| General information | |||
| TC011930 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 11499920..11507247 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC011930 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05128 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC011930 RA
MGVHDLQVF LESPSLEGG AVSVDLLKI ARNVTQRQQ PHNTNRKIR PIGNKLKLI VDAENCLDR LYGGYFSDW ACGGQWNRM IQFLSLLTR AIERGNIEL AVVFNGTIE SCRMNEWVA EQANNRQKV GMVLKHINT KATPPPKIW WTPPTCLRS SLRMALRHL GVTVMCTMD DHHQEVIAY CREYGFHGL LADDAEYAA FDPPRYFSA ENLKLTYKG SLETKEFMI NELTKSLQL TQDQLCVMA ALMGNFLLT ESDLQDFHK KIGITNVIR DNKSPAEFN VKTLADFVR SLPPPSNMD ALVNAMLGY IDEKRASKF RQSVQYYLN GTASGFLKY CAPAATPKD KKNNAQNNN EKDNDDLEV SGTVFASET TEQEEETLH NYKLATANL VMSSEYTLD QNGTHNSNN DSSHFNGLL NGSVPNTNQ SLASTSSGS RVNLLVSSS SSNTSSVPS SPKHSAQST HSTAVLSNL VTPNVSPDV LRTASLRHQ KGLMASMIL HVLSTQEVR LPCLMEDEG NREFPPIHD VYQPLRQRV YAVLFNLHH LRYVHTKKK ESGEIPENS PQPDIVVKE WIYSRSNPY QCPEEVKAE PLPWAVPTL QRLWFGPAL DDKRRRMRA LLSCLNSDT PLILNTGYV PQHMLIMAC VLRYIMSYD KPIMRRHEL DAFLCQAFN VDLMNPQYL QELTLNVVT SRGVQLAAL FMQGVEMAL LANDACGAP LPWLMCCPW LYFDGKLFH YTLNRSAHS KNILEVCEN HIERVVKVE RMRKAILEG LDVKFAKMP LAPFPGMRQ PPPPQGLPT MPLPSNRIP GGMRPRPIS SRGGQLQVA GVVVGSWGA NYGYQQNIR QQAPLVGYQ GRGRGVMGP PGPSNFSQQ NIPRGRGQQ RKPGPPTFP INRKNNANK KRNNAGKGG SRAQGGQEP TLTVKTESG CVSRDIPAD EIILNNDGS LNTGGKSED CTKNSEHKG QGDAPNLVN
- >TC011930 RA
atgggggtc cacgatttg caagtattc ctcgaaagt cctagccta gaagggggc gccgtgtct gttgatttg ctaaaaatc gcccggaac gtaacgcag cgccaacag ccccacaat acgaaccgc aaaatccga ccaattggc aacaaactg aaactcatt gtggacgct gaaaactgc ctcgaccgg ctatatggg ggctatttt tcagattgg gcttgtggg ggacaatgg aaccgcatg atccagttc ctgtctcta ttaacacgt gcgatagaa cgtggtaac attgaattg gctgtggtg ttcaacggg acgatagag tcatgtcgg atgaacgaa tgggtggcc gaacaagcg aacaacagg caaaaagtc ggaatggtt ttgaaacac ataaatacc aaagctacg cctccgccg aaaatctgg tggacgccc ccgacctgt ctcaggtcc agcctcagg atggccctg aggcacctg ggagtgact gtgatgtgt accatggat gaccaccac caggaagtg atagcgtat tgccgggaa tatgggttc catggtctt ttggccgac gatgctgaa tatgctgcc ttcgaccca ccgcgatat ttttcagcc gaaaattta aaattaaca tataaaggc tctttggag accaaagaa ttcatgata aacgagctg accaagtcc cttcaattg acccaagac cagctttgc gtaatggca gctctaatg ggcaatttt ttgttaacc gaaagcgat ttacaagac tttcacaaa aaaatcggt atcacaaac gtaatccga gataacaaa tcaccagct gagtttaat gtaaaaacg ttggccgat ttcgttcgg tcgttacct ccaccttca aatatggac gctttagtt aatgctatg ctcggatat attgacgaa aaacgtgcc tcgaaattt agacagtcg gttcagtac tacctcaat ggtacggct agtggtttt cttaagtat tgtgcgccg gcagctaca cccaaagac aagaaaaat aatgcacaa aataacaat gagaaagat aatgatgat ttggaggtc tcggggacc gtatttgcc tctgaaact acagagcaa gaggaggaa actcttcac aattataaa ttagcaacc gcgaacttg gtcatgtcg tctgagtat acgctggat cagaacgga acacacaat tcaaataat gattcttct cattttaac ggactttta aatggttca gttcccaac actaatcag agtttggct agtacttct agcggcagt agagtcaac ctcctagtc agttcaagc agttcaaac accagcagt gttccatca tcgccgaaa cattcagct cagagtaca cattcaact gccgttttg tcgaatctg gtgacgcca aatgtttcg cccgacgtc ctccgaacg gcgtcgctg cgccaccaa aaaggctta atggcttcg atgattttg cacgttttg agtactcaa gaagtgcgt ctgccttgc ttgatggag gacgaaggc aatcgtgaa tttcctccg atccacgac gtgtaccag ccgctccga cagcgcgtc tatgcagta ttgttcaat ttacaccat ttgcgttac gttcacact aagaaaaaa gaatctggc gaaataccg gaaaattcg cctcaaccg gacatcgta gtcaaagaa tggatttac agcaggtcc aatccttac caatgccct gaagaagtc aaagctgaa cccttacca tgggcggtg cccacactt caaagattg tggttcggt ccggctctg gatgataaa agacgccga atgagggct ttgctgtct tgtctgaat tcagatacg cctttgata ctgaataca ggatacgtg cctcagcat atgttgata atggcgtgc gttttgcgc tatataatg tcgtatgat aagcctatt atgagacga cacgaattg gacgcgttt ctgtgtcag gctttcaat gttgatctc atgaatcct caatacttg caagaatta acactcaac gttgttact agtcgtggc gtacagttg gcagcgctg tttatgcaa ggtgttgag atggcactg ttggcgaac gatgcgtgt ggtgcccct ctaccgtgg ctcatgtgt tgtccttgg ttatacttt gacggcaaa ctgtttcat tatacgtta aacaggtcc gcacattcc aaaaatata cttgaagtg tgcgaaaat catatcgaa cgagttgtg aaagttgag aggatgagg aaggccatt ttggaaggg ttggatgtc aagtttgcc aaaatgcca ctagcacca tttccagga atgcgccag ccgccaccg cctcaaggg cttccaacc atgcccttg ccctcgaac cgcatccct ggcggaatg cgacctcgc cccatctca tctcgaggg gggcagttg caagttgcg ggtgttgtt gtgggttca tggggtgct aattacgga taccagcag aatataagg cagcaagcc ccattagtg ggttaccag gggcggggg cgcggcgtg atggggcct ccaggaccc agtaatttt agtcagcaa aatataccg aggggtagg ggccaacaa cgcaaacct ggaccaccg acattcccc atcaatagg aaaaataac gcaaataag aagcgtaac aatgcgggc aagggcggc agcagggca cagggaggt caggagccc acgcttaca gtaaaaact gaaagcggg tgcgtttca cgagacatt ccagctgac gaaataatc ctcaataac gatggttcg ctgaatact gggggaaaa tcggaagac tgcacgaag aacagcgag cataaaggt caaggcgat gccccgaat ctggttaat taa
- >TC011930 RA
ttaaaacct ggcaaaaaa cgaagcttt ggcgctcgt tttcaagcg gtggttgca aaatggcgg gcgcgcgtt tccggaatg cgcagtaac ctctcgaag cgaagtttc cggtaaata ttttgttac tttactgcc gaaaaccat gaaatttta cgagttgat tattggtaa aaacgttca aactttgtt aaatgccaa catttcctt gcaactgta gtgtttttg ggttgattt ttggttttg gagcgcgca atacggtag aaaaaaaga agcaagcaa cgtgccggc atcttgaaa tgtgaaggc gtcgcgttg agtattatc agaaatggc catcagtgg taattaatt ttgatcgga acaactcgg aaatggggg tccacgatt tgcaagtat tcctcgaaa gtcctagcc tagaagggg gcgccgtgt ctgttgatt tgctaaaaa tcgcccgga acgtaacgc agcgccaac agccccaca atacgaacc gcaaaatcc gaccaattg gcaacaaac tgaaactca ttgtggacg ctgaaaact gcctcgacc ggctatatg ggggctatt tttcagatt gggcttgtg ggggacaat ggaaccgca tgatccagt tcctgtctc tattaacac gtgcgatag aacgtggta acattgaat tggctgtgg tgttcaacg ggacgatag agtcatgtc ggatgaacg aatgggtgg ccgaacaag cgaacaaca ggcaaaaag tcggaatgg ttttgaaac acataaata ccaaagcta cgcctccgc cgaaaatct ggtggacgc ccccgacct gtctcaggt ccagcctca ggatggccc tgaggcacc tgggagtga ctgtgatgt gtaccatgg atgaccacc accaggaag tgatagcgt attgccggg aatatgggt tccatggtc ttttggccg acgatgctg aatatgctg ccttcgacc caccgcgat atttttcag ccgaaaatt taaaattaa catataaag gctctttgg agaccaaag aattcatga taaacgagc tgaccaagt cccttcaat tgacccaag accagcttt gcgtaatgg cagctctaa tgggcaatt ttttgttaa ccgaaagcg atttacaag actttcaca aaaaaatcg gtatcacaa acgtaatcc gagataaca aatcaccag ctgagttta atgtaaaaa cgttggccg atttcgttc ggtcgttac ctccacctt caaatatgg acgctttag ttaatgcta tgctcggat atattgacg aaaaacgtg cctcgaaat ttagacagt cggttcagt actacctca atggtacgg ctagtggtt ttcttaagt attgtgcgc cggcagcta cacccaaag acaagaaaa ataatgcac aaaataaca atgagaaag ataatgatg atttggagg tctcgggga ccgtatttg cctctgaaa ctacagagc aagaggagg aaactcttc acaattata aattagcaa ccgcgaact tggtcatgt cgtctgagt atacgctgg atcagaacg gaacacaca attcaaata atgattctt ctcatttta acggacttt taaatggtt cagttccca acactaatc agagtttgg ctagtactt ctagcggca gtagagtca acctcctag tcagttcaa gcagttcaa acaccagca gtgttccat catcgccga aacattcag ctcagagta cacattcaa ctgccgttt tgtcgaatc tggtgacgc caaatgttt cgcccgacg tcctccgaa cggcgtcgc tgcgccacc aaaaaggct taatggctt cgatgattt tgcacgttt tgagtactc aagaagtgc gtctgcctt gcttgatgg aggacgaag gcaatcgtg aatttcctc cgatccacg acgtgtacc agccgctcc gacagcgcg tctatgcag tattgttca atttacacc atttgcgtt acgttcaca ctaagaaaa aagaatctg gcgaaatac cggaaaatt cgcctcaac cggacatcg tagtcaaag aatggattt acagcaggt ccaatcctt accaatgcc ctgaagaag tcaaagctg aacccttac catgggcgg tgcccacac ttcaaagat tgtggttcg gtccggctc tggatgata aaagacgcc gaatgaggg ctttgctgt cttgtctga attcagata cgcctttga tactgaata caggatacg tgcctcagc atatgttga taatggcgt gcgttttgc gctatataa tgtcgtatg ataagccta ttatgagac gacacgaat tggacgcgt ttctgtgtc aggctttca atgttgatc tcatgaatc ctcaatact tgcaagaat taacactca acgttgtta ctagtcgtg gcgtacagt tggcagcgc tgtttatgc aaggtgttg agatggcac tgttggcga acgatgcgt gtggtgccc ctctaccgt ggctcatgt gttgtcctt ggttatact ttgacggca aactgtttc attatacgt taaacaggt ccgcacatt ccaaaaata tacttgaag tgtgcgaaa atcatatcg aacgagttg tgaaagttg agaggatga ggaaggcca ttttggaag ggttggatg tcaagtttg ccaaaatgc cactagcac catttccag gaatgcgcc agccgccac cgcctcaag ggcttccaa ccatgccct tgccctcga accgcatcc ctggcggaa tgcgacctc gccccatct catctcgag gggggcagt tgcaagttg cgggtgttg ttgtgggtt catggggtg ctaattacg gataccagc agaatataa ggcagcaag ccccattag tgggttacc aggggcggg ggcgcggcg tgatggggc ctccaggac ccagtaatt ttagtcagc aaaatatac cgaggggta ggggccaac aacgcaaac ctggaccac cgacattcc ccatcaata ggaaaaata acgcaaata agaagcgta acaatgcgg gcaagggcg gcagcaggg cacagggag gtcaggagc ccacgctta cagtaaaaa ctgaaagcg ggtgcgttt cacgagaca ttccagctg acgaaataa tcctcaata acgatggtt cgctgaata ctgggggaa aatcggaag actgcacga agaacagcg agcataaag gtcaaggcg atgccccga atctggtta attaattct ggaagtgta cgtgttgtg tttgttttt ttatcgctg gattttttt tataatttt tttatgtat actttgttt acgaactcg gaagacttc tcaaaagtg gagaagtga agccttata tacagtcga ttaagtgtt aagagacac ggtgtggaa gactagggt gtgtgagtg tcttgtgtt atcataagt gactgactg gttttttaa atcgcgtta gctcaacaa cgataatct agcggtgac ggtacgtcg gatgaaaat taggcttgt aaggttggt agtaccggc gccgcttaa gctgcgttc agatgagag tctgaaacg ctgactgat tgtttgaga gcatatgta tatttttct atatttgtt acaactgtt ataattata tattgactg agagagtgc tttcagcac tgctgtgat catatttat ggtatttat acgtttaga tctgttgtt ggatcagca aaatgggtc gttgcgtgt gatagtcaa cgatttgtt ttgcttgaa gtgagagtt ggttttgga tggttccca gaaaacgtg acctttcgg tatatacca attaccagt tatttgcaa acgctctgt atttgaaaa ttgacatta aatcttgtc cattattct aagacaact ttaatatca tgcacagac ttcatgtaa gaaattcga tgttgaaac caaattact ttttcatcg aaacgaagt tttttgctg tttaactct gacttgcgc tattgctca tgagagaca atatagccg aaaattaac tgaacgcaa ctttgaatc caaatgatt cttaattta catcagaat attgtcaaa agtaaacaa acgaaacgc taaattgaa caatactta caattttac gtagctgtt tattgaact atacacatg tagagaaaa agaaacgat ttacataat ttagctcac caataatag acattgtat tagtaattt atccgttct ggatggaat tatcgtaat gttcatgaa atgtgtaaa cttttcgtt gtaaaattg ttacgcttc ctttgattt tgatggtgt attaatccc gccgttaca tttttctta ttaatatgt tatcgcttt tagttttgg gatacaaac attttttct ttatagtta ttttgttaa ttgatttac taattatgc aacgtttcc cttttttaa tcattatat gtatattct atgcttaaa ctatttaga tatttggaa agtatgtag agtatttac aattctttt ttgttctaa ttcacagta actgatata tttaacttt atatatgtg tatattggc tctatgggt gaaagggca tccatatga ataaatatt aaagtacct atatttaga taaaatagt atgtgattt tagaaaact tgggacaat atcacagaa ttctagatt gttttgttt cgttatcag gataaactt atcaatgtc tgaacctac cgcttattg tcttgatgt aactttctg aaatcacca taaattcaa agttttcac ttttttctt gtaatttat aattataaa aataaaaat cgcaagtcg atttgtttg taacgactt acgcaacac ttgactcat tacaaacaa ttgaactgc gatttgatg tggaagata tgctggaaa ggtaccacc aagtatctg taagatgca gatgaaaaa gtttgaatg a
