Status
Please wait ...
Gene information
General information
TC011930 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11499920..11507247
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC011930
      iBeetle screen iB_05128

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011930 RA
        MGVHDLQVF LESPSLEGG AVSVDLLKI ARNVTQRQQ 
PHNTNRKIR PIGNKLKLI VDAENCLDR LYGGYFSDW 
ACGGQWNRM IQFLSLLTR AIERGNIEL AVVFNGTIE 
SCRMNEWVA EQANNRQKV GMVLKHINT KATPPPKIW 
WTPPTCLRS SLRMALRHL GVTVMCTMD DHHQEVIAY 
CREYGFHGL LADDAEYAA FDPPRYFSA ENLKLTYKG 
SLETKEFMI NELTKSLQL TQDQLCVMA ALMGNFLLT 
ESDLQDFHK KIGITNVIR DNKSPAEFN VKTLADFVR 
SLPPPSNMD ALVNAMLGY IDEKRASKF RQSVQYYLN 
GTASGFLKY CAPAATPKD KKNNAQNNN EKDNDDLEV 
SGTVFASET TEQEEETLH NYKLATANL VMSSEYTLD 
QNGTHNSNN DSSHFNGLL NGSVPNTNQ SLASTSSGS 
RVNLLVSSS SSNTSSVPS SPKHSAQST HSTAVLSNL 
VTPNVSPDV LRTASLRHQ KGLMASMIL HVLSTQEVR 
LPCLMEDEG NREFPPIHD VYQPLRQRV YAVLFNLHH 
LRYVHTKKK ESGEIPENS PQPDIVVKE WIYSRSNPY 
QCPEEVKAE PLPWAVPTL QRLWFGPAL DDKRRRMRA 
LLSCLNSDT PLILNTGYV PQHMLIMAC VLRYIMSYD 
KPIMRRHEL DAFLCQAFN VDLMNPQYL QELTLNVVT 
SRGVQLAAL FMQGVEMAL LANDACGAP LPWLMCCPW 
LYFDGKLFH YTLNRSAHS KNILEVCEN HIERVVKVE 
RMRKAILEG LDVKFAKMP LAPFPGMRQ PPPPQGLPT 
MPLPSNRIP GGMRPRPIS SRGGQLQVA GVVVGSWGA 
NYGYQQNIR QQAPLVGYQ GRGRGVMGP PGPSNFSQQ 
NIPRGRGQQ RKPGPPTFP INRKNNANK KRNNAGKGG 
SRAQGGQEP TLTVKTESG CVSRDIPAD EIILNNDGS 
LNTGGKSED CTKNSEHKG QGDAPNLVN
      CDS sequence
      • >TC011930 RA
        atgggggtc cacgatttg caagtattc ctcgaaagt 
cctagccta gaagggggc gccgtgtct gttgatttg 
ctaaaaatc gcccggaac gtaacgcag cgccaacag 
ccccacaat acgaaccgc aaaatccga ccaattggc 
aacaaactg aaactcatt gtggacgct gaaaactgc 
ctcgaccgg ctatatggg ggctatttt tcagattgg 
gcttgtggg ggacaatgg aaccgcatg atccagttc 
ctgtctcta ttaacacgt gcgatagaa cgtggtaac 
attgaattg gctgtggtg ttcaacggg acgatagag 
tcatgtcgg atgaacgaa tgggtggcc gaacaagcg 
aacaacagg caaaaagtc ggaatggtt ttgaaacac 
ataaatacc aaagctacg cctccgccg aaaatctgg 
tggacgccc ccgacctgt ctcaggtcc agcctcagg 
atggccctg aggcacctg ggagtgact gtgatgtgt 
accatggat gaccaccac caggaagtg atagcgtat 
tgccgggaa tatgggttc catggtctt ttggccgac 
gatgctgaa tatgctgcc ttcgaccca ccgcgatat 
ttttcagcc gaaaattta aaattaaca tataaaggc 
tctttggag accaaagaa ttcatgata aacgagctg 
accaagtcc cttcaattg acccaagac cagctttgc 
gtaatggca gctctaatg ggcaatttt ttgttaacc 
gaaagcgat ttacaagac tttcacaaa aaaatcggt 
atcacaaac gtaatccga gataacaaa tcaccagct 
gagtttaat gtaaaaacg ttggccgat ttcgttcgg 
tcgttacct ccaccttca aatatggac gctttagtt 
aatgctatg ctcggatat attgacgaa aaacgtgcc 
tcgaaattt agacagtcg gttcagtac tacctcaat 
ggtacggct agtggtttt cttaagtat tgtgcgccg 
gcagctaca cccaaagac aagaaaaat aatgcacaa 
aataacaat gagaaagat aatgatgat ttggaggtc 
tcggggacc gtatttgcc tctgaaact acagagcaa 
gaggaggaa actcttcac aattataaa ttagcaacc 
gcgaacttg gtcatgtcg tctgagtat acgctggat 
cagaacgga acacacaat tcaaataat gattcttct 
cattttaac ggactttta aatggttca gttcccaac 
actaatcag agtttggct agtacttct agcggcagt 
agagtcaac ctcctagtc agttcaagc agttcaaac 
accagcagt gttccatca tcgccgaaa cattcagct 
cagagtaca cattcaact gccgttttg tcgaatctg 
gtgacgcca aatgtttcg cccgacgtc ctccgaacg 
gcgtcgctg cgccaccaa aaaggctta atggcttcg 
atgattttg cacgttttg agtactcaa gaagtgcgt 
ctgccttgc ttgatggag gacgaaggc aatcgtgaa 
tttcctccg atccacgac gtgtaccag ccgctccga 
cagcgcgtc tatgcagta ttgttcaat ttacaccat 
ttgcgttac gttcacact aagaaaaaa gaatctggc 
gaaataccg gaaaattcg cctcaaccg gacatcgta 
gtcaaagaa tggatttac agcaggtcc aatccttac 
caatgccct gaagaagtc aaagctgaa cccttacca 
tgggcggtg cccacactt caaagattg tggttcggt 
ccggctctg gatgataaa agacgccga atgagggct 
ttgctgtct tgtctgaat tcagatacg cctttgata 
ctgaataca ggatacgtg cctcagcat atgttgata 
atggcgtgc gttttgcgc tatataatg tcgtatgat 
aagcctatt atgagacga cacgaattg gacgcgttt 
ctgtgtcag gctttcaat gttgatctc atgaatcct 
caatacttg caagaatta acactcaac gttgttact 
agtcgtggc gtacagttg gcagcgctg tttatgcaa 
ggtgttgag atggcactg ttggcgaac gatgcgtgt 
ggtgcccct ctaccgtgg ctcatgtgt tgtccttgg 
ttatacttt gacggcaaa ctgtttcat tatacgtta 
aacaggtcc gcacattcc aaaaatata cttgaagtg 
tgcgaaaat catatcgaa cgagttgtg aaagttgag 
aggatgagg aaggccatt ttggaaggg ttggatgtc 
aagtttgcc aaaatgcca ctagcacca tttccagga 
atgcgccag ccgccaccg cctcaaggg cttccaacc 
atgcccttg ccctcgaac cgcatccct ggcggaatg 
cgacctcgc cccatctca tctcgaggg gggcagttg 
caagttgcg ggtgttgtt gtgggttca tggggtgct 
aattacgga taccagcag aatataagg cagcaagcc 
ccattagtg ggttaccag gggcggggg cgcggcgtg 
atggggcct ccaggaccc agtaatttt agtcagcaa 
aatataccg aggggtagg ggccaacaa cgcaaacct 
ggaccaccg acattcccc atcaatagg aaaaataac 
gcaaataag aagcgtaac aatgcgggc aagggcggc 
agcagggca cagggaggt caggagccc acgcttaca 
gtaaaaact gaaagcggg tgcgtttca cgagacatt 
ccagctgac gaaataatc ctcaataac gatggttcg 
ctgaatact gggggaaaa tcggaagac tgcacgaag 
aacagcgag cataaaggt caaggcgat gccccgaat 
ctggttaat taa
      mRNA sequence
      • >TC011930 RA
        ttaaaacct ggcaaaaaa cgaagcttt ggcgctcgt 
tttcaagcg gtggttgca aaatggcgg gcgcgcgtt 
tccggaatg cgcagtaac ctctcgaag cgaagtttc 
cggtaaata ttttgttac tttactgcc gaaaaccat 
gaaatttta cgagttgat tattggtaa aaacgttca 
aactttgtt aaatgccaa catttcctt gcaactgta 
gtgtttttg ggttgattt ttggttttg gagcgcgca 
atacggtag aaaaaaaga agcaagcaa cgtgccggc 
atcttgaaa tgtgaaggc gtcgcgttg agtattatc 
agaaatggc catcagtgg taattaatt ttgatcgga 
acaactcgg aaatggggg tccacgatt tgcaagtat 
tcctcgaaa gtcctagcc tagaagggg gcgccgtgt 
ctgttgatt tgctaaaaa tcgcccgga acgtaacgc 
agcgccaac agccccaca atacgaacc gcaaaatcc 
gaccaattg gcaacaaac tgaaactca ttgtggacg 
ctgaaaact gcctcgacc ggctatatg ggggctatt 
tttcagatt gggcttgtg ggggacaat ggaaccgca 
tgatccagt tcctgtctc tattaacac gtgcgatag 
aacgtggta acattgaat tggctgtgg tgttcaacg 
ggacgatag agtcatgtc ggatgaacg aatgggtgg 
ccgaacaag cgaacaaca ggcaaaaag tcggaatgg 
ttttgaaac acataaata ccaaagcta cgcctccgc 
cgaaaatct ggtggacgc ccccgacct gtctcaggt 
ccagcctca ggatggccc tgaggcacc tgggagtga 
ctgtgatgt gtaccatgg atgaccacc accaggaag 
tgatagcgt attgccggg aatatgggt tccatggtc 
ttttggccg acgatgctg aatatgctg ccttcgacc 
caccgcgat atttttcag ccgaaaatt taaaattaa 
catataaag gctctttgg agaccaaag aattcatga 
taaacgagc tgaccaagt cccttcaat tgacccaag 
accagcttt gcgtaatgg cagctctaa tgggcaatt 
ttttgttaa ccgaaagcg atttacaag actttcaca 
aaaaaatcg gtatcacaa acgtaatcc gagataaca 
aatcaccag ctgagttta atgtaaaaa cgttggccg 
atttcgttc ggtcgttac ctccacctt caaatatgg 
acgctttag ttaatgcta tgctcggat atattgacg 
aaaaacgtg cctcgaaat ttagacagt cggttcagt 
actacctca atggtacgg ctagtggtt ttcttaagt 
attgtgcgc cggcagcta cacccaaag acaagaaaa 
ataatgcac aaaataaca atgagaaag ataatgatg 
atttggagg tctcgggga ccgtatttg cctctgaaa 
ctacagagc aagaggagg aaactcttc acaattata 
aattagcaa ccgcgaact tggtcatgt cgtctgagt 
atacgctgg atcagaacg gaacacaca attcaaata 
atgattctt ctcatttta acggacttt taaatggtt 
cagttccca acactaatc agagtttgg ctagtactt 
ctagcggca gtagagtca acctcctag tcagttcaa 
gcagttcaa acaccagca gtgttccat catcgccga 
aacattcag ctcagagta cacattcaa ctgccgttt 
tgtcgaatc tggtgacgc caaatgttt cgcccgacg 
tcctccgaa cggcgtcgc tgcgccacc aaaaaggct 
taatggctt cgatgattt tgcacgttt tgagtactc 
aagaagtgc gtctgcctt gcttgatgg aggacgaag 
gcaatcgtg aatttcctc cgatccacg acgtgtacc 
agccgctcc gacagcgcg tctatgcag tattgttca 
atttacacc atttgcgtt acgttcaca ctaagaaaa 
aagaatctg gcgaaatac cggaaaatt cgcctcaac 
cggacatcg tagtcaaag aatggattt acagcaggt 
ccaatcctt accaatgcc ctgaagaag tcaaagctg 
aacccttac catgggcgg tgcccacac ttcaaagat 
tgtggttcg gtccggctc tggatgata aaagacgcc 
gaatgaggg ctttgctgt cttgtctga attcagata 
cgcctttga tactgaata caggatacg tgcctcagc 
atatgttga taatggcgt gcgttttgc gctatataa 
tgtcgtatg ataagccta ttatgagac gacacgaat 
tggacgcgt ttctgtgtc aggctttca atgttgatc 
tcatgaatc ctcaatact tgcaagaat taacactca 
acgttgtta ctagtcgtg gcgtacagt tggcagcgc 
tgtttatgc aaggtgttg agatggcac tgttggcga 
acgatgcgt gtggtgccc ctctaccgt ggctcatgt 
gttgtcctt ggttatact ttgacggca aactgtttc 
attatacgt taaacaggt ccgcacatt ccaaaaata 
tacttgaag tgtgcgaaa atcatatcg aacgagttg 
tgaaagttg agaggatga ggaaggcca ttttggaag 
ggttggatg tcaagtttg ccaaaatgc cactagcac 
catttccag gaatgcgcc agccgccac cgcctcaag 
ggcttccaa ccatgccct tgccctcga accgcatcc 
ctggcggaa tgcgacctc gccccatct catctcgag 
gggggcagt tgcaagttg cgggtgttg ttgtgggtt 
catggggtg ctaattacg gataccagc agaatataa 
ggcagcaag ccccattag tgggttacc aggggcggg 
ggcgcggcg tgatggggc ctccaggac ccagtaatt 
ttagtcagc aaaatatac cgaggggta ggggccaac 
aacgcaaac ctggaccac cgacattcc ccatcaata 
ggaaaaata acgcaaata agaagcgta acaatgcgg 
gcaagggcg gcagcaggg cacagggag gtcaggagc 
ccacgctta cagtaaaaa ctgaaagcg ggtgcgttt 
cacgagaca ttccagctg acgaaataa tcctcaata 
acgatggtt cgctgaata ctgggggaa aatcggaag 
actgcacga agaacagcg agcataaag gtcaaggcg 
atgccccga atctggtta attaattct ggaagtgta 
cgtgttgtg tttgttttt ttatcgctg gattttttt 
tataatttt tttatgtat actttgttt acgaactcg 
gaagacttc tcaaaagtg gagaagtga agccttata 
tacagtcga ttaagtgtt aagagacac ggtgtggaa 
gactagggt gtgtgagtg tcttgtgtt atcataagt 
gactgactg gttttttaa atcgcgtta gctcaacaa 
cgataatct agcggtgac ggtacgtcg gatgaaaat 
taggcttgt aaggttggt agtaccggc gccgcttaa 
gctgcgttc agatgagag tctgaaacg ctgactgat 
tgtttgaga gcatatgta tatttttct atatttgtt 
acaactgtt ataattata tattgactg agagagtgc 
tttcagcac tgctgtgat catatttat ggtatttat 
acgtttaga tctgttgtt ggatcagca aaatgggtc 
gttgcgtgt gatagtcaa cgatttgtt ttgcttgaa 
gtgagagtt ggttttgga tggttccca gaaaacgtg 
acctttcgg tatatacca attaccagt tatttgcaa 
acgctctgt atttgaaaa ttgacatta aatcttgtc 
cattattct aagacaact ttaatatca tgcacagac 
ttcatgtaa gaaattcga tgttgaaac caaattact 
ttttcatcg aaacgaagt tttttgctg tttaactct 
gacttgcgc tattgctca tgagagaca atatagccg 
aaaattaac tgaacgcaa ctttgaatc caaatgatt 
cttaattta catcagaat attgtcaaa agtaaacaa 
acgaaacgc taaattgaa caatactta caattttac 
gtagctgtt tattgaact atacacatg tagagaaaa 
agaaacgat ttacataat ttagctcac caataatag 
acattgtat tagtaattt atccgttct ggatggaat 
tatcgtaat gttcatgaa atgtgtaaa cttttcgtt 
gtaaaattg ttacgcttc ctttgattt tgatggtgt 
attaatccc gccgttaca tttttctta ttaatatgt 
tatcgcttt tagttttgg gatacaaac attttttct 
ttatagtta ttttgttaa ttgatttac taattatgc 
aacgtttcc cttttttaa tcattatat gtatattct 
atgcttaaa ctatttaga tatttggaa agtatgtag 
agtatttac aattctttt ttgttctaa ttcacagta 
actgatata tttaacttt atatatgtg tatattggc 
tctatgggt gaaagggca tccatatga ataaatatt 
aaagtacct atatttaga taaaatagt atgtgattt 
tagaaaact tgggacaat atcacagaa ttctagatt 
gttttgttt cgttatcag gataaactt atcaatgtc 
tgaacctac cgcttattg tcttgatgt aactttctg 
aaatcacca taaattcaa agttttcac ttttttctt 
gtaatttat aattataaa aataaaaat cgcaagtcg 
atttgtttg taacgactt acgcaacac ttgactcat 
tacaaacaa ttgaactgc gatttgatg tggaagata 
tgctggaaa ggtaccacc aagtatctg taagatgca 
gatgaaaaa gtttgaatg a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY