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Gene information
General information
TC011870 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12364626..12368045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011870
    iBeetle screen iB_05120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011870 RA
      MAYLDQREI ANFLAQELF YQQFIALEG INSGVPTRT 
      TPTLTPTTL RSIEQTFIE LQTKPESHE NEAGFVPPL 
      VHSIGNNQY ISASDADIY SSKSQNMWQ TSLSQSSSD 
      SNTRSTPPL TQKTSLINN TPQTRRNMG GRKPARDLN 
      ISPEEEERR RIRRERNKA AAARCRKRR VDHTNALIK 
      ETEDLEQKK QALQEEIQE YQRMKDELD YLLESHRSS 
      SSNCRLHSQ SPPDVKPFV FGSRVNGDR VKPELDEAS 
      LPTSNDSFC IPSPAKRVM LSSTAPISK PCRPNSLNV 
      MSTAPKNNS ISDIAGIPI TTPSTGIQF NFDSLMVGG 
      TGLTPVSAP LVPSCSTQQ RNIPTSAAD MTSPDACGP 
      PKLVSL
    CDS sequence
    • >TC011870 RA
      atggcctat ttagaccaa agagaaatc gctaatttt 
      ctcgcgcag gagttattt taccagcag ttcatagct 
      ctagaaggc atcaattcg ggagtacca actcgaaca 
      acccctact ttaactccg acaactctt cgaagtatc 
      gagcaaacc tttatagaa ttgcagacg aagccggaa 
      tctcacgaa aacgaagcc ggtttcgtc ccacctcta 
      gttcattct ataggaaac aatcagtat atttcagcg 
      tcggatgct gatatttac tcatcaaag tcacaaaac 
      atgtggcaa acttcactt tctcaatct agttcggac 
      tcaaacacc aggtcgacg ccacccctg acccagaaa 
      actagtctt attaataat acaccgcaa acgaggcgt 
      aatatggga gggaggaaa cccgcaaga gatctcaac 
      atcagtccg gaagaagaa gaacgaaga cgcatacgg 
      agagaacga aataaagcc gcagcggca cgctgcagg 
      aaacgcaga gtcgaccac acaaacgct ttaatcaaa 
      gagacggaa gatttggag cagaagaag caagcgctc 
      caggaggag atccaggag taccaacgc atgaaagac 
      gaattggac tacctcctg gaatcgcac cgttcctcc 
      tctagcaat tgccgcctg cactcgcag agcccccct 
      gacgtcaag ccgttcgtc ttcggaagt cgagtgaac 
      ggcgaccgg gtcaagccg gagctggac gaggcgtcg 
      ctcccgacg tcaaacgac tccttctgt ataccatcg 
      ccagcgaag cgagtgatg ttgagttcg accgctccc 
      atatcgaag ccttgcaga ccgaattcg ctgaacgtg 
      atgtcgaca gcccccaaa aacaacagc attagcgac 
      atagcgggc attccgatc acgacgccc tcgaccggc 
      atccagttc aatttcgac tcgctgatg gtggggggc 
      acagggctg acgcccgtc tcggccccc ttggtgcct 
      tcgtgcagc acccagcag aggaacatc cccacgagt 
      gctgccgat atgacgtcg ccggacgcg tgcgggccc 
      ccgaaactt gttagttta taa
    mRNA sequence
    • >TC011870 RA
      ggtcaattt ttcgaacga acgattccg atagcaacg 
      atattcgaa tatcaataa ttttgattt tgttattac 
      atttttaac gattttcgt gtggtacct gcccgaaat 
      ggcctattt agaccaaag agaaatcgc taattttct 
      cgcgcagga gttatttta ccagcagtt catagctct 
      agaaggcat caattcggg agtaccaac tcgaacaac 
      ccctacttt aactccgac aactcttcg aagtatcga 
      gcaaacctt tatagaatt gcagacgaa gccggaatc 
      tcacgaaaa cgaagccgg tttcgtccc acctctagt 
      tcattctat aggaaacaa tcagtatat ttcagcgtc 
      ggatgctga tatttactc atcaaagtc acaaaacat 
      gtggcaaac ttcactttc tcaatctag ttcggactc 
      aaacaccag gtcgacgcc acccctgac ccagaaaac 
      tagtcttat taataatac accgcaaac gaggcgtaa 
      tatgggagg gaggaaacc cgcaagaga tctcaacat 
      cagtccgga agaagaaga acgaagacg catacggag 
      agaacgaaa taaagccgc agcggcacg ctgcaggaa 
      acgcagagt cgaccacac aaacgcttt aatcaaaga 
      gacggaaga tttggagca gaagaagca agcgctcca 
      ggaggagat ccaggagta ccaacgcat gaaagacga 
      attggacta cctcctgga atcgcaccg ttcctcctc 
      tagcaattg ccgcctgca ctcgcagag cccccctga 
      cgtcaagcc gttcgtctt cggaagtcg agtgaacgg 
      cgaccgggt caagccgga gctggacga ggcgtcgct 
      cccgacgtc aaacgactc cttctgtat accatcgcc 
      agcgaagcg agtgatgtt gagttcgac cgctcccat 
      atcgaagcc ttgcagacc gaattcgct gaacgtgat 
      gtcgacagc ccccaaaaa caacagcat tagcgacat 
      agcgggcat tccgatcac gacgccctc gaccggcat 
      ccagttcaa tttcgactc gctgatggt ggggggcac 
      agggctgac gcccgtctc ggccccctt ggtgccttc 
      gtgcagcac ccagcagag gaacatccc cacgagtgc 
      tgccgatat gacgtcgcc ggacgcgtg cgggccccc 
      gaaacttgt tagtttata aagaaaagc gctgtagcg 
      agatgtgtg ttatgtggg gaaaagttt catgttgtt 
      gtattattt tctaattaa actaagagt ttatttatt 
      atatttttg gactttttt tcacatacg catgtcgag 
      ctacgggct aggttagcc gagagatca atgttggac 
      gagctcatc tttagcgaa aaagtgaaa ttttttagg 
      gattttatg actgccgcc gcatgtcca acgtgttta 
      ccaaccatg tttagtatt cttgaaacc aaaccaaaa 
      atatttatt actaattaa tttattagt aatgttcta 
      gtgtcattt ttgcattat ttattgtta tgttttttt 
      aaatagtca tttgtatat aacgtgtgt actaaacat 
      ggaccaaaa atgtcgtca aagtgctca agctatttt 
      gctatgcta aatgcagat ttatttttt gctaccaag 
      aaatgcctt ttttgtaat ggtaaaaaa atctgtata 
      ttttcactt aacgcaaag ttattccct ccatacatt 
      tttatatga tagatttaa ggttaatgt tatattttt 
      gcaatagga gtgctccta ttccaataa gcgaatttg 
      tgttactac tattaagaa tataattaa taatatatt 
      gagaatact ttaacaaat aggactatc agcaatact 
      gtggttgca atatttgta atcactcgt caattttat 
      atagagttt gtcaaattt tgtatttat tttatgcga 
      tgccaatta taaaaaata aaatctatc ttttaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY