Status
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Gene information
General information
TC034444
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011754,TC011755
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13912026..13939224
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034444
    iBeetle screen iB_05097

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034444 RA
      MLITEGSAM GERVEPVVP GPDLVGVNV ERPPAPLVH 
      QQSTVWSKR QHAVCVRHA FKQYGSKKN PNHVLSDLN 
      MTVAKGTIY GLLGASGCG KTTLLSCIV GRRRLNTGE 
      IWVLGGKPG TKGSGVPGK RVGYMPQEI ALYGEFSIK 
      ETMMYFGWI FGMESSEIN ERLQFLLNF LDLPSQNRL 
      VKNLSGGQQ RRVSFAVAL MHDPELLIL DEPTVGVDP 
      LLRQSIWNH LVQITKDGN KTVIITTHY IEEARQAHT 
      IGLMRSGKL LAEESPHVL LSMYGCQSL EEVFLKLSR 
      KQGQANQND INISNNISL ATLNWGKKD SISVTEESG 
      VVGLNFHQS KEILIADST NGHIDQLGK PSSSKSSMA 
      DACDDCSCS DMTSWGKIK ALLQKNFLR MWRNVGVML 
      FIFALPVMQ VILFCLAIG RDPTGLKLA IVNHEKNYT 
      NQSYQECSF DYGCKFSYL SCRYLNNLR NSTILKEYY 
      PDPESAVDA VKQGHAWGA LYFTENFTD ALVARMALG 
      KDADPETLD QSEVRVWLD MSNQQIGII LQRDLQLSY 
      QDFAKDLLG ACEQNPDLA EIPISFKEP IYGSNKPSF 
      TDFVAPGVI LTIVFFLAV ALTSSALII ERMEGLLDR 
      SWVAGVTPG EILFSHVVT QFVVMCGQT ALVLIFMIL 
      VFGVQCKGD IGWVIVLTI LQGLCGMCF GFVISAICE 
      LERNAIQLA LGSFYPTLL LSGVIWPIE GMPTVLRYV 
      STFLPLTLA TTSLRAMLT RGWSIAEPA VYYGFLATI 
      IWIVAFLTI SMLVLRFKR G
    • >TC034444 RB
      MGEDDKLKR MFSWSSNAL SEGSAMGER VEPVVPGPD 
      LVGVNVERP PAPLVHQQS TVWSKRQHA VCVRHAFKQ 
      YGSKKNPNH VLSDLNMTV AKGTIYGLL GASGCGKTT 
      LLSCIVGRR RLNTGEIWV LGGKPGTKG SGVPGKRVG 
      YMPQEIALY GEFSIKETM MYFGWIFGM ESSEINERL 
      QFLLNFLDL PSQNRLVKN LSGGQQRRV SFAVALMHD 
      PELLILDEP TVGVDPLLR QSIWNHLVQ ITKDGNKTV 
      IITTHYIEE ARQAHTIGL MRSGKLLAE ESPHVLLSM 
      YGCQSLEEV FLKLSRKQG QANQNDINI SNNISLATL 
      NWGKKDSIS VTEESGVVG LNFHQSKEI LIADSTNGH 
      IDQLGKPSS SKSSMADAC DDCSCSDMT SWGKIKALL 
      QKNFLRMWR NVGVMLFIF ALPVMQVIL FCLAIGRDP 
      TGLKLAIVN HEKNYTNQS YQECSFDYG CKFSYLSCR 
      YLNNLRNST ILKEYYPDP ESAVDAVKQ GHAWGALYF 
      TENFTDALV ARMALGKDA DPETLDQSE VRVWLDMSN 
      QQIGIILQR DLQLSYQDF AKDLLGACE QNPDLAEIP 
      ISFKEPIYG SNKPSFTDF VAPGVILTI VFFLAVALT 
      SSALIIERM EGLLDRSWV AGVTPGEIL FSHVVTQFV 
      VMCGQTALV LIFMILVFG VQCKGDIGW VIVLTILQG 
      LCGMCFGFV ISAICELER NAIQLALGS FYPTLLLSG 
      VIWPIEGMP TVLRYVSTF LPLTLATTS LRAMLTRGW 
      SIAEPAVYY GFLATIIWI VAFLTISML VLRFKRG
    • >TC034444 RC
      MGEDDKLKR MFSWSSNAL RKSPSEQRE RVTQPPIIS 
      ETSQSESPH RWRGAGVVY RKTSMVSPA TNLSRNRRV 
      CSSGLFRAI ECTSGEPPQ CNHTIPSVR KMC
    CDS sequence
    • >TC034444 RA
      atgttgata actgaaggt tcagcgatg ggcgagcgc 
      gtcgagccc gtggtgcca ggcccggac ttggtcggc 
      gtgaacgtg gaaaggccg ccggcgccc ctggtccac 
      cagcagtcc accgtctgg agcaaaaga cagcacgcc 
      gtctgcgtg cgacacgct ttcaagcag tacggctcc 
      aagaagaac cccaaccac gtcctcagc gacctcaac 
      atgaccgtc gccaaaggc acaatctac gggttgttg 
      ggggcgtct ggctgcggc aaaaccacc ctcttgtcg 
      tgcatcgtg ggccgtcgg cggctgaac acgggggag 
      atctgggtg ttggggggc aaaccggga accaagggc 
      tcgggcgtg cctggaaaa agggtgggc tacatgccc 
      caggagatc gccctgtac ggcgaattc tccatcaag 
      gaaaccatg atgtacttc ggctggatc ttcgggatg 
      gagtcctcg gaaatcaac gaacgctta caattcctg 
      ttgaacttc ctggacctc ccgagccaa aaccgccta 
      gtgaagaac ctaagtggc ggccaacag cgccgtgtc 
      tccttcgcg gtcgcccta atgcacgac cccgagctc 
      ttgatcctt gacgagccc accgtcggg gtggatccg 
      ctcctgagg cagagtatc tggaaccac ttggtccag 
      attacgaag gatggcaac aaaacggtt atcattacg 
      acgcattat atcgaagag gcgcggcaa gctcatacc 
      atcgggttg atgcgaagc gggaaattg ctggcggag 
      gagtcgccg catgttttg ctctctatg tacggctgt 
      cagtcgtta gaggaagtg tttttgaaa ctgtccagg 
      aagcaggga caagccaac caaaatgat atcaacatt 
      agcaataat attagtcta gcgacgctc aactggggc 
      aagaaggac tcgatttcg gtgacggag gagagcgga 
      gtcgtcgga ttgaacttc caccaaagc aaagaaata 
      cttatagcg gatagcacc aacggacac atcgatcag 
      ctgggcaag ccgtcgtcg tcgaagagc agcatggcg 
      gacgcctgc gacgactgc agctgctcc gacatgacc 
      tcctggggc aagatcaag gccctgctc cagaagaac 
      ttcctccgc atgtggcgc aacgtcggc gtgatgctg 
      ttcatcttc gcactgccc gtcatgcaa gtgatactg 
      ttttgccta gccatcggg cgcgacccc accggcctg 
      aagctggcc atcgtcaac cacgagaag aactacacc 
      aaccagtcc taccaggag tgctcgttc gactacggc 
      tgcaagttc tcctatcta agttgccgc tacttgaac 
      aatctgcgg aactcgacc atcctcaaa gagtactat 
      ccggacccg gagtcggcg gtcgacgcc gtcaagcag 
      ggccacgcc tggggcgcc ctctacttc accgagaac 
      ttcacggac gcgctggtg gctcgcatg gcgctgggc 
      aaagacgcc gatccggag acgttggac cagagcgaa 
      gtccgggtg tggctggac atgtcgaac cagcaaatc 
      ggaattata ctgcaacga gacttgcaa ttgtcctat 
      caagacttt gcgaaagac ttgctagga gcgtgcgag 
      caaaatccc gatttggcc gaaattccc atctccttc 
      aaggagccc atttatggg tcgaataaa ccgtcgttt 
      accgatttt gtggccccg ggggttatc ctaacgatt 
      gttttcttc ctcgctgtc gcgctcaca tcctcggct 
      ttgatcatt gaacggatg gaagggctg ctggaccgg 
      tcttgggtt gctggtgta acccccggc gagatcctc 
      ttcagccac gtggtgacc cagttcgtg gtgatgtgc 
      gggcagacg gccctcgtg ctcatcttc atgatcctg 
      gtgttcggc gtgcagtgc aagggcgac atcggctgg 
      gtgatcgtc ctcaccatc ctgcagggc ctgtgcggc 
      atgtgcttc ggcttcgtg atctcggcg atctgcgag 
      ctggagcgc aacgccatc cagctggcc ctcggcagc 
      ttctacccc acgctgctg ctcagcggc gtgatctgg 
      cccatcgag ggcatgccc acggtgctg cgctacgtc 
      tccaccttc ctgccgctg acgctggcc accaccagc 
      ctgcgggcg atgctcacc cggggctgg tccatcgcc 
      gagccggcc gtctactac ggcttcctg gcgaccatc 
      atctggatc gtggccttc ctgacgatc agcatgctg 
      gtacttagg ttcaagaga gggtag
    • >TC034444 RB
      atgggtgaa gacgacaag ctgaagagg atgttcagc 
      tggagcagc aacgccttg agtgaaggt tcagcgatg 
      ggcgagcgc gtcgagccc gtggtgcca ggcccggac 
      ttggtcggc gtgaacgtg gaaaggccg ccggcgccc 
      ctggtccac cagcagtcc accgtctgg agcaaaaga 
      cagcacgcc gtctgcgtg cgacacgct ttcaagcag 
      tacggctcc aagaagaac cccaaccac gtcctcagc 
      gacctcaac atgaccgtc gccaaaggc acaatctac 
      gggttgttg ggggcgtct ggctgcggc aaaaccacc 
      ctcttgtcg tgcatcgtg ggccgtcgg cggctgaac 
      acgggggag atctgggtg ttggggggc aaaccggga 
      accaagggc tcgggcgtg cctggaaaa agggtgggc 
      tacatgccc caggagatc gccctgtac ggcgaattc 
      tccatcaag gaaaccatg atgtacttc ggctggatc 
      ttcgggatg gagtcctcg gaaatcaac gaacgctta 
      caattcctg ttgaacttc ctggacctc ccgagccaa 
      aaccgccta gtgaagaac ctaagtggc ggccaacag 
      cgccgtgtc tccttcgcg gtcgcccta atgcacgac 
      cccgagctc ttgatcctt gacgagccc accgtcggg 
      gtggatccg ctcctgagg cagagtatc tggaaccac 
      ttggtccag attacgaag gatggcaac aaaacggtt 
      atcattacg acgcattat atcgaagag gcgcggcaa 
      gctcatacc atcgggttg atgcgaagc gggaaattg 
      ctggcggag gagtcgccg catgttttg ctctctatg 
      tacggctgt cagtcgtta gaggaagtg tttttgaaa 
      ctgtccagg aagcaggga caagccaac caaaatgat 
      atcaacatt agcaataat attagtcta gcgacgctc 
      aactggggc aagaaggac tcgatttcg gtgacggag 
      gagagcgga gtcgtcgga ttgaacttc caccaaagc 
      aaagaaata cttatagcg gatagcacc aacggacac 
      atcgatcag ctgggcaag ccgtcgtcg tcgaagagc 
      agcatggcg gacgcctgc gacgactgc agctgctcc 
      gacatgacc tcctggggc aagatcaag gccctgctc 
      cagaagaac ttcctccgc atgtggcgc aacgtcggc 
      gtgatgctg ttcatcttc gcactgccc gtcatgcaa 
      gtgatactg ttttgccta gccatcggg cgcgacccc 
      accggcctg aagctggcc atcgtcaac cacgagaag 
      aactacacc aaccagtcc taccaggag tgctcgttc 
      gactacggc tgcaagttc tcctatcta agttgccgc 
      tacttgaac aatctgcgg aactcgacc atcctcaaa 
      gagtactat ccggacccg gagtcggcg gtcgacgcc 
      gtcaagcag ggccacgcc tggggcgcc ctctacttc 
      accgagaac ttcacggac gcgctggtg gctcgcatg 
      gcgctgggc aaagacgcc gatccggag acgttggac 
      cagagcgaa gtccgggtg tggctggac atgtcgaac 
      cagcaaatc ggaattata ctgcaacga gacttgcaa 
      ttgtcctat caagacttt gcgaaagac ttgctagga 
      gcgtgcgag caaaatccc gatttggcc gaaattccc 
      atctccttc aaggagccc atttatggg tcgaataaa 
      ccgtcgttt accgatttt gtggccccg ggggttatc 
      ctaacgatt gttttcttc ctcgctgtc gcgctcaca 
      tcctcggct ttgatcatt gaacggatg gaagggctg 
      ctggaccgg tcttgggtt gctggtgta acccccggc 
      gagatcctc ttcagccac gtggtgacc cagttcgtg 
      gtgatgtgc gggcagacg gccctcgtg ctcatcttc 
      atgatcctg gtgttcggc gtgcagtgc aagggcgac 
      atcggctgg gtgatcgtc ctcaccatc ctgcagggc 
      ctgtgcggc atgtgcttc ggcttcgtg atctcggcg 
      atctgcgag ctggagcgc aacgccatc cagctggcc 
      ctcggcagc ttctacccc acgctgctg ctcagcggc 
      gtgatctgg cccatcgag ggcatgccc acggtgctg 
      cgctacgtc tccaccttc ctgccgctg acgctggcc 
      accaccagc ctgcgggcg atgctcacc cggggctgg 
      tccatcgcc gagccggcc gtctactac ggcttcctg 
      gcgaccatc atctggatc gtggccttc ctgacgatc 
      agcatgctg gtacttagg ttcaagaga gggtag
    • >TC034444 RC
      atgggtgaa gacgacaag ctgaagagg atgttcagc 
      tggagcagc aacgccttg agaaaaagc ccatccgag 
      caacgggaa cgcgtgacg caaccgccc attatcagt 
      gaaacctcc caaagcgag tccccccat cgctggaga 
      ggggcagga gttgtttat cgcaagact tcgatggtc 
      agtccggcg actaacctc tcccgcaat cgacgcgtg 
      tgctcctcc ggacttttc cgggcaata gaatgtacg 
      agtggcgaa ccaccgcag tgtaatcat accataccg 
      agtgttcga aaaatgtgc tag
    mRNA sequence
    • >TC034444 RA
      aggggcagg agttgttta tcgcaagac ttcgatggt 
      cagtccggc gactaacct ctcccgcaa tcgacgcgt 
      gtgctcctc cggactttt ccgggcaat agaatgtac 
      gagtggcga accaccgca gtgtaatca taccatacc 
      gagtgttcg aaaaatgtg ctagtgaaa gttgcgcca 
      gccgaaatg ttgataact gaaggttca gcgatgggc 
      gagcgcgtc gagcccgtg gtgccaggc ccggacttg 
      gtcggcgtg aacgtggaa aggccgccg gcgcccctg 
      gtccaccag cagtccacc gtctggagc aaaagacag 
      cacgccgtc tgcgtgcga cacgctttc aagcagtac 
      ggctccaag aagaacccc aaccacgtc ctcagcgac 
      ctcaacatg accgtcgcc aaaggcaca atctacggg 
      ttgttgggg gcgtctggc tgcggcaaa accaccctc 
      ttgtcgtgc atcgtgggc cgtcggcgg ctgaacacg 
      ggggagatc tgggtgttg gggggcaaa ccgggaacc 
      aagggctcg ggcgtgcct ggaaaaagg gtgggctac 
      atgccccag gagatcgcc ctgtacggc gaattctcc 
      atcaaggaa accatgatg tacttcggc tggatcttc 
      gggatggag tcctcggaa atcaacgaa cgcttacaa 
      ttcctgttg aacttcctg gacctcccg agccaaaac 
      cgcctagtg aagaaccta agtggcggc caacagcgc 
      cgtgtctcc ttcgcggtc gccctaatg cacgacccc 
      gagctcttg atccttgac gagcccacc gtcggggtg 
      gatccgctc ctgaggcag agtatctgg aaccacttg 
      gtccagatt acgaaggat ggcaacaaa acggttatc 
      attacgacg cattatatc gaagaggcg cggcaagct 
      cataccatc gggttgatg cgaagcggg aaattgctg 
      gcggaggag tcgccgcat gttttgctc tctatgtac 
      ggctgtcag tcgttagag gaagtgttt ttgaaactg 
      tccaggaag cagggacaa gccaaccaa aatgatatc 
      aacattagc aataatatt agtctagcg acgctcaac 
      tggggcaag aaggactcg atttcggtg acggaggag 
      agcggagtc gtcggattg aacttccac caaagcaaa 
      gaaatactt atagcggat agcaccaac ggacacatc 
      gatcagctg ggcaagccg tcgtcgtcg aagagcagc 
      atggcggac gcctgcgac gactgcagc tgctccgac 
      atgacctcc tggggcaag atcaaggcc ctgctccag 
      aagaacttc ctccgcatg tggcgcaac gtcggcgtg 
      atgctgttc atcttcgca ctgcccgtc atgcaagtg 
      atactgttt tgcctagcc atcgggcgc gaccccacc 
      ggcctgaag ctggccatc gtcaaccac gagaagaac 
      tacaccaac cagtcctac caggagtgc tcgttcgac 
      tacggctgc aagttctcc tatctaagt tgccgctac 
      ttgaacaat ctgcggaac tcgaccatc ctcaaagag 
      tactatccg gacccggag tcggcggtc gacgccgtc 
      aagcagggc cacgcctgg ggcgccctc tacttcacc 
      gagaacttc acggacgcg ctggtggct cgcatggcg 
      ctgggcaaa gacgccgat ccggagacg ttggaccag 
      agcgaagtc cgggtgtgg ctggacatg tcgaaccag 
      caaatcgga attatactg caacgagac ttgcaattg 
      tcctatcaa gactttgcg aaagacttg ctaggagcg 
      tgcgagcaa aatcccgat ttggccgaa attcccatc 
      tccttcaag gagcccatt tatgggtcg aataaaccg 
      tcgtttacc gattttgtg gccccgggg gttatccta 
      acgattgtt ttcttcctc gctgtcgcg ctcacatcc 
      tcggctttg atcattgaa cggatggaa gggctgctg 
      gaccggtct tgggttgct ggtgtaacc cccggcgag 
      atcctcttc agccacgtg gtgacccag ttcgtggtg 
      atgtgcggg cagacggcc ctcgtgctc atcttcatg 
      atcctggtg ttcggcgtg cagtgcaag ggcgacatc 
      ggctgggtg atcgtcctc accatcctg cagggcctg 
      tgcggcatg tgcttcggc ttcgtgatc tcggcgatc 
      tgcgagctg gagcgcaac gccatccag ctggccctc 
      ggcagcttc taccccacg ctgctgctc agcggcgtg 
      atctggccc atcgagggc atgcccacg gtgctgcgc 
      tacgtctcc accttcctg ccgctgacg ctggccacc 
      accagcctg cgggcgatg ctcacccgg ggctggtcc 
      atcgccgag ccggccgtc tactacggc ttcctggcg 
      accatcatc tggatcgtg gccttcctg acgatcagc 
      atgctggta cttaggttc aagagaggg tagtgccgc 
      tggatacgt actgtacaa agtcgcaaa attgttgtg 
      gttatagtc attttttat ttataaata acttgcctt 
      gatgtttaa cataagtgt tacacctag tttaagttt 
      tatatacag ttttgtatt atgacgcat gtcgccggt 
      cacagctga tggctttaa cacaagacg gacgccttt 
      ggcagtaga gacgtttct agaagtttt aatacaaag 
      tgctcaatt ttatattgt taatggtat tgtgatgta 
      tgtttgttt agtgatatt aataaaaat atatgcaga 
      ac
    • >TC034444 RB
      tacagcaga aatcaatta acgcaacga caaaaattg 
      atcgacagc caacgtaat aatttcatc catactaca 
      attactttt ctgaaccaa attaaatga agtaattat 
      tttaatcca accctaaat cgacgttta ttatctcgt 
      ccaaagatt gcaattgtg ccacgtgac tgtccgggc 
      gccgcgatt ggcttgcgt acggcgcca actgcccca 
      tttgaaatt tccgtgaat ctaattggc cctaaggtg 
      tcacgtgtg cgaataccg tttcgtcat tggtttaca 
      taacaatct gagatgttt ccttcgaaa ccaaaagtg 
      tttccctct ctccgcggc caactttca tttatgtcc 
      tgtgcagtt cgttgcacg caacttgtt cttagcacc 
      aataaaacg ctagaaagg catcttgtt cactttcta 
      tcgcatcag tgtcgaaag cggcagttg ttttccaga 
      gttgaagaa aatcggtag agggtgcgt caacccccg 
      cgtctcata aaattttca caaggacag caggtgtct 
      cgattccgt cagttagtt ggacgatgt ggtgaagtc 
      ggcgcgtgt tttccgatt ttccgcaat gggtgaaga 
      cgacaagct gaagaggat gttcagctg gagcagcaa 
      cgccttgag tgaaggttc agcgatggg cgagcgcgt 
      cgagcccgt ggtgccagg cccggactt ggtcggcgt 
      gaacgtgga aaggccgcc ggcgcccct ggtccacca 
      gcagtccac cgtctggag caaaagaca gcacgccgt 
      ctgcgtgcg acacgcttt caagcagta cggctccaa 
      gaagaaccc caaccacgt cctcagcga cctcaacat 
      gaccgtcgc caaaggcac aatctacgg gttgttggg 
      ggcgtctgg ctgcggcaa aaccaccct cttgtcgtg 
      catcgtggg ccgtcggcg gctgaacac gggggagat 
      ctgggtgtt ggggggcaa accgggaac caagggctc 
      gggcgtgcc tggaaaaag ggtgggcta catgcccca 
      ggagatcgc cctgtacgg cgaattctc catcaagga 
      aaccatgat gtacttcgg ctggatctt cgggatgga 
      gtcctcgga aatcaacga acgcttaca attcctgtt 
      gaacttcct ggacctccc gagccaaaa ccgcctagt 
      gaagaacct aagtggcgg ccaacagcg ccgtgtctc 
      cttcgcggt cgccctaat gcacgaccc cgagctctt 
      gatccttga cgagcccac cgtcggggt ggatccgct 
      cctgaggca gagtatctg gaaccactt ggtccagat 
      tacgaagga tggcaacaa aacggttat cattacgac 
      gcattatat cgaagaggc gcggcaagc tcataccat 
      cgggttgat gcgaagcgg gaaattgct ggcggagga 
      gtcgccgca tgttttgct ctctatgta cggctgtca 
      gtcgttaga ggaagtgtt tttgaaact gtccaggaa 
      gcagggaca agccaacca aaatgatat caacattag 
      caataatat tagtctagc gacgctcaa ctggggcaa 
      gaaggactc gatttcggt gacggagga gagcggagt 
      cgtcggatt gaacttcca ccaaagcaa agaaatact 
      tatagcgga tagcaccaa cggacacat cgatcagct 
      gggcaagcc gtcgtcgtc gaagagcag catggcgga 
      cgcctgcga cgactgcag ctgctccga catgacctc 
      ctggggcaa gatcaaggc cctgctcca gaagaactt 
      cctccgcat gtggcgcaa cgtcggcgt gatgctgtt 
      catcttcgc actgcccgt catgcaagt gatactgtt 
      ttgcctagc catcgggcg cgaccccac cggcctgaa 
      gctggccat cgtcaacca cgagaagaa ctacaccaa 
      ccagtccta ccaggagtg ctcgttcga ctacggctg 
      caagttctc ctatctaag ttgccgcta cttgaacaa 
      tctgcggaa ctcgaccat cctcaaaga gtactatcc 
      ggacccgga gtcggcggt cgacgccgt caagcaggg 
      ccacgcctg gggcgccct ctacttcac cgagaactt 
      cacggacgc gctggtggc tcgcatggc gctgggcaa 
      agacgccga tccggagac gttggacca gagcgaagt 
      ccgggtgtg gctggacat gtcgaacca gcaaatcgg 
      aattatact gcaacgaga cttgcaatt gtcctatca 
      agactttgc gaaagactt gctaggagc gtgcgagca 
      aaatcccga tttggccga aattcccat ctccttcaa 
      ggagcccat ttatgggtc gaataaacc gtcgtttac 
      cgattttgt ggccccggg ggttatcct aacgattgt 
      tttcttcct cgctgtcgc gctcacatc ctcggcttt 
      gatcattga acggatgga agggctgct ggaccggtc 
      ttgggttgc tggtgtaac ccccggcga gatcctctt 
      cagccacgt ggtgaccca gttcgtggt gatgtgcgg 
      gcagacggc cctcgtgct catcttcat gatcctggt 
      gttcggcgt gcagtgcaa gggcgacat cggctgggt 
      gatcgtcct caccatcct gcagggcct gtgcggcat 
      gtgcttcgg cttcgtgat ctcggcgat ctgcgagct 
      ggagcgcaa cgccatcca gctggccct cggcagctt 
      ctaccccac gctgctgct cagcggcgt gatctggcc 
      catcgaggg catgcccac ggtgctgcg ctacgtctc 
      caccttcct gccgctgac gctggccac caccagcct 
      gcgggcgat gctcacccg gggctggtc catcgccga 
      gccggccgt ctactacgg cttcctggc gaccatcat 
      ctggatcgt ggccttcct gacgatcag catgctggt 
      acttaggtt caagagagg gtagtgccg ctggatacg 
      tactgtaca aagtcgcaa aattgttgt ggttatagt 
      catttttta tttataaat aacttgcct tgatgttta 
      acataagtg ttacaccta gtttaagtt ttatataca 
      gttttgtat tatgacgca tgtcgccgg tcacagctg 
      atggcttta acacaagac ggacgcctt tggcagtag 
      agacgtttc tagaagttt taatacaaa gtgctcaat 
      tttatattg ttaatggta ttgtgatgt atgtttgtt 
      tagtgatat taataaaaa tatatgcag aac
    • >TC034444 RC
      caacttgtg tttaaattt tcatagaaa acgaaaacc 
      ggacgttaa tagagttaa taatttaat ttgcagttg 
      aagaaaatc ggtagaggg tgcgtcaac ccccgcgtc 
      tcataaaat tttcacaag gacagcagg tgtctcgat 
      tccgtcagt tagttggac gatgtggtg aagtcggcg 
      cgtgttttc cgattttcc gcaatgggt gaagacgac 
      aagctgaag aggatgttc agctggagc agcaacgcc 
      ttgagaaaa agcccatcc gagcaacgg gaacgcgtg 
      acgcaaccg cccattatc agtgaaacc tcccaaagc 
      gagtccccc catcgctgg agaggggca ggagttgtt 
      tatcgcaag acttcgatg gtcagtccg gcgactaac 
      ctctcccgc aatcgacgc gtgtgctcc tccggactt 
      ttccgggca atagaatgt acgagtggc gaaccaccg 
      cagtgtaat cataccata ccgagtgtt cgaaaaatg 
      tgctagtga aagttgcgc cagccgaaa tgttgataa 
      cgtaagaca ttgaatttt aatttctgg ctcaaaacc 
      gaagccttc gccactctc ttggtattc ccggcgaaa 
      tcttccatc cactgaatg actcactaa ttgcgcaaa 
      gcctttgtg cccgcgccc gcagcaagg ccgcccctc 
      actttcccg caattaggc cggaaccca gcgcgttac 
      ataaatcgg cgctattag cggccgctt tctaacgac 
      cgccgctag ggcccagcg ttttgcaac gcgcaaaca 
      tcgccgcgt ctcgcagaa tacaaaaca ttgagcaac
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