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Gene information
General information
TC011748 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14014280..14041343
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011748
    iBeetle screen iB_05096

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva anterior irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011748 RA
      MQDTECLSP SSPQAMSPC LSRSPSPAL DDDEISVGC 
      PSPREPPDP EDYFKPLKK LKMLEMPTG ELDERSDGG 
      VKSFSIVDI LNHKPKVQA RIVRPWDLD PDVEAQQRL 
      ETFHRHLKV QQLALLRPE FAAFNASYT SETGSDRSS 
      SVASDCCSP DIVPAQRQR HQGKPQGTP LDALFQMTS 
      KTFDSGAGE TSQDAQSHL NLFNGRQQP KKKRKSRTA 
      FTNHQIFEL EKRFLYQKY LSPADRDEI ASSLGLTNA 
      QVITWFQNR RAKLKRDME ELKKDVESA KLLTAHKSF 
      LENVQDLGI LKKKAVISE DDCPKNLVV TTEK
    CDS sequence
    • >TC011748 RA
      atgcaggac acggagtgt ctgtccccc agcagcccc 
      caggccatg tccccctgt ctgtcgcgc agccccagc 
      ccggcgctg gacgacgac gagatcagc gtcggctgc 
      cccagcccc cgggagccc cccgacccc gaggactac 
      ttcaagccg ctgaagaag ctcaaaatg ctggagatg 
      ccgacgggc gagctggac gagcgcagc gacggcggc 
      gtgaagagc ttctccatc gtggacatc ctcaaccac 
      aagcccaag gtgcaggcg aggattgtg cggccctgg 
      gacctggac ccggacgtg gaggcgcag cagagactc 
      gagactttt cacagacac ctcaaagtg caacaattg 
      gcccttctg aggcccgaa ttcgcggct ttcaatgcg 
      agctacacg tcggaaacc ggaagtgat cggtcgtcg 
      agtgtcgcg agcgattgt tgttcaccg gatattgtg 
      ccagcgcaa agacagaga caccaaggg aagccgcaa 
      gggacgcct ctggatgca ctgttccag atgacgtcc 
      aaaaccttc gattcgggg gcgggagag acgagccaa 
      gacgctcag agccacctc aacctgttc aacgggcgg 
      cagcagccg aagaaaaag cgaaaatcg cgaacggcc 
      ttcaccaac caccagatc ttcgagctg gagaaacgg 
      ttcctttac caaaaatac ctcagtccg gccgaccgg 
      gacgagatc gcctccagc ctcggcctg accaacgcc 
      caagtcatc acctggttc cagaaccgg agagccaag 
      ctcaagcgg gacatggag gagctgaag aaggacgtc 
      gagagcgcg aaactgctc acagcgcac aaaagcttc 
      ctcgagaac gtccaggac ctgggcatt ttgaagaag 
      aaggcggtg atcagtgag gacgactgt cccaaaaac 
      ttggtcgtg acgacggaa aagtga
    mRNA sequence
    • >TC011748 RA
      ggtggtctc tccccccct caagtcccg ccttcgccc 
      aatcgccgc ccggcgcgc gccctcatt ggccgctcg 
      ccttcgtcg agggcgtgc cccgttaaa gagcccagc 
      tcgccaaca agacgttca cttgttacc agatctgtt 
      aaagatacg aacctggaa cacgtgtcg gctaacgag 
      tgcgttcaa agtgacaat ggcggcaat cgagtgaaa 
      caatgcagg acacggagt gtctgtccc ccagcagcc 
      cccaggcca tgtccccct gtctgtcgc gcagcccca 
      gcccggcgc tggacgacg acgagatca gcgtcggct 
      gccccagcc cccgggagc cccccgacc ccgaggact 
      acttcaagc cgctgaaga agctcaaaa tgctggaga 
      tgccgacgg gcgagctgg acgagcgca gcgacggcg 
      gcgtgaaga gcttctcca tcgtggaca tcctcaacc 
      acaagccca aggtgcagg cgaggattg tgcggccct 
      gggacctgg acccggacg tggaggcgc agcagagac 
      tcgagactt ttcacagac acctcaaag tgcaacaat 
      tggcccttc tgaggcccg aattcgcgg ctttcaatg 
      cgagctaca cgtcggaaa ccggaagtg atcggtcgt 
      cgagtgtcg cgagcgatt gttgttcac cggatattg 
      tgccagcgc aaagacaga gacaccaag ggaagccgc 
      aagggacgc ctctggatg cactgttcc agatgacgt 
      ccaaaacct tcgattcgg gggcgggag agacgagcc 
      aagacgctc agagccacc tcaacctgt tcaacgggc 
      ggcagcagc cgaagaaaa agcgaaaat cgcgaacgg 
      ccttcacca accaccaga tcttcgagc tggagaaac 
      ggttccttt accaaaaat acctcagtc cggccgacc 
      gggacgaga tcgcctcca gcctcggcc tgaccaacg 
      cccaagtca tcacctggt tccagaacc ggagagcca 
      agctcaagc gggacatgg aggagctga agaaggacg 
      tcgagagcg cgaaactgc tcacagcgc acaaaagct 
      tcctcgaga acgtccagg acctgggca ttttgaaga 
      agaaggcgg tgatcagtg aggacgact gtcccaaaa 
      acttggtcg tgacgacgg aaaagtgag acttccgga 
      cggggtgta aatattgtg tacatagtt tttgtgttt 
      gtaaaagtt gtaaaaatt acgatgctc taacataat 
      gtaattaga taatttatt gaagttttt cgtctgttt 
      gacgggtat ttgcaatat tttaatata cttaaaatg 
      tgtactata tagagcttt agtacttaa aactgtcct 
      agcgtcaat gtggatgag aaataaaca tgtacgaag 
      tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY