General information | |||
TC011917 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 11667137..11669372 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011917 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05124 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC011917 RA
MKNKNFIRF LFGIIIVSL FLIIFRQHK PETIQNIVS TTHQQIRNF KDTFRDVDS KTLAADEKY LTLLGFTDS PRLYPHDIW RNTSLPVIV TYVMEGQES QAVGLINNV GRVLPNNTI LVYNLGLGN YGLKTLLNY CNNSRCQAL TFSLANFPS HVDEESIRA YRPLVIQDA LQRTGAVLF LECGYRFSR HLTHDVIMD LFEKRAKSQ GIVTWPMSL KNPVSSLTH KKMFQYFHT DAENFLFLQ MVNADVLLI VNTESVHNE IMLPWVKCA LTQDCIVPI GAQSAGCRF DKKPQYRYS GCHSYDTSA LNIVLGLKF NMDGSRYMF DESEKLFET VELSKAEAL LREFEQNIT TEGRFMSFD T
- >TC011917 RA
atgaagaat aagaatttc atcaggttt ctgttcgga ataatcata gtcagtttg tttttgatt atattcaga cagcataag cctgagacg atacagaac atcgtttct acaacccac caacaaatc cgcaatttc aaggacacg ttccgagat gttgactcg aagacgctt gccgccgac gagaaatat ctgactttg ttggggttc acagacagc ccccgactg tatccacat gacatctgg aggaacact tcactgcca gtcattgtc acgtatgtt atggaaggg caggaaagc caagctgtg ggacttatt aataacgta ggaagggtt ctgccaaac aatacaatt ctagtttat aaccttggt ttaggaaat tatggccta aaaacgctc ttaaattat tgcaacaac agccgatgt caggccttg acgttcagt ttggccaat tttccctcg cacgtggac gaggagagc atacgggcc tacaggccc cttgtcatc caggacgcc ctccagcgc accggcgcc gtgttgttc ctggagtgc gggtaccgc ttttcgcgg cacttgacc cacgacgtc atcatggac ctgtttgag aagcgggcg aagtcccag ggcatcgtc acgtggccc atgtcgcta aagaaccca gtttcgagt ctgacgcac aagaagatg ttccagtac ttccacacc gacgccgag aatttcctt ttcttgcag atggtgaac gcggacgtg cttttgatc gtcaacacg gagtcggtg cacaacgaa atcatgctg ccctgggtc aagtgcgcg ctgacccag gactgcatc gttccgatt ggggcgcag tcggccggg tgtcgcttc gacaaaaag ccccaatat cgatattcc gggtgccat agctacgac acgtcagcg ctcaatata gttctagga ctgaaattt aacatggac ggcagtcgg tacatgttt gacgagtcg gagaagctc ttcgagacg gttgagttg agcaaagcc gaggctctc ttgagagaa ttcgaacaa aatattact actgaaggg cgattcatg tctttcgac acgtaa
- >TC011917 RA
acctcttca agtgatttt atcaaaaat ttgtataaa ttatggaaa ttcgcggct tgtgtgcga gttagcagg cgttttttt gacccattt ggcccccaa agtgcgttt ttatcatgt gaatgcaag cttgagact tgatggaaa tcgtgccgt gccccaatg aagaataag aatttcatc aggtttctg ttcggaata atcatagtc agtttgttt ttgattata ttcagacag cataagcct gagacgata cagaacatc gtttctaca acccaccaa caaatccgc aatttcaag gacacgttc cgagatgtt gactcgaag acgcttgcc gccgacgag aaatatctg actttgttg gggttcaca gacagcccc cgactgtat ccacatgac atctggagg aacacttca ctgccagtc attgtcacg tatgttatg gaagggcag gaaagccaa gctgtggga cttattaat aacgtagga agggttctg ccaaacaat acaattcta gtttataac cttggttta ggaaattat ggcctaaaa acgctctta aattattgc aacaacagc cgatgtcag gccttgacg ttcagtttg gccaatttt ccctcgcac gtggacgag gagagcata cgggcctac aggcccctt gtcatccag gacgccctc cagcgcacc ggcgccgtg ttgttcctg gagtgcggg taccgcttt tcgcggcac ttgacccac gacgtcatc atggacctg tttgagaag cgggcgaag tcccagggc atcgtcacg tggcccatg tcgctaaag aacccagtt tcgagtctg acgcacaag aagatgttc cagtacttc cacaccgac gccgagaat ttccttttc ttgcagatg gtgaacgcg gacgtgctt ttgatcgtc aacacggag tcggtgcac aacgaaatc atgctgccc tgggtcaag tgcgcgctg acccaggac tgcatcgtt ccgattggg gcgcagtcg gccgggtgt cgcttcgac aaaaagccc caatatcga tattccggg tgccatagc tacgacacg tcagcgctc aatatagtt ctaggactg aaatttaac atggacggc agtcggtac atgtttgac gagtcggag aagctcttc gagacggtt gagttgagc aaagccgag gctctcttg agagaattc gaacaaaat attactact gaagggcga ttcatgtct ttcgacacg taaatttta ggtcggacc aaattcagt gtgataata agcgtaaag ttattcctt tttgaaacc aaataatgc caagcactc gatttcgtt tcttgaacg gcgcagttg gccccgaac ttgtgattt agttggtat ttgctgtac aaaacgcat cttccttgt cgtttaggg cgcttgcag ccctttcag gtcacgtgg ctcggtatt ccaccacct tgtcgtttc tttgactgt acatagtta tttattgtt tctgttgta attgaagtg cttatttaa aaaataaaa aacacaata caaaacgtt ttattattt aaatacggt tggacacag cctccaatt attattata ttatcctcc tgcacagcc ttcttgatc aatgaagga tgaacgaga tgagccact taatctggc gattacggt aacacgaac agtcttatg actgattct cctaaacac ataaaacga tcagaaaga atatgtttt tttaacatt ttattggcc atttttaag cgtctactg aaatttgac gacattagc attttttat taaagaaga aggaattat
