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Gene information
General information
TC011771 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13724341..13728149
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011771
    iBeetle screen iB_05101

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011771 RA
      MYEQDEDQF DDEESEEIS SELWQEACW IVINAYFDE 
      KGLVRQQLD SFDEFIQMS VQRIVEDSP QIDLQAEAQ 
      HTSGELENP SRYLLKFEQ IYLSKPTHW EKDGAPSPM 
      MPNEARLRN LTYSAPLYV DITKTIVKD GEDPIETQH 
      QKTFIGKIP IMLRSTYCL LNGLTDRDL TELNECPLD 
      PGGYFIING SEKVLIAQE KMATNTVYV FSMKDGKYA 
      FKSEIRSCL EHSSRPTST LWVNMMARG GQAIKKAAI 
      GQRIIAILP YIKQEIPIM IVFRALGFV ADRDILEHI 
      IYDFEDPEM MEMVKPSLD EAFVIQEQN IALNFIGTR 
      GARPGVTKE KRIKYAREI LQKEMLPHV GVSDFCETK 
      KAYFLGYMV HRLLLAALG RRELDDRDH YGNKRLDLA 
      GPLLAFLFR GLFKNLMKE VRMYAQKFI DRGKDFNLD 
      LAIKTKLIT DGLRYSLAT GNWGDQKKA HQARAGVSQ 
      VLNRLTFAS TLSHLRRVN SPIGRDGKL AKPRQLHNT 
      LWGMICPAE TPEGAAVGL VKNLALMAY ISVGSQPSP 
      ILEFLEEWS MENLEEIAP SAIANATKI FVNGCWVGI 
      HRDPEQLMA TLRKLRRQM DIIVSEVSM IRDIRDREI 
      RIYTDAGRI CRPLLIVEN GTLLLKKRH IDMLKEREY 
      NNYGWQVLV ASGVVEYID TLEEETVMI AMSPDDLRQ 
      EKEYAYCTT YTHCEIHPA MILGVCASI IPFPDHNQS 
      PRNTYQSAM GKQAMGVYI TNFHVRMDT LAHVLYYPH 
      KPLVTTRSM EYLRFRELP AGINSIVAI ACYTGYNQE 
      DSVILNASA VERGFFRSV FYRSYKDAE SKRIGDQEE 
      QFEKPTRQT CQGMRNALY DKLDDDGII APGIRVSGD 
      DVVIGKTMT LPENDDELD GTTKRFTKR DASTFLRNS 
      ETGVVDQVM LTLNSEGYK FCKIRVRSV RIPQIGDKF 
      ASRHGQKGT CGIQYRQED MIFTCEGLT PDIIINPHA 
      IPSRMTIGH LIECIQGKV SANKGEIGD ATPFNDAVN 
      VQKISTLLQ EYGYQLRGN EVMYNGHTG RKINAQIFL 
      GPTYYQRLK HMVDDKIHS RARGPVQIL VRQPMEGRA 
      RDGGLRFGE MERDCQISH GAAQFLRER LFEVSDQYR 
      IHVCNFCGL IAIANMRNN TFECKGCKN KTQISQVRL 
      PYAAKLLFQ ELMSMNIAP RLMVL
    CDS sequence
    • >TC011771 RA
      atgtatgaa caagacgaa gaccaattc gacgatgaa 
      gagtctgaa gaaatcagc tccgagttg tggcaagag 
      gcctgctgg atcgtcatc aacgcctat ttcgacgaa 
      aaaggcctc gtccggcaa caactggac agtttcgac 
      gagtttatc caaatgtcg gtgcaacga atcgtcgaa 
      gactcgccc caaatcgac ctccaagcc gaggcacag 
      cacacaagc ggcgagctt gaaaacccc tctcggtat 
      ttactgaaa ttcgagcag atctaccta tccaaaccg 
      acccattgg gagaaagac ggcgccccc tcgcccatg 
      atgcccaac gaggcccgc ttgcgcaac ctgacctac 
      tcggccccc ttgtacgtc gatatcacc aagaccata 
      gtcaaggac ggcgaggac cccattgag acccagcac 
      cagaaaacc ttcatcggg aaaatcccg attatgctc 
      cgatccacg tactgtctc ctcaacggt ctaaccgac 
      cgcgacttg accgaactc aacgagtgc ccccttgac 
      cccggcggc tacttcatc atcaacggc tcagagaaa 
      gtcctaatc gcccaggag aaaatggcc acgaacacc 
      gtctacgtc ttctcgatg aaagacggg aaatacgcc 
      ttcaaatcc gaaatccgc tcctgtctc gagcacagc 
      tcgcgcccc acgtccacc ctctgggtc aacatgatg 
      gcacgtggg ggccaggcg atcaaaaag gccgccatc 
      gggcaacga atcatcgcg atcttgccc tacatcaag 
      caggagatc cccatcatg atcgtgttc cgggccttg 
      gggttcgtg gccgatcgg gacatcctc gagcacatt 
      atttacgac ttcgaagac cccgagatg atggaaatg 
      gtgaaaccg tccctggac gaagcgttc gtgatccag 
      gaacagaac atcgcactc aacttcatt gggaccagg 
      ggggcccgg ccgggcgtc accaaagaa aagcgtatc 
      aaatacgcg cgagagatc ctccagaag gagatgctg 
      ccccacgtt ggagtgtcc gatttttgc gagacgaaa 
      aaagcctac tttttgggg tacatggtg caccggttg 
      ctcctggcg gctttgggg cgccgcgag ctggacgat 
      cgggatcac tacggtaac aaacgactg gacttggcg 
      gggcccctg cttgcgttt ttgttccgt gggttgttt 
      aaaaacttg atgaaggaa gtacggatg tatgcccag 
      aaatttatt gatcggggc aaagacttt aacctcgac 
      ttggccatt aagactaag ttgattacg gacgggttg 
      aggtattct ctggcgact ggtaactgg ggggaccag 
      aagaaggcc caccaggcc agagccggt gtctcgcag 
      gtgttgaac cgtttgact tttgcctcg actttgtcg 
      cacttgaga cgtgttaat tcgcccatt ggacgtgac 
      gggaagttg gcgaagccc cgacagttg cacaacacg 
      ctctggggg atgatttgt ccggcggag acaccagaa 
      ggggcggct gttggtctg gtgaaaaat ctggccctc 
      atggcgtat atttcggtg gggtcgcaa ccctctcca 
      attttggag tttttggag gagtggtcg atggagaat 
      ttggaggag attgcgccg tctgccata gccaacgcg 
      accaagatt tttgttaat ggttgctgg gtgggaatt 
      cacagggac cccgaacag ttgatggcg acgttacgc 
      aagttgagg agacagatg gatattatt gtgtcggag 
      gtttccatg attcgggat attcgggac cgggagatt 
      cgcatttac acggatgct ggacggatt tgtcggccg 
      ttactaatc gtggaaaat gggacttta ttgttgaag 
      aagcgccac attgatatg ttgaaagag agggaatat 
      aataactac gggtggcag gttttggtg gcttcaggg 
      gttgtcgaa tatatcgat actttggaa gaggagact 
      gtgatgatt gcgatgtct ccggacgat ttgagacag 
      gagaaggaa tacgcgtat tgtaccact tacacacat 
      tgtgagatc catccagct atgattttg ggtgtttgt 
      gccagtatt atacccttc ccggatcac aaccagagt 
      cccagaaac acatatcaa agtgctatg ggcaagcaa 
      gccatgggt gtgtacatc accaacttc catgtccgc 
      atggacacc cttgcccac gtcctgtat tacccccac 
      aaaccgttg gttacaacc agatcaatg gaatatctc 
      agattcagg gaattaccg gctgggatt aacagcatc 
      gtggccatt gcgtgttac accggttat aaccaggag 
      gattcggtt attttgaac gcgtcggcc gtcgaaagg 
      ggatttttc cgatctgtg ttttaccgc tcgtacaag 
      gacgccgaa tcgaaacgc atcggcgac caagaggag 
      cagttcgag aaaccgact cgccagacc tgccaaggc 
      atgcgcaac gccctctac gacaaactg gacgatgac 
      ggcatcata gcaccagga atccgtgtc tctggcgac 
      gacgtcgtc atcggcaaa acaatgaca ctgccggaa 
      aacgacgac gagttggac ggcaccacc aaacgtttc 
      acaaaacgt gacgcctcc acgttcctc cggaacagc 
      gaaacgggg gtggtggac caggtcatg ctcaccctc 
      aactcggag ggctacaaa ttctgcaaa attagggtg 
      cgttccgtg cgcatcccc caaattggg gacaagttc 
      gcgtcacgg cacggacag aagggcacc tgcgggata 
      cagtaccgc caggaggac atgattttc acgtgcgag 
      ggcctaacc cctgacatt ataatcaac ccccatgcc 
      atcccctcg cgtatgacc atcgggcac ttgattgag 
      tgcatccaa ggcaaagtc tcggcaaac aagggcgaa 
      atcggcgac gctaccccg tttaatgac gccgtcaac 
      gtgcagaaa atctcaact ttgctacaa gagtacggg 
      tatcagctc cgaggcaac gaagtgatg tacaatgga 
      cacacaggc cggaaaatc aacgcgcag attttctta 
      ggcccgacg tattatcag agactgaag cacatggtc 
      gacgataag atacattcg agggcgcga gggcccgtc 
      cagattttg gtgcgacaa cccatggaa gggagggcc 
      cgcgatggg gggctgcgg tttggggaa atggaacgt 
      gattgtcag atttcgcac ggagcggca cagtttcta 
      cgcgagagg ttgttcgag gtttcggat cagtataga 
      atacatgtt tgtaatttt tgcgggttg attgccatc 
      gctaatatg aggaataac acgttcgag tgcaagggg 
      tgcaaaaat aagacgcag atttcgcaa gtgaggctg 
      ccgtatgcg gccaagttg ctcttccag gagctgatg 
      tcgatgaat atcgcgccg cggttgatg gttttgtaa
    mRNA sequence
    • >TC011771 RA
      actgacact tgacagacg ctcaaaact tgtgttttg 
      tgcgtctca tttgtgccc cattttttg caaataatc 
      agcaatgta tgaacaaga cgaagacca attcgacga 
      tgaagagtc tgaagaaat cagctccga gttgtggca 
      agaggcctg ctggatcgt catcaacgc ctatttcga 
      cgaaaaagg cctcgtccg gcaacaact ggacagttt 
      cgacgagtt tatccaaat gtcggtgca acgaatcgt 
      cgaagactc gccccaaat cgacctcca agccgaggc 
      acagcacac aagcggcga gcttgaaaa cccctctcg 
      gtatttact gaaattcga gcagatcta cctatccaa 
      accgaccca ttgggagaa agacggcgc cccctcgcc 
      catgatgcc caacgaggc ccgcttgcg caacctgac 
      ctactcggc ccccttgta cgtcgatat caccaagac 
      catagtcaa ggacggcga ggaccccat tgagaccca 
      gcaccagaa aaccttcat cgggaaaat cccgattat 
      gctccgatc cacgtactg tctcctcaa cggtctaac 
      cgaccgcga cttgaccga actcaacga gtgccccct 
      tgaccccgg cggctactt catcatcaa cggctcaga 
      gaaagtcct aatcgccca ggagaaaat ggccacgaa 
      caccgtcta cgtcttctc gatgaaaga cgggaaata 
      cgccttcaa atccgaaat ccgctcctg tctcgagca 
      cagctcgcg ccccacgtc caccctctg ggtcaacat 
      gatggcacg tgggggcca ggcgatcaa aaaggccgc 
      catcgggca acgaatcat cgcgatctt gccctacat 
      caagcagga gatccccat catgatcgt gttccgggc 
      cttggggtt cgtggccga tcgggacat cctcgagca 
      cattattta cgacttcga agaccccga gatgatgga 
      aatggtgaa accgtccct ggacgaagc gttcgtgat 
      ccaggaaca gaacatcgc actcaactt cattgggac 
      caggggggc ccggccggg cgtcaccaa agaaaagcg 
      tatcaaata cgcgcgaga gatcctcca gaaggagat 
      gctgcccca cgttggagt gtccgattt ttgcgagac 
      gaaaaaagc ctacttttt ggggtacat ggtgcaccg 
      gttgctcct ggcggcttt ggggcgccg cgagctgga 
      cgatcggga tcactacgg taacaaacg actggactt 
      ggcggggcc cctgcttgc gtttttgtt ccgtgggtt 
      gtttaaaaa cttgatgaa ggaagtacg gatgtatgc 
      ccagaaatt tattgatcg gggcaaaga ctttaacct 
      cgacttggc cattaagac taagttgat tacggacgg 
      gttgaggta ttctctggc gactggtaa ctgggggga 
      ccagaagaa ggcccacca ggccagagc cggtgtctc 
      gcaggtgtt gaaccgttt gacttttgc ctcgacttt 
      gtcgcactt gagacgtgt taattcgcc cattggacg 
      tgacgggaa gttggcgaa gccccgaca gttgcacaa 
      cacgctctg ggggatgat ttgtccggc ggagacacc 
      agaaggggc ggctgttgg tctggtgaa aaatctggc 
      cctcatggc gtatatttc ggtggggtc gcaaccctc 
      tccaatttt ggagttttt ggaggagtg gtcgatgga 
      gaatttgga ggagattgc gccgtctgc catagccaa 
      cgcgaccaa gatttttgt taatggttg ctgggtggg 
      aattcacag ggaccccga acagttgat ggcgacgtt 
      acgcaagtt gaggagaca gatggatat tattgtgtc 
      ggaggtttc catgattcg ggatattcg ggaccggga 
      gattcgcat ttacacgga tgctggacg gatttgtcg 
      gccgttact aatcgtgga aaatgggac tttattgtt 
      gaagaagcg ccacattga tatgttgaa agagaggga 
      atataataa ctacgggtg gcaggtttt ggtggcttc 
      aggggttgt cgaatatat cgatacttt ggaagagga 
      gactgtgat gattgcgat gtctccgga cgatttgag 
      acaggagaa ggaatacgc gtattgtac cacttacac 
      acattgtga gatccatcc agctatgat tttgggtgt 
      ttgtgccag tattatacc cttcccgga tcacaacca 
      gagtcccag aaacacata tcaaagtgc tatgggcaa 
      gcaagccat gggtgtgta catcaccaa cttccatgt 
      ccgcatgga cacccttgc ccacgtcct gtattaccc 
      ccacaaacc gttggttac aaccagatc aatggaata 
      tctcagatt cagggaatt accggctgg gattaacag 
      catcgtggc cattgcgtg ttacaccgg ttataacca 
      ggaggattc ggttatttt gaacgcgtc ggccgtcga 
      aaggggatt tttccgatc tgtgtttta ccgctcgta 
      caaggacgc cgaatcgaa acgcatcgg cgaccaaga 
      ggagcagtt cgagaaacc gactcgcca gacctgcca 
      aggcatgcg caacgccct ctacgacaa actggacga 
      tgacggcat catagcacc aggaatccg tgtctctgg 
      cgacgacgt cgtcatcgg caaaacaat gacactgcc 
      ggaaaacga cgacgagtt ggacggcac caccaaacg 
      tttcacaaa acgtgacgc ctccacgtt cctccggaa 
      cagcgaaac gggggtggt ggaccaggt catgctcac 
      cctcaactc ggagggcta caaattctg caaaattag 
      ggtgcgttc cgtgcgcat cccccaaat tggggacaa 
      gttcgcgtc acggcacgg acagaaggg cacctgcgg 
      gatacagta ccgccagga ggacatgat tttcacgtg 
      cgagggcct aacccctga cattataat caaccccca 
      tgccatccc ctcgcgtat gaccatcgg gcacttgat 
      tgagtgcat ccaaggcaa agtctcggc aaacaaggg 
      cgaaatcgg cgacgctac cccgtttaa tgacgccgt 
      caacgtgca gaaaatctc aactttgct acaagagta 
      cgggtatca gctccgagg caacgaagt gatgtacaa 
      tggacacac aggccggaa aatcaacgc gcagatttt 
      cttaggccc gacgtatta tcagagact gaagcacat 
      ggtcgacga taagataca ttcgagggc gcgagggcc 
      cgtccagat tttggtgcg acaacccat ggaagggag 
      ggcccgcga tggggggct gcggtttgg ggaaatgga 
      acgtgattg tcagatttc gcacggagc ggcacagtt 
      tctacgcga gaggttgtt cgaggtttc ggatcagta 
      tagaataca tgtttgtaa tttttgcgg gttgattgc 
      catcgctaa tatgaggaa taacacgtt cgagtgcaa 
      ggggtgcaa aaataagac gcagatttc gcaagtgag 
      gctgccgta tgcggccaa gttgctctt ccaggagct 
      gatgtcgat gaatatcgc gccgcggtt gatggtttt 
      gtaattaga ggtaggtac gaataaagt attttttct 
      gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY