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Gene information
General information
TC011861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12438538..12440586
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011861
    iBeetle screen iB_05117

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages partially orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011861 RA
      MCFSDNIKF AASAFNTIF ILIGGALLS IGIIYKLSF 
      DQLFQVIPE AYNYLELTP ILTTIVGVA LLNAALFGF 
      IIIYFERYH LKTTYCVVI LLIFTSQVC VGIFALVQT 
      KDGDDLSKR IVTTTETLF NQTENGELI NAIQTSLKC 
      CGPFGPTFW NETSNKPFP ESCCSDKTC TNVHPNGCR 
      SSIFEFLLN SCKITGIAL LAFALIEMI GTVFMCHTF 
      FRLNEASKM CLVQECVKF VVLAFNLVF VLVGAALIT 
      LGSLYLANL NDITKAIPD PYDHFNLIP ILTIVIGSI 
      IFLISFFGC CGACRNSPC LLSTYAVIL LIIFIAQIA 
      IGVFAFLEI KNTDDLGKE VNKTIDEVF HKTSKADVE 
      LINLIQQDI KCCGTDGPS FWATTPNKT IPSSCYEDQ 
      KNLKGLFQE GCQGAFYNF LMDAARIIG IVVLVLAVT 
      EVVGATFAL CLANSARNA LRRNDYY
    CDS sequence
    • >TC011861 RA
      atgtgtttt tcggacaat attaagttt gcggcatcg 
      gcatttaac acaattttt atcctaatc ggaggagct 
      ctcttatcg attggcatt atctataag ctaagcttt 
      gatcagtta tttcaagtc attccggaa gcttacaat 
      tacctagag cttacgccg attttgacc acaattgtg 
      ggtgtcgcc cttttaaat gccgcttta ttcggcttc 
      attatcatt tattttgaa cgctaccac cttaagaca 
      acttactgt gtcgtaatt ttgctcatt ttcacgtcc 
      caagtctgt gttggaatt ttcgccctc gtgcaaact 
      aaagatggg gacgacctg tccaaacgt attgttacc 
      acaacagag acacttttc aatcaaacg gaaaacgga 
      gaactcatc aatgcaatc caaaccagc ttaaagtgt 
      tgcggaccg tttggacca actttttgg aacgaaacc 
      tcaaacaaa ccgttcccg gagtcctgc tgtagcgac 
      aaaacctgc acaaacgtg caccccaac gggtgccga 
      agctccatc ttcgaattt ctactaaac agttgcaaa 
      ataaccggt attgcactt ctagctttc gcgctaatt 
      gagatgatt ggaactgtc tttatgtgt catactttc 
      tttcggttg aacgaagct tcgaaaatg tgtcttgtg 
      caagaatgt gtcaagttt gtggtcctt gcgttcaat 
      ctcgtattt gtgctcgtg ggggccgct cttatcacc 
      ctaggcagc ctctacttg gccaactta aacgacatc 
      actaaggcc atcccggac ccctacgac cacttcaat 
      ctcatcccc atcctgaca atcgtcatc ggctctatc 
      attttcctg atctcgttc ttcggctgc tgcggcgcc 
      tgcaggaac agcccgtgt ctcctctcc acgtacgct 
      gtgatcctc ctcatcatc ttcatagcc cagatagcg 
      atcggagtg ttcgcgttt ttggaaatt aaaaacaca 
      gacgacttg gggaaagaa gttaataag actatagat 
      gaagtgttc cacaagacg tccaaagcc gatgttgaa 
      cttatcaac ttgatccag caagatata aagtgttgt 
      gggaccgat gggccaagt ttttgggcc actacgcca 
      aataaaacc atcccgtct tcctgttac gaggaccaa 
      aaaaaccta aagggtttg ttccaggaa gggtgccaa 
      ggagcgttc tataatttc ctgatggat gctgccagg 
      attatcggg attgttgtt ttggtcttg gcggtcact 
      gaagttgtg ggggccacg tttgccttg tgtttggcg 
      aactcagcg cgaaacgcc ctccgaagg aatgattac 
      tattag
    mRNA sequence
    • >TC011861 RA
      aggaggaga taaagaaaa tattggcta aaatgtaga 
      ataattcaa actgcgatg tgtttttcg gacaatatt 
      aagtttgcg gcatcggca tttaacaca atttttatc 
      ctaatcgga ggagctctc ttatcgatt ggcattatc 
      tataagcta agctttgat cagttattt caagtcatt 
      ccggaagct tacaattac ctagagctt acgccgatt 
      ttgaccaca attgtgggt gtcgccctt ttaaatgcc 
      gctttattc ggcttcatt atcatttat tttgaacgc 
      taccacctt aagacaact tactgtgtc gtaattttg 
      ctcattttc acgtcccaa gtctgtgtt ggaattttc 
      gccctcgtg caaactaaa gatggggac gacctgtcc 
      aaacgtatt gttaccaca acagagaca cttttcaat 
      caaacggaa aacggagaa ctcatcaat gcaatccaa 
      accagctta aagtgttgc ggaccgttt ggaccaact 
      ttttggaac gaaacctca aacaaaccg ttcccggag 
      tcctgctgt agcgacaaa acctgcaca aacgtgcac 
      cccaacggg tgccgaagc tccatcttc gaatttcta 
      ctaaacagt tgcaaaata accggtatt gcacttcta 
      gctttcgcg ctaattgag atgattgga actgtcttt 
      atgtgtcat actttcttt cggttgaac gaagcttcg 
      aaaatgtgt cttgtgcaa gaatgtgtc aagtttgtg 
      gtccttgcg ttcaatctc gtatttgtg ctcgtgggg 
      gccgctctt atcacccta ggcagcctc tacttggcc 
      aacttaaac gacatcact aaggccatc ccggacccc 
      tacgaccac ttcaatctc atccccatc ctgacaatc 
      gtcatcggc tctatcatt ttcctgatc tcgttcttc 
      ggctgctgc ggcgcctgc aggaacagc ccgtgtctc 
      ctctccacg tacgctgtg atcctcctc atcatcttc 
      atagcccag atagcgatc ggagtgttc gcgtttttg 
      gaaattaaa aacacagac gacttgggg aaagaagtt 
      aataagact atagatgaa gtgttccac aagacgtcc 
      aaagccgat gttgaactt atcaacttg atccagcaa 
      gatataaag tgttgtggg accgatggg ccaagtttt 
      tgggccact acgccaaat aaaaccatc ccgtcttcc 
      tgttacgag gaccaaaaa aacctaaag ggtttgttc 
      caggaaggg tgccaagga gcgttctat aatttcctg 
      atggatgct gccaggatt atcgggatt gttgttttg 
      gtcttggcg gtcactgaa gttgtgggg gccacgttt 
      gccttgtgt ttggcgaac tcagcgcga aacgccctc 
      cgaaggaat gattactat tagacatag aagtatttt 
      tccaaaatt attcttgtg tgtattcct gtctgccta 
      aactagact gtttacaac ttatattat ataataaga 
      atatacgag tactttaca tgaaccttt gcacggagc 
      aaaaacttg tgtgaaatg attttcaat aaacgattt 
      attctgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY