General information | |||
TC011861 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12438538..12440586 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC011861 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05117 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages partially orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
- >TC011861 RA
MCFSDNIKF AASAFNTIF ILIGGALLS IGIIYKLSF DQLFQVIPE AYNYLELTP ILTTIVGVA LLNAALFGF IIIYFERYH LKTTYCVVI LLIFTSQVC VGIFALVQT KDGDDLSKR IVTTTETLF NQTENGELI NAIQTSLKC CGPFGPTFW NETSNKPFP ESCCSDKTC TNVHPNGCR SSIFEFLLN SCKITGIAL LAFALIEMI GTVFMCHTF FRLNEASKM CLVQECVKF VVLAFNLVF VLVGAALIT LGSLYLANL NDITKAIPD PYDHFNLIP ILTIVIGSI IFLISFFGC CGACRNSPC LLSTYAVIL LIIFIAQIA IGVFAFLEI KNTDDLGKE VNKTIDEVF HKTSKADVE LINLIQQDI KCCGTDGPS FWATTPNKT IPSSCYEDQ KNLKGLFQE GCQGAFYNF LMDAARIIG IVVLVLAVT EVVGATFAL CLANSARNA LRRNDYY
- >TC011861 RA
atgtgtttt tcggacaat attaagttt gcggcatcg gcatttaac acaattttt atcctaatc ggaggagct ctcttatcg attggcatt atctataag ctaagcttt gatcagtta tttcaagtc attccggaa gcttacaat tacctagag cttacgccg attttgacc acaattgtg ggtgtcgcc cttttaaat gccgcttta ttcggcttc attatcatt tattttgaa cgctaccac cttaagaca acttactgt gtcgtaatt ttgctcatt ttcacgtcc caagtctgt gttggaatt ttcgccctc gtgcaaact aaagatggg gacgacctg tccaaacgt attgttacc acaacagag acacttttc aatcaaacg gaaaacgga gaactcatc aatgcaatc caaaccagc ttaaagtgt tgcggaccg tttggacca actttttgg aacgaaacc tcaaacaaa ccgttcccg gagtcctgc tgtagcgac aaaacctgc acaaacgtg caccccaac gggtgccga agctccatc ttcgaattt ctactaaac agttgcaaa ataaccggt attgcactt ctagctttc gcgctaatt gagatgatt ggaactgtc tttatgtgt catactttc tttcggttg aacgaagct tcgaaaatg tgtcttgtg caagaatgt gtcaagttt gtggtcctt gcgttcaat ctcgtattt gtgctcgtg ggggccgct cttatcacc ctaggcagc ctctacttg gccaactta aacgacatc actaaggcc atcccggac ccctacgac cacttcaat ctcatcccc atcctgaca atcgtcatc ggctctatc attttcctg atctcgttc ttcggctgc tgcggcgcc tgcaggaac agcccgtgt ctcctctcc acgtacgct gtgatcctc ctcatcatc ttcatagcc cagatagcg atcggagtg ttcgcgttt ttggaaatt aaaaacaca gacgacttg gggaaagaa gttaataag actatagat gaagtgttc cacaagacg tccaaagcc gatgttgaa cttatcaac ttgatccag caagatata aagtgttgt gggaccgat gggccaagt ttttgggcc actacgcca aataaaacc atcccgtct tcctgttac gaggaccaa aaaaaccta aagggtttg ttccaggaa gggtgccaa ggagcgttc tataatttc ctgatggat gctgccagg attatcggg attgttgtt ttggtcttg gcggtcact gaagttgtg ggggccacg tttgccttg tgtttggcg aactcagcg cgaaacgcc ctccgaagg aatgattac tattag
- >TC011861 RA
aggaggaga taaagaaaa tattggcta aaatgtaga ataattcaa actgcgatg tgtttttcg gacaatatt aagtttgcg gcatcggca tttaacaca atttttatc ctaatcgga ggagctctc ttatcgatt ggcattatc tataagcta agctttgat cagttattt caagtcatt ccggaagct tacaattac ctagagctt acgccgatt ttgaccaca attgtgggt gtcgccctt ttaaatgcc gctttattc ggcttcatt atcatttat tttgaacgc taccacctt aagacaact tactgtgtc gtaattttg ctcattttc acgtcccaa gtctgtgtt ggaattttc gccctcgtg caaactaaa gatggggac gacctgtcc aaacgtatt gttaccaca acagagaca cttttcaat caaacggaa aacggagaa ctcatcaat gcaatccaa accagctta aagtgttgc ggaccgttt ggaccaact ttttggaac gaaacctca aacaaaccg ttcccggag tcctgctgt agcgacaaa acctgcaca aacgtgcac cccaacggg tgccgaagc tccatcttc gaatttcta ctaaacagt tgcaaaata accggtatt gcacttcta gctttcgcg ctaattgag atgattgga actgtcttt atgtgtcat actttcttt cggttgaac gaagcttcg aaaatgtgt cttgtgcaa gaatgtgtc aagtttgtg gtccttgcg ttcaatctc gtatttgtg ctcgtgggg gccgctctt atcacccta ggcagcctc tacttggcc aacttaaac gacatcact aaggccatc ccggacccc tacgaccac ttcaatctc atccccatc ctgacaatc gtcatcggc tctatcatt ttcctgatc tcgttcttc ggctgctgc ggcgcctgc aggaacagc ccgtgtctc ctctccacg tacgctgtg atcctcctc atcatcttc atagcccag atagcgatc ggagtgttc gcgtttttg gaaattaaa aacacagac gacttgggg aaagaagtt aataagact atagatgaa gtgttccac aagacgtcc aaagccgat gttgaactt atcaacttg atccagcaa gatataaag tgttgtggg accgatggg ccaagtttt tgggccact acgccaaat aaaaccatc ccgtcttcc tgttacgag gaccaaaaa aacctaaag ggtttgttc caggaaggg tgccaagga gcgttctat aatttcctg atggatgct gccaggatt atcgggatt gttgttttg gtcttggcg gtcactgaa gttgtgggg gccacgttt gccttgtgt ttggcgaac tcagcgcga aacgccctc cgaaggaat gattactat tagacatag aagtatttt tccaaaatt attcttgtg tgtattcct gtctgccta aactagact gtttacaac ttatattat ataataaga atatacgag tactttaca tgaaccttt gcacggagc aaaaacttg tgtgaaatg attttcaat aaacgattt attctgtg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
