| General information | |||
| TC034435
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 13363234..13386013 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034435 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05106 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC034435 RA
MNSPLPQSP RTRRQSGGR RPPTHLHSD CPPEPEDCD APGLGQRSV SLTTLQPTI IQPRMAQLE TLEAKMASI EVSLSSTPR RKKNTGINA SIRSAPKEI AAKELETLR NALRDKENI IQSLKGQLT IPGLRLTQM RNPSNTNRE LTEVEKKQA EERLNRLKT DVDNKRLAI KNLKMALER LDITDNIDV RIQQAELEY QLGREELNL LTLLEETRA LQLCLDESN KNSSESHTL YSCVNGSVH VILRGVEVE FDPKSPKFG ASQKEAVPG LWIDWALEE TGLCKGDRL VEVNGKIVL TKNRDDLTR LLAAAPDPA QIVILRKLP QNGSTPTIA TTSKEVASL RCELEAVRE RAEEAQKTK ENLISDNIR LTHRISYLE EQVSELLCR KKSDTDVRI LSKTPVIST VKTNQNVTN INITTPPPK DLQVFQKGQ VTALVANIP GLETSKDTL PVRSKSSLS NVSNTHIPA PDHHCHKPH RHKHRSRLQ TSTSTQHLD QNDYRKHNH HHHHREKDY SSETNSAIE HVSRYNKRN TENGFEHIM DQSYKKATK IVHELTRDT LYEKHRQKC LTASEKYNS DILKHYNAR KSASVLDFR SEIHIGPKC PDSKSVEDL EERKAKRIQ DARSVKSLD FDSDCTSLK SKSIDYTSE PTESRKLSG YGGCCEVKA RPTPPKKPL RLSLHKTQS LQSVETPTP GSSPGKGES RKRNYKGEM GLQNGCQEV KWAGFGHKL ENGVESGSW C
- >TC034435 RA
atgaattcg ccgctcccg cagagtccc cggacccgc aggcaatcc gggggtcgt cgtcccccg acccacctc cactcggac tgtcctccg gagcccgag gactgcgac gcgcccgga ctgggacaa agatcggtc tctttgacg actcttcag ccgaccatc atccagccc agaatggcc caactggag acactggaa gccaagatg gccagcatc gaggtgtcc ctctcgagc acacccaga cgcaaaaag aacaccgga attaacgcc tcgatccgg tccgctccc aaggagatc gccgccaag gagctggag acgttgagg aacgcgctg cgggacaag gaaaatatc atacagagc ttgaagggc cagctgacc atccccgga ctgaggctg acgcagatg cgcaacccc tccaacacc aatcgggag ctgacagaa gttgagaaa aaacaagcc gaagaaagg ctcaatagg ctcaagacg gacgtggat aacaagcgc ttggctatc aagaacctc aaaatggct ctggaacgg ctggatatc actgacaac atcgacgtg cggatccag caggccgag ctcgagtac cagctgggt cgcgaagag ctcaatttg ttaacactg ctcgaggaa acgcgcgct ctccaatta tgtctcgat gagtcgaac aaaaactcg tcggaatct catactttg tacagttgc gttaacgga agcgtccac gtgatcctt cgaggtgtc gaggtcgag ttcgacccg aaaagcccc aagttcggg gcgagtcag aaggaggcc gtgccgggc ttgtggata gattgggcg ctcgaggaa acggggctg tgcaaagga gacagattg gtcgaagtc aacggcaaa atcgtcttg accaaaaac cgagacgac ctcactcgc ctccttgcc gcagcaccg gacccagcc cagatagta attttgcga aaactgccc caaaacggc tccaccccc actattgcc accacctcc aaagaggtg gcctcgtta cgctgcgag ctggaggcg gtgcgcgaa cgcgccgaa gaggcgcaa aaaacgaaa gaaaacctc atctcggac aacatcaga ctgacacac cgaatttcc tatctggag gaacaagtt tccgagctc ctctgtcgc aagaaatcc gacaccgac gtccggatc ttatcgaaa actccggtg atttcgacg gtgaaaacc aaccaaaac gtcacaaac atcaacata acgacgcca ccaccgaag gacctccag gtgttccaa aagggccag gttacggcc ctcgtggcc aacatcccc gggctggag acgtccaaa gacacgctc ccagtgcgc tccaaaagc agtctctcg aacgtttcc aacacccat atcccggcg ccggaccac cattgccac aagccccat cggcacaag caccgctca cgcctccag acctccacc agcacccag cacctggac cagaacgac taccgcaaa cacaaccac caccaccac caccgggag aaagactac agctcggag accaactcg gccattgag cacgtctcc aggtacaac aaacgcaac actgagaac ggcttcgag cacatcatg gaccaaagc tacaagaaa gcaacgaaa atcgtccac gagctcacg cgagacacc ctctacgag aagcacaga cagaagtgc ctcacagcc tccgagaag tacaactcg gacatcctc aagcactac aacgcccgg aagtcggcg tcggtgctg gacttccgc tcggaaatc cacataggg cccaagtgc ccagactcg aagagtgtt gaagacctg gaggagaga aaagccaag aggatccaa gacgcgcgc agcgtcaag tcgctggat tttgacagc gattgcacg agtctcaag tccaagtcg attgattac acttcggag ccgactgag agccggaaa ttgtcggga tatgggggg tgttgcgag gtgaaggcg cggccgacg ccgccgaag aagccgctg cggctgtcg ctgcacaaa acgcagagt ttgcagtcg gtggagacg cccactccg gggagttcc cccgggaag ggcgagtcc cggaaacgg aactacaag ggggagatg gggctgcag aacggctgc caggaggtc aagtgggcc gggtttggc cacaagctg gagaacgga gtggagagc gggagctgg tgttag
- >TC034435 RA
cttgaaccg aaccaactc tagtgctcc agtttcagt gttgtgacc gcttgaatg gattatttt taaaacagg gcgcgatgc tgggatgaa ttcgccgct cccgcagag tccccggac ccgcaggca atccggggg tcgtcgtcc cccgaccca cctccactc ggactgtcc tccggagcc cgaggactg cgacgcgcc cggactggg acaaagatc ggtctcttt gacgactct tcagccgac catcatcca gcccagaat ggcccaact ggagacact ggaagccaa gatggccag catcgaggt gtccctctc gagcacacc cagacgcaa aaagaacac cggaattaa cgcctcgat ccggtccgc tcccaagga gatcgccgc caaggagct ggagacgtt gaggaacgc gctgcggga caaggaaaa tatcataca gagcttgaa gggccagct gaccatccc cggactgag gctgacgca gatgcgcaa cccctccaa caccaatcg ggagctgac agaagttga gaaaaaaca agccgaaga aaggctcaa taggctcaa gacggacgt ggataacaa gcgcttggc tatcaagaa cctcaaaat ggctctgga acggctgga tatcactga caacatcga cgtgcggat ccagcaggc cgagctcga gtaccagct gggtcgcga agagctcaa tttgttaac actgctcga ggaaacgcg cgctctcca attatgtct cgatgagtc gaacaaaaa ctcgtcgga atctcatac tttgtacag ttgcgttaa cggaagcgt ccacgtgat ccttcgagg tgtcgaggt cgagttcga cccgaaaag ccccaagtt cggggcgag tcagaagga ggccgtgcc gggcttgtg gatagattg ggcgctcga ggaaacggg gctgtgcaa aggagacag attggtcga agtcaacgg caaaatcgt cttgaccaa aaaccgaga cgacctcac tcgcctcct tgccgcagc accggaccc agcccagat agtaatttt gcgaaaact gccccaaaa cggctccac ccccactat tgccaccac ctccaaaga ggtggcctc gttacgctg cgagctgga ggcggtgcg cgaacgcgc cgaagaggc gcaaaaaac gaaagaaaa cctcatctc ggacaacat cagactgac acaccgaat ttcctatct ggaggaaca agtttccga gctcctctg tcgcaagaa atccgacac cgacgtccg gatcttatc gaaaactcc ggtgatttc gacggtgaa aaccaacca aaacgtcac aaacatcaa cataacgac gccaccacc gaaggacct ccaggtgtt ccaaaaggg ccaggttac ggccctcgt ggccaacat ccccgggct ggagacgtc caaagacac gctcccagt gcgctccaa aagcagtct ctcgaacgt ttccaacac ccatatccc ggcgccgga ccaccattg ccacaagcc ccatcggca caagcaccg ctcacgcct ccagacctc caccagcac ccagcacct ggaccagaa cgactaccg caaacacaa ccaccacca ccaccaccg ggagaaaga ctacagctc ggagaccaa ctcggccat tgagcacgt ctccaggta caacaaacg caacactga gaacggctt cgagcacat catggacca aagctacaa gaaagcaac gaaaatcgt ccacgagct cacgcgaga caccctcta cgagaagca cagacagaa gtgcctcac agcctccga gaagtacaa ctcggacat cctcaagca ctacaacgc ccggaagtc ggcgtcggt gctggactt ccgctcgga aatccacat agggcccaa gtgcccaga ctcgaagag tgttgaaga cctggagga gagaaaagc caagaggat ccaagacgc gcgcagcgt caagtcgct ggattttga cagcgattg cacgagtct caagtccaa gtcgattga ttacacttc ggagccgac tgagagccg gaaattgtc gggatatgg ggggtgttg cgaggtgaa ggcgcggcc gacgccgcc gaagaagcc gctgcggct gtcgctgca caaaacgca gagtttgca gtcggtgga gacgcccac tccggggag ttcccccgg gaagggcga gtcccggaa acggaacta caaggggga gatggggct gcagaacgg ctgccagga ggtcaagtg ggccgggtt tggccacaa gctggagaa cggagtgga gagcgggag ctggtgtta gtgtaaatt tgtagactg atacgattt tataataaa tatttaaat accta
