Status
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Gene information
General information
TC034441
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011786,TC011787
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13479142..13488364
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034441
    iBeetle screen iB_05103

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    elytra shortened - Number of animals showing the phenotype: 8

    oblique dorsal thoracic muscles distalsize shortened - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034441 RA
      MTSLATAVS LLLLLLAEV AGAPATNCK LSEFACNNG 
      RCIPLNKYC NIVNDCGDS TDEPRYCTR CNRTYFGEA 
      GKTYDLELH RPKEDKIPF ICHLTFTAG GDEFGELVQ 
      LTFDSFTLG RFVSFTSDG CPDGALQIT ESNRPQVGG 
      SWCGTSWGP AIYYSETKS VTIYVNLLR LSKDQNGYN 
      FDFRMEYKF LRKKLATVR YGGGQPFVN TTAPSVEPE 
      PEYYLGDLI TGTYCSRIF SDCDRKHCR LQSPNFPGV 
      YPRNLTCYY AVRQHEIPP GKHALIIVR QLKGQLISI 
      RSQSALYGT ASTRELKLW NDCDDVQDY VTIYDGYTT 
      RDPVILKFC GGGEAVPEA VSSGHELLV EFSTSPYGT 
      FLYPAPAQA LHGFQLEVE VKFVDQQSP TYVKTKRQC 
      EFSLRGTNK GVLESPLHS LPANTTCLY HLQGIDTSV 
      SPSPIPFRP LSSRYPDWR HAGMILPPP RYRVWLSIV 
      KFHASGPRD PQKPDQELC RSYLNVWDG QLWAPSNCN 
      DLFCGGRDK SKSIPRTSL SGSSAAKKK NVTLLARYC 
      REHVPRSCD HSLLANSTR FPRPCSLAE SFLTSGDSL 
      TLELKLADS TALRPVQFR ALYEFVDLH LDGEPYGPG 
      PCSRRFSSL NQDGPQKFR SPRDIFLYG RGGAKNISC 
      VYRFEAQKD ERIKITLTS LTIKNRDCK TKLSKDTDR 
      LECFGNTTA TVRLFEIPW MDVPGVPRD CLCSLDKDK 
      LLPFSYVSH GTWKLIQTP VCPSNLRMK GPSGELAFI 
      YPNKSPQET NCENSPRVI IPSPNKYLY VKISGVIMK 
      HSSRLGDGA SRSVSPVRC GTSNRIKVH TALYSALIC 
      PYSSSSRSN LVEVFSEGW SVGNVDGHS VGNMALDKI 
      EIDRLGKEL PRSIVVEFI GKEEGSYSV MWLELGRRR 
      DVPPNGMGL FLLKPDECQ YRCPELDAC INATVWCDG 
      IEDCPSGID EALTHCSII LQLPPLYLF LGACGILIS 
      SFATCMAVW KACRRRPRS ILQTRLKSL SSDTAIIDE 
      KGVIC
    CDS sequence
    • >TC034441 RA
      atgacgagc ctagcgact gctgtctct ctgctcctg 
      ctgctgttg gcggaggta gctggcgcg cccgccaca 
      aactgcaaa ctatcggaa ttcgcctgc aacaacggt 
      cgatgcatc cccctcaac aaatactgc aacatcgtg 
      aacgattgc ggagattcg actgacgaa ccgagatat 
      tgcacgcga tgtaatcgc acctatttc ggagaagct 
      ggaaaaacg tacgatttg gaactacat cgtccaaaa 
      gaagacaag ataccgttc atctgtcac ctgacgttc 
      acggctgga ggagacgaa ttcggcgag ctcgtccag 
      ttgacgttc gacagtttc acgttgggt cgtttcgtg 
      tctttcacg tccgacggt tgtccagac ggtgcctta 
      caaataacg gaatccaac aggccccaa gtaggagga 
      tcgtggtgc gggacgtca tggggcccc gccatctat 
      tacagcgaa acgaaaagt gttacgatt tatgttaat 
      cttcttcgt ctttctaaa gatcagaac ggatataac 
      ttcgacttt cgtatggaa tacaagttt ctccggaaa 
      aaactggca actgttcga tacggtgga ggtcagcca 
      ttcgtgaat acaacggcg ccctcagtt gagcccgaa 
      cccgaatac tacttggga gatttaata acgggtaca 
      tattgttcc cgaattttc agcgactgt gataggaag 
      cactgccgt ctccagtcc cccaacttt ccgggggtt 
      tacccgcgc aatttaacc tgttattac gcagtgcgt 
      cagcacgaa attcctccg ggtaaacac gccctcatc 
      attgtacgt caactaaaa ggccaactg atctcaata 
      cgaagccaa tcagctctc tacggtacc gcgagcacc 
      cgcgaactc aaactctgg aacgactgt gacgacgtc 
      caagactac gtcaccatc tacgacggt tacacgacc 
      cgggacccc gtcatttta aaattttgc ggcggcggc 
      gaagccgtc cctgaagcc gtctccagc ggtcacgaa 
      ctcctcgtc gaattctca acgtcgcca tatgggacg 
      ttcctctac ccagcccca gctcaagct ctccacggc 
      ttccagctg gaagttgaa gtcaagttc gtcgatcag 
      caatccccg acttacgtc aaaacgaaa cgccaatgc 
      gagttctct ttgagagga actaacaaa ggggttttg 
      gagagtcct ttgcattcc ttgccggca aacaccacc 
      tgcctctat cacctacaa ggcatcgac acttcagtc 
      tccccatcc ccgatccct ttccggcct ctttcttcc 
      cgttatccc gactggagg cacgccggg atgatcctc 
      ccgccgcct cgttaccgc gtctggctc tccatcgtc 
      aaattccac gcgtctggg cctcgcgac ccgcaaaag 
      cccgaccag gagttgtgc agaagctat ctgaacgtg 
      tgggatgga caactgtgg gcaccctcc aactgcaac 
      gatctcttc tgcggtggc agagataaa tcgaaaagc 
      attcccaga acgagttta tcgggctct tcagccgcc 
      aagaaaaag aacgtgacg ctattggcg cgatactgc 
      agggagcac gtgcctcga agctgcgac cattcactc 
      ctagctaat tcgacgagg ttccctagg ccttgcagc 
      ctcgctgaa agtttcctc acgtcaggg gatagtctc 
      accctggag ttgaagttg gcagactcg acggcactc 
      agacccgtg cagtttcgg gccttgtac gaattcgtc 
      gatttgcac ctcgacggg gaaccctac ggacccgga 
      ccgtgttcc agaaggttc agtagtttg aatcaggat 
      gggcctcag aaatttcgc tcgcccagg gatattttt 
      ctttacggg agaggtgga gcaaaaaac ataagttgc 
      gtgtatcgc tttgaagct cagaaggac gaaaggata 
      aagataact ttaacaagt ctgactatc aaaaatcgc 
      gattgcaag acgaaatta agtaaagac acggatcgt 
      ctcgagtgt tttgggaat acgacagct acagttcgt 
      ctctttgag attccatgg atggacgta ccaggcgtt 
      ccccgcgac tgcctttgc tcgctcgac aaggataaa 
      ctgttaccg ttcagttac gtttcccac ggcacatgg 
      aagttgata caaaccccc gtatgccct agtaattta 
      cggatgaag ggaccgagc ggagaacta gctttcatc 
      tacccgaac aaatcaccg caggaaacc aactgcgag 
      aacagtcct cgtgtgata attccatcg ccaaacaag 
      tatctctac gtgaagatc agcggcgtg ataatgaaa 
      cattcgagc cgactaggc gatggcgca tcgaggagc 
      gtttctccc gtgagatgc ggaacctct aatcggatt 
      aaggtacac acggccctt tattcagct ctcatatgt 
      ccctacagc tcctcgtcg cgaagcaat ctcgttgag 
      gttttctcc gaagggtgg agcgtaggc aacgtcgac 
      ggacactcg gtcggaaac atggcgcta gacaagatc 
      gaaatcgac cgactggga aaagagctc ccgagatcg 
      atcgtcgtc gagtttatt gggaaagaa gagggctcc 
      tactccgtc atgtggctc gaactcggc cgaagacgc 
      gatgtgccg cccaacggc atgggcctc ttcctgctc 
      aagcccgac gaatgccaa taccgctgt ccagagctg 
      gacgcttgc atcaatgcc accgtctgg tgcgacggc 
      atcgaagac tgtccttcc ggcatcgac gaagctctc 
      actcactgc tccataatc ctgcaattg ccgcctctt 
      tatctcttt ttgggcgct tgtgggatc ctcatttcg 
      agcttcgcc acctgtatg gccgtgtgg aaagcttgt 
      cgtcgaaga ccgcgttcg atactccag acgcgtctg 
      aagagtctt tcatctgac actgccatc atcgacgaa 
      aagggagtg atttgctga
    mRNA sequence
    • >TC034441 RA
      ttaaataaa atcgttgcg tgcacgcat tccccaccc 
      gcaaaaaac ttgttattt agtcgcaaa gtcagaccc 
      aactctctt ataaggctg cgctggcaa atgacgagc 
      ctagcgact gctgtctct ctgctcctg ctgctgttg 
      gcggaggta gctggcgcg cccgccaca aactgcaaa 
      ctatcggaa ttcgcctgc aacaacggt cgatgcatc 
      cccctcaac aaatactgc aacatcgtg aacgattgc 
      ggagattcg actgacgaa ccgagatat tgcacgcga 
      tgtaatcgc acctatttc ggagaagct ggaaaaacg 
      tacgatttg gaactacat cgtccaaaa gaagacaag 
      ataccgttc atctgtcac ctgacgttc acggctgga 
      ggagacgaa ttcggcgag ctcgtccag ttgacgttc 
      gacagtttc acgttgggt cgtttcgtg tctttcacg 
      tccgacggt tgtccagac ggtgcctta caaataacg 
      gaatccaac aggccccaa gtaggagga tcgtggtgc 
      gggacgtca tggggcccc gccatctat tacagcgaa 
      acgaaaagt gttacgatt tatgttaat cttcttcgt 
      ctttctaaa gatcagaac ggatataac ttcgacttt 
      cgtatggaa tacaagttt ctccggaaa aaactggca 
      actgttcga tacggtgga ggtcagcca ttcgtgaat 
      acaacggcg ccctcagtt gagcccgaa cccgaatac 
      tacttggga gatttaata acgggtaca tattgttcc 
      cgaattttc agcgactgt gataggaag cactgccgt 
      ctccagtcc cccaacttt ccgggggtt tacccgcgc 
      aatttaacc tgttattac gcagtgcgt cagcacgaa 
      attcctccg ggtaaacac gccctcatc attgtacgt 
      caactaaaa ggccaactg atctcaata cgaagccaa 
      tcagctctc tacggtacc gcgagcacc cgcgaactc 
      aaactctgg aacgactgt gacgacgtc caagactac 
      gtcaccatc tacgacggt tacacgacc cgggacccc 
      gtcatttta aaattttgc ggcggcggc gaagccgtc 
      cctgaagcc gtctccagc ggtcacgaa ctcctcgtc 
      gaattctca acgtcgcca tatgggacg ttcctctac 
      ccagcccca gctcaagct ctccacggc ttccagctg 
      gaagttgaa gtcaagttc gtcgatcag caatccccg 
      acttacgtc aaaacgaaa cgccaatgc gagttctct 
      ttgagagga actaacaaa ggggttttg gagagtcct 
      ttgcattcc ttgccggca aacaccacc tgcctctat 
      cacctacaa ggcatcgac acttcagtc tccccatcc 
      ccgatccct ttccggcct ctttcttcc cgttatccc 
      gactggagg cacgccggg atgatcctc ccgccgcct 
      cgttaccgc gtctggctc tccatcgtc aaattccac 
      gcgtctggg cctcgcgac ccgcaaaag cccgaccag 
      gagttgtgc agaagctat ctgaacgtg tgggatgga 
      caactgtgg gcaccctcc aactgcaac gatctcttc 
      tgcggtggc agagataaa tcgaaaagc attcccaga 
      acgagttta tcgggctct tcagccgcc aagaaaaag 
      aacgtgacg ctattggcg cgatactgc agggagcac 
      gtgcctcga agctgcgac cattcactc ctagctaat 
      tcgacgagg ttccctagg ccttgcagc ctcgctgaa 
      agtttcctc acgtcaggg gatagtctc accctggag 
      ttgaagttg gcagactcg acggcactc agacccgtg 
      cagtttcgg gccttgtac gaattcgtc gatttgcac 
      ctcgacggg gaaccctac ggacccgga ccgtgttcc 
      agaaggttc agtagtttg aatcaggat gggcctcag 
      aaatttcgc tcgcccagg gatattttt ctttacggg 
      agaggtgga gcaaaaaac ataagttgc gtgtatcgc 
      tttgaagct cagaaggac gaaaggata aagataact 
      ttaacaagt ctgactatc aaaaatcgc gattgcaag 
      acgaaatta agtaaagac acggatcgt ctcgagtgt 
      tttgggaat acgacagct acagttcgt ctctttgag 
      attccatgg atggacgta ccaggcgtt ccccgcgac 
      tgcctttgc tcgctcgac aaggataaa ctgttaccg 
      ttcagttac gtttcccac ggcacatgg aagttgata 
      caaaccccc gtatgccct agtaattta cggatgaag 
      ggaccgagc ggagaacta gctttcatc tacccgaac 
      aaatcaccg caggaaacc aactgcgag aacagtcct 
      cgtgtgata attccatcg ccaaacaag tatctctac 
      gtgaagatc agcggcgtg ataatgaaa cattcgagc 
      cgactaggc gatggcgca tcgaggagc gtttctccc 
      gtgagatgc ggaacctct aatcggatt aaggtacac 
      acggccctt tattcagct ctcatatgt ccctacagc 
      tcctcgtcg cgaagcaat ctcgttgag gttttctcc 
      gaagggtgg agcgtaggc aacgtcgac ggacactcg 
      gtcggaaac atggcgcta gacaagatc gaaatcgac 
      cgactggga aaagagctc ccgagatcg atcgtcgtc 
      gagtttatt gggaaagaa gagggctcc tactccgtc 
      atgtggctc gaactcggc cgaagacgc gatgtgccg 
      cccaacggc atgggcctc ttcctgctc aagcccgac 
      gaatgccaa taccgctgt ccagagctg gacgcttgc 
      atcaatgcc accgtctgg tgcgacggc atcgaagac 
      tgtccttcc ggcatcgac gaagctctc actcactgc 
      tccataatc ctgcaattg ccgcctctt tatctcttt 
      ttgggcgct tgtgggatc ctcatttcg agcttcgcc 
      acctgtatg gccgtgtgg aaagcttgt cgtcgaaga 
      ccgcgttcg atactccag acgcgtctg aagagtctt 
      tcatctgac actgccatc atcgacgaa aagggagtg 
      atttgctga cacagcgac aattctgtg cgttatgtc 
      gaagtcgat cgcgtcaca accgtgtga cacgtcgaa 
      atcgaccta aaacacaga attgataaa cggactttt 
      atacatttc gtaatttag aaacggacc aagcaaatt 
      aagcgaatt tcttagcgt tgtgattac gataggcgt 
      acctggtag aggattgag ggtagggct ttttgcgaa 
      tcgtggagt gagcttttt ttaattaaa acgcattcg 
      ttactaaac acactttta ttttcataa cagaaagga 
      cgtgacgca aatcttttg gcactgtac tgacgagta 
      caatgactg atcacacgt tttgtatat agtacttag 
      gtaaatcaa aaaatttag cgaatttct caaatttgg 
      taggtaata aagagcgta ccgagatta cgtgagtag 
      tcgagcttt acttctatt ttgacaatt tttagtgta 
      atcgaggta acgctgaaa tattttcag ctgaaaata 
      attacgaaa atgcatatc aaatatgct gaaaatgct 
      taaagttca ctgccataa ctgtggcga aaacatcgt 
      tttattctt ttgctcgat ttttcgaaa tgacgactt 
      gagttgaat ttttgttaa acgcacaag ttataaaat 
      tcatatcaa aattttaca ccaaaaaat cgttgattt 
      catttctgc aattgttac gataacatt gtttcaact 
      tttattata gtgtttaaa aacaatatc ttgtagtgt 
      tgttgaaaa aatagaaaa aattcctag taaacacga 
      ttttcattg tacataact tccattaaa aaagtcttt 
      tgaatacgt atatggagc tattttaag tggtagcac 
      aagttggtc aggtgaact gacaataaa gtagatgaa 
      tttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY