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Gene information
General information
TC011859 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12475530..12477335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011859
    iBeetle screen iB_05116

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011859 RA
      MPYPESPFG DDFDAGILV RRADDDETN VSEIQIGEV 
      TKWLCQAPK ITSFRVNTL KADVGEAHR WIRAHIAGA 
      GLDLRVERH PSCADAVIV HHPVSHQTP QKHNKEIVV 
      DAVCGAAVL RGAHIYAPG VIGMMSNIQ IGDNVSIYV 
      DLDKKCKKG FLKLYNGKK TFIGNGEVI LGRNHLFCE 
      TPQTGLAIK MIETISSCP PVLGDNFLP PGAALLQNL 
      PSIICTHVL APCPNDTIL DMCASPGNK TTHIAALLK 
      NQGRLVAID KTPTKVAQL RQRCENFGA KVEILQTDS 
      TKLLDRFCY ESFDKILLD APCSALGKR PQFVNTTSR 
      KILRSYVPL QRKLFETAV QLLKTDGTL VYSTCTITL 
      AENEGIVAW ALKNFKLEL VEAAPILGG PGWDGTQLT 
      SEQLKKVQR FGPHQSIDS VGFFIAKFV KK
    CDS sequence
    • >TC011859 RA
      atgccatac ccagaatca cccttcggg gacgacttc 
      gatgcggga atcctggtc cggagggcc gatgacgac 
      gaaacaaac gtcagtgag attcaaatc ggggaggtc 
      acgaaatgg ctctgccaa gcgcccaaa ataacttct 
      tttagggta aacacgctc aaggccgac gtgggagag 
      gcacacaga tggatccgg gcacatatt gcaggtgca 
      gggctggac ctccgcgtg gagagacac cccagctgc 
      gcggacgcc gtcattgtc caccaccct gtatcacac 
      caaactccc caaaaacac aacaaagaa attgtcgtt 
      gatgcggtc tgtggggcc gctgtcttg cggggggcc 
      cacatttac gcccccggg gtcatagga atgatgtca 
      aacatacaa attggtgat aacgtgagc atttacgtc 
      gatctcgac aagaaatgt aaaaaaggc ttcctcaaa 
      ttatacaac ggcaagaaa acgttcatc ggaaacggt 
      gaagtaatc ctgggacgt aatcaccta ttttgcgaa 
      acacctcaa accggcctc gccataaaa atgattgaa 
      acaatatca agctgtccc cccgtgtta ggggacaat 
      tttttaccc ccaggtgcg gccctgcta caaaatttg 
      ccctccata atttgcaca cacgtgctt gctccgtgc 
      cccaatgac actattttg gacatgtgt gcttccccg 
      gggaacaaa acaacgcat attgcggcc cttttgaaa 
      aaccaaggc agattggtc gcaattgac aaaaccccg 
      actaaagtt gcacagttg cgacaacgt tgcgaaaat 
      ttcggggct aaagttgaa attttacaa accgattcg 
      accaaatta ctcgatcgt ttctgttat gaatctttt 
      gataaaatt ttattagac gccccttgt agtgctttg 
      ggcaaaaga ccgcaattt gttaataca acgtcgcgg 
      aaaattctg cgatcgtat gtcccccta caacggaaa 
      ttattcgaa acggccgtc caacttttg aaaacggac 
      ggaactctg gtttacagc acttgtacg atcactttg 
      gctgagaac gaggggatt gtggcttgg gctttgaaa 
      aacttcaaa ctcgaattg gtggaagcg gccccaatt 
      ctgggcggc cctggatgg gacgggaca cagttgact 
      agcgaacaa ctgaaaaaa gtgcagcgt ttcgggcca 
      catcaaagc atagactct gtcggtttt tttattgca 
      aaatttgtg aaaaaataa
    mRNA sequence
    • >TC011859 RA
      ccccgcccc acgtgaccc acaattgcc aatcaaaac 
      aagctaacc tagtgcagg tgcaaagcc gaaattccg 
      ccgaaccgg gcggttttc taagtgcac cgactcctg 
      gttcaccac acgcgcaat gccataccc agaatcacc 
      cttcgggga cgacttcga tgcgggaat cctggtccg 
      gagggccga tgacgacga aacaaacgt cagtgagat 
      tcaaatcgg ggaggtcac gaaatggct ctgccaagc 
      gcccaaaat aacttcttt tagggtaaa cacgctcaa 
      ggccgacgt gggagaggc acacagatg gatccgggc 
      acatattgc aggtgcagg gctggacct ccgcgtgga 
      gagacaccc cagctgcgc ggacgccgt cattgtcca 
      ccaccctgt atcacacca aactcccca aaaacacaa 
      caaagaaat tgtcgttga tgcggtctg tggggccgc 
      tgtcttgcg gggggccca catttacgc ccccggggt 
      cataggaat gatgtcaaa catacaaat tggtgataa 
      cgtgagcat ttacgtcga tctcgacaa gaaatgtaa 
      aaaaggctt cctcaaatt atacaacgg caagaaaac 
      gttcatcgg aaacggtga agtaatcct gggacgtaa 
      tcacctatt ttgcgaaac acctcaaac cggcctcgc 
      cataaaaat gattgaaac aatatcaag ctgtccccc 
      cgtgttagg ggacaattt tttaccccc aggtgcggc 
      cctgctaca aaatttgcc ctccataat ttgcacaca 
      cgtgcttgc tccgtgccc caatgacac tattttgga 
      catgtgtgc ttccccggg gaacaaaac aacgcatat 
      tgcggccct tttgaaaaa ccaaggcag attggtcgc 
      aattgacaa aaccccgac taaagttgc acagttgcg 
      acaacgttg cgaaaattt cggggctaa agttgaaat 
      tttacaaac cgattcgac caaattact cgatcgttt 
      ctgttatga atcttttga taaaatttt attagacgc 
      cccttgtag tgctttggg caaaagacc gcaatttgt 
      taatacaac gtcgcggaa aattctgcg atcgtatgt 
      ccccctaca acggaaatt attcgaaac ggccgtcca 
      acttttgaa aacggacgg aactctggt ttacagcac 
      ttgtacgat cactttggc tgagaacga ggggattgt 
      ggcttgggc tttgaaaaa cttcaaact cgaattggt 
      ggaagcggc cccaattct gggcggccc tggatggga 
      cgggacaca gttgactag cgaacaact gaaaaaagt 
      gcagcgttt cgggccaca tcaaagcat agactctgt 
      cggtttttt tattgcaaa atttgtgaa aaaataaat 
      taatccatg ttgaatttc tcgttattt gagccgtga 
      ccagtcacg ggtacatca ataaaatat tgaggaatg 
      t
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