Status
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Gene information
General information
TC034449
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011728
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14372079..14413581
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034449
    iBeetle screen iB_05092

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature lateral number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs pretarsus swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034449 RA
      MYNNHHLAA PSTLIVNRY TEINYFDNP QAANLEKYV 
      YDMHTSQDV PCISAFASL LFALIPAAD PYHIFIPFS 
      SCLRQFVRP VRTDYRVLN ARILHSKLN FFCFYLLQR 
      GSEELLSQS NVSVMAPSA PHADNNNQE NAKKVKLDQ 
      QNNVGKPSR VIHIRNIPN DVSEAEIVH LGIPFGRVT 
      NVLVLKGKN QAFLEMGEE SSATTMVNY FSSSMAQLR 
      GRAVYVQYS NHKELKTDQ THSNANASA QAALQAAQA 
      LTSTQNSIT PTSTPATPV TNGQDIQGG PNTVLRVII 
      EHMIYPISL DILHLIFQR FGKVLKIVT FTKNNSFQA 
      LIQYPDTAS AQSAKQALD GQNIYNSCC TLRIDYSKM 
      SSLNVKYNN DKSRDYTNP NLPTGDAND QLATLGGGL 
      AGGLGADIL LLAAQPRLA RERMADSIL AGTPGVLSG 
      PFMHGLAST LATPYGGGV AGGIPGLGG FALGQTAPG 
      LRGIAAPGL ALATVLLVS NLNEETVTP DALFTLFGV 
      YGDVQRVKI LYNKKDSAL VQLAEPHQA HLAITHMDK 
      LKVFGKTIR VMLSKHQSV QMPKEGQPD AGLTKDYSQ 
      SPLHRFKKP GSKNYQNIY PPSSTLHLS NIPATINED 
      DIKEAFTKN GFTVKAFKF FPKDKKMAL IQLPSMEEA 
      VEALIRMHN YQLSESNHL RVSFSKSSI
    CDS sequence
    • >TC034449 RA
      atgtacaat aaccatcac ctggccgcc ccgagtacg 
      ctcatcgtc aatcgatac actgaaatc aactacttc 
      gacaatccg caagctgcc aacttggaa aagtacgtc 
      tacgacatg cacacatcc caagatgta ccgtgtata 
      agcgctttt gcgtcactt ctcttcgcc cttattccc 
      gccgccgac ccttatcac atattcatc ccgttttcc 
      tcttgcctc cgacaattt gttcgcccc gtacgaacg 
      gattatcga gtcttgaat gctcgcatc ttgcactcg 
      aaactcaat tttttctgt ttttatttg ttgcagaga 
      ggaagcgaa gaactgctg tcgcagtcg aacgtctca 
      gtgatggct ccttcagcc ccgcatgcg gacaacaac 
      aatcaggaa aatgccaaa aaggtgaag ttggaccaa 
      cagaacaac gtcggtaaa ccgtcgcgt gttattcac 
      atccgtaac attccaaac gacgtatcg gaagccgaa 
      attgttcat ctgggaata cctttcgga agagtaacg 
      aacgtttta gtgctcaaa gggaagaat caggcgttc 
      ttagaaatg ggcgaggaa tcctctgcc acaacaatg 
      gtcaattat ttctccagc tcgatggcg cagctgaga 
      ggaagagcg gtctatgta caatactcg aatcataag 
      gaactcaag acggaccaa acacattcg aatgcgaat 
      gcgtcagct caagctgcc ctccaggcc gcccaagcg 
      ctgacaagt acccaaaac tcgataacg cccacttca 
      actcctgcc acaccagtc accaatgga caagacatc 
      caagggggg cctaacacg gtgttgcga gtcatcatc 
      gagcacatg atttatccc ataagtcta gatattttg 
      catttgata ttccaacgt ttcggaaaa gttctcaaa 
      atcgtcacc ttcaccaaa aataactca ttccaggcg 
      ctaatccag tacccggac acggcgtcg gcccagtcg 
      gccaagcag gccctcgac ggccagaac atctacaac 
      agctgttgc acgctgcgc atcgactac tcgaagatg 
      tcgtcgttg aacgtgaaa tacaacaac gacaaatcg 
      cgcgactac accaacccc aacctgccc accggcgac 
      gccaacgac cagctcgcc accctcggc ggcggcctc 
      gccggcggc ctgggggcg gacatcctg ctgctggcg 
      gcgcagccc cgcttggcg cgcgaacgc atggccgac 
      tccatcctg gccgggacg ccgggcgtt ctgagcggg 
      ccgttcatg cacggcctg gcgtcgacg ctggcgacg 
      ccctacgga ggcggcgtc gccggcggc atccccggc 
      ctcgggggc ttcgccctg gggcagacg gcgcccgga 
      ctgcggggc atcgcggcg cccggattg gcactcgcc 
      accgtctta ctcgttagt aatttaaac gaagagacg 
      gtcacgcct gatgctctc tttacacta tttggggtg 
      tacggtgac gtgcaaagg gtgaagatc ttgtacaac 
      aagaaggat tcggctctg gtccagttg gcggagcct 
      caccaagca cacttggct ataacgcac atggataag 
      ctgaaggtg ttcggaaag acaatcagg gtaatgttg 
      agcaagcac cagtcggtg cagatgccg aaagaaggc 
      cagccggac gctggcctc acaaaagac tacagccag 
      agtccgctg cacaggttt aagaagcca ggctcgaag 
      aattaccag aatatctat ccgccctcg tcgacgctg 
      catctcagt aatataccg gcgacgatc aacgaggac 
      gatatcaaa gaggctttc accaagaac gggtttaca 
      gtaaaggcc ttcaaattc ttccccaaa gacaagaag 
      atggcgctg atccagctg ccgagcatg gaggaggcc 
      gtggaggcc ctgatccgg atgcacaac taccaactt 
      tccgaatcg aatcacttg cgagtctcc ttttcgaaa 
      tcgagtata taa
    mRNA sequence
    • >TC034449 RA
      attgttttt gacaatcgt gcaatatcg caaaggata 
      ccaaatatc aattattga agacaatcg gtcgtaaca 
      ttctggtga caatcgtta gggtcgtga taaagtaac 
      ttagtgatc tgaaagtag aatccgaac atataccac 
      catcaccac gacgtactt cctgaacgc ccccggcca 
      aatccctta cgtagtgcc cttgtgtcc tttcgtacc 
      ctgtcaaga gatcgccgc tccacctag tgcttacgc 
      cccgtgtcc cgtgccctg tccctgtgg ccaatgtgc 
      ccccccgca catgtacaa taaccatca cctggccgc 
      cccgagtac gctcatcgt caatcgata cactgaaat 
      caactactt cgacaatcc gcaagctgc caacttgga 
      aaagtacgt ctacgacat gcacacatc ccaagatgt 
      accgtgtat aagcgcttt tgcgtcact tctcttcgc 
      ccttattcc cgccgccga cccttatca catattcat 
      cccgttttc ctcttgcct ccgacaatt tgttcgccc 
      cgtacgaac ggattatcg agtcttgaa tgctcgcat 
      cttgcactc gaaactcaa ttttttctg tttttattt 
      gttgcagag aggaagcga agaactgct gtcgcagtc 
      gaacgtctc agtgatggc tccttcagc cccgcatgc 
      ggacaacaa caatcagga aaatgccaa aaaggtgaa 
      gttggacca acagaacaa cgtcggtaa accgtcgcg 
      tgttattca catccgtaa cattccaaa cgacgtatc 
      ggaagccga aattgttca tctgggaat acctttcgg 
      aagagtaac gaacgtttt agtgctcaa agggaagaa 
      tcaggcgtt cttagaaat gggcgagga atcctctgc 
      cacaacaat ggtcaatta tttctccag ctcgatggc 
      gcagctgag aggaagagc ggtctatgt acaatactc 
      gaatcataa ggaactcaa gacggacca aacacattc 
      gaatgcgaa tgcgtcagc tcaagctgc cctccaggc 
      cgcccaagc gctgacaag tacccaaaa ctcgataac 
      gcccacttc aactcctgc cacaccagt caccaatgg 
      acaagacat ccaaggggg gcctaacac ggtgttgcg 
      agtcatcat cgagcacat gatttatcc cataagtct 
      agatatttt gcatttgat attccaacg tttcggaaa 
      agttctcaa aatcgtcac cttcaccaa aaataactc 
      attccaggc gctaatcca gtacccgga cacggcgtc 
      ggcccagtc ggccaagca ggccctcga cggccagaa 
      catctacaa cagctgttg cacgctgcg catcgacta 
      ctcgaagat gtcgtcgtt gaacgtgaa atacaacaa 
      cgacaaatc gcgcgacta caccaaccc caacctgcc 
      caccggcga cgccaacga ccagctcgc caccctcgg 
      cggcggcct cgccggcgg cctgggggc ggacatcct 
      gctgctggc ggcgcagcc ccgcttggc gcgcgaacg 
      catggccga ctccatcct ggccgggac gccgggcgt 
      tctgagcgg gccgttcat gcacggcct ggcgtcgac 
      gctggcgac gccctacgg aggcggcgt cgccggcgg 
      catccccgg cctcggggg cttcgccct ggggcagac 
      ggcgcccgg actgcgggg catcgcggc gcccggatt 
      ggcactcgc caccgtctt actcgttag taatttaaa 
      cgaagagac ggtcacgcc tgatgctct ctttacact 
      atttggggt gtacggtga cgtgcaaag ggtgaagat 
      cttgtacaa caagaagga ttcggctct ggtccagtt 
      ggcggagcc tcaccaagc acacttggc tataacgca 
      catggataa gctgaaggt gttcggaaa gacaatcag 
      ggtaatgtt gagcaagca ccagtcggt gcagatgcc 
      gaaagaagg ccagccgga cgctggcct cacaaaaga 
      ctacagcca gagtccgct gcacaggtt taagaagcc 
      aggctcgaa gaattacca gaatatcta tccgccctc 
      gtcgacgct gcatctcag taatatacc ggcgacgat 
      caacgagga cgatatcaa agaggcttt caccaagaa 
      cgggtttac agtaaaggc cttcaaatt cttccccaa 
      agacaagaa gatggcgct gatccagct gccgagcat 
      ggaggaggc cgtggaggc cctgatccg gatgcacaa 
      ctaccaact ttccgaatc gaatcactt gcgagtctc 
      cttttcgaa atcgagtat ataatgcag cgtttaaga 
      agaagaaaa aaaaacaat ccgattgca cagtacagt 
      attcattcc atttgtcgt agcgacgac attagctaa 
      tatattata taattgcag cgcttattt attgaatag 
      actgtttct ggcaggtta agctttgat tgatgattc 
      tattgacaa atcagtatg acgttagta tttttttaa 
      agagatgtt agttataag ttgtatttt ctaagtatt 
      gttgaagaa ggaagtatt tttttattt tatgacgat 
      gatatttag ttgcgttgc gttcagtat tggatggct 
      ttaaattca aaccgctac aaaacgaga gaaacatag 
      gtgattatg tggatcaag tcgccgcga tgttatttt 
      ttgtaagtt tatagagag tgttagtgt taaggcggg 
      ttgttcacc gaccacggc ctactagta ttaattggc 
      tagtattgt taggacgtc gcggacaaa ttagcgtat 
      cgtagtttt agccagtga ttagtttag ggtaaacca 
      cagggttct agatgtacc ctagagata tggatgtgt 
      tgtcttgta ggtccaaga aaacgattc atattttgc 
      ggagagatc aagccaccg tgtccccgt attatactt 
      acaatgaat ctctttatt ttttctaaa acacgctcg 
      cttgatctc cggtttatt aattatata acggtatat 
      atttctctc ttatcttaa ctatacact tgacaatca 
      tagattcat tactattta gtgaaaaca aagacgcgt 
      gattcgctg tctcttctc atccaatca aaatcgtgc 
      gcacgcgct tgcgcagtg acaagggac atatcgcat 
      ggatgttat ttgccaaat tcaaagtca atatctaca 
      atacttgtg actacacta aatgtctat aaaaaacga 
      tattttttc atatgttaa gtgcgcaca agtctttca 
      cgacgaggt cgcttttgt tcgaatcgc ttctcagga 
      ttttatttc atttccttt cgttagtgt gtaatttat 
      gcatttact gtttatttt tttctgttt gataattaa 
      actttttct ttgtattta tacatttcg tttatttta 
      cccgagttt tttccgcac cacagagga ctaaaacat 
      gctggaaaa atggcccaa aaagtatta aatgacgag 
      taaaatttt ctggttcaa cagctaaat cacgaaaac 
      agataaagc agagtaata cagtataaa gttaagctc 
      aaggaattt aaaatctgg gaaatagtc gtttaaaaa 
      aacaagtta aagagaagt tgagccgaa attcaaacc 
      tctaaaaat tcaactaaa atcgttaat ctcggaaac 
      attaaaaaa caaatcaaa aaaaattaa attaaattc 
      caccctttc ttaataata caggattca gtaaaaact 
      tacttttat cgaactgtc ttggcatac aatttttga 
      caaaagaga ttaattggc gcagtcggt aggatttat 
      ggcgcaatt ttaagaggt tggaatggt ggctgttta 
      ggacctaaa cctgcgatt taaaccgct tgtcgcttt 
      agtcctcgg tggtttctt ccatacaaa tattgtaaa 
      accggactt ggctgtcgt caggtctgg tttgcgagg 
      cttgtagaa aagtctcag ttttgtgat tgatattta 
      tgagcgtga actggcaca cgccataat aggaacaaa 
      gatggcatc gtgcctgac cgtgggatc aactaaaca 
      aatcattga ataaatatc tatttaaca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY