaag agtctgaga tacgaggct ccaaaataa 
      gggtggaat ttattaaag gttacaagg ataacccaa 
      aaatcaacg caatgtacg taatgcgag ggaaagttg 
      atgacgtac ccaaactac caccgatac ctgtggaac 
      ctgatccaa tcgaagagt acaaggaag aacctgaag 
      tggttccga aaaacctta gacctagtg gacccaaac 
      aacctgatc catatgcca tggatgatt cttcagtta 
      tgttacctg tttttatag caattggag cgtttatac 
      ctttactat tttgtcttt gcaaattgt gatccttgt 
      tttttttaa cacaatgtg attatgcca gtgttattt 
      tcagccttt tttatgtat agaaaaagt ggtttagta 
      ctggttaaa gggattttt taatgagtg taaatattt 
      acagtatta tcagtttgc tgggtactt atttatgtc 
      atccttttt tttgctgat tgttaaaga tacgatata 
      gaagtgcat aaataaaga ctttatagc taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY