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Gene information
General information
TC011893 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12146481..12150292
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011893
    iBeetle screen iB_05122

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011893 RA
      MAREESRGK RPFKLWDGR RSVRKGLVV GSLDELVQR 
GRDKLGVSI GEPVRLVLE SDGTQVEDA EYFRTLPAN 
TILLLLRPG ERWLPAGVD VIRAVAISA IPKIVCETI 
HALELHDET PSWKIMDNK GRVTVVLHW DQRSAPTRT 
SSETPSPAP GKFSPSKRP SLVIQTSVG GKKETLIND 
VYVPPGQPQ PRYSSPQIT VINHDDVKP VHRLSKQGS 
SLESATIHI HTPECAHHA HMPRAGSPQ SNGAIECDF 
HCCALHEEG RKIAVHKSV ATSPIQDAQ QQTSPQPPS 
SRHVRFLDI EGGGRHGGG QSEHESSES ETENTVMED 
EAVTSEKFL LLIDQLSVD QKRHLSIKD IGIILERLS 
SKILDVERL DRESESDDC YNWTIKATI RGDVLRELG 
VIYNGNYYA ISEHPGYKE ENEGVIEEA EEEEEEEDR 
L
    CDS sequence
    • >TC011893 RA
      atggcaaga gaggaatcc cgcggaaag agacccttc 
aagctatgg gacgggcgt cgtagtgtg cgcaagggg 
ctggtggtg gggagcctc gacgagcta gtgcagcgc 
ggaagagac aagctcggc gtctccatc ggagagccg 
gtgcgattg gtgttggag agcgacggt acccaggtc 
gaagacgcc gagtacttt cggacgcta cctgccaac 
accatcctt ctgctgctg aggccgggc gagcgatgg 
ctgcccgcc ggcgtggac gtcatccga gcagtagcc 
atctcagcg atcccgaag atcgtgtgc gaaacgatc 
cacgctttg gagctgcac gatgaaacg ccctcgtgg 
aagatcatg gacaacaag ggccgcgtc accgtcgtg 
cttcactgg gaccagcgt tcggccccg acgaggacc 
tcctcggag acgccgtcg cccgccccc ggcaagttc 
tcgccgagc aagcggccc tcgctcgtc atccagact 
tccgtgggg ggcaagaag gagactctc atcaatgat 
gtgtacgtg cctccaggc cagccccaa cctcgctac 
tccagccct cagatcact gtgataaac cacgacgac 
gttaaaccc gttcatcgg ctgtccaag cagggcagc 
tccttggag agcgccacc attcacatc catacgccc 
gagtgcgcc caccacgct cacatgccc cgcgccggt 
agcccccag agcaacggc gcgatcgag tgcgacttc 
cactgctgt gccctccac gaggagggc cggaagata 
gccgtgcac aagagcgtg gccacgtcg ccgattcaa 
gatgctcaa cagcagacc tcaccgcaa ccgccatca 
tctagacac gtgcgtttt ttagacatt gaaggtggc 
ggccgacac ggcggcggg cagtccgaa cacgagagc 
tccgaaagc gaaacggag aacaccgtg atggaggac 
gaggccgtc acctctgaa aaattcctg ctgttgatc 
gaccaattg agcgtcgat cagaaacga catctgagc 
atcaaagat atcgggatc atactcgaa aggctcagc 
tcgaaaatt ttggacgtt gaaaggtta gatcgcgag 
agcgaaagc gacgattgc tacaactgg accatcaaa 
gcgacgatt cgcggcgat gttctgcgc gaattgggc 
gtcatctac aacggcaac tattacgcc atttcggaa 
catccaggt tacaaggag gagaacgag ggggtcatt 
gaggaggca gaagaagaa gaagaggaa gaagacagg 
ctgtga
    mRNA sequence
    • >TC011893 RA
      atcgatcgg acgctaggg ggcgcgttt gtcggggca 
gtttgcagg gttgctgct tgtaggggt cacagatag 
tccgattga attttggtc gagaatgga caaaaagag 
acggatcgg gctgagaat atgcggtgc cgtggttta 
gtgacagtg ccggtgcca tagaggagt gcgcgggcc 
agcggcggg acgacggcc gcaccataa gagatgccg 
tgatggcaa gagaggaat cccgcggaa agagaccct 
tcaagctat gggacgggc gtcgtagtg tgcgcaagg 
ggctggtgg tggggagcc tcgacgagc tagtgcagc 
gcggaagag acaagctcg gcgtctcca tcggagagc 
cggtgcgat tggtgttgg agagcgacg gtacccagg 
tcgaagacg ccgagtact ttcggacgc tacctgcca 
acaccatcc ttctgctgc tgaggccgg gcgagcgat 
ggctgcccg ccggcgtgg acgtcatcc gagcagtag 
ccatctcag cgatcccga agatcgtgt gcgaaacga 
tccacgctt tggagctgc acgatgaaa cgccctcgt 
ggaagatca tggacaaca agggccgcg tcaccgtcg 
tgcttcact gggaccagc gttcggccc cgacgagga 
cctcctcgg agacgccgt cgcccgccc ccggcaagt 
tctcgccga gcaagcggc cctcgctcg tcatccaga 
cttccgtgg ggggcaaga aggagactc tcatcaatg 
atgtgtacg tgcctccag gccagcccc aacctcgct 
actccagcc ctcagatca ctgtgataa accacgacg 
acgttaaac ccgttcatc ggctgtcca agcagggca 
gctccttgg agagcgcca ccattcaca tccatacgc 
ccgagtgcg cccaccacg ctcacatgc cccgcgccg 
gtagccccc agagcaacg gcgcgatcg agtgcgact 
tccactgct gtgccctcc acgaggagg gccggaaga 
tagccgtgc acaagagcg tggccacgt cgccgattc 
aagatgctc aacagcaga cctcaccgc aaccgccat 
catctagac acgtgcgtt ttttagaca ttgaaggtg 
gcggccgac acggcggcg ggcagtccg aacacgaga 
gctccgaaa gcgaaacgg agaacaccg tgatggagg 
acgaggccg tcacctctg aaaaattcc tgctgttga 
tcgaccaat tgagcgtcg atcagaaac gacatctga 
gcatcaaag atatcggga tcatactcg aaaggctca 
gctcgaaaa ttttggacg ttgaaaggt tagatcgcg 
agagcgaaa gcgacgatt gctacaact ggaccatca 
aagcgacga ttcgcggcg atgttctgc gcgaattgg 
gcgtcatct acaacggca actattacg ccatttcgg 
aacatccag gttacaagg aggagaacg agggggtca 
ttgaggagg cagaagaag aagaagagg aagaagaca 
ggctgtgaa ctgacagct aacgactgc caatgtcat 
aatgcacaa aagcagact ctagtaata agcacaggt 
gcaaaatat cacatatcg gatgtgaac tttgtaaac 
cgtactccg tactgactt gccatggtg atcgatcct 
tcttaaaac acgcgttgc gagttgtgc ggggcgacg 
actgccaca ccgtctcgt agtctaata aacaaaaaa 
tattgatac ctattggaa attaaatga gaaaaagat 
aaaaccaca aaaaaatca ttaaggaaa acataggaa 
aacaaaatt aacgacacg acttgacta gttgtatag 
ggttgacaa tcgattctc gatcggtgc gattcgatt 
cgttcgttt aaacgagtt tttaagtca ccgacgatg 
attacacac gaatactga tgcaaaaga acatgaaaa 
attgtgttt gtataggta tccttgtta atcatgtaa 
atcatttat aaataataa aactattcg cttagatgg 
ttgtttgtt gtgcgcgcg agtacgcga aacgcgtcg 
caggactct tctcatgat gactttcat tataacatt 
attacactc aactatcca ccgtggacg tggctttag 
ccgcacggg ggaccccta ggattatat ataaacgct 
ttgtacttt ttataatta tgtggattg tacgagtac 
ttagccaat ttaaagtta attattttt aagaaatta 
ttagcccag gtcatttag atgagacat gatgattca 
gtatttata taaaatata ttatataag tatataaga 
ggaagttgt ttaggatat ttgagatgt ttataactg 
ggttactcg atgattgat tgattgatt gattgtata 
ttgctcaat tgtcctttt gtttttgta atcattaac 
caccatcgg tcgggtatg ttgtgataa gatattgcc 
gagcgcgat atatattat atatattat tgtaattaa 
gtatatata atattgaag aaaaaagaa agctagggt 
tggggggtg aaacgggcg aatcaattt gggacactt 
ggtgatttt ttcatgaga atggaaagc aaacgtagg 
tctatatta gataaatta attataaag tgaataaaa 
ctgtgcaaa aaaattgaa aaaaaattt tttttggaa 
tttgatctg ggggtgcgt ttcggctcc gcggtccgt 
tcgcacgcc gcttgtctt gttgaggcg tgcctcgga 
gcggaatcg catcgatgg tcgatggac gcgtcccag 
caaacaaac agcaaagaa caaacgacg tcgagtcca 
tcctccgcc aaacatgtt ctaaaatct aattaccac 
tgccatcaa cctgtaaac cacgagaaa attgcaaaa 
ttccgggtg atcagctga cgggacggg acgggacga 
cgggcgggg cgccgcccc ccgccggag gcccccatc 
gatcgccgg tgcaccatc gggaccccg tcttggtgg 
tgcacggcc ccgaaaacg ggaactgca ctgcgaaac 
ccgagtcgg gggcgggcc gggagcggc cgggtcgag 
cggccgttg actcctgcg aggggagga cgcgctcct 
gtcacggca cactgccct acttccgag cggaggggc 
gaacctatc attcggcct ccggtgtta ttatgcaat 
acgtgataa ttctcatca cttccatca gacgagcca 
ccagctccg ggtacttcg ttcgtcgtg cgagtgaat 
tatttatta cggcgtcac taacacatt attcatcgc 
ggatttttc attcggttt tcgacgtat tattataat 
tgtcgttcg ttcgttcgt tcgttcgtt ccttcggtc 
atcatcaag caaaaaaag agcaaaaac gaaacgaaa 
aaactattc actcatcgt ttctacatt gttcatcat 
cattgtttt aaataggaa aattaatgt taattagat 
aaacaagaa agaactatt atttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY