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Gene information
General information
TC011763 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13815608..13832800
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011763
    iBeetle screen iB_05098

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna circumference increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    Pupal morphology
    labrum orientation irregular - Number of animals showing the phenotype: 5

    antenna orientation irregular - Number of animals showing the phenotype: 5

    legs partially not present - Number of animals showing the phenotype: 5
    legs partially size shortened - Number of animals showing the phenotype: 5

    wings not present - Number of animals showing the phenotype: 5

    abdomen gin-traps size shortened - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011763 RA
      MMPLAPTPI VAVPSKPKI GFSIDSIVG QSPQHSPVH 
      FSEGSAPLS PSSDDNQPR SPASPKPPI MVPGIPAHS 
      LVRPSPTYD PNLVPKNGF LQPEMVHNH QQHPFALAA 
      HFQGAALAA ALSSGQHGF PQPSPLATH QPRDSYPLY 
      PWLLSRHGR IFPHRFPGG PDIPGFLLQ PFRKPKRIR 
      TAFSPSQLL KLEHAFEKN HYVVGAERK QLAQSLSLT 
      ETQVKVWFQ NRRTKHKRM QQEEEAKTS QGGNKSGSQ 
      GGQQNSHHV NKWKQETGD DGQYGEYID MDMDEECVS 
      DVESET
    CDS sequence
    • >TC011763 RA
      atgatgccg ttggcaccg acccccatc gtggcggtg 
      ccctccaag cccaagatc ggcttctcc atcgactcc 
      atcgtgggg cagagcccc cagcactcg ccggtgcac 
      ttctccgag ggctcggcg ccgctctcg ccctccagc 
      gatgacaac cagccgcgg tcgcccgcc tcccccaag 
      ccgcccatc atggtgccg ggcatcccg gcgcactcg 
      ctggtgagg cccagcccc acctacgac cccaacctg 
      gtgcccaag aacggcttc ctccagccg gagatggtg 
      cacaaccac cagcagcat cccttcgcc ttggcggcg 
      cacttccag ggagcggcg ctggcggcg gcgctcagc 
      tccggacag cacggcttc ccgcagccg tcgccgctc 
      gccacccac cagccccgg gacagctac cccctgtat 
      ccctggctg ctctccagg cacggcagg atcttcccc 
      cacaggttc ccgggagga ccggacatt cccggcttc 
      ctcctgcag cccttcagg aaaccaaag agaatacgg 
      acggcgttt agtccgtcg caactgctc aagctcgag 
      cacgctttc gagaaaaat cactatgtc gtaggtgcg 
      gaacgaaag cagcttgca cagtcgtta tcgctgact 
      gaaactcag gtgaaggtg tggttccag aaccggcgg 
      acgaagcac aagcggatg cagcaggag gaagaggcg 
      aagacgagc cagggcggg aacaagtcg ggctcgcag 
      ggcggccag cagaacagc caccacgtc aacaagtgg 
      aagcaggag acgggggat gacggccaa tacggggag 
      tacatcgac atggacatg gacgaggag tgtgtgagt 
      gatgtagag agtgaaaca taa
    mRNA sequence
    • >TC011763 RA
      tgaagagtt tcggtgacg gtagcacgt actcgtgcg 
      ctaactaag aataattgc tccaattgt cgtaatggt 
      cctgtagtg tttagtgtt gggtgcgat gtggtgtgc 
      gtgaccatg gcctgctaa gactgtcat gatgatgcc 
      gttggcacc gacccccat cgtggcggt gccctccaa 
      gcccaagat cggcttctc catcgactc catcgtggg 
      gcagagccc ccagcactc gccggtgca cttctccga 
      gggctcggc gccgctctc gccctccag cgatgacaa 
      ccagccgcg gtcgcccgc ctcccccaa gccgcccat 
      catggtgcc gggcatccc ggcgcactc gctggtgag 
      gcccagccc cacctacga ccccaacct ggtgcccaa 
      gaacggctt cctccagcc ggagatggt gcacaacca 
      ccagcagca tcccttcgc cttggcggc gcacttcca 
      gggagcggc gctggcggc ggcgctcag ctccggaca 
      gcacggctt cccgcagcc gtcgccgct cgccaccca 
      ccagccccg ggacagcta ccccctgta tccctggct 
      gctctccag gcacggcag gatcttccc ccacaggtt 
      cccgggagg accggacat tcccggctt cctcctgca 
      gcccttcag gaaaccaaa gagaatacg gacggcgtt 
      tagtccgtc gcaactgct caagctcga gcacgcttt 
      cgagaaaaa tcactatgt cgtaggtgc ggaacgaaa 
      gcagcttgc acagtcgtt atcgctgac tgaaactca 
      ggtgaaggt gtggttcca gaaccggcg gacgaagca 
      caagcggat gcagcagga ggaagaggc gaagacgag 
      ccagggcgg gaacaagtc gggctcgca gggcggcca 
      gcagaacag ccaccacgt caacaagtg gaagcagga 
      gacggggga tgacggcca atacgggga gtacatcga 
      catggacat ggacgagga gtgtgtgag tgatgtaga 
      gagtgaaac ataaactgt gtacatagt cgtgcgaga 
      cgatagtgc caactttta cgtgccaaa ctgttcgtt 
      agtgccaag tccccagta gagtagtcc cagtgtaaa 
      tacggccct ttatttatt gcaaggcat ccctataca 
      agttttgta tttattttc tgtacatag gcattgtga 
      atactttat cgagaagac accgtttgt tttcctgtg 
      attatcctg gtttatttt ccacatatc taacgattt 
      tctacataa atatgtgtg tataataga cgtttgttg 
      aaggagatt ttttacttg tacattctt ttaaggcga 
      gacttttga tttttaata aactcctat taaaaattg 
      aacctctca tttactaga cttaactgt agaaagcta 
      tgttgagta tgatgtact taaaacaac tgtaaagaa 
      cacgaaata tacctaaaa gaaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY