Status
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Gene information
General information
TC034441
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011786,TC011787 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13479142..13488364
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034441
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05103

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    Pupal morphology
    elytra shortened - Number of animals showing the phenotype: 8

    oblique dorsal thoracic muscles distalsize shortened - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034441 RA
      MTSLATAVS LLLLLLAEV AGAPATNCK LSEFACNNG 
RCIPLNKYC NIVNDCGDS TDEPRYCTR CNRTYFGEA 
GKTYDLELH RPKEDKIPF ICHLTFTAG GDEFGELVQ 
LTFDSFTLG RFVSFTSDG CPDGALQIT ESNRPQVGG 
SWCGTSWGP AIYYSETKS VTIYVNLLR LSKDQNGYN 
FDFRMEYKF LRKKLATVR YGGGQPFVN TTAPSVEPE 
PEYYLGDLI TGTYCSRIF SDCDRKHCR LQSPNFPGV 
YPRNLTCYY AVRQHEIPP GKHALIIVR QLKGQLISI 
RSQSALYGT ASTRELKLW NDCDDVQDY VTIYDGYTT 
RDPVILKFC GGGEAVPEA VSSGHELLV EFSTSPYGT 
FLYPAPAQA LHGFQLEVE VKFVDQQSP TYVKTKRQC 
EFSLRGTNK GVLESPLHS LPANTTCLY HLQGIDTSV 
SPSPIPFRP LSSRYPDWR HAGMILPPP RYRVWLSIV 
KFHASGPRD PQKPDQELC RSYLNVWDG QLWAPSNCN 
DLFCGGRDK SKSIPRTSL SGSSAAKKK NVTLLARYC 
REHVPRSCD HSLLANSTR FPRPCSLAE SFLTSGDSL 
TLELKLADS TALRPVQFR ALYEFVDLH LDGEPYGPG 
PCSRRFSSL NQDGPQKFR SPRDIFLYG RGGAKNISC 
VYRFEAQKD ERIKITLTS LTIKNRDCK TKLSKDTDR 
LECFGNTTA TVRLFEIPW MDVPGVPRD CLCSLDKDK 
LLPFSYVSH GTWKLIQTP VCPSNLRMK GPSGELAFI 
YPNKSPQET NCENSPRVI IPSPNKYLY VKISGVIMK 
HSSRLGDGA SRSVSPVRC GTSNRIKVH TALYSALIC 
PYSSSSRSN LVEVFSEGW SVGNVDGHS VGNMALDKI 
EIDRLGKEL PRSIVVEFI GKEEGSYSV MWLELGRRR 
DVPPNGMGL FLLKPDECQ YRCPELDAC INATVWCDG 
IEDCPSGID EALTHCSII LQLPPLYLF LGACGILIS 
SFATCMAVW KACRRRPRS ILQTRLKSL SSDTAIIDE 
KGVIC
    CDS sequence
    • >TC034441 RA
      atgacgagc ctagcgact gctgtctct ctgctcctg 
ctgctgttg gcggaggta gctggcgcg cccgccaca 
aactgcaaa ctatcggaa ttcgcctgc aacaacggt 
cgatgcatc cccctcaac aaatactgc aacatcgtg 
aacgattgc ggagattcg actgacgaa ccgagatat 
tgcacgcga tgtaatcgc acctatttc ggagaagct 
ggaaaaacg tacgatttg gaactacat cgtccaaaa 
gaagacaag ataccgttc atctgtcac ctgacgttc 
acggctgga ggagacgaa ttcggcgag ctcgtccag 
ttgacgttc gacagtttc acgttgggt cgtttcgtg 
tctttcacg tccgacggt tgtccagac ggtgcctta 
caaataacg gaatccaac aggccccaa gtaggagga 
tcgtggtgc gggacgtca tggggcccc gccatctat 
tacagcgaa acgaaaagt gttacgatt tatgttaat 
cttcttcgt ctttctaaa gatcagaac ggatataac 
ttcgacttt cgtatggaa tacaagttt ctccggaaa 
aaactggca actgttcga tacggtgga ggtcagcca 
ttcgtgaat acaacggcg ccctcagtt gagcccgaa 
cccgaatac tacttggga gatttaata acgggtaca 
tattgttcc cgaattttc agcgactgt gataggaag 
cactgccgt ctccagtcc cccaacttt ccgggggtt 
tacccgcgc aatttaacc tgttattac gcagtgcgt 
cagcacgaa attcctccg ggtaaacac gccctcatc 
attgtacgt caactaaaa ggccaactg atctcaata 
cgaagccaa tcagctctc tacggtacc gcgagcacc 
cgcgaactc aaactctgg aacgactgt gacgacgtc 
caagactac gtcaccatc tacgacggt tacacgacc 
cgggacccc gtcatttta aaattttgc ggcggcggc 
gaagccgtc cctgaagcc gtctccagc ggtcacgaa 
ctcctcgtc gaattctca acgtcgcca tatgggacg 
ttcctctac ccagcccca gctcaagct ctccacggc 
ttccagctg gaagttgaa gtcaagttc gtcgatcag 
caatccccg acttacgtc aaaacgaaa cgccaatgc 
gagttctct ttgagagga actaacaaa ggggttttg 
gagagtcct ttgcattcc ttgccggca aacaccacc 
tgcctctat cacctacaa ggcatcgac acttcagtc 
tccccatcc ccgatccct ttccggcct ctttcttcc 
cgttatccc gactggagg cacgccggg atgatcctc 
ccgccgcct cgttaccgc gtctggctc tccatcgtc 
aaattccac gcgtctggg cctcgcgac ccgcaaaag 
cccgaccag gagttgtgc agaagctat ctgaacgtg 
tgggatgga caactgtgg gcaccctcc aactgcaac 
gatctcttc tgcggtggc agagataaa tcgaaaagc 
attcccaga acgagttta tcgggctct tcagccgcc 
aagaaaaag aacgtgacg ctattggcg cgatactgc 
agggagcac gtgcctcga agctgcgac cattcactc 
ctagctaat tcgacgagg ttccctagg ccttgcagc 
ctcgctgaa agtttcctc acgtcaggg gatagtctc 
accctggag ttgaagttg gcagactcg acggcactc 
agacccgtg cagtttcgg gccttgtac gaattcgtc 
gatttgcac ctcgacggg gaaccctac ggacccgga 
ccgtgttcc agaaggttc agtagtttg aatcaggat 
gggcctcag aaatttcgc tcgcccagg gatattttt 
ctttacggg agaggtgga gcaaaaaac ataagttgc 
gtgtatcgc tttgaagct cagaaggac gaaaggata 
aagataact ttaacaagt ctgactatc aaaaatcgc 
gattgcaag acgaaatta agtaaagac acggatcgt 
ctcgagtgt tttgggaat acgacagct acagttcgt 
ctctttgag attccatgg atggacgta ccaggcgtt 
ccccgcgac tgcctttgc tcgctcgac aaggataaa 
ctgttaccg ttcagttac gtttcccac ggcacatgg 
aagttgata caaaccccc gtatgccct agtaattta 
cggatgaag ggaccgagc ggagaacta gctttcatc 
tacccgaac aaatcaccg caggaaacc aactgcgag 
aacagtcct cgtgtgata attccatcg ccaaacaag 
tatctctac gtgaagatc agcggcgtg ataatgaaa 
cattcgagc cgactaggc gatggcgca tcgaggagc 
gtttctccc gtgagatgc ggaacctct aatcggatt 
aaggtacac acggccctt tattcagct ctcatatgt 
ccctacagc tcctcgtcg cgaagcaat ctcgttgag 
gttttctcc gaagggtgg agcgtaggc aacgtcgac 
ggacactcg gtcggaaac atggcgcta gacaagatc 
gaaatcgac cgactggga aaagagctc ccgagatcg 
atcgtcgtc gagtttatt gggaaagaa gagggctcc 
tactccgtc atgtggctc gaactcggc cgaagacgc 
gatgtgccg cccaacggc atgggcctc ttcctgctc 
aagcccgac gaatgccaa taccgctgt ccagagctg 
gacgcttgc atcaatgcc accgtctgg tgcgacggc 
atcgaagac tgtccttcc ggcatcgac gaagctctc 
actcactgc tccataatc ctgcaattg ccgcctctt 
tatctcttt ttgggcgct tgtgggatc ctcatttcg 
agcttcgcc acctgtatg gccgtgtgg aaagcttgt 
cgtcgaaga ccgcgttcg atactccag acgcgtctg 
aagagtctt tcatctgac actgccatc atcgacgaa 
aagggagtg atttgctga
    mRNA sequence
    • >TC034441 RA
      ttaaataaa atcgttgcg tgcacgcat tccccaccc 
gcaaaaaac ttgttattt agtcgcaaa gtcagaccc 
aactctctt ataaggctg cgctggcaa atgacgagc 
ctagcgact gctgtctct ctgctcctg ctgctgttg 
gcggaggta gctggcgcg cccgccaca aactgcaaa 
ctatcggaa ttcgcctgc aacaacggt cgatgcatc 
cccctcaac aaatactgc aacatcgtg aacgattgc 
ggagattcg actgacgaa ccgagatat tgcacgcga 
tgtaatcgc acctatttc ggagaagct ggaaaaacg 
tacgatttg gaactacat cgtccaaaa gaagacaag 
ataccgttc atctgtcac ctgacgttc acggctgga 
ggagacgaa ttcggcgag ctcgtccag ttgacgttc 
gacagtttc acgttgggt cgtttcgtg tctttcacg 
tccgacggt tgtccagac ggtgcctta caaataacg 
gaatccaac aggccccaa gtaggagga tcgtggtgc 
gggacgtca tggggcccc gccatctat tacagcgaa 
acgaaaagt gttacgatt tatgttaat cttcttcgt 
ctttctaaa gatcagaac ggatataac ttcgacttt 
cgtatggaa tacaagttt ctccggaaa aaactggca 
actgttcga tacggtgga ggtcagcca ttcgtgaat 
acaacggcg ccctcagtt gagcccgaa cccgaatac 
tacttggga gatttaata acgggtaca tattgttcc 
cgaattttc agcgactgt gataggaag cactgccgt 
ctccagtcc cccaacttt ccgggggtt tacccgcgc 
aatttaacc tgttattac gcagtgcgt cagcacgaa 
attcctccg ggtaaacac gccctcatc attgtacgt 
caactaaaa ggccaactg atctcaata cgaagccaa 
tcagctctc tacggtacc gcgagcacc cgcgaactc 
aaactctgg aacgactgt gacgacgtc caagactac 
gtcaccatc tacgacggt tacacgacc cgggacccc 
gtcatttta aaattttgc ggcggcggc gaagccgtc 
cctgaagcc gtctccagc ggtcacgaa ctcctcgtc 
gaattctca acgtcgcca tatgggacg ttcctctac 
ccagcccca gctcaagct ctccacggc ttccagctg 
gaagttgaa gtcaagttc gtcgatcag caatccccg 
acttacgtc aaaacgaaa cgccaatgc gagttctct 
ttgagagga actaacaaa ggggttttg gagagtcct 
ttgcattcc ttgccggca aacaccacc tgcctctat 
cacctacaa ggcatcgac acttcagtc tccccatcc 
ccgatccct ttccggcct ctttcttcc cgttatccc 
gactggagg cacgccggg atgatcctc ccgccgcct 
cgttaccgc gtctggctc tccatcgtc aaattccac 
gcgtctggg cctcgcgac ccgcaaaag cccgaccag 
gagttgtgc agaagctat ctgaacgtg tgggatgga 
caactgtgg gcaccctcc aactgcaac gatctcttc 
tgcggtggc agagataaa tcgaaaagc attcccaga 
acgagttta tcgggctct tcagccgcc aagaaaaag 
aacgtgacg ctattggcg cgatactgc agggagcac 
gtgcctcga agctgcgac cattcactc ctagctaat 
tcgacgagg ttccctagg ccttgcagc ctcgctgaa 
agtttcctc acgtcaggg gatagtctc accctggag 
ttgaagttg gcagactcg acggcactc agacccgtg 
cagtttcgg gccttgtac gaattcgtc gatttgcac 
ctcgacggg gaaccctac ggacccgga ccgtgttcc 
agaaggttc agtagtttg aatcaggat gggcctcag 
aaatttcgc tcgcccagg gatattttt ctttacggg 
agaggtgga gcaaaaaac ataagttgc gtgtatcgc 
tttgaagct cagaaggac gaaaggata aagataact 
ttaacaagt ctgactatc aaaaatcgc gattgcaag 
acgaaatta agtaaagac acggatcgt ctcgagtgt 
tttgggaat acgacagct acagttcgt ctctttgag 
attccatgg atggacgta ccaggcgtt ccccgcgac 
tgcctttgc tcgctcgac aaggataaa ctgttaccg 
ttcagttac gtttcccac ggcacatgg aagttgata 
caaaccccc gtatgccct agtaattta cggatgaag 
ggaccgagc ggagaacta gctttcatc tacccgaac 
aaatcaccg caggaaacc aactgcgag aacagtcct 
cgtgtgata attccatcg ccaaacaag tatctctac 
gtgaagatc agcggcgtg ataatgaaa cattcgagc 
cgactaggc gatggcgca tcgaggagc gtttctccc 
gtgagatgc ggaacctct aatcggatt aaggtacac 
acggccctt tattcagct ctcatatgt ccctacagc 
tcctcgtcg cgaagcaat ctcgttgag gttttctcc 
gaagggtgg agcgtaggc aacgtcgac ggacactcg 
gtcggaaac atggcgcta gacaagatc gaaatcgac 
cgactggga aaagagctc ccgagatcg atcgtcgtc 
gagtttatt gggaaagaa gagggctcc tactccgtc 
atgtggctc gaactcggc cgaagacgc gatgtgccg 
cccaacggc atgggcctc ttcctgctc aagcccgac 
gaatgccaa taccgctgt ccagagctg gacgcttgc 
atcaatgcc accgtctgg tgcgacggc atcgaagac 
tgtccttcc ggcatcgac gaagctctc actcactgc 
tccataatc ctgcaattg ccgcctctt tatctcttt 
ttgggcgct tgtgggatc ctcatttcg agcttcgcc 
acctgtatg gccgtgtgg aaagcttgt cgtcgaaga 
ccgcgttcg atactccag acgcgtctg aagagtctt 
tcatctgac actgccatc atcgacgaa aagggagtg 
atttgctga cacagcgac aattctgtg cgttatgtc 
gaagtcgat cgcgtcaca accgtgtga cacgtcgaa 
atcgaccta aaacacaga attgataaa cggactttt 
atacatttc gtaatttag aaacggacc aagcaaatt 
aagcgaatt tcttagcgt tgtgattac gataggcgt 
acctggtag aggattgag ggtagggct ttttgcgaa 
tcgtggagt gagcttttt ttaattaaa acgcattcg 
ttactaaac acactttta ttttcataa cagaaagga 
cgtgacgca aatcttttg gcactgtac tgacgagta 
caatgactg atcacacgt tttgtatat agtacttag 
gtaaatcaa aaaatttag cgaatttct caaatttgg 
taggtaata aagagcgta ccgagatta cgtgagtag 
tcgagcttt acttctatt ttgacaatt tttagtgta 
atcgaggta acgctgaaa tattttcag ctgaaaata 
attacgaaa atgcatatc aaatatgct gaaaatgct 
taaagttca ctgccataa ctgtggcga aaacatcgt 
tttattctt ttgctcgat ttttcgaaa tgacgactt 
gagttgaat ttttgttaa acgcacaag ttataaaat 
tcatatcaa aattttaca ccaaaaaat cgttgattt 
catttctgc aattgttac gataacatt gtttcaact 
tttattata gtgtttaaa aacaatatc ttgtagtgt 
tgttgaaaa aatagaaaa aattcctag taaacacga 
ttttcattg tacataact tccattaaa aaagtcttt 
tgaatacgt atatggagc tattttaag tggtagcac 
aagttggtc aggtgaact gacaataaa gtagatgaa 
tttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY