Status
Please wait ...
Gene information
General information
TC011930 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11499920..11507247
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011930
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05128

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011930 RA
        MGVHDLQVF LESPSLEGG AVSVDLLKI ARNVTQRQQ 
        PHNTNRKIR PIGNKLKLI VDAENCLDR LYGGYFSDW 
        ACGGQWNRM IQFLSLLTR AIERGNIEL AVVFNGTIE 
        SCRMNEWVA EQANNRQKV GMVLKHINT KATPPPKIW 
        WTPPTCLRS SLRMALRHL GVTVMCTMD DHHQEVIAY 
        CREYGFHGL LADDAEYAA FDPPRYFSA ENLKLTYKG 
        SLETKEFMI NELTKSLQL TQDQLCVMA ALMGNFLLT 
        ESDLQDFHK KIGITNVIR DNKSPAEFN VKTLADFVR 
        SLPPPSNMD ALVNAMLGY IDEKRASKF RQSVQYYLN 
        GTASGFLKY CAPAATPKD KKNNAQNNN EKDNDDLEV 
        SGTVFASET TEQEEETLH NYKLATANL VMSSEYTLD 
        QNGTHNSNN DSSHFNGLL NGSVPNTNQ SLASTSSGS 
        RVNLLVSSS SSNTSSVPS SPKHSAQST HSTAVLSNL 
        VTPNVSPDV LRTASLRHQ KGLMASMIL HVLSTQEVR 
        LPCLMEDEG NREFPPIHD VYQPLRQRV YAVLFNLHH 
        LRYVHTKKK ESGEIPENS PQPDIVVKE WIYSRSNPY 
        QCPEEVKAE PLPWAVPTL QRLWFGPAL DDKRRRMRA 
        LLSCLNSDT PLILNTGYV PQHMLIMAC VLRYIMSYD 
        KPIMRRHEL DAFLCQAFN VDLMNPQYL QELTLNVVT 
        SRGVQLAAL FMQGVEMAL LANDACGAP LPWLMCCPW 
        LYFDGKLFH YTLNRSAHS KNILEVCEN HIERVVKVE 
        RMRKAILEG LDVKFAKMP LAPFPGMRQ PPPPQGLPT 
        MPLPSNRIP GGMRPRPIS SRGGQLQVA GVVVGSWGA 
        NYGYQQNIR QQAPLVGYQ GRGRGVMGP PGPSNFSQQ 
        NIPRGRGQQ RKPGPPTFP INRKNNANK KRNNAGKGG 
        SRAQGGQEP TLTVKTESG CVSRDIPAD EIILNNDGS 
        LNTGGKSED CTKNSEHKG QGDAPNLVN
      CDS sequence
      • >TC011930 RA
        atgggggtc cacgatttg caagtattc ctcgaaagt 
        cctagccta gaagggggc gccgtgtct gttgatttg 
        ctaaaaatc gcccggaac gtaacgcag cgccaacag 
        ccccacaat acgaaccgc aaaatccga ccaattggc 
        aacaaactg aaactcatt gtggacgct gaaaactgc 
        ctcgaccgg ctatatggg ggctatttt tcagattgg 
        gcttgtggg ggacaatgg aaccgcatg atccagttc 
        ctgtctcta ttaacacgt gcgatagaa cgtggtaac 
        attgaattg gctgtggtg ttcaacggg acgatagag 
        tcatgtcgg atgaacgaa tgggtggcc gaacaagcg 
        aacaacagg caaaaagtc ggaatggtt ttgaaacac 
        ataaatacc aaagctacg cctccgccg aaaatctgg 
        tggacgccc ccgacctgt ctcaggtcc agcctcagg 
        atggccctg aggcacctg ggagtgact gtgatgtgt 
        accatggat gaccaccac caggaagtg atagcgtat 
        tgccgggaa tatgggttc catggtctt ttggccgac 
        gatgctgaa tatgctgcc ttcgaccca ccgcgatat 
        ttttcagcc gaaaattta aaattaaca tataaaggc 
        tctttggag accaaagaa ttcatgata aacgagctg 
        accaagtcc cttcaattg acccaagac cagctttgc 
        gtaatggca gctctaatg ggcaatttt ttgttaacc 
        gaaagcgat ttacaagac tttcacaaa aaaatcggt 
        atcacaaac gtaatccga gataacaaa tcaccagct 
        gagtttaat gtaaaaacg ttggccgat ttcgttcgg 
        tcgttacct ccaccttca aatatggac gctttagtt 
        aatgctatg ctcggatat attgacgaa aaacgtgcc 
        tcgaaattt agacagtcg gttcagtac tacctcaat 
        ggtacggct agtggtttt cttaagtat tgtgcgccg 
        gcagctaca cccaaagac aagaaaaat aatgcacaa 
        aataacaat gagaaagat aatgatgat ttggaggtc 
        tcggggacc gtatttgcc tctgaaact acagagcaa 
        gaggaggaa actcttcac aattataaa ttagcaacc 
        gcgaacttg gtcatgtcg tctgagtat acgctggat 
        cagaacgga acacacaat tcaaataat gattcttct 
        cattttaac ggactttta aatggttca gttcccaac 
        actaatcag agtttggct agtacttct agcggcagt 
        agagtcaac ctcctagtc agttcaagc agttcaaac 
        accagcagt gttccatca tcgccgaaa cattcagct 
        cagagtaca cattcaact gccgttttg tcgaatctg 
        gtgacgcca aatgtttcg cccgacgtc ctccgaacg 
        gcgtcgctg cgccaccaa aaaggctta atggcttcg 
        atgattttg cacgttttg agtactcaa gaagtgcgt 
        ctgccttgc ttgatggag gacgaaggc aatcgtgaa 
        tttcctccg atccacgac gtgtaccag ccgctccga 
        cagcgcgtc tatgcagta ttgttcaat ttacaccat 
        ttgcgttac gttcacact aagaaaaaa gaatctggc 
        gaaataccg gaaaattcg cctcaaccg gacatcgta 
        gtcaaagaa tggatttac agcaggtcc aatccttac 
        caatgccct gaagaagtc aaagctgaa cccttacca 
        tgggcggtg cccacactt caaagattg tggttcggt 
        ccggctctg gatgataaa agacgccga atgagggct 
        ttgctgtct tgtctgaat tcagatacg cctttgata 
        ctgaataca ggatacgtg cctcagcat atgttgata 
        atggcgtgc gttttgcgc tatataatg tcgtatgat 
        aagcctatt atgagacga cacgaattg gacgcgttt 
        ctgtgtcag gctttcaat gttgatctc atgaatcct 
        caatacttg caagaatta acactcaac gttgttact 
        agtcgtggc gtacagttg gcagcgctg tttatgcaa 
        ggtgttgag atggcactg ttggcgaac gatgcgtgt 
        ggtgcccct ctaccgtgg ctcatgtgt tgtccttgg 
        ttatacttt gacggcaaa ctgtttcat tatacgtta 
        aacaggtcc gcacattcc aaaaatata cttgaagtg 
        tgcgaaaat catatcgaa cgagttgtg aaagttgag 
        aggatgagg aaggccatt ttggaaggg ttggatgtc 
        aagtttgcc aaaatgcca ctagcacca tttccagga 
        atgcgccag ccgccaccg cctcaaggg cttccaacc 
        atgcccttg ccctcgaac cgcatccct ggcggaatg 
        cgacctcgc cccatctca tctcgaggg gggcagttg 
        caagttgcg ggtgttgtt gtgggttca tggggtgct 
        aattacgga taccagcag aatataagg cagcaagcc 
        ccattagtg ggttaccag gggcggggg cgcggcgtg 
        atggggcct ccaggaccc agtaatttt agtcagcaa 
        aatataccg aggggtagg ggccaacaa cgcaaacct 
        ggaccaccg acattcccc atcaatagg aaaaataac 
        gcaaataag aagcgtaac aatgcgggc aagggcggc 
        agcagggca cagggaggt caggagccc acgcttaca 
        gtaaaaact gaaagcggg tgcgtttca cgagacatt 
        ccagctgac gaaataatc ctcaataac gatggttcg 
        ctgaatact gggggaaaa tcggaagac tgcacgaag 
        aacagcgag cataaaggt caaggcgat gccccgaat 
        ctggttaat taa
      mRNA sequence
      • >TC011930 RA
        ttaaaacct ggcaaaaaa cgaagcttt ggcgctcgt 
        tttcaagcg gtggttgca aaatggcgg gcgcgcgtt 
        tccggaatg cgcagtaac ctctcgaag cgaagtttc 
        cggtaaata ttttgttac tttactgcc gaaaaccat 
        gaaatttta cgagttgat tattggtaa aaacgttca 
        aactttgtt aaatgccaa catttcctt gcaactgta 
        gtgtttttg ggttgattt ttggttttg gagcgcgca 
        atacggtag aaaaaaaga agcaagcaa cgtgccggc 
        atcttgaaa tgtgaaggc gtcgcgttg agtattatc 
        agaaatggc catcagtgg taattaatt ttgatcgga 
        acaactcgg aaatggggg tccacgatt tgcaagtat 
        tcctcgaaa gtcctagcc tagaagggg gcgccgtgt 
        ctgttgatt tgctaaaaa tcgcccgga acgtaacgc 
        agcgccaac agccccaca atacgaacc gcaaaatcc 
        gaccaattg gcaacaaac tgaaactca ttgtggacg 
        ctgaaaact gcctcgacc ggctatatg ggggctatt 
        tttcagatt gggcttgtg ggggacaat ggaaccgca 
        tgatccagt tcctgtctc tattaacac gtgcgatag 
        aacgtggta acattgaat tggctgtgg tgttcaacg 
        ggacgatag agtcatgtc ggatgaacg aatgggtgg 
        ccgaacaag cgaacaaca ggcaaaaag tcggaatgg 
        ttttgaaac acataaata ccaaagcta cgcctccgc 
        cgaaaatct ggtggacgc ccccgacct gtctcaggt 
        ccagcctca ggatggccc tgaggcacc tgggagtga 
        ctgtgatgt gtaccatgg atgaccacc accaggaag 
        tgatagcgt attgccggg aatatgggt tccatggtc 
        ttttggccg acgatgctg aatatgctg ccttcgacc 
        caccgcgat atttttcag ccgaaaatt taaaattaa 
        catataaag gctctttgg agaccaaag aattcatga 
        taaacgagc tgaccaagt cccttcaat tgacccaag 
        accagcttt gcgtaatgg cagctctaa tgggcaatt 
        ttttgttaa ccgaaagcg atttacaag actttcaca 
        aaaaaatcg gtatcacaa acgtaatcc gagataaca 
        aatcaccag ctgagttta atgtaaaaa cgttggccg 
        atttcgttc ggtcgttac ctccacctt caaatatgg 
        acgctttag ttaatgcta tgctcggat atattgacg 
        aaaaacgtg cctcgaaat ttagacagt cggttcagt 
        actacctca atggtacgg ctagtggtt ttcttaagt 
        attgtgcgc cggcagcta cacccaaag acaagaaaa 
        ataatgcac aaaataaca atgagaaag ataatgatg 
        atttggagg tctcgggga ccgtatttg cctctgaaa 
        ctacagagc aagaggagg aaactcttc acaattata 
        aattagcaa ccgcgaact tggtcatgt cgtctgagt 
        atacgctgg atcagaacg gaacacaca attcaaata 
        atgattctt ctcatttta acggacttt taaatggtt 
        cagttccca acactaatc agagtttgg ctagtactt 
        ctagcggca gtagagtca acctcctag tcagttcaa 
        gcagttcaa acaccagca gtgttccat catcgccga 
        aacattcag ctcagagta cacattcaa ctgccgttt 
        tgtcgaatc tggtgacgc caaatgttt cgcccgacg 
        tcctccgaa cggcgtcgc tgcgccacc aaaaaggct 
        taatggctt cgatgattt tgcacgttt tgagtactc 
        aagaagtgc gtctgcctt gcttgatgg aggacgaag 
        gcaatcgtg aatttcctc cgatccacg acgtgtacc 
        agccgctcc gacagcgcg tctatgcag tattgttca 
        atttacacc atttgcgtt acgttcaca ctaagaaaa 
        aagaatctg gcgaaatac cggaaaatt cgcctcaac 
        cggacatcg tagtcaaag aatggattt acagcaggt 
        ccaatcctt accaatgcc ctgaagaag tcaaagctg 
        aacccttac catgggcgg tgcccacac ttcaaagat 
        tgtggttcg gtccggctc tggatgata aaagacgcc 
        gaatgaggg ctttgctgt cttgtctga attcagata 
        cgcctttga tactgaata caggatacg tgcctcagc 
        atatgttga taatggcgt gcgttttgc gctatataa 
        tgtcgtatg ataagccta ttatgagac gacacgaat 
        tggacgcgt ttctgtgtc aggctttca atgttgatc 
        tcatgaatc ctcaatact tgcaagaat taacactca 
        acgttgtta ctagtcgtg gcgtacagt tggcagcgc 
        tgtttatgc aaggtgttg agatggcac tgttggcga 
        acgatgcgt gtggtgccc ctctaccgt ggctcatgt 
        gttgtcctt ggttatact ttgacggca aactgtttc 
        attatacgt taaacaggt ccgcacatt ccaaaaata 
        tacttgaag tgtgcgaaa atcatatcg aacgagttg 
        tgaaagttg agaggatga ggaaggcca ttttggaag 
        ggttggatg tcaagtttg ccaaaatgc cactagcac 
        catttccag gaatgcgcc agccgccac cgcctcaag 
        ggcttccaa ccatgccct tgccctcga accgcatcc 
        ctggcggaa tgcgacctc gccccatct catctcgag 
        gggggcagt tgcaagttg cgggtgttg ttgtgggtt 
        catggggtg ctaattacg gataccagc agaatataa 
        ggcagcaag ccccattag tgggttacc aggggcggg 
        ggcgcggcg tgatggggc ctccaggac ccagtaatt 
        ttagtcagc aaaatatac cgaggggta ggggccaac 
        aacgcaaac ctggaccac cgacattcc ccatcaata 
        ggaaaaata acgcaaata agaagcgta acaatgcgg 
        gcaagggcg gcagcaggg cacagggag gtcaggagc 
        ccacgctta cagtaaaaa ctgaaagcg ggtgcgttt 
        cacgagaca ttccagctg acgaaataa tcctcaata 
        acgatggtt cgctgaata ctgggggaa aatcggaag 
        actgcacga agaacagcg agcataaag gtcaaggcg 
        atgccccga atctggtta attaattct ggaagtgta 
        cgtgttgtg tttgttttt ttatcgctg gattttttt 
        tataatttt tttatgtat actttgttt acgaactcg 
        gaagacttc tcaaaagtg gagaagtga agccttata 
        tacagtcga ttaagtgtt aagagacac ggtgtggaa 
        gactagggt gtgtgagtg tcttgtgtt atcataagt 
        gactgactg gttttttaa atcgcgtta gctcaacaa 
        cgataatct agcggtgac ggtacgtcg gatgaaaat 
        taggcttgt aaggttggt agtaccggc gccgcttaa 
        gctgcgttc agatgagag tctgaaacg ctgactgat 
        tgtttgaga gcatatgta tatttttct atatttgtt 
        acaactgtt ataattata tattgactg agagagtgc 
        tttcagcac tgctgtgat catatttat ggtatttat 
        acgtttaga tctgttgtt ggatcagca aaatgggtc 
        gttgcgtgt gatagtcaa cgatttgtt ttgcttgaa 
        gtgagagtt ggttttgga tggttccca gaaaacgtg 
        acctttcgg tatatacca attaccagt tatttgcaa 
        acgctctgt atttgaaaa ttgacatta aatcttgtc 
        cattattct aagacaact ttaatatca tgcacagac 
        ttcatgtaa gaaattcga tgttgaaac caaattact 
        ttttcatcg aaacgaagt tttttgctg tttaactct 
        gacttgcgc tattgctca tgagagaca atatagccg 
        aaaattaac tgaacgcaa ctttgaatc caaatgatt 
        cttaattta catcagaat attgtcaaa agtaaacaa 
        acgaaacgc taaattgaa caatactta caattttac 
        gtagctgtt tattgaact atacacatg tagagaaaa 
        agaaacgat ttacataat ttagctcac caataatag 
        acattgtat tagtaattt atccgttct ggatggaat 
        tatcgtaat gttcatgaa atgtgtaaa cttttcgtt 
        gtaaaattg ttacgcttc ctttgattt tgatggtgt 
        attaatccc gccgttaca tttttctta ttaatatgt 
        tatcgcttt tagttttgg gatacaaac attttttct 
        ttatagtta ttttgttaa ttgatttac taattatgc 
        aacgtttcc cttttttaa tcattatat gtatattct 
        atgcttaaa ctatttaga tatttggaa agtatgtag 
        agtatttac aattctttt ttgttctaa ttcacagta 
        actgatata tttaacttt atatatgtg tatattggc 
        tctatgggt gaaagggca tccatatga ataaatatt 
        aaagtacct atatttaga taaaatagt atgtgattt 
        tagaaaact tgggacaat atcacagaa ttctagatt 
        gttttgttt cgttatcag gataaactt atcaatgtc 
        tgaacctac cgcttattg tcttgatgt aactttctg 
        aaatcacca taaattcaa agttttcac ttttttctt 
        gtaatttat aattataaa aataaaaat cgcaagtcg 
        atttgtttg taacgactt acgcaacac ttgactcat 
        tacaaacaa ttgaactgc gatttgatg tggaagata 
        tgctggaaa ggtaccacc aagtatctg taagatgca 
        gatgaaaaa gtttgaatg a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY