Status
Please wait ...
Gene information
General information
TC011827 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13069570..13070467
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011827
    iBeetle screen iB_05108

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011827 RA
      MLHSRVFLF ILLSVSVKC ELCSWKENF EQVAKCAAV 
      RALKSFEIA ERQDGLEIM PGVALQRNG SYSGRSAKK 
      LEFSVRTAN TSELLDLLL SQASRFLNS RVLQIKLPL 
      QVPQNLARS FEEARGKVK KTMGGLILG LGTRMMSMI 
      PVMFGGLVL LTTKALIVG KLAFVISII LFIQVFFSG 
      RPFPFFNYG GGTFNIPPT YGVPTTYGT NFGTNFGTN 
      SLGGWSSAQ NPYARSFNV SQNESNSED YS
    CDS sequence
    • >TC011827 RA
      atgttgcac tctcgagtc ttccttttc attttactt 
      tcagtgagt gtgaagtgc gagttatgt tcgtggaag 
      gagaatttc gagcaagtg gcgaaatgt gcagctgtg 
      cgcgctttg aagtcgttt gaaattgcc gaaagacag 
      gacgggttg gaaataatg ccaggggtt gcgctccaa 
      agaaatggg tcctattct gggagaagt gcaaagaag 
      ctggagttt tcggtccgg acggctaac acgagcgaa 
      ctcctggac ttgctttta agccaagcg tcgcgtttc 
      ctcaattct agggttttg caaattaag ttgccactg 
      caagtgccg caaaaccta gccaggtct ttcgaggaa 
      gcccgtggc aaggtgaag aagacaatg ggtggccta 
      attctgggg cttgggacc aggatgatg tccatgatt 
      ccggtcatg ttcgggggc ctcgtgctt ttgacgacg 
      aaggccctc attgtgggg aaattagcc ttcgttatc 
      tctattatc ctattcatt caggtgttc ttcagcgga 
      cggcctttt ccgttcttt aattatgga ggaggtact 
      tttaacatt ccgccgact tatggggta cctaccact 
      tatggtacc aattttggg accaacttt ggtaccaac 
      agcctgggc ggatggagt tccgcgcaa aatccatac 
      gctaggtcg ttcaatgtt tcgcaaaat gagagcaac 
      agtgaggac tattcgtaa
    mRNA sequence
    • >TC011827 RA
      attttcacg cctccaccg aaaatgttg cactctcga 
      gtcttcctt ttcatttta ctttcagtg agtgtgaag 
      tgcgagtta tgttcgtgg aaggagaat ttcgagcaa 
      gtggcgaaa tgtgcagct gtgcgcgct ttgaagtcg 
      tttgaaatt gccgaaaga caggacggg ttggaaata 
      atgccaggg gttgcgctc caaagaaat gggtcctat 
      tctgggaga agtgcaaag aagctggag ttttcggtc 
      cggacggct aacacgagc gaactcctg gacttgctt 
      ttaagccaa gcgtcgcgt ttcctcaat tctagggtt 
      ttgcaaatt aagttgcca ctgcaagtg ccgcaaaac 
      ctagccagg tctttcgag gaagcccgt ggcaaggtg 
      aagaagaca atgggtggc ctaattctg gggcttggg 
      accaggatg atgtccatg attccggtc atgttcggg 
      ggcctcgtg cttttgacg acgaaggcc ctcattgtg 
      gggaaatta gccttcgtt atctctatt atcctattc 
      attcaggtg ttcttcagc ggacggcct tttccgttc 
      tttaattat ggaggaggt acttttaac attccgccg 
      acttatggg gtacctacc acttatggt accaatttt 
      gggaccaac tttggtacc aacagcctg ggcggatgg 
      agttccgcg caaaatcca tacgctagg tcgttcaat 
      gtttcgcaa aatgagagc aacagtgag gactattcg 
      taacaattg gaataaaaa aatattata agc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY