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Gene information
General information
TC011938 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11369451..11379454
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011938
    iBeetle screen iB_05131

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna proximal shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna scapus bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna scapus thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011938 RA
      MAHLVTSVI DKYEDILEN KSDPRVKGW AMMSSPMPT 
      LCICLFYAY FSTVLGPRI MENRKPFNL RKTLIFYNL 
      FQTLFSAWI FYEYLMSGW WGSYSFRCQ PVDYSNSPM 
      ALRMARTCW WYYFSKFTE FFDTLFFIL RKKNSHVST 
      LHVIHHGCM PFSVWMGMK FAPGGHSTF FALLNTFVH 
      IIMYFYYMV AAMGPQYQK YIWWKKYLT TFQMVQFVA 
      IFTHQFQLL FTECNYPKS FMIWIALHG VMFLFLFSD 
      FYKVRYTNN KAVKSNSGA CMPVLEDEK QQNGTVTKS 
      DSNGTLNNT YIKSEYSDS YKACYSNGS TNGFVSQTV 
      IERNSKKIN
    CDS sequence
    • >TC011938 RA
      atggctcat ctcgtgacc agcgtcatc gacaaatac 
      gaggacatt ctagaaaac aaaagtgac ccgagggtc 
      aaaggatgg gccatgatg tctagtccc atgccaacg 
      ctgtgcatt tgcctattt tatgcgtac ttttcgacg 
      gttctagga ccccggatt atggaaaat aggaaaccg 
      tttaattta agaaagact ttaattttt tataattta 
      tttcaaact ctcttcagc gcgtggata ttttacgag 
      tacttgatg agcggctgg tgggggagc tacagcttc 
      aggtgccaa cctgtcgac tactcaaac agccccatg 
      gcgctcagg atggcgcgg acctgctgg tggtactac 
      ttttcgaag ttcaccgaa tttttcgac actctattt 
      ttcattttg cggaagaaa aatagccac gtgtccact 
      ctccacgtc atccaccac ggctgcatg ccgttttcg 
      gtctggatg gggatgaaa ttcgcgcca ggtggccac 
      agcacgttc ttcgccttg ctcaacaca ttcgtgcac 
      atcatcatg tatttctac tacatggtg gcggccatg 
      gggccccag taccagaaa tacatctgg tggaagaaa 
      tacctgacc acgttccag atggtgcag tttgtggcg 
      attttcacc caccagttc cagctgctc ttcaccgag 
      tgcaattac ccgaagagc ttcatgatt tggatcgct 
      ttgcacggt gtcatgttc ttattcctg ttctccgat 
      ttttacaaa gttaggtac accaacaac aaggctgtg 
      aagagcaat agcggtgct tgcatgccg gtcctcgag 
      gacgagaag cagcaaaac ggaacggtt acgaagtcc 
      gacagtaac ggcactctt aacaacact tacatcaaa 
      agcgagtat tccgattcg tacaaagct tgctactcg 
      aacggcagc acaaacggc tttgtgagc caaaccgta 
      atagagcgc aactcgaag aagatcaac tag
    mRNA sequence
    • >TC011938 RA
      ccagatacc gaagttcgc tagtagaga gagattgag 
      tgtggttgg ttgtctcat gtctctctg acaccaacg 
      gcacgccta tatctcgca aaaatcgaa agaaagccc 
      accggaaaa gccccaaca atgtgttca aaccagtga 
      taaccgccc gtctcaaaa acagacttc aaaaaatcg 
      caactttac cggttttat tttgtgtga aaaacgtat 
      ccgtgtcgt gacaaacat tttagtggt gttttattg 
      attattttg ataaaggta acggcctca atacatact 
      ggacgtggt gctcatgtt aaaaggaaa gaaactagg 
      cgtacccat tgacctctc gctattttc tcgacaaag 
      aaaaattca acgatcgcg ggaacgtcc agtggaact 
      tttcctaat gccaatgca aggttgctc acatttttt 
      gcgattctg acgcatcac gaagattat cagataagg 
      ggttttgat gatgagcga tattagctg ccttatcat 
      tattaaaat aatttgtgc cgaattttc gacccagaa 
      aaacaggcg attgtttgg taatgaact tctttctat 
      gcagaaata ttttaattt taaaattca agccagtac 
      aagtggtga cgcgaacat ccgtcatct tgaattaat 
      tttttcgaa atcacgtgc cgtgcacaa ataaaatta 
      ttaattacc cgctgattg gcggaattt tatgaaatc 
      aaagtgcga ccgatcata acatccttc actgggaaa 
      acactattt ccttgcatt tgaggtgta ataacgtac 
      aaggataac aagctcgag acattcctc aagctaggc 
      agcatattt catcttcaa ttacaataa gttgattac 
      aaccagcgt aaagttgct acaaatgcc accataatt 
      tagccaaca tctgcatcg acagtaaaa cgagcaata 
      atcacgttt taccacgta tttagagtc aataacaat 
      aaccgtttt gtttggaat atttgggcg gactttgaa 
      taaattatt gtaattttc agactcaag atggctcat 
      ctcgtgacc agcgtcatc gacaaatac gaggacatt 
      ctagaaaac aaaagtgac ccgagggtc aaaggatgg 
      gccatgatg tctagtccc atgccaacg ctgtgcatt 
      tgcctattt tatgcgtac ttttcgacg gttctagga 
      ccccggatt atggaaaat aggaaaccg tttaattta 
      agaaagact ttaattttt tataattta tttcaaact 
      ctcttcagc gcgtggata ttttacgag tacttgatg 
      agcggctgg tgggggagc tacagcttc aggtgccaa 
      cctgtcgac tactcaaac agccccatg gcgctcagg 
      atggcgcgg acctgctgg tggtactac ttttcgaag 
      ttcaccgaa tttttcgac actctattt ttcattttg 
      cggaagaaa aatagccac gtgtccact ctccacgtc 
      atccaccac ggctgcatg ccgttttcg gtctggatg 
      gggatgaaa ttcgcgcca ggtggccac agcacgttc 
      ttcgccttg ctcaacaca ttcgtgcac atcatcatg 
      tatttctac tacatggtg gcggccatg gggccccag 
      taccagaaa tacatctgg tggaagaaa tacctgacc 
      acgttccag atggtgcag tttgtggcg attttcacc 
      caccagttc cagctgctc ttcaccgag tgcaattac 
      ccgaagagc ttcatgatt tggatcgct ttgcacggt 
      gtcatgttc ttattcctg ttctccgat ttttacaaa 
      gttaggtac accaacaac aaggctgtg aagagcaat 
      agcggtgct tgcatgccg gtcctcgag gacgagaag 
      cagcaaaac ggaacggtt acgaagtcc gacagtaac 
      ggcactctt aacaacact tacatcaaa agcgagtat 
      tccgattcg tacaaagct tgctactcg aacggcagc 
      acaaacggc tttgtgagc caaaccgta atagagcgc 
      aactcgaag aagatcaac taggtttta atttcagtg 
      ggtacaaaa ggactcgac ttttgttta attgtagac 
      ttatttatt agatagtgt acgatttgt tgtcaaaat 
      ttcgtatct cagggcgaa ataattggt ttcttttta 
      cttgatgta ttatataag tttttacgg ctgcaggga 
      ccattttca ttgcgtcaa gtcgcttga ttttttcat 
      ttcatcagc cattagtgc ctgaatttt taccacgtt 
      tcttagctt tagcttatt gttaaagtt ttgcacttt 
      gaatcgttg ccggttgtt cgtacttaa gaatgtttt 
      attacgttt tcgtataat ttatcggat gttttttat 
      tcagggagt tgcactctg ctgcgtaaa ttcggcggc 
      tctctacgt cttttcaga taagtaagt gtaatggaa 
      gctgtatca ctgtaatta atgtcgttt tattatcat 
      tgtatataa tacatacta cgcgtttct ttagattta 
      aagttgtat agtattttt gtatatact tatgaatct 
      tgagaaata aagtggaaa cctgcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY