Status
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Gene information
General information
TC011791 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13469948..13472564
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011791
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05105

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011791 RA
      MSYPGYPPY QAGSNYPPN PNSGSPYPP NPPYPQQGG 
      VYPPSVGYT SGSPMYMPQ PYGGGGYPP QTGGSPYPQ 
      PSPYPSQGA GPYPGQGSS PYPNQGASP YPPPGGQGA 
      APYPSQGGH QGPSPYPSQ PYGQGHQTP SYTPSYSQG 
      QHTIKRSPT VVPAHPFNP REDAEILRK AMKGFGTDE 
      KAIINVLAR RTNAQRLEI EVQFKTLYG KDLISDLKS 
      ELTGNFENL IVAMMTPLP QYYAREIHD AISGVGTDE 
      DVLIETMCT LSNAEIRTI RDAYHRTYY QNLESDLKG 
      DTSGHFRRL MVSLCSAGR DESMQTNPQ AATADAQAL 
      LRAGELQVG TDESTFNMI LCQRNHAQL RLIFEEYQR 
      LTGHDIEKA IKNEFSGDI EEGLLAVVR SIKNQPAFF 
      AKRLNKSMK GMGTNDRDL IRLVVTRSE IDMGDIKRE 
      YQAKYGESL ADAIKGDCS GDYKKCLLA LIGES
    CDS sequence
    • >TC011791 RA
      atgagttat ccaggatat ccaccctat caagcgggc 
      tcaaactac ccgccgaac ccaaattcc gggtcacct 
      tatcccccc aatccccct tatccccaa caagggggc 
      gtttatccc cccagtgtt gggtatact tcaggctcc 
      cccatgtac atgccgcaa ccctacggc ggtggcggg 
      tatcctcct caaaccggg ggttccccc tatccacaa 
      cctagtccc tatcccagt caaggggca ggtccctac 
      ccaggtcag ggatcctct ccctacccc aaccagggg 
      gctagtccc tatcctcct ccaggtggc caaggagcc 
      gctccttac cccagtcaa gggggccat caaggaccc 
      tctccttac ccctcccaa ccctatggc caaggacac 
      cagacccct agctataca ccctcttat agtcaaggc 
      caacacaca atcaagaga tccccgact gtggttccc 
      gcgcaccct ttcaaccct cgcgaagat gccgaaatc 
      ttgcgcaag gctatgaaa ggtttcgga acggatgaa 
      aaagccatc atcaatgtc ctagcccgc cgtaccaac 
      gcccagcga ttggaaatc gaagtacaa ttcaaaacc 
      ctctacggc aaggacttg atcagcgac ctaaagagc 
      gaattaact ggaaacttc gaaaattta atcgtggca 
      atgatgact ccactcccg caatactac gcgagggag 
      attcacgac gccatcagc ggcgtcgga accgacgaa 
      gacgttctc atcgaaacc atgtgcacc cttagcaat 
      gcggaaatc cgtacaatt cgtgacgcc tatcataga 
      acctactac caaaatctg gagagcgac ttgaagggc 
      gacaccagc ggtcatttc cgccgtctc atggtgtca 
      ctctgcagt gctggacgt gacgaaagt atgcagact 
      aatcctcag gctgcgaca gccgatgcc caggccttg 
      ctccgtgcc ggagagctt caagtcggg acggacgaa 
      tcaacattc aatatgata ctctgtcag aggaatcat 
      gcccaactt cggttgatt ttcgaagag taccagcgc 
      ttaaccggg catgatatt gaaaaagcc atcaagaat 
      gagttttcg ggtgatatt gaagaaggg cttttggcg 
      gtcgtaagg tcgattaag aatcaaccg gcgttcttt 
      gcgaagagg ttgaataaa tcaatgaaa gggatggga 
      acaaatgat cgcgatttg atcagattg gttgtgacg 
      aggtcggag attgatatg ggggatatc aagagggag 
      tatcaggcc aaatatggg gaatcactt gctgatgcc 
      atcaagggc gactgttcc ggtgattat aaaaagtgt 
      cttttggca cttattgga gaatcgtaa
    mRNA sequence
    • >TC011791 RA
      tcttgaaaa ttatgccat aagataatt tttttgctt 
      ttcaactgc ttttattgt ctggtgcaa tggaagttg 
      cataaccat gagtaatca tggtggttt tatcttaaa 
      aaaatgacc tccatttcc attatcgac ccattggta 
      cactgttgg taccaaatt aatttccca ttagagtag 
      atgacacaa ctttcacta taacaagtc acgtgatga 
      gtcgacctt gttttgttt cagtgtgac gttagcaga 
      gaagaagta acttctgaa tcactaaca acgtctgtg 
      aaaattcta attactcac gacacgtca caagtaaaa 
      acaaaatct taatataaa agtgtaatt acaaaatga 
      gttatccag gatatccac cctatcaag cgggctcaa 
      actacccgc cgaacccaa attccgggt caccttatc 
      cccccaatc ccccttatc cccaacaag ggggcgttt 
      atcccccca gtgttgggt atacttcag gctccccca 
      tgtacatgc cgcaaccct acggcggtg gcgggtatc 
      ctcctcaaa ccgggggtt ccccctatc cacaaccta 
      gtccctatc ccagtcaag gggcaggtc cctacccag 
      gtcagggat cctctccct accccaacc agggggcta 
      gtccctatc ctcctccag gtggccaag gagccgctc 
      cttacccca gtcaagggg gccatcaag gaccctctc 
      cttacccct cccaaccct atggccaag gacaccaga 
      cccctagct atacaccct cttatagtc aaggccaac 
      acacaatca agagatccc cgactgtgg ttcccgcgc 
      accctttca accctcgcg aagatgccg aaatcttgc 
      gcaaggcta tgaaaggtt tcggaacgg atgaaaaag 
      ccatcatca atgtcctag cccgccgta ccaacgccc 
      agcgattgg aaatcgaag tacaattca aaaccctct 
      acggcaagg acttgatca gcgacctaa agagcgaat 
      taactggaa acttcgaaa atttaatcg tggcaatga 
      tgactccac tcccgcaat actacgcga gggagattc 
      acgacgcca tcagcggcg tcggaaccg acgaagacg 
      ttctcatcg aaaccatgt gcaccctta gcaatgcgg 
      aaatccgta caattcgtg acgcctatc atagaacct 
      actaccaaa atctggaga gcgacttga agggcgaca 
      ccagcggtc atttccgcc gtctcatgg tgtcactct 
      gcagtgctg gacgtgacg aaagtatgc agactaatc 
      ctcaggctg cgacagccg atgcccagg ccttgctcc 
      gtgccggag agcttcaag tcgggacgg acgaatcaa 
      cattcaata tgatactct gtcagagga atcatgccc 
      aacttcggt tgattttcg aagagtacc agcgcttaa 
      ccgggcatg atattgaaa aagccatca agaatgagt 
      tttcgggtg atattgaag aagggcttt tggcggtcg 
      taaggtcga ttaagaatc aaccggcgt tctttgcga 
      agaggttga ataaatcaa tgaaaggga tgggaacaa 
      atgatcgcg atttgatca gattggttg tgacgaggt 
      cggagattg atatggggg atatcaaga gggagtatc 
      aggccaaat atggggaat cacttgctg atgccatca 
      agggcgact gttccggtg attataaaa agtgtcttt 
      tggcactta ttggagaat cgtaaactg ttaaactta 
      ttgtgtcaa atttttaaa tgtttggtg ctttattcc 
      atgtatttt tatccacat taatgaaat ttatagtac 
      attgttctc tattgttca atatgtatt aaactgtat 
      ttttataat gttattatt aaacttgaa agaatatgg 
      ccaataaag acgagaaaa acgtattct atgagcgaa 
      agttctata tattgtaca atgttgatc tgctattta 
      tgtacacgt ttttgctac tgttataca atttaataa 
      atgtaacgt gcaacatgt tttttcatt tttattccc 
      ccgagttcc atacgcaca tatttacat aattaaaat 
      taaagactt acctttgag cagatcagg aaagcccag 
      aataatctg aaaaaaaaa ac
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