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Gene information
General information
TC011848 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12597915..12605792
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011848
    iBeetle screen iB_05115

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011848 RA
      MFVFVIFAL NWLTIRADL WSSNNPTVF NIGGVLSSN 
      ESEYYFKET IAHLNFDSQ YVPKGVTYY DTAILMDPN 
      PIKTALNVC KYLITSRVY AVVVSHPLT GDLSPAAVS 
      YTSGFYHIP VIGISSRDS AFSDKNIHV SFLRTVPPY 
      SHQADVWVE MLKHFNYKK VIFIHSSDT DGRALLGRF 
      QTTSQSLED DVEIKVQVE SIIEFEPGL ETFKEQLSD 
      MKNAQSRVY LMYASKTDA QVIFRDAAE FNMTDAGYA 
      WIVTEQALV ANNIPEGIL GLRLVNATN EKAHIKDSI 
      YVLASALRD LNQTKEITE APKDCDDSG QIWETGRDL 
      FDFIKKQVL MNGETGKVA FDDQGDRIN AEYNIVNIQ 
      RKRKQVTVG KFFFNRTSN KMRLAVDEN NILWPGRQH 
      VKPEGFMIP THLKVLTIE EKPFVYVRK LVEPQDVCT 
      AEEIPCPHF NATQDLAGS YCCKGYCMD LLKELSKKI 
      NFTYSLALS PDGQFGNYI IRNSSGSGK KEWTGLIGE 
      LVGERADMI VAPLTINPE RAEFIEFSK PFKYQGITI 
      LEKKPSRSS TLVSFLQPF SNTLWILVM VSVHVVALV 
      LYLLDRFSP FGRFKLANT DGTEEDALN LSSAIWFAW 
      GVLLNSGIG EGTPRSFSA RVLGMVWAG FAMIIVASY 
      TANLAAFLV LERPKTKLT GINDARLRN TMENLTCAT 
      VKGSAVDMY FRRQVELSN MYRTMEANN YNTAEDAIE 
      DVKVGKLMA FIWDSSRLE FEAAQDCEL VTAGELFGR 
      SGYGIGLQK GSPWADDIT LAILDFHES GFMESLDNK 
      WILQGNVQQ CEQFEKTPN TLGLKNMAG VFILVAAGI 
      VGGIGLIVI EMAYKKHQI KKQKRMELA RHAADKWRG 
      CVEKRKTLR ASATTQRRI KSNGVNDPA TISLAVDKY 
      QRIGGPERA WPGDSDIRQ RRVEDSGGV QPVPRYLPA 
      YTSDVSHLI V
    CDS sequence
    • >TC011848 RA
      atgtttgtt ttcgtgatt tttgcacta aattggctc 
      acaattcgg gccgacttg tggtcgtcc aacaacccc 
      acggttttc aacatcggg ggcgtgtta agcagcaat 
      gagagcgag tactacttc aaggaaaca atcgcgcac 
      ttgaatttc gactcccag tatgtgccc aaaggggtc 
      acttattac gacactgca attctcatg gaccccaac 
      cccatcaaa accgccctc aacgtttgc aaatacttg 
      atcacaagt cgggtgtat gccgtggtg gtttcgcac 
      cccttgacc ggggactta tccccagca gcggtgtcg 
      tacactagt ggtttctac cacatcccg gtgattggt 
      atctcaagc cgggattcg gctttttcg gacaaaaac 
      attcatgtc tcgtttctg cgaactgtg cccccgtac 
      tcccaccag gccgacgtt tgggtcgaa atgctcaag 
      cacttcaac tacaagaag gtgattttc atccacagt 
      tcggacact gatgggcgt gccctgctg gggaggttc 
      cagaccacc tcccagagc ctggaggac gatgtggag 
      atcaaggtc caggtcgag tcgattata gagtttgag 
      ccgggtttg gaaacgttc aaggagcaa ctcagcgac 
      atgaaaaac gcccagtct agagtctat ctgatgtat 
      gccagcaaa accgacgct caggtcatt ttccgagac 
      gctgctgaa tttaatatg acggatgct gggtatgcg 
      tggatcgtc acagagcaa gcccttgtt gccaataac 
      atccccgag gggattctg ggcctccga ctggtcaat 
      gcgaccaac gaaaaagcc cacatcaag gacagcatt 
      tatgtcctg gcgtcggct ttacgcgac ctaaaccaa 
      acgaaggaa atcaccgaa gcgccaaaa gactgcgac 
      gattcgggc cagatttgg gaaacaggc cgcgacctt 
      ttcgacttc atcaaaaag caagtccta atgaacggt 
      gagaccgga aaagtggct ttcgacgac caaggcgac 
      cgaatcaac gccgaatac aacatcgtc aacatccaa 
      agaaaacgc aagcaagtc acagtcggg aaatttttc 
      ttcaacagg accagcaat aaaatgcgt ttggcggtc 
      gacgagaac aacattttg tggccgggt cgccagcac 
      gtcaagccc gagggtttc atgatcccg acccacctc 
      aaggtcctc acaattgag gaaaaaccg ttcgtttac 
      gtccggaag ctggtggaa ccccaggac gtgtgcacc 
      gctgaggaa ataccttgt cctcacttc aacgccacg 
      caagatctg gcggggagt tactgttgc aagggttat 
      tgcatggac ctcctgaag gagttgtcg aagaaaatc 
      aacttcact tatagtctg gcgctgtct cccgacggc 
      cagttcggc aactacatc atcaggaac agctccggg 
      agtggcaag aaggagtgg actggcttg ataggggag 
      ctggtgggc gagagggcc gacatgata gtcgccccc 
      ttgaccatc aaccccgag cgggccgaa ttcatcgag 
      ttcagcaag ccgttcaaa taccaaggc atcacaatt 
      ctggagaaa aaaccgtcc cggtcgtcc acacttgtg 
      tccttcttg cagccgttc agcaacact ctctggatc 
      ttggtgatg gtgtccgtg cacgtggtg gccctcgtg 
      ttgtacctc ctggaccgg ttttcgccc tttgggcgc 
      ttcaagctg gcaaatacc gacgggacg gaggaagac 
      gccctgaat ctctcaagc gcgatttgg ttcgcttgg 
      ggagtgctg ctcaacagt gggattggg gaagggact 
      ccgaggagc ttctcggcc agggttctg ggcatggtt 
      tgggccggt tttgcgatg attatcgta gcttcgtat 
      actgccaac ttggccgcg tttctcgtt ttggaaaga 
      cccaaaact aaactgact gggattaat gatgccagg 
      ctgaggaac acgatggag aatttgact tgtgcgacg 
      gtcaagggg tctgcggtt gatatgtat ttccgcaga 
      caggtcgaa ttgtcgaat atgtacagg accatggaa 
      gcgaataat tataacacg gccgaggac gctattgaa 
      gatgtcaaa gtgggaaag ctgatggcc ttcatctgg 
      gacagctct cgcctggag ttcgaggcg gcccaagac 
      tgcgaactc gtgaccgcc ggcgagctc ttcggccgc 
      tccggctac ggcataggc ctccagaag ggttcgccc 
      tgggccgac gacatcacc ctcgcgatt ctggacttc 
      catgaaagc ggcttcatg gagagcctg gacaacaag 
      tggatcctc caaggcaac gtccagcaa tgcgagcag 
      ttcgaaaaa acgcccaac accctcggc ttgaagaac 
      atggccggg gtgttcatc ctcgtggcc gccgggatc 
      gtgggcggc atcggcctg atcgtcatt gaaatggca 
      tacaagaag caccaaatc aagaaacag aaacgcatg 
      gagctggcc agacatgcg gcggataag tggcgcggg 
      tgtgtcgag aagcgcaag accttgcgg gcctccgcc 
      accacgcaa cgccggatc aagtccaat ggggtgaac 
      gatccggcg accatttca ctggcggtt gacaagtac 
      cagaggatt ggggggccc gagagggcc tggccgggg 
      gattccgac ataaggcaa aggagggtg gaggacagt 
      gggggcgtg caaccagtt cctagatat ctaccggcg 
      tacactagc gatgtatcg catctcatt gtttaa
    mRNA sequence
    • >TC011848 RA
      aaaatttgt aagtggcgc ccaacacgt gaccgaagc 
      cccgcatca aatatgata aaataacaa ttgaaccac 
      gatttgttt cttttgttt tcaaggggg gtacctatg 
      atgagttaa ggcaggtca tttgaaatc gatatcatc 
      tggcgtcgt cttggcaac gggcgcggt ccagcttca 
      cacgagatg aaactctgc gtttaaaca atgtttgtt 
      ttcgtgatt tttgcacta aattggctc acaattcgg 
      gccgacttg tggtcgtcc aacaacccc acggttttc 
      aacatcggg ggcgtgtta agcagcaat gagagcgag 
      tactacttc aaggaaaca atcgcgcac ttgaatttc 
      gactcccag tatgtgccc aaaggggtc acttattac 
      gacactgca attctcatg gaccccaac cccatcaaa 
      accgccctc aacgtttgc aaatacttg atcacaagt 
      cgggtgtat gccgtggtg gtttcgcac cccttgacc 
      ggggactta tccccagca gcggtgtcg tacactagt 
      ggtttctac cacatcccg gtgattggt atctcaagc 
      cgggattcg gctttttcg gacaaaaac attcatgtc 
      tcgtttctg cgaactgtg cccccgtac tcccaccag 
      gccgacgtt tgggtcgaa atgctcaag cacttcaac 
      tacaagaag gtgattttc atccacagt tcggacact 
      gatgggcgt gccctgctg gggaggttc cagaccacc 
      tcccagagc ctggaggac gatgtggag atcaaggtc 
      caggtcgag tcgattata gagtttgag ccgggtttg 
      gaaacgttc aaggagcaa ctcagcgac atgaaaaac 
      gcccagtct agagtctat ctgatgtat gccagcaaa 
      accgacgct caggtcatt ttccgagac gctgctgaa 
      tttaatatg acggatgct gggtatgcg tggatcgtc 
      acagagcaa gcccttgtt gccaataac atccccgag 
      gggattctg ggcctccga ctggtcaat gcgaccaac 
      gaaaaagcc cacatcaag gacagcatt tatgtcctg 
      gcgtcggct ttacgcgac ctaaaccaa acgaaggaa 
      atcaccgaa gcgccaaaa gactgcgac gattcgggc 
      cagatttgg gaaacaggc cgcgacctt ttcgacttc 
      atcaaaaag caagtccta atgaacggt gagaccgga 
      aaagtggct ttcgacgac caaggcgac cgaatcaac 
      gccgaatac aacatcgtc aacatccaa agaaaacgc 
      aagcaagtc acagtcggg aaatttttc ttcaacagg 
      accagcaat aaaatgcgt ttggcggtc gacgagaac 
      aacattttg tggccgggt cgccagcac gtcaagccc 
      gagggtttc atgatcccg acccacctc aaggtcctc 
      acaattgag gaaaaaccg ttcgtttac gtccggaag 
      ctggtggaa ccccaggac gtgtgcacc gctgaggaa 
      ataccttgt cctcacttc aacgccacg caagatctg 
      gcggggagt tactgttgc aagggttat tgcatggac 
      ctcctgaag gagttgtcg aagaaaatc aacttcact 
      tatagtctg gcgctgtct cccgacggc cagttcggc 
      aactacatc atcaggaac agctccggg agtggcaag 
      aaggagtgg actggcttg ataggggag ctggtgggc 
      gagagggcc gacatgata gtcgccccc ttgaccatc 
      aaccccgag cgggccgaa ttcatcgag ttcagcaag 
      ccgttcaaa taccaaggc atcacaatt ctggagaaa 
      aaaccgtcc cggtcgtcc acacttgtg tccttcttg 
      cagccgttc agcaacact ctctggatc ttggtgatg 
      gtgtccgtg cacgtggtg gccctcgtg ttgtacctc 
      ctggaccgg ttttcgccc tttgggcgc ttcaagctg 
      gcaaatacc gacgggacg gaggaagac gccctgaat 
      ctctcaagc gcgatttgg ttcgcttgg ggagtgctg 
      ctcaacagt gggattggg gaagggact ccgaggagc 
      ttctcggcc agggttctg ggcatggtt tgggccggt 
      tttgcgatg attatcgta gcttcgtat actgccaac 
      ttggccgcg tttctcgtt ttggaaaga cccaaaact 
      aaactgact gggattaat gatgccagg ctgaggaac 
      acgatggag aatttgact tgtgcgacg gtcaagggg 
      tctgcggtt gatatgtat ttccgcaga caggtcgaa 
      ttgtcgaat atgtacagg accatggaa gcgaataat 
      tataacacg gccgaggac gctattgaa gatgtcaaa 
      gtgggaaag ctgatggcc ttcatctgg gacagctct 
      cgcctggag ttcgaggcg gcccaagac tgcgaactc 
      gtgaccgcc ggcgagctc ttcggccgc tccggctac 
      ggcataggc ctccagaag ggttcgccc tgggccgac 
      gacatcacc ctcgcgatt ctggacttc catgaaagc 
      ggcttcatg gagagcctg gacaacaag tggatcctc 
      caaggcaac gtccagcaa tgcgagcag ttcgaaaaa 
      acgcccaac accctcggc ttgaagaac atggccggg 
      gtgttcatc ctcgtggcc gccgggatc gtgggcggc 
      atcggcctg atcgtcatt gaaatggca tacaagaag 
      caccaaatc aagaaacag aaacgcatg gagctggcc 
      agacatgcg gcggataag tggcgcggg tgtgtcgag 
      aagcgcaag accttgcgg gcctccgcc accacgcaa 
      cgccggatc aagtccaat ggggtgaac gatccggcg 
      accatttca ctggcggtt gacaagtac cagaggatt 
      ggggggccc gagagggcc tggccgggg gattccgac 
      ataaggcaa aggagggtg gaggacagt gggggcgtg 
      caaccagtt cctagatat ctaccggcg tacactagc 
      gatgtatcg catctcatt gtttaactc gcttaatag 
      ttgttggtt attggatgt agtcattta tcagatgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY