Status
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Gene information
General information
TC011833 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12927278..12978880
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011833
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05109

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011833 RA
      MSPSKTDSR RCVRPPHFD ILWLPRAAM RSVEGDPSD 
CVLVVGVSR PKMALAILT VMLISLFLT FHMIYDSAL 
SSLQTNARD HTRPPLLLL SRRLYPAAA RRLPQAIIM 
GVRKCGTRA LLEMLYLHP MVQKASGEV HFFDRDENY 
NKGLEWYRM QMPHSYHGQ ITIEKSPSY FVTPEVPER 
IRAMNASVK LLLIVREPV TRAISDYTQ LRANAATAS 
PTTSSPPPR SFESLSVFP NGSINEAYR PLAISLYHN 
YLHRWLEVF PREQILVVN GDLLIEDPV PQVQKIERF 
LGLEPRIGT HNFYFNETK GFYCLRNET SDRCLRETK 
GRKHPRVDP NVVGKLRRY FGEHNQKFY ELIGEDLGW 
PEE
    CDS sequence
    • >TC011833 RA
      atgagcccg tctaaaacc gattccaga agatgtgtg 
cggccgccg cacttcgat atcctatgg ttgccccgt 
gcagcaatg aggtccgtg gagggcgat ccgtcagac 
tgcgtcctc gtcgtggga gtctcccgg ccgaagatg 
gccctggcc atcctcacc gtcatgcta atctccctc 
ttcttgact ttccacatg atctacgac agcgccctt 
agcagcctc cagacgaac gcccgagac cacacccgg 
ccgccgctg ctgctgctc tccaggagg ctgtacccg 
gcggcggcg cgccgcctg ccccaggcc atcatcatg 
ggggtgcgg aagtgcggg accagggcg ctgctcgag 
atgctctat ttgcatccg atggtgcag aaggcttcc 
ggagaggtg catttcttc gacagggat gagaactac 
aacaaaggc ctggagtgg tatcggatg cagatgccg 
cattcctat cacggccag atcacgatc gagaagagt 
cccagttat tttgttacg ccggaagtc ccggagcgg 
atccgcgcg atgaacgcc agcgtgaag ctgctgcta 
atagtccgg gagccggtg accagagcc atatcggac 
tacacccag ctgcgggcc aacgccgcg accgcctcc 
cccaccacc tccagcccg ccccccagg tccttcgag 
agcctgtcg gtgttcccc aacggctcc atcaacgag 
gcgtaccgg ccgctggcc atctccctc taccacaac 
tacctgcac cggtggctg gaggtgttc ccgcgggag 
cagatcctg gtggtgaac ggcgacctc ctcatcgag 
gaccccgtg ccccaggtc cagaagatc gagcgcttc 
ctgggcctg gagccgcgc atcggcacg cacaacttc 
tacttcaac gagaccaag ggcttctac tgcctgcgg 
aacgagacc tcggaccgg tgtctgagg gagaccaag 
ggccggaag caccccagg gtcgacccc aacgtcgtc 
ggcaagctg cgcaggtac ttcggggag cacaatcaa 
aagttctac gagcttatt ggcgaggac ctcggctgg 
cctgaagaa taa
    mRNA sequence
    • >TC011833 RA
      gggatgcgt ggtggcggc acaatatcg atcgcgacg 
gcggcggcg cgttcgcgc cgtcgagac gcagcagca 
catagtaga aatcggtgt tcctgacag gttcgaaca 
gagtgcggc gcgaccacc atggaacta caacacgga 
cggtagtga agtgctgtt tttccttgc tgtagtttc 
agacgaggt ggaacaaca gtgctgtag ggaggcagc 
gacagctcg agggataag ctgacaaaa aacgtgaaa 
aatgaagaa taaaatcga gcacctgcg acaatgagc 
ccgtctaaa accgattcc agaagatgt gtgcggccg 
ccgcacttc gatatccta tggttgccc cgtgcagca 
atgaggtcc gtggagggc gatccgtca gactgcgtc 
ctcgtcgtg ggagtctcc cggccgaag atggccctg 
gccatcctc accgtcatg ctaatctcc ctcttcttg 
actttccac atgatctac gacagcgcc cttagcagc 
ctccagacg aacgcccga gaccacacc cggccgccg 
ctgctgctg ctctccagg aggctgtac ccggcggcg 
gcgcgccgc ctgccccag gccatcatc atgggggtg 
cggaagtgc gggaccagg gcgctgctc gagatgctc 
tatttgcat ccgatggtg cagaaggct tccggagag 
gtgcatttc ttcgacagg gatgagaac tacaacaaa 
ggcctggag tggtatcgg atgcagatg ccgcattcc 
tatcacggc cagatcacg atcgagaag agtcccagt 
tattttgtt acgccggaa gtcccggag cggatccgc 
gcgatgaac gccagcgtg aagctgctg ctaatagtc 
cgggagccg gtgaccaga gccatatcg gactacacc 
cagctgcgg gccaacgcc gcgaccgcc tcccccacc 
acctccagc ccgcccccc aggtccttc gagagcctg 
tcggtgttc cccaacggc tccatcaac gaggcgtac 
cggccgctg gccatctcc ctctaccac aactacctg 
caccggtgg ctggaggtg ttcccgcgg gagcagatc 
ctggtggtg aacggcgac ctcctcatc gaggacccc 
gtgccccag gtccagaag atcgagcgc ttcctgggc 
ctggagccg cgcatcggc acgcacaac ttctacttc 
aacgagacc aagggcttc tactgcctg cggaacgag 
acctcggac cggtgtctg agggagacc aagggccgg 
aagcacccc agggtcgac cccaacgtc gtcggcaag 
ctgcgcagg tacttcggg gagcacaat caaaagttc 
tacgagctt attggcgag gacctcggc tggcctgaa 
gaataattg tttagttgt tagtttaag ccgggtcgg 
ggtcactgt atatgctag gtgcggtag agtaggtcc 
accggggtg gggggatgc aatgactac accgtgtag 
aggcgagga attttcttc ttaataagc acaattgat 
gaaagcgga gtttgcgag gtaaaaagg cggccttaa 
tgcgtagtg aattataaa tcagacggc gcacgaggc 
ggagctttt ctccgtggg aaagtttgt taggaaggg 
gatgatagt gtttaaaag taggcgact tcctgctgg 
ttacttatt gcaaacatt ctcgctcca tactcgaag 
taacaatag ccgaagaga gcacaaagt gtgcacaat 
tattgctca attcatagt cgaagacaa aggaaacgg 
atacatatc acaaagtac aggcacttg aaatgaaaa 
tgacgccgc cgtaaagac cagagaccg gaaactagt 
ttggaattt ttttgttga caaacaata caattttca 
agtttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY