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Gene information
General information
TC011836 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12813926..12819254
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011836
      iBeetle screen iB_05110

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011836 RA
        MCEVTKNNF KQKLPEIKR SLEKAKFIS IDLEFSALQ 
        PVEHQAPRL FDDNKERYA KLRLNLLNV IPVQVGITA 
        FHFDNKTND YFGEVFTFW VAPACFGDT DKAFYFQAS 
        TLSFLTMYK FDFNKFAYS GVPFLNSKD ARDLRLKLK 
        SGDLVGENC TLRHELDEA LKAHTPVIS KWYNSCKIG 
        DFLEIPDVY NRFGPFYEL LYFLHKHLR KRFKNLWTY 
        DRNGVFCVK KVTEADFTE LEKRCSLDD AILDDLTGF 
        KHVFDTLTA LKKPIVGHN VLQDLMIMI NNFENALPE 
        SYLQYKALA HQLFPTIFD TKTIYYELR ATIPREKLP 
        DDTSLSALF DYFRGGLGR HLAVDSTAI ECNIDQGKF 
        GSYHEAGWD SFCAGYIFL RMGYYNISQ HYPKSKTFV 
        FSEVVSGLA EYKNRVNVI RGAASHIKI DGEDPTSRR 
        PPWLIVESL KNEPLDVSD LMSTLSSYG FVDIKKLSS 
        RGDRVLVAV DNFRAAREI SKTFKTNPK FQVKQYNVL 
        RHSPLAQAV LLSAVTISG TFLLWLTHT MVKR
      CDS sequence
      • >TC011836 RA
        atgtgtgaa gtgactaaa aataatttc aaacaaaag 
        cttcctgaa ataaaaaga agtttagaa aaggcgaaa 
        tttatctca atcgatctg gaattcagt gcgttgcaa 
        cctgtggag catcaggcc ccaaggctt tttgacgac 
        aataaagag cggtacgcc aaactccgg ctgaacttg 
        ctcaacgtg atcccggtg caggtcggg atcactgcc 
        tttcacttc gacaataag actaacgat tatttcgga 
        gaggtgttc acgttttgg gtcgccccg gcctgcttt 
        ggggacacg gacaaggcg ttttacttc caagccagc 
        acgctcagc tttctaacc atgtacaag ttcgatttc 
        aataagttt gcttactca ggagttccg tttttaaac 
        agcaaggac gcacgcgac ttgagatta aagctaaaa 
        tcgggcgac ttagtcggg gaaaactgc acgttgcgc 
        cacgagctc gacgaggcc ttaaaagcc cacactccg 
        gtcatctcc aagtggtac aacagctgc aaaatcggc 
        gattttctg gaaatacca gacgtgtac aacaggttt 
        gggcctttc tacgaactg ctgtatttc ctccacaaa 
        catttgcgc aaacgcttc aagaatttg tggacgtac 
        gaccggaac ggcgttttt tgcgttaaa aaagtcaca 
        gaggctgat ttcaccgag ttggagaaa cgctgctcg 
        cttgacgac gcaatcctg gacgatttg acgggtttt 
        aagcacgtt tttgacact ctgactgcg ctcaagaag 
        ccgatagtt ggccacaac gtcctccag gaccttatg 
        attatgata aacaatttc gaaaacgcg ttgccagag 
        tcgtatttg cagtacaag gctttggcc caccagctc 
        ttccccacg attttcgac accaagacg atttattac 
        gagctgagg gcgacaatc ccaagggag aaattgccg 
        gatgatacg agcctttcg gcccttttt gactatttt 
        agaggggga ctggggcgc cacttggcc gtggacagc 
        acggcgatt gagtgcaac atcgaccag gggaaattt 
        gggagttac catgaagcg ggttgggat tcgttttgt 
        gcggggtat atttttctc aggatgggt tattataac 
        atttcgcag cattatccc aagtcgaaa actttcgtt 
        tttagcgaa gttgtgagc gggctggcg gagtacaaa 
        aatcgggtt aatgttatt agaggggca gcttcgcac 
        attaaaatc gatggagag gatccaact tcgcgaagg 
        cccccttgg ctgatcgtc gagtcgtta aaaaatgag 
        ccgctcgac gtgagtgat ctcatgtcc acgctgagc 
        tcgtacggc tttgtcgat atcaaaaaa ctgtcttcg 
        aggggcgac cgggtcctg gtcgccgtg gacaacttc 
        agggccgca cgggagatt tccaaaact ttcaaaacg 
        aaccccaaa tttcaagta aagcaatat aacgttctg 
        aggcactcg cccctggcc caggccgtg ctcttgagt 
        gctgtgacc atttcgggg acgtttctc ctgtggttg 
        actcacacg atggtgaag aggtaa
      mRNA sequence
      • >TC011836 RA
        cgccccgag ctcagctaa aacgaggaa gccaccacg 
        tgacgagtg tatttttaa agtttgagt tgaaaaatg 
        tgtgaagtg actaaaaat aatttcaaa caaaagctt 
        cctgaaata aaaagaagt ttagaaaag gcgaaattt 
        atctcaatc gatctggaa ttcagtgcg ttgcaacct 
        gtggagcat caggcccca aggcttttt gacgacaat 
        aaagagcgg tacgccaaa ctccggctg aacttgctc 
        aacgtgatc ccggtgcag gtcgggatc actgccttt 
        cacttcgac aataagact aacgattat ttcggagag 
        gtgttcacg ttttgggtc gccccggcc tgctttggg 
        gacacggac aaggcgttt tacttccaa gccagcacg 
        ctcagcttt ctaaccatg tacaagttc gatttcaat 
        aagtttgct tactcagga gttccgttt ttaaacagc 
        aaggacgca cgcgacttg agattaaag ctaaaatcg 
        ggcgactta gtcggggaa aactgcacg ttgcgccac 
        gagctcgac gaggcctta aaagcccac actccggtc 
        atctccaag tggtacaac agctgcaaa atcggcgat 
        tttctggaa ataccagac gtgtacaac aggtttggg 
        cctttctac gaactgctg tatttcctc cacaaacat 
        ttgcgcaaa cgcttcaag aatttgtgg acgtacgac 
        cggaacggc gttttttgc gttaaaaaa gtcacagag 
        gctgatttc accgagttg gagaaacgc tgctcgctt 
        gacgacgca atcctggac gatttgacg ggttttaag 
        cacgttttt gacactctg actgcgctc aagaagccg 
        atagttggc cacaacgtc ctccaggac cttatgatt 
        atgataaac aatttcgaa aacgcgttg ccagagtcg 
        tatttgcag tacaaggct ttggcccac cagctcttc 
        cccacgatt ttcgacacc aagacgatt tattacgag 
        ctgagggcg acaatccca agggagaaa ttgccggat 
        gatacgagc ctttcggcc ctttttgac tattttaga 
        gggggactg gggcgccac ttggccgtg gacagcacg 
        gcgattgag tgcaacatc gaccagggg aaatttggg 
        agttaccat gaagcgggt tgggattcg ttttgtgcg 
        gggtatatt tttctcagg atgggttat tataacatt 
        tcgcagcat tatcccaag tcgaaaact ttcgttttt 
        agcgaagtt gtgagcggg ctggcggag tacaaaaat 
        cgggttaat gttattaga ggggcagct tcgcacatt 
        aaaatcgat ggagaggat ccaacttcg cgaaggccc 
        ccttggctg atcgtcgag tcgttaaaa aatgagccg 
        ctcgacgtg agtgatctc atgtccacg ctgagctcg 
        tacggcttt gtcgatatc aaaaaactg tcttcgagg 
        ggcgaccgg gtcctggtc gccgtggac aacttcagg 
        gccgcacgg gagatttcc aaaactttc aaaacgaac 
        cccaaattt caagtaaag caatataac gttctgagg 
        cactcgccc ctggcccag gccgtgctc ttgagtgct 
        gtgaccatt tcggggacg tttctcctg tggttgact 
        cacacgatg gtgaagagg taacgacaa tttttaata 
        aaattttta cgcaaccac tgttttatt gtaaaaggc 
        tccctagta attttattc gtctcgggc caaggcttg 
        gacttaaat gagcgtaat gtggtgttt gcttgcata 
        atttcattt taaaaataa ccgagctac aatattcgc 
        gttattttc gctcgctat tgacttaat gacctatta 
        actcactta tttgaaaaa atgtcaagt cttgtaatt 
        aactgttta aaccgaaat cgcttaatt aatttcgct 
        tcgaggagt gtctattaa aagctcgta taaagtgat 
        gattgatcg gaagtttgt tttcaaggc aggtttcgc 
        aaacatgga gccaagttt atagacctc ggatttatc 
        cgacctcag gaattaata attgattgt tcttcacct 
        tgttatcga aatagtttc attttaaag tgccaaaat 
        aactcaagg ccaggattt tttgttcgc aagttccca 
        attaaaact tagatgctt gccttacgc ctgacgaca 
        taaaccgaa aacttgggt taatatcca ggaacttaa 
        gtttgatgt aaaaagttt gtacacatg gtgggtaaa 
        atcaaagct gcaattaag ttggaactt caactttca 
        ttgccacac tgaaagctc gcgcacttt ttgcttctt 
        ttgatgttt tgtggcaaa aaaaacctt ctagagtga 
        ttttttctt tattaagat ttattttta gtttgcttt 
        tgtgcgaaa taaaaatac acaaaattt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY