Status
Please wait ...
Gene information
General information
TC011893 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12146481..12150292
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011893
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05122

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011893 RA
      MAREESRGK RPFKLWDGR RSVRKGLVV GSLDELVQR 
      GRDKLGVSI GEPVRLVLE SDGTQVEDA EYFRTLPAN 
      TILLLLRPG ERWLPAGVD VIRAVAISA IPKIVCETI 
      HALELHDET PSWKIMDNK GRVTVVLHW DQRSAPTRT 
      SSETPSPAP GKFSPSKRP SLVIQTSVG GKKETLIND 
      VYVPPGQPQ PRYSSPQIT VINHDDVKP VHRLSKQGS 
      SLESATIHI HTPECAHHA HMPRAGSPQ SNGAIECDF 
      HCCALHEEG RKIAVHKSV ATSPIQDAQ QQTSPQPPS 
      SRHVRFLDI EGGGRHGGG QSEHESSES ETENTVMED 
      EAVTSEKFL LLIDQLSVD QKRHLSIKD IGIILERLS 
      SKILDVERL DRESESDDC YNWTIKATI RGDVLRELG 
      VIYNGNYYA ISEHPGYKE ENEGVIEEA EEEEEEEDR 
      L
    CDS sequence
    • >TC011893 RA
      atggcaaga gaggaatcc cgcggaaag agacccttc 
      aagctatgg gacgggcgt cgtagtgtg cgcaagggg 
      ctggtggtg gggagcctc gacgagcta gtgcagcgc 
      ggaagagac aagctcggc gtctccatc ggagagccg 
      gtgcgattg gtgttggag agcgacggt acccaggtc 
      gaagacgcc gagtacttt cggacgcta cctgccaac 
      accatcctt ctgctgctg aggccgggc gagcgatgg 
      ctgcccgcc ggcgtggac gtcatccga gcagtagcc 
      atctcagcg atcccgaag atcgtgtgc gaaacgatc 
      cacgctttg gagctgcac gatgaaacg ccctcgtgg 
      aagatcatg gacaacaag ggccgcgtc accgtcgtg 
      cttcactgg gaccagcgt tcggccccg acgaggacc 
      tcctcggag acgccgtcg cccgccccc ggcaagttc 
      tcgccgagc aagcggccc tcgctcgtc atccagact 
      tccgtgggg ggcaagaag gagactctc atcaatgat 
      gtgtacgtg cctccaggc cagccccaa cctcgctac 
      tccagccct cagatcact gtgataaac cacgacgac 
      gttaaaccc gttcatcgg ctgtccaag cagggcagc 
      tccttggag agcgccacc attcacatc catacgccc 
      gagtgcgcc caccacgct cacatgccc cgcgccggt 
      agcccccag agcaacggc gcgatcgag tgcgacttc 
      cactgctgt gccctccac gaggagggc cggaagata 
      gccgtgcac aagagcgtg gccacgtcg ccgattcaa 
      gatgctcaa cagcagacc tcaccgcaa ccgccatca 
      tctagacac gtgcgtttt ttagacatt gaaggtggc 
      ggccgacac ggcggcggg cagtccgaa cacgagagc 
      tccgaaagc gaaacggag aacaccgtg atggaggac 
      gaggccgtc acctctgaa aaattcctg ctgttgatc 
      gaccaattg agcgtcgat cagaaacga catctgagc 
      atcaaagat atcgggatc atactcgaa aggctcagc 
      tcgaaaatt ttggacgtt gaaaggtta gatcgcgag 
      agcgaaagc gacgattgc tacaactgg accatcaaa 
      gcgacgatt cgcggcgat gttctgcgc gaattgggc 
      gtcatctac aacggcaac tattacgcc atttcggaa 
      catccaggt tacaaggag gagaacgag ggggtcatt 
      gaggaggca gaagaagaa gaagaggaa gaagacagg 
      ctgtga
    mRNA sequence
    • >TC011893 RA
      atcgatcgg acgctaggg ggcgcgttt gtcggggca 
      gtttgcagg gttgctgct tgtaggggt cacagatag 
      tccgattga attttggtc gagaatgga caaaaagag 
      acggatcgg gctgagaat atgcggtgc cgtggttta 
      gtgacagtg ccggtgcca tagaggagt gcgcgggcc 
      agcggcggg acgacggcc gcaccataa gagatgccg 
      tgatggcaa gagaggaat cccgcggaa agagaccct 
      tcaagctat gggacgggc gtcgtagtg tgcgcaagg 
      ggctggtgg tggggagcc tcgacgagc tagtgcagc 
      gcggaagag acaagctcg gcgtctcca tcggagagc 
      cggtgcgat tggtgttgg agagcgacg gtacccagg 
      tcgaagacg ccgagtact ttcggacgc tacctgcca 
      acaccatcc ttctgctgc tgaggccgg gcgagcgat 
      ggctgcccg ccggcgtgg acgtcatcc gagcagtag 
      ccatctcag cgatcccga agatcgtgt gcgaaacga 
      tccacgctt tggagctgc acgatgaaa cgccctcgt 
      ggaagatca tggacaaca agggccgcg tcaccgtcg 
      tgcttcact gggaccagc gttcggccc cgacgagga 
      cctcctcgg agacgccgt cgcccgccc ccggcaagt 
      tctcgccga gcaagcggc cctcgctcg tcatccaga 
      cttccgtgg ggggcaaga aggagactc tcatcaatg 
      atgtgtacg tgcctccag gccagcccc aacctcgct 
      actccagcc ctcagatca ctgtgataa accacgacg 
      acgttaaac ccgttcatc ggctgtcca agcagggca 
      gctccttgg agagcgcca ccattcaca tccatacgc 
      ccgagtgcg cccaccacg ctcacatgc cccgcgccg 
      gtagccccc agagcaacg gcgcgatcg agtgcgact 
      tccactgct gtgccctcc acgaggagg gccggaaga 
      tagccgtgc acaagagcg tggccacgt cgccgattc 
      aagatgctc aacagcaga cctcaccgc aaccgccat 
      catctagac acgtgcgtt ttttagaca ttgaaggtg 
      gcggccgac acggcggcg ggcagtccg aacacgaga 
      gctccgaaa gcgaaacgg agaacaccg tgatggagg 
      acgaggccg tcacctctg aaaaattcc tgctgttga 
      tcgaccaat tgagcgtcg atcagaaac gacatctga 
      gcatcaaag atatcggga tcatactcg aaaggctca 
      gctcgaaaa ttttggacg ttgaaaggt tagatcgcg 
      agagcgaaa gcgacgatt gctacaact ggaccatca 
      aagcgacga ttcgcggcg atgttctgc gcgaattgg 
      gcgtcatct acaacggca actattacg ccatttcgg 
      aacatccag gttacaagg aggagaacg agggggtca 
      ttgaggagg cagaagaag aagaagagg aagaagaca 
      ggctgtgaa ctgacagct aacgactgc caatgtcat 
      aatgcacaa aagcagact ctagtaata agcacaggt 
      gcaaaatat cacatatcg gatgtgaac tttgtaaac 
      cgtactccg tactgactt gccatggtg atcgatcct 
      tcttaaaac acgcgttgc gagttgtgc ggggcgacg 
      actgccaca ccgtctcgt agtctaata aacaaaaaa 
      tattgatac ctattggaa attaaatga gaaaaagat 
      aaaaccaca aaaaaatca ttaaggaaa acataggaa 
      aacaaaatt aacgacacg acttgacta gttgtatag 
      ggttgacaa tcgattctc gatcggtgc gattcgatt 
      cgttcgttt aaacgagtt tttaagtca ccgacgatg 
      attacacac gaatactga tgcaaaaga acatgaaaa 
      attgtgttt gtataggta tccttgtta atcatgtaa 
      atcatttat aaataataa aactattcg cttagatgg 
      ttgtttgtt gtgcgcgcg agtacgcga aacgcgtcg 
      caggactct tctcatgat gactttcat tataacatt 
      attacactc aactatcca ccgtggacg tggctttag 
      ccgcacggg ggaccccta ggattatat ataaacgct 
      ttgtacttt ttataatta tgtggattg tacgagtac 
      ttagccaat ttaaagtta attattttt aagaaatta 
      ttagcccag gtcatttag atgagacat gatgattca 
      gtatttata taaaatata ttatataag tatataaga 
      ggaagttgt ttaggatat ttgagatgt ttataactg 
      ggttactcg atgattgat tgattgatt gattgtata 
      ttgctcaat tgtcctttt gtttttgta atcattaac 
      caccatcgg tcgggtatg ttgtgataa gatattgcc 
      gagcgcgat atatattat atatattat tgtaattaa 
      gtatatata atattgaag aaaaaagaa agctagggt 
      tggggggtg aaacgggcg aatcaattt gggacactt 
      ggtgatttt ttcatgaga atggaaagc aaacgtagg 
      tctatatta gataaatta attataaag tgaataaaa 
      ctgtgcaaa aaaattgaa aaaaaattt tttttggaa 
      tttgatctg ggggtgcgt ttcggctcc gcggtccgt 
      tcgcacgcc gcttgtctt gttgaggcg tgcctcgga 
      gcggaatcg catcgatgg tcgatggac gcgtcccag 
      caaacaaac agcaaagaa caaacgacg tcgagtcca 
      tcctccgcc aaacatgtt ctaaaatct aattaccac 
      tgccatcaa cctgtaaac cacgagaaa attgcaaaa 
      ttccgggtg atcagctga cgggacggg acgggacga 
      cgggcgggg cgccgcccc ccgccggag gcccccatc 
      gatcgccgg tgcaccatc gggaccccg tcttggtgg 
      tgcacggcc ccgaaaacg ggaactgca ctgcgaaac 
      ccgagtcgg gggcgggcc gggagcggc cgggtcgag 
      cggccgttg actcctgcg aggggagga cgcgctcct 
      gtcacggca cactgccct acttccgag cggaggggc 
      gaacctatc attcggcct ccggtgtta ttatgcaat 
      acgtgataa ttctcatca cttccatca gacgagcca 
      ccagctccg ggtacttcg ttcgtcgtg cgagtgaat 
      tatttatta cggcgtcac taacacatt attcatcgc 
      ggatttttc attcggttt tcgacgtat tattataat 
      tgtcgttcg ttcgttcgt tcgttcgtt ccttcggtc 
      atcatcaag caaaaaaag agcaaaaac gaaacgaaa 
      aaactattc actcatcgt ttctacatt gttcatcat 
      cattgtttt aaataggaa aattaatgt taattagat 
      aaacaagaa agaactatt atttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY