Status
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Gene information
General information
TC011840 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12689906..12698004
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011840
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05111

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011840 RA
      MFHKADNPK GSFLEQTKA AREERAFEK RKERAATLI 
      QASVRGWLT RVKFSRDIL QEFDDTVPE VVEDGAKPS 
      LKPAVQIYN QSCRLLLIW RKERDKDRF EKLCRYLVA 
      TLECESPKF SYVGVALSK EHVIRWISH MNDILWKCC 
      EYLNDLKPE FAGDMKSIV LYLHVLVSF TSTSNWIVL 
      KHKNMEVLK SGMNQLCAN LMGQLFHKG FYLILKSLL 
      LRGLGRIKI AFKHATLSA ILTLALRPL IAANFSDKL 
      MTMFLIHIL SVPALIQHL DNLTPECIS TINNHKLFS 
      KSLELLSSQ QSMRIVFNT LEGSYALCL LANLLQLAY 
      MERDAALKD LSFPTFTVV VTGLLESCQ NYVVNKQSN 
      LTHWHPVLG WFAQPMDPA LNAAMPHVK IQLNLLWNS 
      PIVQILLGN LLQQLVLNV DSPQPSSSP QNMNFTGSN 
      FFKKVLESR GNKTNTQKN YRALGSPEV HRVVLVCSL 
      YHTALNTLT QLQLDILTG LCYQNSILY DLWLFLCSL 
      GPNCGLKTF LDHLAINTK CTAPEFQML QLFCDCMTH 
      YVTILDDVE MYEQQNPFK LTDFIVLSS FLNTFLYKA 
      VLGNLFDLK TIQTNTLFQ SLHTLLMLL YRRDCRRTY 
      TPQGHWLLK DVKISTFMA DLEKGRKAP QLLLQTMPH 
      IIPHEDRVR LFRKYITNE KTVLGLTES ACASPQSTL 
      ITVHRSRIV EDGYRQLAL LPPQALKGV IRVRFINEQ 
      GLDEAGIDQ DGVFKEFLE ESIKRIFDP SLNLFKVTS 
      EERLYPSPT SYLQDNHLQ LFEFVGRML GKAVYEGIV 
      VDVPFASFF LSQVSGQTA QALYSCVDE LPSLDPELY 
      RSLSYVKHY DGDVSDLDL TFSLDEECM GKLVTHELV 
      AGGKAVPVT NENKINYIH LMAHFRMHV QIKDQTSAF 
      IRGFRSIIN PDWLSLFST PELQRLISG DNVPLDMKD 
      LRKHTQYYG GFHDSHRVV GWLWDILEK DFTEEEKGM 
      FLKFVTSCS KPPLLGFAH LEPPFSIRC VEVGDDEDT 
      GDTIGSVFR GFFTIRKKD PQNRLPTSS TCFNLLKLP 
      NYQKKSTLR EKLRYAVTC NTGFELS
    CDS sequence
    • >TC011840 RA
      atgttccac aaggcggac aaccccaaa ggcagtttc 
      ctcgagcag accaaggct gcccgagag gagcgggcc 
      tttgagaag cggaaagaa agggcagcg actctgatc 
      caagccagt gtcaggggg tggctcacc cgagtcaag 
      ttctcccgc gatatttta caggagttt gatgacacg 
      gtgcccgag gttgtggaa gacggggcc aagccgagc 
      ctcaagccg gcggtgcaa atttacaac cagtcctgt 
      cggttgttg ctgatctgg cgcaaggag cgggacaaa 
      gacaggttt gagaagctg tgtcgatat ttagtcgct 
      acgctcgag tgcgaatcc cccaagttc agctacgtg 
      ggggtggca ctgagcaag gagcatgtg ataagatgg 
      atctctcat atgaatgat attttatgg aagtgctgt 
      gaatactta aatgacttg aaacccgag tttgctggt 
      gatatgaaa tcaattgtt ttgtatttg cacgtgtta 
      gtttcgttc actagcaca agtaactgg attgtgttg 
      aaacacaag aatatggaa gtcttgaag tctgggatg 
      aaccagttg tgcgccaat ttgatgggc cagttgttc 
      cacaaggga ttctatcta atactgaag agtctcttg 
      ttgaggggt ttagggaga attaaaatt gccttcaaa 
      catgctact ttatcggca attttgact ctggcctta 
      agaccgtta atagctgcc aacttttct gataagctg 
      atgacgatg tttcttatt cacatttta tcagttcct 
      gcgctgatt cagcatttg gataactta acacctgag 
      tgcatatct acgataaat aaccacaaa ttgttttca 
      aaaagcctc gaactgtta tcaagccag cagtcaatg 
      cgaatcgtc ttcaacacc ctcgaaggc agctacgcc 
      ctttgcctc cttgcaaac ctcctccag ttggcttac 
      atggaacga gacgctgcc ttaaaagac ttaagtttc 
      ccaactttc acagttgtt gttacgggt ttactcgaa 
      agttgccaa aattacgta gtcaataaa caatccaac 
      ctcacccac tggcaccca gtgctgggt tggttcgcc 
      cagcccatg gacccagca ttaaacgcg gccatgccg 
      cacgtaaaa atccaactc aatctctta tggaactca 
      ccgattgtt caaattcta ttgggcaat cttttacaa 
      caacttgtc ctaaatgtg gactcgccc cagccaagc 
      tcgagtcca caaaacatg aatttcact ggatcgaat 
      ttttttaag aaggttttg gaatctaga ggcaacaaa 
      accaacact cagaaaaat tatcgcgcg ctgggaagt 
      cccgaagtt catcgagtc gtgttagtg tgttcgctg 
      tatcatacg gctttgaat actttgact caactacag 
      ttggatatt ttaacaggt ttgtgttat cagaattca 
      attttgtac gatttgtgg ctgttcttg tgctcactt 
      ggaccaaat tgtgggctt aaaacgttc ctagatcat 
      cttgcaatt aacacaaag tgcacagct cccgagttt 
      caaatgttg caattattt tgtgattgc atgactcat 
      tatgtaacg attcttgat gatgtggaa atgtacgag 
      caacaaaac ccgtttaaa ttaaccgac tttattgtt 
      ttatcttcg tttttaaac acgtttctg tacaaagcc 
      gtcttaggg aatttattt gatttaaag acaattcag 
      accaacacg ctgttccag tccttacac actttatta 
      atgttgttg tatcggcga gactgtcgc agaacgtac 
      actcctcaa ggccactgg ttacttaaa gatgtaaaa 
      atatcgaca tttatggcc gatctagaa aagggccga 
      aaggcccct caattgctc ctccaaaca atgccacat 
      atcatacca cacgaggat agagtgcga ctatttcga 
      aaatacata acaaacgaa aaaaccgtt ttgggttta 
      accgagtcg gcttgtgcc tctccccag tcgactctg 
      atcacagtt cacaggtct cgaattgtt gaagacggc 
      tatcgccaa ctggccctc ctcccgccc caagccctt 
      aaaggcgta ataagggtt cgttttatc aacgagcaa 
      ggtctagac gaggccggg attgatcaa gacggcgtt 
      tttaaagag ttccttgaa gagagcatc aaacgtatt 
      tttgacccc tctctaaat ttgtttaaa gtaacaagt 
      gaggaacgt ttgtaccct tcgcccact tcatatttg 
      caagacaac cacttgcaa ttatttgaa tttgtgggc 
      cggatgctg ggcaaagcc gtctatgaa ggaattgta 
      gtcgatgtg cctttcgct tcgtttttt ctaagccag 
      gtttcagga caaacagct caagctttg tacagttgc 
      gtcgatgaa ttgccgtct ttggacccg gagttgtac 
      agaagttta agttatgtg aaacattac gatggagat 
      gtttccgat ttagactta acgtttagt ttagacgag 
      gaatgcatg gggaagttg gtcacgcat gagttggtg 
      gcggggggc aaagccgtc cccgtgaca aatgagaac 
      aagatcaat tacatccac ttgatggcc cacttccga 
      atgcacgtg caaatcaag gaccaaacc agcgctttc 
      attcgcggc ttccgctcg attatcaac cccgactgg 
      ttgtcgctc ttttcaaca cctgaattg caacgcctt 
      atctcgggt gataatgtc cccttagac atgaaagat 
      ttgcgcaaa cacactcag tattacggc ggttttcac 
      gactcgcac cgcgtggtg ggttggctg tgggatatt 
      ttagaaaag gatttcacc gaggaggag aaaggaatg 
      ttcctaaag tttgtgaca agttgttcc aaaccgccg 
      cttctaggc ttcgctcat ttggagccg cccttttcg 
      attaggtgt gtcgaagtt ggcgatgat gaagacacg 
      ggagacacg atcggaagc gtctttcgg gggtttttc 
      acgatacgg aaaaaggac ccacagaat cgcttaccg 
      acgtcttcc acttgtttc aatcttttg aagctgccc 
      aactaccaa aagaaaagc actttgagg gagaagctg 
      cggtacgct gtcacttgc aacactggg tttgagttg 
      tcgtaa
    mRNA sequence
    • >TC011840 RA
      tcagcgcga tgacagtga cagataaaa aacaatgac 
      cggtgttgc cagacccaa aataatatt gttcaaaag 
      tggaaaata tgtttggtg cttgttgtg tgcacagtg 
      attttgtgt gttgagatg ccaggtcag gggcaaaac 
      tgacgaatt tccacaatg ttccacaag gcggacaac 
      cccaaaggc agtttcctc gagcagacc aaggctgcc 
      cgagaggag cgggccttt gagaagcgg aaagaaagg 
      gcagcgact ctgatccaa gccagtgtc agggggtgg 
      ctcacccga gtcaagttc tcccgcgat attttacag 
      gagtttgat gacacggtg cccgaggtt gtggaagac 
      ggggccaag ccgagcctc aagccggcg gtgcaaatt 
      tacaaccag tcctgtcgg ttgttgctg atctggcgc 
      aaggagcgg gacaaagac aggtttgag aagctgtgt 
      cgatattta gtcgctacg ctcgagtgc gaatccccc 
      aagttcagc tacgtgggg gtggcactg agcaaggag 
      catgtgata agatggatc tctcatatg aatgatatt 
      ttatggaag tgctgtgaa tacttaaat gacttgaaa 
      cccgagttt gctggtgat atgaaatca attgttttg 
      tatttgcac gtgttagtt tcgttcact agcacaagt 
      aactggatt gtgttgaaa cacaagaat atggaagtc 
      ttgaagtct gggatgaac cagttgtgc gccaatttg 
      atgggccag ttgttccac aagggattc tatctaata 
      ctgaagagt ctcttgttg aggggttta gggagaatt 
      aaaattgcc ttcaaacat gctacttta tcggcaatt 
      ttgactctg gccttaaga ccgttaata gctgccaac 
      ttttctgat aagctgatg acgatgttt cttattcac 
      attttatca gttcctgcg ctgattcag catttggat 
      aacttaaca cctgagtgc atatctacg ataaataac 
      cacaaattg ttttcaaaa agcctcgaa ctgttatca 
      agccagcag tcaatgcga atcgtcttc aacaccctc 
      gaaggcagc tacgccctt tgcctcctt gcaaacctc 
      ctccagttg gcttacatg gaacgagac gctgcctta 
      aaagactta agtttccca actttcaca gttgttgtt 
      acgggttta ctcgaaagt tgccaaaat tacgtagtc 
      aataaacaa tccaacctc acccactgg cacccagtg 
      ctgggttgg ttcgcccag cccatggac ccagcatta 
      aacgcggcc atgccgcac gtaaaaatc caactcaat 
      ctcttatgg aactcaccg attgttcaa attctattg 
      ggcaatctt ttacaacaa cttgtccta aatgtggac 
      tcgccccag ccaagctcg agtccacaa aacatgaat 
      ttcactgga tcgaatttt tttaagaag gttttggaa 
      tctagaggc aacaaaacc aacactcag aaaaattat 
      cgcgcgctg ggaagtccc gaagttcat cgagtcgtg 
      ttagtgtgt tcgctgtat catacggct ttgaatact 
      ttgactcaa ctacagttg gatatttta acaggtttg 
      tgttatcag aattcaatt ttgtacgat ttgtggctg 
      ttcttgtgc tcacttgga ccaaattgt gggcttaaa 
      acgttccta gatcatctt gcaattaac acaaagtgc 
      acagctccc gagtttcaa atgttgcaa ttattttgt 
      gattgcatg actcattat gtaacgatt cttgatgat 
      gtggaaatg tacgagcaa caaaacccg tttaaatta 
      accgacttt attgtttta tcttcgttt ttaaacacg 
      tttctgtac aaagccgtc ttagggaat ttatttgat 
      ttaaagaca attcagacc aacacgctg ttccagtcc 
      ttacacact ttattaatg ttgttgtat cggcgagac 
      tgtcgcaga acgtacact cctcaaggc cactggtta 
      cttaaagat gtaaaaata tcgacattt atggccgat 
      ctagaaaag ggccgaaag gcccctcaa ttgctcctc 
      caaacaatg ccacatatc ataccacac gaggataga 
      gtgcgacta tttcgaaaa tacataaca aacgaaaaa 
      accgttttg ggtttaacc gagtcggct tgtgcctct 
      ccccagtcg actctgatc acagttcac aggtctcga 
      attgttgaa gacggctat cgccaactg gccctcctc 
      ccgccccaa gcccttaaa ggcgtaata agggttcgt 
      tttatcaac gagcaaggt ctagacgag gccgggatt 
      gatcaagac ggcgttttt aaagagttc cttgaagag 
      agcatcaaa cgtattttt gacccctct ctaaatttg 
      tttaaagta acaagtgag gaacgtttg tacccttcg 
      cccacttca tatttgcaa gacaaccac ttgcaatta 
      tttgaattt gtgggccgg atgctgggc aaagccgtc 
      tatgaagga attgtagtc gatgtgcct ttcgcttcg 
      ttttttcta agccaggtt tcaggacaa acagctcaa 
      gctttgtac agttgcgtc gatgaattg ccgtctttg 
      gacccggag ttgtacaga agtttaagt tatgtgaaa 
      cattacgat ggagatgtt tccgattta gacttaacg 
      tttagttta gacgaggaa tgcatgggg aagttggtc 
      acgcatgag ttggtggcg gggggcaaa gccgtcccc 
      gtgacaaat gagaacaag atcaattac atccacttg 
      atggcccac ttccgaatg cacgtgcaa atcaaggac 
      caaaccagc gctttcatt cgcggcttc cgctcgatt 
      atcaacccc gactggttg tcgctcttt tcaacacct 
      gaattgcaa cgccttatc tcgggtgat aatgtcccc 
      ttagacatg aaagatttg cgcaaacac actcagtat 
      tacggcggt tttcacgac tcgcaccgc gtggtgggt 
      tggctgtgg gatatttta gaaaaggat ttcaccgag 
      gaggagaaa ggaatgttc ctaaagttt gtgacaagt 
      tgttccaaa ccgccgctt ctaggcttc gctcatttg 
      gagccgccc ttttcgatt aggtgtgtc gaagttggc 
      gatgatgaa gacacggga gacacgatc ggaagcgtc 
      tttcggggg tttttcacg atacggaaa aaggaccca 
      cagaatcgc ttaccgacg tcttccact tgtttcaat 
      cttttgaag ctgcccaac taccaaaag aaaagcact 
      ttgagggag aagctgcgg tacgctgtc acttgcaac 
      actgggttt gagttgtcg taattgtta gtggaaatg 
      gaatttcga catatgtga cacccatat caacctttt 
      gtgttttaa tattacgat gatccgtgt tttttttct 
      ccaagccaa atattttat cagattaat ttattttaa 
      cccaatttg ttgactata atcacttcc atgttgtga 
      tgtctttgt tgtatttac ttatgtaat aaaatatat 
      tttaacga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY