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Gene information
General information
TC011735 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14231652..14232885
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011735
    iBeetle screen iB_05093

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011735 RA
      MLALLCALF FVYFALHRL TIARTKSAT CLVGKTAIV 
      TGANSGIGY QTALNLASR GCRVIMADV SDLTQSRNN 
      VIKFTSNSN IIAKKIDLG SLQSVREFA SDIAKSEPR 
      LDILINNAG ISSSKSNRT GDGLHPVMQ INYFGHFLL 
      THLLIDLLK KSAPSRIVF TSSFMSFFH NLTLTNLNY 
      DADNGFASA AKIYCNSKL AQMIASDIF AAKLKGTGV 
      TSNSINPGI VNTTLLAQF FTVQTQLKP LQLITDLYL 
      RFVAKDAWE GSQVIVHCA VSKKLEGVS GRYFWNCVP 
      VFKPLQAQD GEFCRKIWG ETERLVKLA PEERL
    CDS sequence
    • >TC011735 RA
      atgttggcg cttctttgt gcgcttttt ttcgtttat 
      ttcgcgcta caccgactg acaattgca aggacaaaa 
      agtgcgaca tgcctcgtg ggcaaaacc gcaattgtt 
      acaggggcg aactcaggc ataggctac cagactgct 
      ctcaacctg gcttcgagg ggctgcaga gtgattatg 
      gccgatgtt agcgacctg acacaatca cgaaataac 
      gtgatcaaa ttcacaagc aacagcaac attattgcg 
      aaaaaaatc gacttgggt tcacttcaa tcagtgcga 
      gaattcgcc tccgacatc gcaaaaagc gagccaaga 
      ttagatatt ttgataaac aacgccgga ataagctcc 
      tccaaatca aacagaacc ggagatggt ctacacccg 
      gttatgcaa atcaattat ttcggccac ttcttgttg 
      acgcattta ttgattgat ttactgaag aaatcggca 
      ccaagtcgt atagttttc accagctct ttcatgtcg 
      tttttccac aacttgacc ctcacaaac ctcaactat 
      gacgcagac aacgggttt gcctccgct gcgaaaatt 
      tactgcaac tcgaaactg gctcaaatg atcgcttcc 
      gacattttt gccgcgaaa ttgaaagga accggcgtt 
      acttcaaac tcgatcaac ccggggatt gtcaacacc 
      acgcttttg gcacaattt ttcactgtg caaacgcag 
      ctcaaacct ttacagctc atcacagat ttgtatttg 
      cgatttgtg gccaaagac gcttgggag ggttcgcaa 
      gtcattgtg cattgcgcg gtttcaaaa aaactggaa 
      ggcgtgagc gggcggtat ttctggaat tgtgtgccc 
      gtttttaag cctttgcag gctcaggat ggcgagttt 
      tgcaggaaa atttggggc gaaacggag agactcgta 
      aagttggcg cccgaagag aggctttga
    mRNA sequence
    • >TC011735 RA
      acacttgtg tgaaggttg agtgtctgt ttactgagc 
      cacaatgtt ggcgcttct ttgtgcgct ttttttcgt 
      ttatttcgc gctacaccg actgacaat tgcaaggac 
      aaaaagtgc gacatgcct cgtgggcaa aaccgcaat 
      tgttacagg ggcgaactc aggcatagg ctaccagac 
      tgctctcaa cctggcttc gaggggctg cagagtgat 
      tatggccga tgttagcga cctgacaca atcacgaaa 
      taacgtgat caaattcac aagcaacag caacattat 
      tgcgaaaaa aatcgactt gggttcact tcaatcagt 
      gcgagaatt cgcctccga catcgcaaa aagcgagcc 
      aagattaga tattttgat aaacaacgc cggaataag 
      ctcctccaa atcaaacag aaccggaga tggtctaca 
      cccggttat gcaaatcaa ttatttcgg ccacttctt 
      gttgacgca tttattgat tgatttact gaagaaatc 
      ggcaccaag tcgtatagt tttcaccag ctctttcat 
      gtcgttttt ccacaactt gaccctcac aaacctcaa 
      ctatgacgc agacaacgg gtttgcctc cgctgcgaa 
      aatttactg caactcgaa actggctca aatgatcgc 
      ttccgacat ttttgccgc gaaattgaa aggaaccgg 
      cgttacttc aaactcgat caacccggg gattgtcaa 
      caccacgct tttggcaca atttttcac tgtgcaaac 
      gcagctcaa acctttaca gctcatcac agatttgta 
      tttgcgatt tgtggccaa agacgcttg ggagggttc 
      gcaagtcat tgtgcattg cgcggtttc aaaaaaact 
      ggaaggcgt gagcgggcg gtatttctg gaattgtgt 
      gcccgtttt taagccttt gcaggctca ggatggcga 
      gttttgcag gaaaatttg gggcgaaac ggagagact 
      cgtaaagtt ggcgcccga agagaggct ttgattttt 
      gtgccaaaa aagccaccg ataaattgt gttaacgat 
      tttgtaata cagttaatt tgttacaac taattaaat 
      aaaggaaat cagaacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY