Status
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Gene information
General information
TC011927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11559790..11563176
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011927
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05127

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011927 RA
      MTTCSEYIK NVFPLIDDD LKHYVEGVL QNGADDFQD 
      SEEVYEAIG EVLQEVSSD KTEEDIRNI CNELHNMMK 
      PDKSNAKNG PTKVLNAPV HLASMAANL ENNIGDIKS 
      IWVMQRDDT LKVDAKKLE KAEAKLQEK QEKRSKDVK 
      TAAPVKLES ATASQVTSK KESKMEAKG NNRTQDIRI 
      ENFDVAYGD RVLLQGADL ILAFGRRYG LVGRNGLGK 
      STLLRMISN GQLRIPSHI SILHVEQEV IGDDTLAIQ 
      SVLECDTVR ESLLKREKE ISAAVNSGN SDPELSNQL 
      TEIYAQLQN IEADKAPAK ASIILNGLG FTPEMQQRE 
      TKTFSGGWR MRLALARAL FSRPDLLLL DEPTNMLDI 
      KAIIWLENY LQNWPTTLL VVSHDRNFL DTVPTDILH 
      LHSQRIDAY RGNYEQFEK TKTEKLKNQ QREYEAQMQ 
      HRQHVQVFI DKFRYNANR AASVQSKIK LLDKLPELK 
      PIEKETAVV LKFPDTEPL SPPILQLNE VSFGYNKDR 
      LIFTNVNLG ATMDSRICI VGDNGAGKT TLLKIIMGL 
      LSPSAGMLH VHRNLKFGY FSQHHVDQL DMNVNSVEL 
      LQSTYPGKP IEEYRRQLG SFGVSGDLA LQTVASLSG 
      GQKSRVAFA RMCMGNPNF LVLDEPTNH LDIETIEAL 
      GNAIVKFTG GVILVSHDE RLIRKVCKE LWVCGNGSV 
      RSIEGFDEY RRLVEQELE SQSK
    CDS sequence
    • >TC011927 RA
      atgaccact tgtagtgaa tacattaaa aacgtgttc 
      cctttaatt gacgacgac ctcaagcat tacgttgaa 
      ggagtctta caaaacggt gctgatgat ttccaagac 
      agcgaagag gtgtacgaa gccatcggt gaggtgtta 
      caggaagtt tccagtgac aaaaccgaa gaagacata 
      cggaacata tgcaacgag ttgcacaat atgatgaaa 
      cctgacaaa agcaatgcc aaaaacggc cccacaaag 
      gtgctaaac gcaccggta cacctggct tcaatggcc 
      gcaaacttg gaaaataac atcggtgat atcaaaagt 
      atttgggtt atgcagcgt gacgacacc ttgaaagtc 
      gatgctaaa aaactcgaa aaagccgag gccaaattg 
      caggaaaag caagaaaag cgctccaaa gacgtcaaa 
      accgcagcc cctgttaaa ctagagtcc gcgacggct 
      tctcaagtc accagcaaa aaagagagc aaaatggag 
      gccaaaggc aataatagg actcaggac atacggatc 
      gaaaacttc gatgtggct tacggcgac cgggttctc 
      ctgcaaggc gccgacttg atcctggcc ttcggccgg 
      cgctacggc ctcgtgggg cgcaacggc ttgggaaaa 
      tcaaccctc ctgaggatg atttcaaac ggccaactg 
      cgcatcccg tcccacatt tcgattttg cacgtggaa 
      caagaggtc ataggtgac gacactttg gccatccag 
      agcgtgctt gagtgcgat acggtccgg gaaagttta 
      ctcaaacgc gagaaggaa ataagtgcc gcggtcaac 
      tcgggaaat agcgacccg gaattgtcg aatcagctg 
      accgagatc tacgcccaa cttcagaat atcgaagct 
      gataaagcc ccagctaag gcgtcgatt attttgaac 
      gggttgggg ttcactcct gagatgcag cagagggag 
      actaagacg ttttctggc ggctggagg atgaggctt 
      gctttggcc agggcgttg ttttcgcgc cccgatttg 
      ctgcttttg gacgaaccg acgaacatg ttggatatt 
      aaggcgatt atttggctg gagaattat ttacagaat 
      tggccgaca acgttacta gtggtttcc cacgatcga 
      aattttcta gatacggtc ccgacggat attttgcac 
      ttgcattcg caaaggata gatgcctac aggggaaat 
      tatgaacag tttgagaaa actaaaacc gagaaacta 
      aaaaatcag cagagagag tacgaggcg caaatgcag 
      cacagacaa cacgtccaa gtgttcatt gataagttt 
      aggtacaat gccaacaga gcggcctca gtgcagtca 
      aaaatcaaa ttgttggat aaattacct gaattgaag 
      ccaatagaa aaggaaact gctgtagtt ttgaaattc 
      ccagatacc gaacctctc tcacccccc attttgcaa 
      cttaacgaa gtttcgttt ggttacaac aaagataga 
      ctcatattt accaacgtt aatctaggg gctaccatg 
      gattccagg atatgtatt gtcggtgac aacggtgct 
      ggtaaaacc accctcctc aaaatcatc atgggcctc 
      ctgtcccca tctgcgggc atgctccac gtccatcga 
      aacctaaaa ttcggctac ttcagccag caccacgtg 
      gaccaactc gacatgaac gtcaactcg gtcgagctc 
      ctccaaagc acatatccc ggaaaacca atcgaggaa 
      tatagacgc cagcttggg agttttggc gtctcgggc 
      gacctcgcc ctccaaacc gtcgccagt ttatcaggg 
      gggcaaaaa tctcgagtc gcttttgcc cgaatgtgc 
      atgggtaac ccaaatttc ctcgtactt gacgaaccc 
      accaatcat ttagatatc gaaacaatt gaagcttta 
      gggaatgcg atagtcaaa ttcaccggc ggtgtgata 
      cttgtgtcg cacgacgag cggcttatt cgcaaagtg 
      tgcaaggag ttgtgggtt tgtggtaat ggctctgtg 
      cgaagtata gaaggtttt gatgagtac agacgactt 
      gtcgaacag gaactcgaa agtcagtct aaataa
    mRNA sequence
    • >TC011927 RA
      tgctggttg gcagcgcat ttttccttt gtcagcagt 
      gtcagcacg tcaaaaaat atttagagg ttaaaacgt 
      tttttaaat tgctcattt ttagccaaa tttgccaaa 
      acctagtaa aagtccacc atgaccact tgtagtgaa 
      tacattaaa aacgtgttc cctttaatt gacgacgac 
      ctcaagcat tacgttgaa ggagtctta caaaacggt 
      gctgatgat ttccaagac agcgaagag gtgtacgaa 
      gccatcggt gaggtgtta caggaagtt tccagtgac 
      aaaaccgaa gaagacata cggaacata tgcaacgag 
      ttgcacaat atgatgaaa cctgacaaa agcaatgcc 
      aaaaacggc cccacaaag gtgctaaac gcaccggta 
      cacctggct tcaatggcc gcaaacttg gaaaataac 
      atcggtgat atcaaaagt atttgggtt atgcagcgt 
      gacgacacc ttgaaagtc gatgctaaa aaactcgaa 
      aaagccgag gccaaattg caggaaaag caagaaaag 
      cgctccaaa gacgtcaaa accgcagcc cctgttaaa 
      ctagagtcc gcgacggct tctcaagtc accagcaaa 
      aaagagagc aaaatggag gccaaaggc aataatagg 
      actcaggac atacggatc gaaaacttc gatgtggct 
      tacggcgac cgggttctc ctgcaaggc gccgacttg 
      atcctggcc ttcggccgg cgctacggc ctcgtgggg 
      cgcaacggc ttgggaaaa tcaaccctc ctgaggatg 
      atttcaaac ggccaactg cgcatcccg tcccacatt 
      tcgattttg cacgtggaa caagaggtc ataggtgac 
      gacactttg gccatccag agcgtgctt gagtgcgat 
      acggtccgg gaaagttta ctcaaacgc gagaaggaa 
      ataagtgcc gcggtcaac tcgggaaat agcgacccg 
      gaattgtcg aatcagctg accgagatc tacgcccaa 
      cttcagaat atcgaagct gataaagcc ccagctaag 
      gcgtcgatt attttgaac gggttgggg ttcactcct 
      gagatgcag cagagggag actaagacg ttttctggc 
      ggctggagg atgaggctt gctttggcc agggcgttg 
      ttttcgcgc cccgatttg ctgcttttg gacgaaccg 
      acgaacatg ttggatatt aaggcgatt atttggctg 
      gagaattat ttacagaat tggccgaca acgttacta 
      gtggtttcc cacgatcga aattttcta gatacggtc 
      ccgacggat attttgcac ttgcattcg caaaggata 
      gatgcctac aggggaaat tatgaacag tttgagaaa 
      actaaaacc gagaaacta aaaaatcag cagagagag 
      tacgaggcg caaatgcag cacagacaa cacgtccaa 
      gtgttcatt gataagttt aggtacaat gccaacaga 
      gcggcctca gtgcagtca aaaatcaaa ttgttggat 
      aaattacct gaattgaag ccaatagaa aaggaaact 
      gctgtagtt ttgaaattc ccagatacc gaacctctc 
      tcacccccc attttgcaa cttaacgaa gtttcgttt 
      ggttacaac aaagataga ctcatattt accaacgtt 
      aatctaggg gctaccatg gattccagg atatgtatt 
      gtcggtgac aacggtgct ggtaaaacc accctcctc 
      aaaatcatc atgggcctc ctgtcccca tctgcgggc 
      atgctccac gtccatcga aacctaaaa ttcggctac 
      ttcagccag caccacgtg gaccaactc gacatgaac 
      gtcaactcg gtcgagctc ctccaaagc acatatccc 
      ggaaaacca atcgaggaa tatagacgc cagcttggg 
      agttttggc gtctcgggc gacctcgcc ctccaaacc 
      gtcgccagt ttatcaggg gggcaaaaa tctcgagtc 
      gcttttgcc cgaatgtgc atgggtaac ccaaatttc 
      ctcgtactt gacgaaccc accaatcat ttagatatc 
      gaaacaatt gaagcttta gggaatgcg atagtcaaa 
      ttcaccggc ggtgtgata cttgtgtcg cacgacgag 
      cggcttatt cgcaaagtg tgcaaggag ttgtgggtt 
      tgtggtaat ggctctgtg cgaagtata gaaggtttt 
      gatgagtac agacgactt gtcgaacag gaactcgaa 
      agtcagtct aaataacga gtttacttt ttaatagag 
      aaatttgta taaatgtgc ctcctttgt ggaaaaact 
      atttatgat tttttgtta tatttataa tatattcat 
      gtttataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY