Status
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Gene information
General information
TC011923 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11621299..11623356
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011923
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05126

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber symmetry irregular - Number of animals showing the phenotype: 3

    Comment: Position & size of eggs of previtellogenic are altered.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011923 RA
      MIGGLFVYN HKGEVLISR VYRDDIGRN AVDAFRVNV 
      IHARQQVRS PVTNIARTS FFHIKRANI WIAAVTKQN 
      VNAAMVFEF LLKIIEVMQ SYFGKISEE NIKNNFVLI 
      YELLDEILD FGYPQNTDT GVLKTFITQ QGIKSATKE 
      EQAQITSQV TGQIGWRRE GIKYRRNEL FLDVLEYVN 
      LLMSPQGQV LSAHVAGKV VMKSYLSGM PECKFGIND 
      KIVMEAKGK GGLGSTSDS DQTRSGKPV VVIDDCQFH 
      QCVKLSKFE TEHSISFIP PDGEFELMR YRTTKDISL 
      PFRVIPLVR EVGRTKMEV KVVLKSNFK PSLLGQKIE 
      VKIPTPLNT SGVQLICLK GKAKYKASE NAIVWKIKR 
      MAGMKETQL SAEIELLET DTKKKWTRP PISMNFEVP 
      FAPSGFKVR YLKVFEPKL NYSDHDVIK WVRYIGRSG 
      LYETRC
    CDS sequence
    • >TC011923 RA
      atgatcggg gggttgttc gtgtacaac cacaagggc 
      gaagtcctc atttcccga gtttacagg gatgatatc 
      ggcagaaat gcggtcgat gcgtttcgg gttaatgtt 
      atacatgcc cgacaacaa gtccgctct ccggtcaca 
      aacatagca cgaacgtcc tttttccac attaagagg 
      gctaatatt tggattgcc gccgtaaca aagcaaaat 
      gtcaacgcc gccatggtt tttgagttc ttgttgaag 
      attatcgaa gtcatgcag tcgtatttt gggaaaatc 
      tctgaagag aatattaaa aataatttc gttcttatt 
      tatgaatta ctagacgaa atcctggac tttggttac 
      cctcaaaac accgatacc ggcgttttg aaaacgttc 
      ataacccaa caagggatc aaatcggcc actaaggaa 
      gaacaagct cagattacg tcacaggtt actggtcag 
      attggttgg aggcgtgag ggtatcaag tatcgtcga 
      aacgagttg tttttggat gttttggaa tatgttaac 
      ctacttatg tcgcctcaa ggccaggtt ctttcggcc 
      cacgtggcc ggtaaagtc gtgatgaag tcgtatttg 
      tcgggaatg cccgaatgc aagttcggg attaacgac 
      aagattgtg atggaagcc aaaggcaaa gggggctta 
      gggtcgacg tctgattcc gatcagacc cggtctggc 
      aaaccagtc gttgtaatt gacgattgc cagttccac 
      caatgcgtg aaattaagt aaattcgaa accgaacat 
      tccattagc tttattcca cctgacggt gaattcgaa 
      ttgatgaga tatcgtaca actaaagac atatcgttg 
      ccatttcgg gtaattccc ctcgtgcgt gaagtcggt 
      cgcaccaaa atggaggtc aaggtggtg ctgaaaagc 
      aacttcaag ccgtcgctt cttgggcaa aaaatcgaa 
      gttaaaatt ccaactccg ttgaacact tcgggagtt 
      caattgatt tgtttgaaa ggaaaggcg aaatacaaa 
      gcatcggaa aatgcgatt gtttggaag atcaaacgt 
      atggctgga atgaaggag acgcaatta tcggccgaa 
      atcgagctt ctggagacc gacacgaag aagaagtgg 
      acaagaccg ccgatttct atgaatttc gaagtaccg 
      ttcgcaccg tccggtttt aaagtacga tacttgaag 
      gttttcgag ccgaaattg aattactcg gatcatgat 
      gttattaaa tgggtgcgg tatatcgga aggagcgga 
      ctttacgaa actcgatgc taa
    mRNA sequence
    • >TC011923 RA
      tatcgcagt gacaagttg tcagtgggt gtatgatgg 
      ccaagtaaa ataaaaacc gttcggctt taactaata 
      attccgtta aatcacaaa agtgtgtgc gtaaaaagc 
      ctctaatga cttcacaat tcggtcatt aggcgagag 
      ctgagccgg cagaatgat cggggggtt gttcgtgta 
      caaccacaa gggcgaagt cctcatttc ccgagttta 
      cagggatga tatcggcag aaatgcggt cgatgcgtt 
      tcgggttaa tgttataca tgcccgaca acaagtccg 
      ctctccggt cacaaacat agcacgaac gtccttttt 
      ccacattaa gagggctaa tatttggat tgccgccgt 
      aacaaagca aaatgtcaa cgccgccat ggtttttga 
      gttcttgtt gaagattat cgaagtcat gcagtcgta 
      ttttgggaa aatctctga agagaatat taaaaataa 
      tttcgttct tatttatga attactaga cgaaatcct 
      ggactttgg ttaccctca aaacaccga taccggcgt 
      tttgaaaac gttcataac ccaacaagg gatcaaatc 
      ggccactaa ggaagaaca agctcagat tacgtcaca 
      ggttactgg tcagattgg ttggaggcg tgagggtat 
      caagtatcg tcgaaacga gttgttttt ggatgtttt 
      ggaatatgt taacctact tatgtcgcc tcaaggcca 
      ggttctttc ggcccacgt ggccggtaa agtcgtgat 
      gaagtcgta tttgtcggg aatgcccga atgcaagtt 
      cgggattaa cgacaagat tgtgatgga agccaaagg 
      caaaggggg cttagggtc gacgtctga ttccgatca 
      gacccggtc tggcaaacc agtcgttgt aattgacga 
      ttgccagtt ccaccaatg cgtgaaatt aagtaaatt 
      cgaaaccga acattccat tagctttat tccacctga 
      cggtgaatt cgaattgat gagatatcg tacaactaa 
      agacatatc gttgccatt tcgggtaat tcccctcgt 
      gcgtgaagt cggtcgcac caaaatgga ggtcaaggt 
      ggtgctgaa aagcaactt caagccgtc gcttcttgg 
      gcaaaaaat cgaagttaa aattccaac tccgttgaa 
      cacttcggg agttcaatt gatttgttt gaaaggaaa 
      ggcgaaata caaagcatc ggaaaatgc gattgtttg 
      gaagatcaa acgtatggc tggaatgaa ggagacgca 
      attatcggc cgaaatcga gcttctgga gaccgacac 
      gaagaagaa gtggacaag accgccgat ttctatgaa 
      tttcgaagt accgttcgc accgtccgg ttttaaagt 
      acgatactt gaaggtttt cgagccgaa attgaatta 
      ctcggatca tgatgttat taaatgggt gcggtatat 
      cggaaggag cggacttta cgaaactcg atgctaaat 
      tttgaggaa caacatttt atttcaatt atgtttatt 
      ttgtgttat ctaatttag tagttagtt gcttttttt 
      atcgatggt ttacatcat gtcggatcg aatattatt 
      tcttgctct caaatactc aaggcaagc ttgatgttg 
      gaatagaag tgttatgca aaagtatgc tctcaataa 
      ttttatgtg aaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY