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Gene information
General information
TC011922 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11624901..11629233
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011922
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05125

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen posterior everted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011922 RA
      MLLASRVWT CLCFMIKKQ TRAIIQHQS VKYDLFPLS 
      PLSRHRLSI VKRKVLVLD LDETLIHSH HDGVVRQTV 
      RPGTPPDFV LKVVIDRHP VRFFVHKRP HVDFFLDIV 
      SQWYELVVF TASMEIYGA AVADKLDAG RGILQRRFY 
      RQHCTPDLG SYTKDLGAI CNDLSSVFI LDNSPGAYR 
      AYPDNAIPI KSWFSDPTD VALLNLLPV LDALRFTAD 
      VRSVLSRNL HLHRLCRPN RTDIPVNSS FTKIHRIET 
      PSKIVDQVE NCIDNIVTQ VQEFDSTKD SKKFKSRIP 
      QRMCN
    CDS sequence
    • >TC011922 RA
      atgcttcta gcatcgaga gtttggaca tgtttgtgt 
      tttatgata aaaaagcaa actagagcg ataattcag 
      catcaatcg gtcaaatat gatttgttc cccttatca 
      ccgctatcg aggcatagg cttagtata gtaaaacgg 
      aaagtgctc gtcctcgac ttggacgag actttgatc 
      cactcgcac cacgatggg gtggtgcgc caaaccgtg 
      agaccgggg actcccccg gatttcgta cttaaagtg 
      gtcatagac aggcatccc gtgcgattt tttgtccat 
      aaaagacca catgtagat ttcttccta gatatagtt 
      tcccagtgg tacgaattg gtcgttttc acggccagt 
      atggagatt tacggcgct gccgtagct gataagttg 
      gacgcaggt cggggaatc ctccagagg aggttttac 
      aggcagcac tgcaccccc gatctcggc tcgtacacc 
      aaagatttg ggtgcgatc tgcaacgat ctctccagt 
      gtttttatt ttggataac agtccgggg gcctaccgc 
      gcctaccct gacaatgca atcccaata aaatcgtgg 
      ttctcagat cccaccgac gtggcgttg ctcaatctg 
      cttccggtt ttggacgca ttgaggttc acagcggac 
      gtccgatcc gtcttgtcg cggaatctc cacttgcac 
      cggctttgc cgtcctaat cgaaccgat atccccgtt 
      aacagcagt ttcactaaa attcatcga atcgaaact 
      cctagtaaa atcgttgat caagtcgaa aattgtatc 
      gataatatc gtgacacaa gtccaagaa ttcgattca 
      accaaagac agtaaaaaa ttcaagtcg cgcattccg 
      cagcgaatg tgcaactga
    mRNA sequence
    • >TC011922 RA
      catgcgctg tcaggcgag tgtcaaacg ctctctggg 
      tcgatttta atggcggtt aacaaatcg ggtaaaagt 
      taaaatgtg ataagcaac gtgaaataa caaatcacc 
      aaaacccgc tgtatcctc cttgtgtct aaattgtgg 
      caaagtgta gaaaaaaat acatttcaa tgttgaagc 
      aattacaaa tgggactac gagcattca tgcttctag 
      catcgagag tttggacat gtttgtgtt ttatgataa 
      aaaagcaaa ctagagcga taattcagc atcaatcgg 
      tcaaatatg atttgttcc ccttatcac cgctatcga 
      ggcataggc ttagtatag taaaacgga aagtgctcg 
      tcctcgact tggacgaga ctttgatcc actcgcacc 
      acgatgggg tggtgcgcc aaaccgtga gaccgggga 
      ctcccccgg atttcgtac ttaaagtgg tcatagaca 
      ggcatcccg tgcgatttt ttgtccata aaagaccac 
      atgtagatt tcttcctag atatagttt cccagtggt 
      acgaattgg tcgttttca cggccagta tggagattt 
      acggcgctg ccgtagctg ataagttgg acgcaggtc 
      ggggaatcc tccagagga ggttttaca ggcagcact 
      gcacccccg atctcggct cgtacacca aagatttgg 
      gtgcgatct gcaacgatc tctccagtg tttttattt 
      tggataaca gtccggggg cctaccgcg cctaccctg 
      acaatgcaa tcccaataa aatcgtggt tctcagatc 
      ccaccgacg tggcgttgc tcaatctgc ttccggttt 
      tggacgcat tgaggttca cagcggacg tccgatccg 
      tcttgtcgc ggaatctcc acttgcacc ggctttgcc 
      gtcctaatc gaaccgata tccccgtta acagcagtt 
      tcactaaaa ttcatcgaa tcgaaactc ctagtaaaa 
      tcgttgatc aagtcgaaa attgtatcg ataatatcg 
      tgacacaag tccaagaat tcgattcaa ccaaagaca 
      gtaaaaaat tcaagtcgc gcattccgc agcgaatgt 
      gcaactgaa ttttggaaa ttgtgttgt gattttcgt 
      tttttgtag attgaacga aattatttg tagcaaata 
      aaccgttac gagacattg tttttctgt tctttaacg 
      ctcgattcg aatgatttt atttgtttt atttttgtt 
      tgtgcgggg acagctgat aacagtatt tgaatgcag 
      gtttctcgt gctcaaact ggacaatag tggtgtctg 
      tcacccaca cttatcttc cacactcct accaacacc 
      ggaagtaac cacccaaac agaactgtt attactttt 
      aattgtttc gtttgaatt cagttattt attttattg 
      tttgtgtag tctattgag ttctatcat tccatgatt 
      agcggaatt attatttag accaaagac cccgaaatt 
      gtttggctt attgtacag cattttgtt cagctcatt 
      gtacataaa tagcgtaag ttttccaag actctgatc 
      ttattttct gcatcagaa aataattat acgaaacaa 
      taattttgt gttcaaaat tattgggac tagattaga 
      taataatgt atataggta gttcagctt ggctaaaat 
      taggcaaag cttcgattt ttggcacca attattgtc 
      atatcataa actcggtgc ctgagttga acaccacat 
      ttcgtggcc aatttttat ttacagttg aactgtact 
      taagcgccc tcacgcggc caatttttt taagtaagc 
      tttgccaac tccgttggg agcttgcca acagttttg 
      gtttgtaaa taattgtaa attgtgacg ctgtcttaa 
      tgttattgt tgaaactac tagataagg tgctacttc 
      acacttttg tgaatagtc aaatagggt gtgctgtct 
      ttagaaaaa attgttttt ttttgcctg gtaggttgt 
      acttacggc aagttttgg aatagttaa ttgttagtt 
      tctaggtac aaatcctaa cctttgtaa atacatgtt 
      ttagcgttt aaactgtac agtaactct atacaaact 
      tggaacaaa gtacctaat aaatgacct tttacagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY