Status
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Gene information
General information
TC034435
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011802,TC011803 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13363234..13386013
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034435
    iBeetle screen iB_05106

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034435 RA
      MNSPLPQSP RTRRQSGGR RPPTHLHSD CPPEPEDCD 
APGLGQRSV SLTTLQPTI IQPRMAQLE TLEAKMASI 
EVSLSSTPR RKKNTGINA SIRSAPKEI AAKELETLR 
NALRDKENI IQSLKGQLT IPGLRLTQM RNPSNTNRE 
LTEVEKKQA EERLNRLKT DVDNKRLAI KNLKMALER 
LDITDNIDV RIQQAELEY QLGREELNL LTLLEETRA 
LQLCLDESN KNSSESHTL YSCVNGSVH VILRGVEVE 
FDPKSPKFG ASQKEAVPG LWIDWALEE TGLCKGDRL 
VEVNGKIVL TKNRDDLTR LLAAAPDPA QIVILRKLP 
QNGSTPTIA TTSKEVASL RCELEAVRE RAEEAQKTK 
ENLISDNIR LTHRISYLE EQVSELLCR KKSDTDVRI 
LSKTPVIST VKTNQNVTN INITTPPPK DLQVFQKGQ 
VTALVANIP GLETSKDTL PVRSKSSLS NVSNTHIPA 
PDHHCHKPH RHKHRSRLQ TSTSTQHLD QNDYRKHNH 
HHHHREKDY SSETNSAIE HVSRYNKRN TENGFEHIM 
DQSYKKATK IVHELTRDT LYEKHRQKC LTASEKYNS 
DILKHYNAR KSASVLDFR SEIHIGPKC PDSKSVEDL 
EERKAKRIQ DARSVKSLD FDSDCTSLK SKSIDYTSE 
PTESRKLSG YGGCCEVKA RPTPPKKPL RLSLHKTQS 
LQSVETPTP GSSPGKGES RKRNYKGEM GLQNGCQEV 
KWAGFGHKL ENGVESGSW C
    CDS sequence
    • >TC034435 RA
      atgaattcg ccgctcccg cagagtccc cggacccgc 
aggcaatcc gggggtcgt cgtcccccg acccacctc 
cactcggac tgtcctccg gagcccgag gactgcgac 
gcgcccgga ctgggacaa agatcggtc tctttgacg 
actcttcag ccgaccatc atccagccc agaatggcc 
caactggag acactggaa gccaagatg gccagcatc 
gaggtgtcc ctctcgagc acacccaga cgcaaaaag 
aacaccgga attaacgcc tcgatccgg tccgctccc 
aaggagatc gccgccaag gagctggag acgttgagg 
aacgcgctg cgggacaag gaaaatatc atacagagc 
ttgaagggc cagctgacc atccccgga ctgaggctg 
acgcagatg cgcaacccc tccaacacc aatcgggag 
ctgacagaa gttgagaaa aaacaagcc gaagaaagg 
ctcaatagg ctcaagacg gacgtggat aacaagcgc 
ttggctatc aagaacctc aaaatggct ctggaacgg 
ctggatatc actgacaac atcgacgtg cggatccag 
caggccgag ctcgagtac cagctgggt cgcgaagag 
ctcaatttg ttaacactg ctcgaggaa acgcgcgct 
ctccaatta tgtctcgat gagtcgaac aaaaactcg 
tcggaatct catactttg tacagttgc gttaacgga 
agcgtccac gtgatcctt cgaggtgtc gaggtcgag 
ttcgacccg aaaagcccc aagttcggg gcgagtcag 
aaggaggcc gtgccgggc ttgtggata gattgggcg 
ctcgaggaa acggggctg tgcaaagga gacagattg 
gtcgaagtc aacggcaaa atcgtcttg accaaaaac 
cgagacgac ctcactcgc ctccttgcc gcagcaccg 
gacccagcc cagatagta attttgcga aaactgccc 
caaaacggc tccaccccc actattgcc accacctcc 
aaagaggtg gcctcgtta cgctgcgag ctggaggcg 
gtgcgcgaa cgcgccgaa gaggcgcaa aaaacgaaa 
gaaaacctc atctcggac aacatcaga ctgacacac 
cgaatttcc tatctggag gaacaagtt tccgagctc 
ctctgtcgc aagaaatcc gacaccgac gtccggatc 
ttatcgaaa actccggtg atttcgacg gtgaaaacc 
aaccaaaac gtcacaaac atcaacata acgacgcca 
ccaccgaag gacctccag gtgttccaa aagggccag 
gttacggcc ctcgtggcc aacatcccc gggctggag 
acgtccaaa gacacgctc ccagtgcgc tccaaaagc 
agtctctcg aacgtttcc aacacccat atcccggcg 
ccggaccac cattgccac aagccccat cggcacaag 
caccgctca cgcctccag acctccacc agcacccag 
cacctggac cagaacgac taccgcaaa cacaaccac 
caccaccac caccgggag aaagactac agctcggag 
accaactcg gccattgag cacgtctcc aggtacaac 
aaacgcaac actgagaac ggcttcgag cacatcatg 
gaccaaagc tacaagaaa gcaacgaaa atcgtccac 
gagctcacg cgagacacc ctctacgag aagcacaga 
cagaagtgc ctcacagcc tccgagaag tacaactcg 
gacatcctc aagcactac aacgcccgg aagtcggcg 
tcggtgctg gacttccgc tcggaaatc cacataggg 
cccaagtgc ccagactcg aagagtgtt gaagacctg 
gaggagaga aaagccaag aggatccaa gacgcgcgc 
agcgtcaag tcgctggat tttgacagc gattgcacg 
agtctcaag tccaagtcg attgattac acttcggag 
ccgactgag agccggaaa ttgtcggga tatgggggg 
tgttgcgag gtgaaggcg cggccgacg ccgccgaag 
aagccgctg cggctgtcg ctgcacaaa acgcagagt 
ttgcagtcg gtggagacg cccactccg gggagttcc 
cccgggaag ggcgagtcc cggaaacgg aactacaag 
ggggagatg gggctgcag aacggctgc caggaggtc 
aagtgggcc gggtttggc cacaagctg gagaacgga 
gtggagagc gggagctgg tgttag
    mRNA sequence
    • >TC034435 RA
      cttgaaccg aaccaactc tagtgctcc agtttcagt 
gttgtgacc gcttgaatg gattatttt taaaacagg 
gcgcgatgc tgggatgaa ttcgccgct cccgcagag 
tccccggac ccgcaggca atccggggg tcgtcgtcc 
cccgaccca cctccactc ggactgtcc tccggagcc 
cgaggactg cgacgcgcc cggactggg acaaagatc 
ggtctcttt gacgactct tcagccgac catcatcca 
gcccagaat ggcccaact ggagacact ggaagccaa 
gatggccag catcgaggt gtccctctc gagcacacc 
cagacgcaa aaagaacac cggaattaa cgcctcgat 
ccggtccgc tcccaagga gatcgccgc caaggagct 
ggagacgtt gaggaacgc gctgcggga caaggaaaa 
tatcataca gagcttgaa gggccagct gaccatccc 
cggactgag gctgacgca gatgcgcaa cccctccaa 
caccaatcg ggagctgac agaagttga gaaaaaaca 
agccgaaga aaggctcaa taggctcaa gacggacgt 
ggataacaa gcgcttggc tatcaagaa cctcaaaat 
ggctctgga acggctgga tatcactga caacatcga 
cgtgcggat ccagcaggc cgagctcga gtaccagct 
gggtcgcga agagctcaa tttgttaac actgctcga 
ggaaacgcg cgctctcca attatgtct cgatgagtc 
gaacaaaaa ctcgtcgga atctcatac tttgtacag 
ttgcgttaa cggaagcgt ccacgtgat ccttcgagg 
tgtcgaggt cgagttcga cccgaaaag ccccaagtt 
cggggcgag tcagaagga ggccgtgcc gggcttgtg 
gatagattg ggcgctcga ggaaacggg gctgtgcaa 
aggagacag attggtcga agtcaacgg caaaatcgt 
cttgaccaa aaaccgaga cgacctcac tcgcctcct 
tgccgcagc accggaccc agcccagat agtaatttt 
gcgaaaact gccccaaaa cggctccac ccccactat 
tgccaccac ctccaaaga ggtggcctc gttacgctg 
cgagctgga ggcggtgcg cgaacgcgc cgaagaggc 
gcaaaaaac gaaagaaaa cctcatctc ggacaacat 
cagactgac acaccgaat ttcctatct ggaggaaca 
agtttccga gctcctctg tcgcaagaa atccgacac 
cgacgtccg gatcttatc gaaaactcc ggtgatttc 
gacggtgaa aaccaacca aaacgtcac aaacatcaa 
cataacgac gccaccacc gaaggacct ccaggtgtt 
ccaaaaggg ccaggttac ggccctcgt ggccaacat 
ccccgggct ggagacgtc caaagacac gctcccagt 
gcgctccaa aagcagtct ctcgaacgt ttccaacac 
ccatatccc ggcgccgga ccaccattg ccacaagcc 
ccatcggca caagcaccg ctcacgcct ccagacctc 
caccagcac ccagcacct ggaccagaa cgactaccg 
caaacacaa ccaccacca ccaccaccg ggagaaaga 
ctacagctc ggagaccaa ctcggccat tgagcacgt 
ctccaggta caacaaacg caacactga gaacggctt 
cgagcacat catggacca aagctacaa gaaagcaac 
gaaaatcgt ccacgagct cacgcgaga caccctcta 
cgagaagca cagacagaa gtgcctcac agcctccga 
gaagtacaa ctcggacat cctcaagca ctacaacgc 
ccggaagtc ggcgtcggt gctggactt ccgctcgga 
aatccacat agggcccaa gtgcccaga ctcgaagag 
tgttgaaga cctggagga gagaaaagc caagaggat 
ccaagacgc gcgcagcgt caagtcgct ggattttga 
cagcgattg cacgagtct caagtccaa gtcgattga 
ttacacttc ggagccgac tgagagccg gaaattgtc 
gggatatgg ggggtgttg cgaggtgaa ggcgcggcc 
gacgccgcc gaagaagcc gctgcggct gtcgctgca 
caaaacgca gagtttgca gtcggtgga gacgcccac 
tccggggag ttcccccgg gaagggcga gtcccggaa 
acggaacta caaggggga gatggggct gcagaacgg 
ctgccagga ggtcaagtg ggccgggtt tggccacaa 
gctggagaa cggagtgga gagcgggag ctggtgtta 
gtgtaaatt tgtagactg atacgattt tataataaa 
tatttaaat accta
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