General information | |||
TC034441
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 13479142..13488364 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC034441 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05103 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
oblique dorsal thoracic muscles distalsize shortened - Number of animals showing the phenotype: 8
- >TC034441 RA
MTSLATAVS LLLLLLAEV AGAPATNCK LSEFACNNG RCIPLNKYC NIVNDCGDS TDEPRYCTR CNRTYFGEA GKTYDLELH RPKEDKIPF ICHLTFTAG GDEFGELVQ LTFDSFTLG RFVSFTSDG CPDGALQIT ESNRPQVGG SWCGTSWGP AIYYSETKS VTIYVNLLR LSKDQNGYN FDFRMEYKF LRKKLATVR YGGGQPFVN TTAPSVEPE PEYYLGDLI TGTYCSRIF SDCDRKHCR LQSPNFPGV YPRNLTCYY AVRQHEIPP GKHALIIVR QLKGQLISI RSQSALYGT ASTRELKLW NDCDDVQDY VTIYDGYTT RDPVILKFC GGGEAVPEA VSSGHELLV EFSTSPYGT FLYPAPAQA LHGFQLEVE VKFVDQQSP TYVKTKRQC EFSLRGTNK GVLESPLHS LPANTTCLY HLQGIDTSV SPSPIPFRP LSSRYPDWR HAGMILPPP RYRVWLSIV KFHASGPRD PQKPDQELC RSYLNVWDG QLWAPSNCN DLFCGGRDK SKSIPRTSL SGSSAAKKK NVTLLARYC REHVPRSCD HSLLANSTR FPRPCSLAE SFLTSGDSL TLELKLADS TALRPVQFR ALYEFVDLH LDGEPYGPG PCSRRFSSL NQDGPQKFR SPRDIFLYG RGGAKNISC VYRFEAQKD ERIKITLTS LTIKNRDCK TKLSKDTDR LECFGNTTA TVRLFEIPW MDVPGVPRD CLCSLDKDK LLPFSYVSH GTWKLIQTP VCPSNLRMK GPSGELAFI YPNKSPQET NCENSPRVI IPSPNKYLY VKISGVIMK HSSRLGDGA SRSVSPVRC GTSNRIKVH TALYSALIC PYSSSSRSN LVEVFSEGW SVGNVDGHS VGNMALDKI EIDRLGKEL PRSIVVEFI GKEEGSYSV MWLELGRRR DVPPNGMGL FLLKPDECQ YRCPELDAC INATVWCDG IEDCPSGID EALTHCSII LQLPPLYLF LGACGILIS SFATCMAVW KACRRRPRS ILQTRLKSL SSDTAIIDE KGVIC
- >TC034441 RA
atgacgagc ctagcgact gctgtctct ctgctcctg ctgctgttg gcggaggta gctggcgcg cccgccaca aactgcaaa ctatcggaa ttcgcctgc aacaacggt cgatgcatc cccctcaac aaatactgc aacatcgtg aacgattgc ggagattcg actgacgaa ccgagatat tgcacgcga tgtaatcgc acctatttc ggagaagct ggaaaaacg tacgatttg gaactacat cgtccaaaa gaagacaag ataccgttc atctgtcac ctgacgttc acggctgga ggagacgaa ttcggcgag ctcgtccag ttgacgttc gacagtttc acgttgggt cgtttcgtg tctttcacg tccgacggt tgtccagac ggtgcctta caaataacg gaatccaac aggccccaa gtaggagga tcgtggtgc gggacgtca tggggcccc gccatctat tacagcgaa acgaaaagt gttacgatt tatgttaat cttcttcgt ctttctaaa gatcagaac ggatataac ttcgacttt cgtatggaa tacaagttt ctccggaaa aaactggca actgttcga tacggtgga ggtcagcca ttcgtgaat acaacggcg ccctcagtt gagcccgaa cccgaatac tacttggga gatttaata acgggtaca tattgttcc cgaattttc agcgactgt gataggaag cactgccgt ctccagtcc cccaacttt ccgggggtt tacccgcgc aatttaacc tgttattac gcagtgcgt cagcacgaa attcctccg ggtaaacac gccctcatc attgtacgt caactaaaa ggccaactg atctcaata cgaagccaa tcagctctc tacggtacc gcgagcacc cgcgaactc aaactctgg aacgactgt gacgacgtc caagactac gtcaccatc tacgacggt tacacgacc cgggacccc gtcatttta aaattttgc ggcggcggc gaagccgtc cctgaagcc gtctccagc ggtcacgaa ctcctcgtc gaattctca acgtcgcca tatgggacg ttcctctac ccagcccca gctcaagct ctccacggc ttccagctg gaagttgaa gtcaagttc gtcgatcag caatccccg acttacgtc aaaacgaaa cgccaatgc gagttctct ttgagagga actaacaaa ggggttttg gagagtcct ttgcattcc ttgccggca aacaccacc tgcctctat cacctacaa ggcatcgac acttcagtc tccccatcc ccgatccct ttccggcct ctttcttcc cgttatccc gactggagg cacgccggg atgatcctc ccgccgcct cgttaccgc gtctggctc tccatcgtc aaattccac gcgtctggg cctcgcgac ccgcaaaag cccgaccag gagttgtgc agaagctat ctgaacgtg tgggatgga caactgtgg gcaccctcc aactgcaac gatctcttc tgcggtggc agagataaa tcgaaaagc attcccaga acgagttta tcgggctct tcagccgcc aagaaaaag aacgtgacg ctattggcg cgatactgc agggagcac gtgcctcga agctgcgac cattcactc ctagctaat tcgacgagg ttccctagg ccttgcagc ctcgctgaa agtttcctc acgtcaggg gatagtctc accctggag ttgaagttg gcagactcg acggcactc agacccgtg cagtttcgg gccttgtac gaattcgtc gatttgcac ctcgacggg gaaccctac ggacccgga ccgtgttcc agaaggttc agtagtttg aatcaggat gggcctcag aaatttcgc tcgcccagg gatattttt ctttacggg agaggtgga gcaaaaaac ataagttgc gtgtatcgc tttgaagct cagaaggac gaaaggata aagataact ttaacaagt ctgactatc aaaaatcgc gattgcaag acgaaatta agtaaagac acggatcgt ctcgagtgt tttgggaat acgacagct acagttcgt ctctttgag attccatgg atggacgta ccaggcgtt ccccgcgac tgcctttgc tcgctcgac aaggataaa ctgttaccg ttcagttac gtttcccac ggcacatgg aagttgata caaaccccc gtatgccct agtaattta cggatgaag ggaccgagc ggagaacta gctttcatc tacccgaac aaatcaccg caggaaacc aactgcgag aacagtcct cgtgtgata attccatcg ccaaacaag tatctctac gtgaagatc agcggcgtg ataatgaaa cattcgagc cgactaggc gatggcgca tcgaggagc gtttctccc gtgagatgc ggaacctct aatcggatt aaggtacac acggccctt tattcagct ctcatatgt ccctacagc tcctcgtcg cgaagcaat ctcgttgag gttttctcc gaagggtgg agcgtaggc aacgtcgac ggacactcg gtcggaaac atggcgcta gacaagatc gaaatcgac cgactggga aaagagctc ccgagatcg atcgtcgtc gagtttatt gggaaagaa gagggctcc tactccgtc atgtggctc gaactcggc cgaagacgc gatgtgccg cccaacggc atgggcctc ttcctgctc aagcccgac gaatgccaa taccgctgt ccagagctg gacgcttgc atcaatgcc accgtctgg tgcgacggc atcgaagac tgtccttcc ggcatcgac gaagctctc actcactgc tccataatc ctgcaattg ccgcctctt tatctcttt ttgggcgct tgtgggatc ctcatttcg agcttcgcc acctgtatg gccgtgtgg aaagcttgt cgtcgaaga ccgcgttcg atactccag acgcgtctg aagagtctt tcatctgac actgccatc atcgacgaa aagggagtg atttgctga
- >TC034441 RA
ttaaataaa atcgttgcg tgcacgcat tccccaccc gcaaaaaac ttgttattt agtcgcaaa gtcagaccc aactctctt ataaggctg cgctggcaa atgacgagc ctagcgact gctgtctct ctgctcctg ctgctgttg gcggaggta gctggcgcg cccgccaca aactgcaaa ctatcggaa ttcgcctgc aacaacggt cgatgcatc cccctcaac aaatactgc aacatcgtg aacgattgc ggagattcg actgacgaa ccgagatat tgcacgcga tgtaatcgc acctatttc ggagaagct ggaaaaacg tacgatttg gaactacat cgtccaaaa gaagacaag ataccgttc atctgtcac ctgacgttc acggctgga ggagacgaa ttcggcgag ctcgtccag ttgacgttc gacagtttc acgttgggt cgtttcgtg tctttcacg tccgacggt tgtccagac ggtgcctta caaataacg gaatccaac aggccccaa gtaggagga tcgtggtgc gggacgtca tggggcccc gccatctat tacagcgaa acgaaaagt gttacgatt tatgttaat cttcttcgt ctttctaaa gatcagaac ggatataac ttcgacttt cgtatggaa tacaagttt ctccggaaa aaactggca actgttcga tacggtgga ggtcagcca ttcgtgaat acaacggcg ccctcagtt gagcccgaa cccgaatac tacttggga gatttaata acgggtaca tattgttcc cgaattttc agcgactgt gataggaag cactgccgt ctccagtcc cccaacttt ccgggggtt tacccgcgc aatttaacc tgttattac gcagtgcgt cagcacgaa attcctccg ggtaaacac gccctcatc attgtacgt caactaaaa ggccaactg atctcaata cgaagccaa tcagctctc tacggtacc gcgagcacc cgcgaactc aaactctgg aacgactgt gacgacgtc caagactac gtcaccatc tacgacggt tacacgacc cgggacccc gtcatttta aaattttgc ggcggcggc gaagccgtc cctgaagcc gtctccagc ggtcacgaa ctcctcgtc gaattctca acgtcgcca tatgggacg ttcctctac ccagcccca gctcaagct ctccacggc ttccagctg gaagttgaa gtcaagttc gtcgatcag caatccccg acttacgtc aaaacgaaa cgccaatgc gagttctct ttgagagga actaacaaa ggggttttg gagagtcct ttgcattcc ttgccggca aacaccacc tgcctctat cacctacaa ggcatcgac acttcagtc tccccatcc ccgatccct ttccggcct ctttcttcc cgttatccc gactggagg cacgccggg atgatcctc ccgccgcct cgttaccgc gtctggctc tccatcgtc aaattccac gcgtctggg cctcgcgac ccgcaaaag cccgaccag gagttgtgc agaagctat ctgaacgtg tgggatgga caactgtgg gcaccctcc aactgcaac gatctcttc tgcggtggc agagataaa tcgaaaagc attcccaga acgagttta tcgggctct tcagccgcc aagaaaaag aacgtgacg ctattggcg cgatactgc agggagcac gtgcctcga agctgcgac cattcactc ctagctaat tcgacgagg ttccctagg ccttgcagc ctcgctgaa agtttcctc acgtcaggg gatagtctc accctggag ttgaagttg gcagactcg acggcactc agacccgtg cagtttcgg gccttgtac gaattcgtc gatttgcac ctcgacggg gaaccctac ggacccgga ccgtgttcc agaaggttc agtagtttg aatcaggat gggcctcag aaatttcgc tcgcccagg gatattttt ctttacggg agaggtgga gcaaaaaac ataagttgc gtgtatcgc tttgaagct cagaaggac gaaaggata aagataact ttaacaagt ctgactatc aaaaatcgc gattgcaag acgaaatta agtaaagac acggatcgt ctcgagtgt tttgggaat acgacagct acagttcgt ctctttgag attccatgg atggacgta ccaggcgtt ccccgcgac tgcctttgc tcgctcgac aaggataaa ctgttaccg ttcagttac gtttcccac ggcacatgg aagttgata caaaccccc gtatgccct agtaattta cggatgaag ggaccgagc ggagaacta gctttcatc tacccgaac aaatcaccg caggaaacc aactgcgag aacagtcct cgtgtgata attccatcg ccaaacaag tatctctac gtgaagatc agcggcgtg ataatgaaa cattcgagc cgactaggc gatggcgca tcgaggagc gtttctccc gtgagatgc ggaacctct aatcggatt aaggtacac acggccctt tattcagct ctcatatgt ccctacagc tcctcgtcg cgaagcaat ctcgttgag gttttctcc gaagggtgg agcgtaggc aacgtcgac ggacactcg gtcggaaac atggcgcta gacaagatc gaaatcgac cgactggga aaagagctc ccgagatcg atcgtcgtc gagtttatt gggaaagaa gagggctcc tactccgtc atgtggctc gaactcggc cgaagacgc gatgtgccg cccaacggc atgggcctc ttcctgctc aagcccgac gaatgccaa taccgctgt ccagagctg gacgcttgc atcaatgcc accgtctgg tgcgacggc atcgaagac tgtccttcc ggcatcgac gaagctctc actcactgc tccataatc ctgcaattg ccgcctctt tatctcttt ttgggcgct tgtgggatc ctcatttcg agcttcgcc acctgtatg gccgtgtgg aaagcttgt cgtcgaaga ccgcgttcg atactccag acgcgtctg aagagtctt tcatctgac actgccatc atcgacgaa aagggagtg atttgctga cacagcgac aattctgtg cgttatgtc gaagtcgat cgcgtcaca accgtgtga cacgtcgaa atcgaccta aaacacaga attgataaa cggactttt atacatttc gtaatttag aaacggacc aagcaaatt aagcgaatt tcttagcgt tgtgattac gataggcgt acctggtag aggattgag ggtagggct ttttgcgaa tcgtggagt gagcttttt ttaattaaa acgcattcg ttactaaac acactttta ttttcataa cagaaagga cgtgacgca aatcttttg gcactgtac tgacgagta caatgactg atcacacgt tttgtatat agtacttag gtaaatcaa aaaatttag cgaatttct caaatttgg taggtaata aagagcgta ccgagatta cgtgagtag tcgagcttt acttctatt ttgacaatt tttagtgta atcgaggta acgctgaaa tattttcag ctgaaaata attacgaaa atgcatatc aaatatgct gaaaatgct taaagttca ctgccataa ctgtggcga aaacatcgt tttattctt ttgctcgat ttttcgaaa tgacgactt gagttgaat ttttgttaa acgcacaag ttataaaat tcatatcaa aattttaca ccaaaaaat cgttgattt catttctgc aattgttac gataacatt gtttcaact tttattata gtgtttaaa aacaatatc ttgtagtgt tgttgaaaa aatagaaaa aattcctag taaacacga ttttcattg tacataact tccattaaa aaagtcttt tgaatacgt atatggagc tattttaag tggtagcac aagttggtc aggtgaact gacaataaa gtagatgaa tttaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
