| General information | |||
| TC011893 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12146481..12150292 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011893 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05122 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC011893 RA
MAREESRGK RPFKLWDGR RSVRKGLVV GSLDELVQR GRDKLGVSI GEPVRLVLE SDGTQVEDA EYFRTLPAN TILLLLRPG ERWLPAGVD VIRAVAISA IPKIVCETI HALELHDET PSWKIMDNK GRVTVVLHW DQRSAPTRT SSETPSPAP GKFSPSKRP SLVIQTSVG GKKETLIND VYVPPGQPQ PRYSSPQIT VINHDDVKP VHRLSKQGS SLESATIHI HTPECAHHA HMPRAGSPQ SNGAIECDF HCCALHEEG RKIAVHKSV ATSPIQDAQ QQTSPQPPS SRHVRFLDI EGGGRHGGG QSEHESSES ETENTVMED EAVTSEKFL LLIDQLSVD QKRHLSIKD IGIILERLS SKILDVERL DRESESDDC YNWTIKATI RGDVLRELG VIYNGNYYA ISEHPGYKE ENEGVIEEA EEEEEEEDR L
- >TC011893 RA
atggcaaga gaggaatcc cgcggaaag agacccttc aagctatgg gacgggcgt cgtagtgtg cgcaagggg ctggtggtg gggagcctc gacgagcta gtgcagcgc ggaagagac aagctcggc gtctccatc ggagagccg gtgcgattg gtgttggag agcgacggt acccaggtc gaagacgcc gagtacttt cggacgcta cctgccaac accatcctt ctgctgctg aggccgggc gagcgatgg ctgcccgcc ggcgtggac gtcatccga gcagtagcc atctcagcg atcccgaag atcgtgtgc gaaacgatc cacgctttg gagctgcac gatgaaacg ccctcgtgg aagatcatg gacaacaag ggccgcgtc accgtcgtg cttcactgg gaccagcgt tcggccccg acgaggacc tcctcggag acgccgtcg cccgccccc ggcaagttc tcgccgagc aagcggccc tcgctcgtc atccagact tccgtgggg ggcaagaag gagactctc atcaatgat gtgtacgtg cctccaggc cagccccaa cctcgctac tccagccct cagatcact gtgataaac cacgacgac gttaaaccc gttcatcgg ctgtccaag cagggcagc tccttggag agcgccacc attcacatc catacgccc gagtgcgcc caccacgct cacatgccc cgcgccggt agcccccag agcaacggc gcgatcgag tgcgacttc cactgctgt gccctccac gaggagggc cggaagata gccgtgcac aagagcgtg gccacgtcg ccgattcaa gatgctcaa cagcagacc tcaccgcaa ccgccatca tctagacac gtgcgtttt ttagacatt gaaggtggc ggccgacac ggcggcggg cagtccgaa cacgagagc tccgaaagc gaaacggag aacaccgtg atggaggac gaggccgtc acctctgaa aaattcctg ctgttgatc gaccaattg agcgtcgat cagaaacga catctgagc atcaaagat atcgggatc atactcgaa aggctcagc tcgaaaatt ttggacgtt gaaaggtta gatcgcgag agcgaaagc gacgattgc tacaactgg accatcaaa gcgacgatt cgcggcgat gttctgcgc gaattgggc gtcatctac aacggcaac tattacgcc atttcggaa catccaggt tacaaggag gagaacgag ggggtcatt gaggaggca gaagaagaa gaagaggaa gaagacagg ctgtga
- >TC011893 RA
atcgatcgg acgctaggg ggcgcgttt gtcggggca gtttgcagg gttgctgct tgtaggggt cacagatag tccgattga attttggtc gagaatgga caaaaagag acggatcgg gctgagaat atgcggtgc cgtggttta gtgacagtg ccggtgcca tagaggagt gcgcgggcc agcggcggg acgacggcc gcaccataa gagatgccg tgatggcaa gagaggaat cccgcggaa agagaccct tcaagctat gggacgggc gtcgtagtg tgcgcaagg ggctggtgg tggggagcc tcgacgagc tagtgcagc gcggaagag acaagctcg gcgtctcca tcggagagc cggtgcgat tggtgttgg agagcgacg gtacccagg tcgaagacg ccgagtact ttcggacgc tacctgcca acaccatcc ttctgctgc tgaggccgg gcgagcgat ggctgcccg ccggcgtgg acgtcatcc gagcagtag ccatctcag cgatcccga agatcgtgt gcgaaacga tccacgctt tggagctgc acgatgaaa cgccctcgt ggaagatca tggacaaca agggccgcg tcaccgtcg tgcttcact gggaccagc gttcggccc cgacgagga cctcctcgg agacgccgt cgcccgccc ccggcaagt tctcgccga gcaagcggc cctcgctcg tcatccaga cttccgtgg ggggcaaga aggagactc tcatcaatg atgtgtacg tgcctccag gccagcccc aacctcgct actccagcc ctcagatca ctgtgataa accacgacg acgttaaac ccgttcatc ggctgtcca agcagggca gctccttgg agagcgcca ccattcaca tccatacgc ccgagtgcg cccaccacg ctcacatgc cccgcgccg gtagccccc agagcaacg gcgcgatcg agtgcgact tccactgct gtgccctcc acgaggagg gccggaaga tagccgtgc acaagagcg tggccacgt cgccgattc aagatgctc aacagcaga cctcaccgc aaccgccat catctagac acgtgcgtt ttttagaca ttgaaggtg gcggccgac acggcggcg ggcagtccg aacacgaga gctccgaaa gcgaaacgg agaacaccg tgatggagg acgaggccg tcacctctg aaaaattcc tgctgttga tcgaccaat tgagcgtcg atcagaaac gacatctga gcatcaaag atatcggga tcatactcg aaaggctca gctcgaaaa ttttggacg ttgaaaggt tagatcgcg agagcgaaa gcgacgatt gctacaact ggaccatca aagcgacga ttcgcggcg atgttctgc gcgaattgg gcgtcatct acaacggca actattacg ccatttcgg aacatccag gttacaagg aggagaacg agggggtca ttgaggagg cagaagaag aagaagagg aagaagaca ggctgtgaa ctgacagct aacgactgc caatgtcat aatgcacaa aagcagact ctagtaata agcacaggt gcaaaatat cacatatcg gatgtgaac tttgtaaac cgtactccg tactgactt gccatggtg atcgatcct tcttaaaac acgcgttgc gagttgtgc ggggcgacg actgccaca ccgtctcgt agtctaata aacaaaaaa tattgatac ctattggaa attaaatga gaaaaagat aaaaccaca aaaaaatca ttaaggaaa acataggaa aacaaaatt aacgacacg acttgacta gttgtatag ggttgacaa tcgattctc gatcggtgc gattcgatt cgttcgttt aaacgagtt tttaagtca ccgacgatg attacacac gaatactga tgcaaaaga acatgaaaa attgtgttt gtataggta tccttgtta atcatgtaa atcatttat aaataataa aactattcg cttagatgg ttgtttgtt gtgcgcgcg agtacgcga aacgcgtcg caggactct tctcatgat gactttcat tataacatt attacactc aactatcca ccgtggacg tggctttag ccgcacggg ggaccccta ggattatat ataaacgct ttgtacttt ttataatta tgtggattg tacgagtac ttagccaat ttaaagtta attattttt aagaaatta ttagcccag gtcatttag atgagacat gatgattca gtatttata taaaatata ttatataag tatataaga ggaagttgt ttaggatat ttgagatgt ttataactg ggttactcg atgattgat tgattgatt gattgtata ttgctcaat tgtcctttt gtttttgta atcattaac caccatcgg tcgggtatg ttgtgataa gatattgcc gagcgcgat atatattat atatattat tgtaattaa gtatatata atattgaag aaaaaagaa agctagggt tggggggtg aaacgggcg aatcaattt gggacactt ggtgatttt ttcatgaga atggaaagc aaacgtagg tctatatta gataaatta attataaag tgaataaaa ctgtgcaaa aaaattgaa aaaaaattt tttttggaa tttgatctg ggggtgcgt ttcggctcc gcggtccgt tcgcacgcc gcttgtctt gttgaggcg tgcctcgga gcggaatcg catcgatgg tcgatggac gcgtcccag caaacaaac agcaaagaa caaacgacg tcgagtcca tcctccgcc aaacatgtt ctaaaatct aattaccac tgccatcaa cctgtaaac cacgagaaa attgcaaaa ttccgggtg atcagctga cgggacggg acgggacga cgggcgggg cgccgcccc ccgccggag gcccccatc gatcgccgg tgcaccatc gggaccccg tcttggtgg tgcacggcc ccgaaaacg ggaactgca ctgcgaaac ccgagtcgg gggcgggcc gggagcggc cgggtcgag cggccgttg actcctgcg aggggagga cgcgctcct gtcacggca cactgccct acttccgag cggaggggc gaacctatc attcggcct ccggtgtta ttatgcaat acgtgataa ttctcatca cttccatca gacgagcca ccagctccg ggtacttcg ttcgtcgtg cgagtgaat tatttatta cggcgtcac taacacatt attcatcgc ggatttttc attcggttt tcgacgtat tattataat tgtcgttcg ttcgttcgt tcgttcgtt ccttcggtc atcatcaag caaaaaaag agcaaaaac gaaacgaaa aaactattc actcatcgt ttctacatt gttcatcat cattgtttt aaataggaa aattaatgt taattagat aaacaagaa agaactatt atttat
