| General information | |||
| TC011963 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 10716203..10725186 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011963 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05134 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC011963 RA
MDTGLLNLE EHFSTYYVC EPDEQSCSD DNNRSYFNE WEDERELNG DIGLIPEPS SFCGSLKGM SDITDSPPS LLFQKMIDW STSIYVDGV DAFPPEEAV SHHFTDQDV EQKLQTNLA TSELSSCFN DKDVNNFDL AKYINESAF TDSRKVKSV GKKIDKQAQ KYIIEDKDD NFDTSDEVD VETVFEGAS LPVLEANDA FSLLEQFEA SEKPPLANA KHNENLSSD FGFSSSLVN NLEMDVNHI SSSSIEIFD KIPKAPIDI FDEDVNLFE DDSQTVKES PQTEILDKP KEQEKSKDE EVKTKQEAK TTKPQPSES NTSHNYTKN KQILDALPQ ELIARINES GKRKTITVI PPIPAKKRG ASRSTESVS IHRNKIVKV AVNEQVHFD HDYCATPSP YPKEPQKDS GFQSSEEDD RSVIRNQPT VKTADGKLM VSLLKVNTI RSNNHNQKK KLNLEEYKK RRLINSNAT SQSNSPASS TCSSPLPED ENLKRLKHQ EKLMKMAAD LLNTPAKST KTDVALPAL KPVVQNPPP QRIVVPQKI TPPPDLEVK TYVSIGTNT DISSINCNK TILASTQQL EEIKPLLQK VSNKINSNS FITSLIENI PKVKTKTTA FVKSENQEV VREDKIIHY LKKDREPVK TVSVGSQTD FDEGVGDDM ESRYRRRID RSPSVSSSG SSRTRHRRR RSSSSSSRS SHSSYSSSS SSTSSRDNY GFVTFANKE DAYEAVEHG NDGHVYPKY DLSFGGRRI FCKTSYSDL DNMREDDYN YYTSRAPEN SFDALLRAA QEKIRKRKP
- >TC011963 RB
MDTGLLNLE EHFSTYYVC EPDEQSCSD DNNRSYFNE WEDERELNG DIGLIPEPS SFCGSLKGM SDITDSPPS LLFQKMIDW STSIYVDGV DAFPPEEAV SHHFTDQDV EQKLQTNLA TSELSSCFN DKDVNNFDL AKYINESAF TDSRKVKSV GKKIDKQAQ KYIIEDKDD NFDTSDEVD VETVFEGAS LPVLEANDA FSLLEQFEA SEKPPLANA KHNENLSSD FGFSSSLVN NLEMDVNHI SSSSIEIFD KIPKAPIDI FDEDVNLFE DDSQTVKES PQTEILDKP KEQEKSKDE EVKTKQEAK TTKPQPSES NTSHNYTKN KQILDALPQ ELIARINES GKRKTITVI PPIPAKKRG ASRSTESVS IHRNKIVKV AVNEQVHFD HDYCATPSP YPKEPQKDS GFQSSEEDD RSVIRNQPT VKTADGKLM VSLLKVNTI RSNNHNQKK KLNLEEYKK RRLINSNAT SQSNSPASS TCSSPLPED ENLKRLKHQ EKLMKMAAD LLNTPAKST KTDVALPAL KPVVQNPPP QRIVVPQKI TPPPDLEVK TYVSIGTNT DISSINCNK TILASTQQL EEIKPLLQK VSNKINSNS FITSLIENI PKVKTKTTA FVKSENQEV VREDKIIHY LKKDREPVK TVSVGSQTD FDEGVGDDM ESRYRRRID RSPSVSSSG SSRTRHRRR RSSSSSSRS SHSSYSSSS SSTSSRSSR SRSRSISPK RQRQYSAER LREVEERRV IYVGRITKG TTKEDLRRR FITFGPITN VSLHFRDYG DNYGFVTFA NKEDAYEAV EHGNDGHVY PKYDLSFGG RRIFCKTSY SDLDNMRED DYNYYTSRA PENSFDALL RAAQEKIRK RKP
- >TC011963 RA
atggatacg ggtctcttg aatttagag gagcatttt tccacatat tacgtgtgt gagccggac gaacaatcg tgctcagac gacaacaac agatcgtat ttcaatgaa tgggaggac gaaagagag ctgaatggc gatattggt ctgatacca gagccaagc agtttttgt ggctccttg aagggcatg tccgatata acggattca cctccttcg ctacttttt caaaagatg attgactgg tccacttcc atttatgtg gacggtgtc gatgccttt cctccggaa gaggcggtc tcgcatcat tttacggat caagacgtc gaacaaaag ttacaaaca aatttagct actagcgaa ttatctagt tgttttaat gataaagat gtaaataat tttgattta gcgaaatac attaacgaa tctgcattc accgacagc agaaaagtt aaatcagtg ggcaaaaaa attgataaa caagcccaa aaatacata attgaagat aaagacgat aattttgat acgagtgat gaagttgat gttgaaact gtatttgaa ggtgctagt cttccggta ttagaggcg aacgatgct ttcagtctt ctagaacaa ttcgaagca tccgaaaag cctcctcta gccaatgct aaacacaac gagaatctg agtagtgat tttggattt agtagtagt ttagtcaat aacctagaa atggatgtt aatcacatt tcaagtagc tcgattgag atattcgat aaaattcca aaagctccc atcgatatc tttgatgag gatgttaat ttatttgag gatgattcg caaactgtt aaagagtct cctcaaaca gaaattttg gataagcct aaagaacaa gaaaaaagt aaggatgaa gaagttaaa accaagcaa gaagccaaa actacgaaa ccacaacct tcggaatca aacacttcg cataattac actaaaaat aaacagata ttagacgca ctacctcaa gagttgatt gcgcgaatc aacgaatca gggaaacgc aaaaccata actgttatt cccccgatt cctgctaaa aaacggggc gcttcgcga tctactgaa tcagtatca attcaccgt aacaagatt gtaaaagtg gccgttaac gaacaagta cattttgac catgattat tgtgccacg ccatctccc taccccaag gaaccccaa aaagactca ggttttcag tcatcggaa gaggatgac cgttctgta ataagaaac caaccgact gttaaaaca gccgatggt aaactcatg gtgtcgctt ctcaaagtg aacacaata cgtagcaat aatcacaat cagaagaaa aaattgaat ctggaagag tacaaaaag aggcgtttg attaattcc aatgcaacg tctcagagt aactctcct gctagtagt acatgcagt tcccccttg cctgaagac gagaatttg aaacgtctg aaacaccaa gagaagttg atgaaaatg gccgctgat ttgttaaat actccggca aaatcaaca aaaaccgac gttgctctt cctgctctt aaaccagta gttcagaat cctccacct cagagaatt gtcgttccg cagaaaata acacctcct ccagaccta gaagtgaag acgtatgtc agtattggc accaatacg gatatttcc agtatcaac tgtaataaa acgattctc gcgtccaca caacaattg gaagagatc aagccgttg ttgcagaaa gttagcaat aaaattaat agcaattcg tttattaca tcgcttata gaaaatatc ccgaaagtt aagacaaaa actacagcg tttgttaag tcggaaaat caggaggtg gttcgagaa gataagatc attcattat ttgaagaaa gacagagag ccggttaaa acggtcagt gtagggtcg caaaccgat tttgatgaa ggggtaggg gatgatatg gaatcgagg tacaggcga aggatagat aggagcccg agtgtttcg tccagtggg agttcacgg actcgacat cgcagaaga cgcagtagt tcgagttct tcacgtagt tcacatagt tcatattcc tcatcatca tcatccact tcttctagg gataattac ggatttgtg acttttgcg aacaaggaa gacgcctat gaagctgta gagcatggc aatgacggc catgtttac cccaagtat gatttgagt tttggtggt cgaaggata ttttgcaaa acttcctat tctgattta gataatatg cgggaagac gattataat tattacact tctcgcgct cctgaaaat tcgttcgat gctttgttg agggcagca caagagaaa atacgtaaa cggaagcct tga
- >TC011963 RB
atggatacg ggtctcttg aatttagag gagcatttt tccacatat tacgtgtgt gagccggac gaacaatcg tgctcagac gacaacaac agatcgtat ttcaatgaa tgggaggac gaaagagag ctgaatggc gatattggt ctgatacca gagccaagc agtttttgt ggctccttg aagggcatg tccgatata acggattca cctccttcg ctacttttt caaaagatg attgactgg tccacttcc atttatgtg gacggtgtc gatgccttt cctccggaa gaggcggtc tcgcatcat tttacggat caagacgtc gaacaaaag ttacaaaca aatttagct actagcgaa ttatctagt tgttttaat gataaagat gtaaataat tttgattta gcgaaatac attaacgaa tctgcattc accgacagc agaaaagtt aaatcagtg ggcaaaaaa attgataaa caagcccaa aaatacata attgaagat aaagacgat aattttgat acgagtgat gaagttgat gttgaaact gtatttgaa ggtgctagt cttccggta ttagaggcg aacgatgct ttcagtctt ctagaacaa ttcgaagca tccgaaaag cctcctcta gccaatgct aaacacaac gagaatctg agtagtgat tttggattt agtagtagt ttagtcaat aacctagaa atggatgtt aatcacatt tcaagtagc tcgattgag atattcgat aaaattcca aaagctccc atcgatatc tttgatgag gatgttaat ttatttgag gatgattcg caaactgtt aaagagtct cctcaaaca gaaattttg gataagcct aaagaacaa gaaaaaagt aaggatgaa gaagttaaa accaagcaa gaagccaaa actacgaaa ccacaacct tcggaatca aacacttcg cataattac actaaaaat aaacagata ttagacgca ctacctcaa gagttgatt gcgcgaatc aacgaatca gggaaacgc aaaaccata actgttatt cccccgatt cctgctaaa aaacggggc gcttcgcga tctactgaa tcagtatca attcaccgt aacaagatt gtaaaagtg gccgttaac gaacaagta cattttgac catgattat tgtgccacg ccatctccc taccccaag gaaccccaa aaagactca ggttttcag tcatcggaa gaggatgac cgttctgta ataagaaac caaccgact gttaaaaca gccgatggt aaactcatg gtgtcgctt ctcaaagtg aacacaata cgtagcaat aatcacaat cagaagaaa aaattgaat ctggaagag tacaaaaag aggcgtttg attaattcc aatgcaacg tctcagagt aactctcct gctagtagt acatgcagt tcccccttg cctgaagac gagaatttg aaacgtctg aaacaccaa gagaagttg atgaaaatg gccgctgat ttgttaaat actccggca aaatcaaca aaaaccgac gttgctctt cctgctctt aaaccagta gttcagaat cctccacct cagagaatt gtcgttccg cagaaaata acacctcct ccagaccta gaagtgaag acgtatgtc agtattggc accaatacg gatatttcc agtatcaac tgtaataaa acgattctc gcgtccaca caacaattg gaagagatc aagccgttg ttgcagaaa gttagcaat aaaattaat agcaattcg tttattaca tcgcttata gaaaatatc ccgaaagtt aagacaaaa actacagcg tttgttaag tcggaaaat caggaggtg gttcgagaa gataagatc attcattat ttgaagaaa gacagagag ccggttaaa acggtcagt gtagggtcg caaaccgat tttgatgaa ggggtaggg gatgatatg gaatcgagg tacaggcga aggatagat aggagcccg agtgtttcg tccagtggg agttcacgg actcgacat cgcagaaga cgcagtagt tcgagttct tcacgtagt tcacatagt tcatattcc tcatcatca tcatccact tcttctaga tcgtctcgt tcacgatcg agatccatt tctccgaaa cgtcaacgg caatacagc gccgaacgt ttaagagaa gttgaagaa cgtagagtt atttatgtg ggccgtatc acgaaaggt accacaaaa gaggattta cggaggcga ttcattacg ttcggaccc attacgaac gttagtctc catttccga gactatggg gataattac ggatttgtg acttttgcg aacaaggaa gacgcctat gaagctgta gagcatggc aatgacggc catgtttac cccaagtat gatttgagt tttggtggt cgaaggata ttttgcaaa acttcctat tctgattta gataatatg cgggaagac gattataat tattacact tctcgcgct cctgaaaat tcgttcgat gctttgttg agggcagca caagagaaa atacgtaaa cggaagcct tga
- >TC011963 RA
cgacatttg ccgatttgg agaaatttg cggtgtggt tagtcgctc acgtgtaca ttgttgtgc ttttcaccc aaatattta ttaaagttt gtgttaaac agtgttgaa aacatgtaa ataccgtag cgagacaat cagtgcttc gatttcacc aagaataat caaagtgta ctgttaaaa acaaaaggt gttaacaaa aatatgtaa gctggtgtt cataaccta taatcatct tgaactatt aaaagctta ttattacgt aaataagct gaataattg tatgtagtt ttgcctgga aggcgaaat tgtgaggaa aatttcgtc tagaccaca ttgttagaa cagttcctt atttattag cagtcttgt tgagtggca gtgttattg accgcaaat atggatacg ggtctcttg aatttagag gagcatttt tccacatat tacgtgtgt gagccggac gaacaatcg tgctcagac gacaacaac agatcgtat ttcaatgaa tgggaggac gaaagagag ctgaatggc gatattggt ctgatacca gagccaagc agtttttgt ggctccttg aagggcatg tccgatata acggattca cctccttcg ctacttttt caaaagatg attgactgg tccacttcc atttatgtg gacggtgtc gatgccttt cctccggaa gaggcggtc tcgcatcat tttacggat caagacgtc gaacaaaag ttacaaaca aatttagct actagcgaa ttatctagt tgttttaat gataaagat gtaaataat tttgattta gcgaaatac attaacgaa tctgcattc accgacagc agaaaagtt aaatcagtg ggcaaaaaa attgataaa caagcccaa aaatacata attgaagat aaagacgat aattttgat acgagtgat gaagttgat gttgaaact gtatttgaa ggtgctagt cttccggta ttagaggcg aacgatgct ttcagtctt ctagaacaa ttcgaagca tccgaaaag cctcctcta gccaatgct aaacacaac gagaatctg agtagtgat tttggattt agtagtagt ttagtcaat aacctagaa atggatgtt aatcacatt tcaagtagc tcgattgag atattcgat aaaattcca aaagctccc atcgatatc tttgatgag gatgttaat ttatttgag gatgattcg caaactgtt aaagagtct cctcaaaca gaaattttg gataagcct aaagaacaa gaaaaaagt aaggatgaa gaagttaaa accaagcaa gaagccaaa actacgaaa ccacaacct tcggaatca aacacttcg cataattac actaaaaat aaacagata ttagacgca ctacctcaa gagttgatt gcgcgaatc aacgaatca gggaaacgc aaaaccata actgttatt cccccgatt cctgctaaa aaacggggc gcttcgcga tctactgaa tcagtatca attcaccgt aacaagatt gtaaaagtg gccgttaac gaacaagta cattttgac catgattat tgtgccacg ccatctccc taccccaag gaaccccaa aaagactca ggttttcag tcatcggaa gaggatgac cgttctgta ataagaaac caaccgact gttaaaaca gccgatggt aaactcatg gtgtcgctt ctcaaagtg aacacaata cgtagcaat aatcacaat cagaagaaa aaattgaat ctggaagag tacaaaaag aggcgtttg attaattcc aatgcaacg tctcagagt aactctcct gctagtagt acatgcagt tcccccttg cctgaagac gagaatttg aaacgtctg aaacaccaa gagaagttg atgaaaatg gccgctgat ttgttaaat actccggca aaatcaaca aaaaccgac gttgctctt cctgctctt aaaccagta gttcagaat cctccacct cagagaatt gtcgttccg cagaaaata acacctcct ccagaccta gaagtgaag acgtatgtc agtattggc accaatacg gatatttcc agtatcaac tgtaataaa acgattctc gcgtccaca caacaattg gaagagatc aagccgttg ttgcagaaa gttagcaat aaaattaat agcaattcg tttattaca tcgcttata gaaaatatc ccgaaagtt aagacaaaa actacagcg tttgttaag tcggaaaat caggaggtg gttcgagaa gataagatc attcattat ttgaagaaa gacagagag ccggttaaa acggtcagt gtagggtcg caaaccgat tttgatgaa ggggtaggg gatgatatg gaatcgagg tacaggcga aggatagat aggagcccg agtgtttcg tccagtggg agttcacgg actcgacat cgcagaaga cgcagtagt tcgagttct tcacgtagt tcacatagt tcatattcc tcatcatca tcatccact tcttctagg gataattac ggatttgtg acttttgcg aacaaggaa gacgcctat gaagctgta gagcatggc aatgacggc catgtttac cccaagtat gatttgagt tttggtggt cgaaggata ttttgcaaa acttcctat tctgattta gataatatg cgggaagac gattataat tattacact tctcgcgct cctgaaaat tcgttcgat gctttgttg agggcagca caagagaaa atacgtaaa cggaagcct tgacaaagt ttgtggttt agtgctaaa tttcataag ctcataatt catcgcatt tttttattg atttgattg tattttatt ctgtatttt ttagttttg attgttata gcatgatgg taacatgac aacctctta ttataaaat taatatgtt cggcttcta caagtggtc ggttttaat catgaaaac acaacaaaa aaggactga tttttaaag tgaatttgt gattgtatt tttattcga tttaagttt aatttgggt tagaaatta gtaaatttg aaataaatg atccactgt ttatttgcc aatggttga aaccgactg ttccgttgt tggatatgt ttcccacat tgggctgtg tattttgta tttacaaat attttgtta cagtttaat aaacgttaa caaagctc
- >TC011963 RB
cgacatttg ccgatttgg agaaatttg cggtgtggt tagtcgctc acgtgtaca ttgttgtgc ttttcaccc aaatattta ttaaagttt gtgttaaac agtgttgaa aacatgtaa ataccgtag cgagacaat cagtgcttc gatttcacc aagaataat caaagtgta ctgttaaaa acaaaaggt gttaacaaa aatatgtaa gctggtgtt cataaccta taatcatct tgaactatt aaaagctta ttattacgt aaataagct gaataattg tatgtagtt ttgcctgga aggcgaaat tgtgaggaa aatttcgtc tagaccaca ttgttagaa cagttcctt atttattag cagtcttgt tgagtggca gtgttattg accgcaaat atggatacg ggtctcttg aatttagag gagcatttt tccacatat tacgtgtgt gagccggac gaacaatcg tgctcagac gacaacaac agatcgtat ttcaatgaa tgggaggac gaaagagag ctgaatggc gatattggt ctgatacca gagccaagc agtttttgt ggctccttg aagggcatg tccgatata acggattca cctccttcg ctacttttt caaaagatg attgactgg tccacttcc atttatgtg gacggtgtc gatgccttt cctccggaa gaggcggtc tcgcatcat tttacggat caagacgtc gaacaaaag ttacaaaca aatttagct actagcgaa ttatctagt tgttttaat gataaagat gtaaataat tttgattta gcgaaatac attaacgaa tctgcattc accgacagc agaaaagtt aaatcagtg ggcaaaaaa attgataaa caagcccaa aaatacata attgaagat aaagacgat aattttgat acgagtgat gaagttgat gttgaaact gtatttgaa ggtgctagt cttccggta ttagaggcg aacgatgct ttcagtctt ctagaacaa ttcgaagca tccgaaaag cctcctcta gccaatgct aaacacaac gagaatctg agtagtgat tttggattt agtagtagt ttagtcaat aacctagaa atggatgtt aatcacatt tcaagtagc tcgattgag atattcgat aaaattcca aaagctccc atcgatatc tttgatgag gatgttaat ttatttgag gatgattcg caaactgtt aaagagtct cctcaaaca gaaattttg gataagcct aaagaacaa gaaaaaagt aaggatgaa gaagttaaa accaagcaa gaagccaaa actacgaaa ccacaacct tcggaatca aacacttcg cataattac actaaaaat aaacagata ttagacgca ctacctcaa gagttgatt gcgcgaatc aacgaatca gggaaacgc aaaaccata actgttatt cccccgatt cctgctaaa aaacggggc gcttcgcga tctactgaa tcagtatca attcaccgt aacaagatt gtaaaagtg gccgttaac gaacaagta cattttgac catgattat tgtgccacg ccatctccc taccccaag gaaccccaa aaagactca ggttttcag tcatcggaa gaggatgac cgttctgta ataagaaac caaccgact gttaaaaca gccgatggt aaactcatg gtgtcgctt ctcaaagtg aacacaata cgtagcaat aatcacaat cagaagaaa aaattgaat ctggaagag tacaaaaag aggcgtttg attaattcc aatgcaacg tctcagagt aactctcct gctagtagt acatgcagt tcccccttg cctgaagac gagaatttg aaacgtctg aaacaccaa gagaagttg atgaaaatg gccgctgat ttgttaaat actccggca aaatcaaca aaaaccgac gttgctctt cctgctctt aaaccagta gttcagaat cctccacct cagagaatt gtcgttccg cagaaaata acacctcct ccagaccta gaagtgaag acgtatgtc agtattggc accaatacg gatatttcc agtatcaac tgtaataaa acgattctc gcgtccaca caacaattg gaagagatc aagccgttg ttgcagaaa gttagcaat aaaattaat agcaattcg tttattaca tcgcttata gaaaatatc ccgaaagtt aagacaaaa actacagcg tttgttaag tcggaaaat caggaggtg gttcgagaa gataagatc attcattat ttgaagaaa gacagagag ccggttaaa acggtcagt gtagggtcg caaaccgat tttgatgaa ggggtaggg gatgatatg gaatcgagg tacaggcga aggatagat aggagcccg agtgtttcg tccagtggg agttcacgg actcgacat cgcagaaga cgcagtagt tcgagttct tcacgtagt tcacatagt tcatattcc tcatcatca tcatccact tcttctaga tcgtctcgt tcacgatcg agatccatt tctccgaaa cgtcaacgg caatacagc gccgaacgt ttaagagaa gttgaagaa cgtagagtt atttatgtg ggccgtatc acgaaaggt accacaaaa gaggattta cggaggcga ttcattacg ttcggaccc attacgaac gttagtctc catttccga gactatggg gataattac ggatttgtg acttttgcg aacaaggaa gacgcctat gaagctgta gagcatggc aatgacggc catgtttac cccaagtat gatttgagt tttggtggt cgaaggata ttttgcaaa acttcctat tctgattta gataatatg cgggaagac gattataat tattacact tctcgcgct cctgaaaat tcgttcgat gctttgttg agggcagca caagagaaa atacgtaaa cggaagcct tgacaaagt ttgtggttt agtgctaaa tttcataag ctcataatt catcgcatt tttttattg atttgattg tattttatt ctgtatttt ttagttttg attgttata gcatgatgg taacatgac aacctctta ttataaaat taatatgtt cggcttcta caagtggtc ggttttaat catgaaaac acaacaaaa aaggactga tttttaaag tgaatttgt gattgtatt tttattcga tttaagttt aatttgggt tagaaatta gtaaatttg aaataaatg atccactgt ttatttgcc aatggttga aaccgactg ttccgttgt tggatatgt ttcccacat tgggctgtg tattttgta tttacaaat attttgtta cagtttaat aaacgttaa caaagctc
