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Gene information
General information
TC011844 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12657461..12659854
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011844
    iBeetle screen iB_05112

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011844 RA
      MASAYKLSE GALQTIMYG GEVIDPLVQ ILGTKKMSP 
      GGSDSSSRL RLMISDGLH KVNIAVFTV PKNDQTLPH 
      VLEQVFTII KLKRYLSNV IKNKNNIDA RVIAILELE 
      VVARGEFVG QIIGNPVSL SDSETLTQS SDVIKRNEC 
      INGSFSSKT ISPIASLNP FHNNWIIRA RVVNKTNVR 
      NWSNAKGEG KLFSIDLVD KGGEIRCTA FRDLVDKFF 
      DRLEVDKVY YISKCQLQP INRQFCSLK HQFEMYFTP 
      ETIIEPSYD DNEIIPQIT YNFTTIDKV AVMEAGTIV 
      DVIGVCKTI SELQSFVAK STNREMQKR QLVLVDQTK 
      TAISLNLWG SQAESFAVT GNPVILVKN AKIYEFRGA 
      KSITLMGTS LLKIDPEIR ETHILRRWY HSEGNQVEI 
      TNVSHRNGY GSTPWLTFK EARQQTISE KPLYYQTFG 
      TILLVRLDR AVYKSCPNA DCQKKVLDL ENGMYRCEK 
      CNREFPNFK YRLLVSMNV ADSSSSQWV TVFSSEAEK 
      ILGKTAQEI GELMETDPD TVANMFEEA QFKQFIFKC 
      RAKIETYND EQKMKTVVV TVAPVDYEE YNAYLVDRI 
      EKFTGVPCN
    CDS sequence
    • >TC011844 RA
      atggcttct gcgtacaaa ttatcagaa ggggcgctt 
      cagacgatt atgtacggg ggcgaggtc attgaccct 
      cttgttcaa attttgggg actaaaaaa atgtctcca 
      gggggtagc gacagcagc tcgcgctta cgcctgatg 
      atttcggac ggtttgcac aaagtaaat attgcagtg 
      tttactgtg ccaaagaac gaccagact ttgccccac 
      gtcttagag caggtgttt acgattatt aaactaaag 
      cgctatctt tccaacgtt ataaaaaac aagaataac 
      atagatgcg cgagttatt gcgattttg gaactggaa 
      gtggttgcg cgtggggag tttgtaggt cagataatc 
      ggaaaccct gtctcttta tccgactct gaaaccttg 
      acgcaatcg agtgacgtt atcaagaga aatgagtgc 
      ataaatggc tcattttcc tcaaaaaca atcagtccc 
      atagctagc ttaaacccc tttcacaac aactggata 
      attagggcg agagtcgtt aataagacg aatgtgaga 
      aactggagc aatgccaag ggggagggt aagcttttc 
      agcatcgat ttggtcgac aaagggggc gaaatcaga 
      tgcaccgct ttccgggac cttgttgat aagtttttc 
      gatcgcctt gaggtcgac aaggtttac tacattagc 
      aaatgccag ctccagcca atcaatcgc caattttgc 
      tccttaaag caccagttt gaaatgtac ttcaccccg 
      gaaacgatc atcgagccg agttatgac gacaatgag 
      atcatccca caaataacg tacaatttc accacgatt 
      gataaagta gcggtcatg gaagctggg accattgtt 
      gacgtgatc ggcgtttgc aagacaatt tcggaactg 
      cagagtttt gtggcgaaa tcaaccaat cgcgagatg 
      cagaaaaga cagctcgtg ttagtcgat cagaccaaa 
      accgcgatt tctctgaat ttgtggggc tcacaggca 
      gagtcgttc gcagtcacc ggtaatccg gtgattttg 
      gttaaaaat gcaaagatt tatgaattt agaggtgcg 
      aaaagtatc actttgatg ggcactagt ttgcttaaa 
      attgaccca gaaatacgg gaaacccac attttgagg 
      cggtggtac cattcggaa gggaatcag gtggaaata 
      acgaatgtc agtcatagg aacggatac ggatctacg 
      ccatggctg acgttcaaa gaagcgcgc caacagacc 
      atttccgaa aaaccgctt tactaccaa actttcggg 
      acgattttg ttggttcgc ctcgacaga gcggtgtac 
      aagtcgtgt ccgaacgct gattgccag aagaaggtc 
      ctcgatttg gagaatgga atgtaccgg tgtgaaaaa 
      tgcaaccgg gaatttccg aatttcaag taccgtctg 
      ttggtttcg atgaacgtg gccgattcg tcgagtagt 
      cagtgggtt actgttttt tcctcagaa gcggagaaa 
      attttgggg aaaacagcg caggaaatt ggggagctg 
      atggagacg gatcctgat actgtagca aatatgttt 
      gaagaggcg cagtttaag caatttatt ttcaagtgt 
      cgcgccaaa attgaaacc tacaatgat gagcagaaa 
      atgaagact gttgtggtg actgtggcg ccagtggat 
      tacgaggag tacaatgct tacctggtg gacaggatt 
      gaaaaattc actggagtt ccttgtaat tga
    mRNA sequence
    • >TC011844 RA
      cggcaagtc aaagtttgc gaattttgt cttttaatt 
      attgtaaaa attcagtga tattagtgc attttgtga 
      tggcttctg cgtacaaat tatcagaag gggcgcttc 
      agacgatta tgtacgggg gcgaggtca ttgaccctc 
      ttgttcaaa ttttgggga ctaaaaaaa tgtctccag 
      ggggtagcg acagcagct cgcgcttac gcctgatga 
      tttcggacg gtttgcaca aagtaaata ttgcagtgt 
      ttactgtgc caaagaacg accagactt tgccccacg 
      tcttagagc aggtgttta cgattatta aactaaagc 
      gctatcttt ccaacgtta taaaaaaca agaataaca 
      tagatgcgc gagttattg cgattttgg aactggaag 
      tggttgcgc gtggggagt ttgtaggtc agataatcg 
      gaaaccctg tctctttat ccgactctg aaaccttga 
      cgcaatcga gtgacgtta tcaagagaa atgagtgca 
      taaatggct cattttcct caaaaacaa tcagtccca 
      tagctagct taaacccct ttcacaaca actggataa 
      ttagggcga gagtcgtta ataagacga atgtgagaa 
      actggagca atgccaagg gggagggta agcttttca 
      gcatcgatt tggtcgaca aagggggcg aaatcagat 
      gcaccgctt tccgggacc ttgttgata agtttttcg 
      atcgccttg aggtcgaca aggtttact acattagca 
      aatgccagc tccagccaa tcaatcgcc aattttgct 
      ccttaaagc accagtttg aaatgtact tcaccccgg 
      aaacgatca tcgagccga gttatgacg acaatgaga 
      tcatcccac aaataacgt acaatttca ccacgattg 
      ataaagtag cggtcatgg aagctggga ccattgttg 
      acgtgatcg gcgtttgca agacaattt cggaactgc 
      agagttttg tggcgaaat caaccaatc gcgagatgc 
      agaaaagac agctcgtgt tagtcgatc agaccaaaa 
      ccgcgattt ctctgaatt tgtggggct cacaggcag 
      agtcgttcg cagtcaccg gtaatccgg tgattttgg 
      ttaaaaatg caaagattt atgaattta gaggtgcga 
      aaagtatca ctttgatgg gcactagtt tgcttaaaa 
      ttgacccag aaatacggg aaacccaca ttttgaggc 
      ggtggtacc attcggaag ggaatcagg tggaaataa 
      cgaatgtca gtcatagga acggatacg gatctacgc 
      catggctga cgttcaaag aagcgcgcc aacagacca 
      tttccgaaa aaccgcttt actaccaaa ctttcggga 
      cgattttgt tggttcgcc tcgacagag cggtgtaca 
      agtcgtgtc cgaacgctg attgccaga agaaggtcc 
      tcgatttgg agaatggaa tgtaccggt gtgaaaaat 
      gcaaccggg aatttccga atttcaagt accgtctgt 
      tggtttcga tgaacgtgg ccgattcgt cgagtagtc 
      agtgggtta ctgtttttt cctcagaag cggagaaaa 
      ttttgggga aaacagcgc aggaaattg gggagctga 
      tggagacgg atcctgata ctgtagcaa atatgtttg 
      aagaggcgc agtttaagc aatttattt tcaagtgtc 
      gcgccaaaa ttgaaacct acaatgatg agcagaaaa 
      tgaagactg ttgtggtga ctgtggcgc cagtggatt 
      acgaggagt acaatgctt acctggtgg acaggattg 
      aaaaattca ctggagttc cttgtaatt gattattgc 
      ttttgttgt gtaactttc gcaaataaa ttattgttt 
      tgttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY