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Gene information
General information
TC011960 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10764869..10849773
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011960
    iBeetle screen iB_05133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg not present - Number of animals showing the phenotype: 2

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased -

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011960 RA
      MFLDDENNT RLNNPSCSW SGSARATIF VIILPAATS 
      ATSSDLYHF PGAENATSM ETLYEPWNC TKLPCNFEN 
      ETLAHNDTN DTLSDATRG EPLADLILM GFLSVVLGL 
      MILVTVIGN VFVIAAILL ERNLQNVAN YLIVSLAVA 
      DLMVACLVM PLGAVYAIS NNWIMGPEL CDMWTSIDV 
      LCCTASILH LVAIALDRY WAVTNVDYI HTRNSHRIG 
      IMIVVVWSI ALVVSLAPQ IPQFKDPEY LKRIQEKHQ 
      CLVSQDLKY QIFATSSTF YVPLLVIFV LYWKIFQTA 
      RKRIRRRRE MKGQVVLKV EKTNNNSKV SKNFLTKRK 
      FLKMKKCSS NKKSSAAEA LVSSLVMME GQSTTTMEI 
      VEEQDEVQS NGKQEETTA FTITKPQTS TLVQSNAEV 
      PLVSNNVSP EKTSSATTN NGSASHQSH ISDISRVEI 
      LNKEIPPCP ASVDKLIPS RREKKESLE AKRERKAAK 
      TLAIITGAF VMCWLPFFI LALLMPLCG AACTISEYI 
      SSFALWLGY FNSTLNPVI YTIFNPEFR QAFKRILCG 
      GHSRGRNFR SGKIR
    CDS sequence
    • >TC011960 RA
      atgttcctc gacgacgaa aataataca aggctaaat 
      aatccctcc tgcagctgg agcggctct gccagagcc 
      acaatcttc gtcatcatc ctgccagcg gccacttcc 
      gccacgtct tcagatctg taccacttt cccggggca 
      gagaacgcc acctcgatg gagaccctc tacgagccg 
      tggaactgc acgaaactg ccctgcaat ttcgagaac 
      gaaacgctg gcccataac gacaccaac gacacgctc 
      agcgacgcc accagaggc gagcctttg gcggacctc 
      attcttatg ggatttctg tcagttgtg ctcggtctc 
      atgatactg gtcacggtg ataggtaac gttttcgtg 
      atagcagcc atattactg gagcgaaac ttgcaaaac 
      gtggcgaat tacctgatc gtgtctttg gcagtggcg 
      gacttgatg gtggcgtgc cttgttatg ccgctgggt 
      gccgtgtat gcgataagt aacaattgg attatgggt 
      ccggagttg tgcgatatg tggacttca atcgacgtc 
      ctgtgttgc acggcatca attttgcat ctggtcgcc 
      atcgccctt gatcgatac tgggccgta acgaatgta 
      gattacatc cacacaaga aacagccac cgaatcggt 
      atcatgatc gtggttgtt tggagtatt gctttggtg 
      gtttcactc gcaccgcag ataccgcaa tttaaagac 
      cccgaatac ttgaagagg atccaggaa aagcatcaa 
      tgcttagtt agccaggat ctcaagtac caaattttc 
      gcaactagt tccacgttt tacgttcct ctcttagtc 
      attttcgtt ctgtattgg aagatcttt caaacggcg 
      aggaagcgc atcaggaga cgacgggaa atgaagggg 
      caagtcgtc ttaaaggtc gagaaaacg aacaataac 
      agtaaggtg tcgaagaat ttcctcacg aagaggaaa 
      ttcctcaaa atgaaaaag tgttcaagc aacaagaag 
      tcttccgct gctgaggct ctggtctcg tcgctcgtc 
      atgatggag ggccagtcc accaccacg atggaaatc 
      gtcgaggag caggacgag gtccagagc aacggcaaa 
      caagaggaa acgacagct ttcacgata acgaagccg 
      caaacatcg accctagtt cagagcaac gccgaggtc 
      ccgttggtc tccaacaac gtctccccc gaaaaaacc 
      tcgtcggcg acgaccaac aacggctcg gcgagccac 
      caaagccac atcagcgat attagtaga gtagaaatc 
      ttaaataag gaaataccc ccgtgtccg gcgtccgtc 
      gacaagctg ataccgtcc aggagggag aagaaggag 
      agcctagag gcgaaacgc gagcgcaaa gcggcaaaa 
      accctggcc atcattacc ggagcgttc gtgatgtgc 
      tggttgccg tttttcatc ctggcactg cttatgccc 
      ctgtgcggg gccgcgtgc accatcagc gagtacatc 
      tcgagcttc gccctctgg ctgggctat ttcaactcg 
      acgctgaac ccggtcatt tacacgatc ttcaacccg 
      gagttcaga caggccttc aagaggatc ctgtgtgga 
      ggacacagc agagggcgc aatttccgc tcgggcaaa 
      attagataa
    mRNA sequence
    • >TC011960 RA
      ttaaattta ggtgaattg ttgaccaca aactgtcgc 
      aaacttgca ttttcctcg ccacgacat gaaaactag 
      attgtcaga agtccgaaa tgttcctcg acgacgaaa 
      ataatacaa ggctaaata atccctcct gcagctgga 
      gcggctctg ccagagcca caatcttcg tcatcatcc 
      tgccagcgg ccacttccg ccacgtctt cagatctgt 
      accactttc ccggggcag agaacgcca cctcgatgg 
      agaccctct acgagccgt ggaactgca cgaaactgc 
      cctgcaatt tcgagaacg aaacgctgg cccataacg 
      acaccaacg acacgctca gcgacgcca ccagaggcg 
      agcctttgg cggacctca ttcttatgg gatttctgt 
      cagttgtgc tcggtctca tgatactgg tcacggtga 
      taggtaacg ttttcgtga tagcagcca tattactgg 
      agcgaaact tgcaaaacg tggcgaatt acctgatcg 
      tgtctttgg cagtggcgg acttgatgg tggcgtgcc 
      ttgttatgc cgctgggtg ccgtgtatg cgataagta 
      acaattgga ttatgggtc cggagttgt gcgatatgt 
      ggacttcaa tcgacgtcc tgtgttgca cggcatcaa 
      ttttgcatc tggtcgcca tcgcccttg atcgatact 
      gggccgtaa cgaatgtag attacatcc acacaagaa 
      acagccacc gaatcggta tcatgatcg tggttgttt 
      ggagtattg ctttggtgg tttcactcg caccgcaga 
      taccgcaat ttaaagacc ccgaatact tgaagagga 
      tccaggaaa agcatcaat gcttagtta gccaggatc 
      tcaagtacc aaattttcg caactagtt ccacgtttt 
      acgttcctc tcttagtca ttttcgttc tgtattgga 
      agatctttc aaacggcga ggaagcgca tcaggagac 
      gacgggaaa tgaaggggc aagtcgtct taaaggtcg 
      agaaaacga acaataaca gtaaggtgt cgaagaatt 
      tcctcacga agaggaaat tcctcaaaa tgaaaaagt 
      gttcaagca acaagaagt cttccgctg ctgaggctc 
      tggtctcgt cgctcgtca tgatggagg gccagtcca 
      ccaccacga tggaaatcg tcgaggagc aggacgagg 
      tccagagca acggcaaac aagaggaaa cgacagctt 
      tcacgataa cgaagccgc aaacatcga ccctagttc 
      agagcaacg ccgaggtcc cgttggtct ccaacaacg 
      tctcccccg aaaaaacct cgtcggcga cgaccaaca 
      acggctcgg cgagccacc aaagccaca tcagcgata 
      ttagtagag tagaaatct taaataagg aaatacccc 
      cgtgtccgg cgtccgtcg acaagctga taccgtcca 
      ggagggaga agaaggaga gcctagagg cgaaacgcg 
      agcgcaaag cggcaaaaa ccctggcca tcattaccg 
      gagcgttcg tgatgtgct ggttgccgt ttttcatcc 
      tggcactgc ttatgcccc tgtgcgggg ccgcgtgca 
      ccatcagcg agtacatct cgagcttcg ccctctggc 
      tgggctatt tcaactcga cgctgaacc cggtcattt 
      acacgatct tcaacccgg agttcagac aggccttca 
      agaggatcc tgtgtggag gacacagca gagggcgca 
      atttccgct cgggcaaaa ttagataaa aatatcagt 
      tcctggcag gctggcgca ggatttttg gttcgcaat 
      aaaaccggc aacattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY