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Gene information
General information
TC011724 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14495592..14509031
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011724
    iBeetle screen iB_05091

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011724 RA
      MPDAIMKPI YLRGLLWLY LLGCFLFVA ALEPGFLDF 
      DNLPDTNFS CVGKVIGGY YADLETNCQ MFHVCTIGQ 
      LDEPMDIRF LCLNGTVFD QETRVCERI DEVDCSKSE 
      QFYSLNLEL YGNTQPSVL EESETEPPI IIKASTTTT 
      TTTPRPKPK TTRTYYTTS APTVTTVTK VISSHHFPI 
      NGQTDIRFN PEEINISLN PGAPPNIRK PVTYHQQVF 
      SDNKKVVVT THTTFGTDK EDILRVVPN TQRPVSSTQ 
      KAKPPATPA DINYGFTYH QTEKVPHNY QYHHANFHQ 
      HGQFRDQFK TTERATTRK HPYKEPTTF RTTYVKPQY 
      QYRTEKPQR VQLPVPLLP TLPPLTFSS PAPFSLGHH 
      IEAKRYTND HQSPRIIIS ASASVSDAS GRRLNYSLG 
      TIGVTPLLE QPPKTYDEY KDHDVVLDP FYHDVPKIK 
      NKRRKRQVN HSEVIKNEQ EAVDVLKFL FDWYQNHQR 
      TSTIRVPID AEDITEINQ ELAPIVEPP EPINHTKIE 
      PNFGESDLF QRKSKFNIV GDVLSVTEV EDNSTTSTS 
      TSASVGSED YVDDNYEAV QGTKVETTT ISTTSTTRR 
      VRGRGRAHY RKVSESEPK YSASYSRRR GRGRSTTTT 
      EVPPKEEIV EELKKEASL KEESRVVEV SEKSVEETT 
      TKPEETLET TTIGKNSSE ETTTRTDET LETTTIKNN 
      STEDVTKSE EILEITTRT AETTTLKEE TTNKNDEVD 
      ETSSLEDES HLEPETTTV LDEETTEEF QIASETSAA 
      DLKEDETKN LDQETTTIS LVNDEVKNS KEETQILSE 
      TSAVTEETK TVDELQLLS EDQNEETVE AQSEDFDDL 
      DDLEEKSNV TTPSQVSEG PVTEATLVT TTGEVFDEK 
      TTENETTET ITEVTTLEE KESEEIVLS KPKTEDAVS 
      PKIETKNET AEVVLPENP ETTEYLHES EETTFATTD 
      AVATETEFS PTSTSSSTT ENHATVTTS TSTESPNLT 
      PAQTTTETL DIPRGRSAH RRVRPSRPR SYRRPNPRH 
      RFSTELFTS RPTRRKTTV EINSNALTS QLTTTELVS 
      TSPTEEVPP TDAPLFKSA NPTQAESAK ESLHTTPLS 
      MTYKLSVRK EKLKSYIFN CFGKAINKF YSDPRDCRL 
      FHYCTSGYT KNQLLDMKF VCDLGTYFD DEKLICTKE 
      KPHRCL
    CDS sequence
    • >TC011724 RA
      atgcccgac gccatcatg aagccaatc tatcttcga 
      ggtctgctg tggctctac cttttgggc tgcttcctc 
      ttcgtcgcg gccctggaa cctggcttt ttggacttt 
      gataacctc ccagacacg aacttctcc tgcgttggt 
      aaagtaatc ggagggtac tatgccgac ctggagacc 
      aactgccaa atgttccac gtttgtacc atcgggcaa 
      ctggacgaa cccatggac atccggttc ctgtgcctc 
      aatgggacc gtcttcgac caggaaacg cgagtttgc 
      gaacgaatc gatgaagtt gattgctca aaatctgaa 
      cagttctac agtctcaat ctggaattg tacggaaac 
      acccaaccc agcgttttg gaagaaagc gaaaccgaa 
      ccgccaatc ataatcaag gcttcgacc acgacaaca 
      acaacaacc ccacgccca aaacccaaa acaacacgc 
      acctactac acaacatcg gcaccaaca gtaacaacc 
      gtaaccaaa gtcatatca tcgcaccac ttcccgatc 
      aatggtcag acggacatt cgcttcaac ccggaagaa 
      atcaacatt agtctaaat ccgggggcg ccgccaaac 
      atccggaaa ccagtcacc taccaccag caagtcttc 
      agtgacaat aaaaaagta gtggtaacc actcacaca 
      acttttggt actgataaa gaggatatc cttcgagtg 
      gtaccgaac acccagcgc ccggtctct tcgacccaa 
      aaggcaaaa ccaccggcc acacccgct gacattaat 
      tacgggttc acgtaccat caaactgag aaagtacca 
      cataattat cagtaccat cacgcgaac ttccatcag 
      catggccag ttccgggat cagtttaaa acaaccgag 
      cgtgcaaca acccggaaa cacccatac aaggaacct 
      acgacgttt cgaaccact tatgtcaaa cctcagtac 
      cagtaccgc accgagaag ccacaaagg gtgcaatta 
      ccggtacca ctactacca acactgcca cctttgacg 
      tttagttcc ccagcaccg ttcagtcta ggccaccat 
      atcgaggcc aaacggtac accaacgac catcaatca 
      cccagaatc ataatcagt gctagtgct agtgttagc 
      gatgccagt ggtcgaaga cttaactat tcgctagga 
      accatcggt gtaacgcct ctactagag cagccgccg 
      aaaacctac gatgagtat aaggaccat gacgtggtc 
      ctggaccca ttctaccac gacgtgccc aaaatcaag 
      aacaagaga cgcaagaga caagtcaat cattccgaa 
      gtgatcaaa aacgagcaa gaagcagtc gatgttttg 
      aagtttttg ttcgactgg taccaaaac caccaacgg 
      actagtacc atcagagta ccaatcgat gccgaggac 
      ataacggag ataaaccaa gagttggca cccattgtg 
      gaaccacca gaaccaata aatcacacg aaaattgag 
      cctaacttt ggcgaaagt gacttattc caaagaaaa 
      agcaagttt aacatagtt ggggatgtc ttaagtgtg 
      accgaagtt gaagacaat agtacaact agtacaagt 
      actagtgca agtgtgggt agtgaagac tacgttgat 
      gacaattac gaggcggtt caaggaact aaagttgaa 
      actacaacg atcagtact acaagtacc acaagaagg 
      gtacggggg aggggtcgt gctcattat aggaaggtt 
      tcggaaagt gagccaaaa tactcggct tcgtactcg 
      agacgaaga gggcgaggg agaagtact acgacaact 
      gaagtacca ccaaaagaa gaaattgtt gaagagttg 
      aagaaagaa gcgagtttg aaagaagaa tcgcgagtt 
      gttgaagtt tccgagaaa agtgttgaa gaaacgacc 
      acaaaacca gaagaaact ttagaaaca acaacaatt 
      gggaagaat agtagtgaa gaaacaacc acaaggaca 
      gacgagact ttagaaaca acaacaatt aaaaataac 
      agtactgaa gacgtaacg aaatcagaa gagattttg 
      gaaataacg acaagaacc gcggaaaca acaacactt 
      aaagaagaa acgacaaat aaaaatgat gaagttgac 
      gaaacgtct tcactggaa gatgagtcg catctagaa 
      ccagaaaca acaacagtg cttgatgaa gaaacaacg 
      gaagaattt cagattgct tctgaaacg tcagcagct 
      gatctgaaa gaagatgaa accaagaac ctggaccaa 
      gaaacgaca acaatttcg ttagttaac gatgaagta 
      aagaattcg aaggaagaa acgcagata ttgtctgaa 
      acgtcagca gttaccgaa gaaacaaag actgtggat 
      gaactgcag ttactttcc gaagaccag aatgaagaa 
      acggtggaa gcacaaagc gaagatttt gatgacttg 
      gatgattta gaagaaaaa tcaaatgtt acgacacca 
      agtcaagtc agtgaaggt ccagtaact gaagctacg 
      ctagtcaca actactgga gaagttttt gacgaaaag 
      accacagaa aacgaaaca actgaaaca ataacggaa 
      gtgaccaca ctagaagaa aaagaaagt gaagaaata 
      gttttgtct aaacccaaa actgaagat gcggtttcg 
      ccaaaaatc gaaacgaag aatgaaact gcagaagtg 
      gttttgcct gaaaatcct gaaacgaca gagtatttg 
      cacgaaagc gaagaaacg acttttgcg accactgac 
      gctgtcgca actgaaacc gaattttca cctactagt 
      accagttct agtactact gaaaatcac gcaactgtg 
      accactagc actagtacc gagtccccg aacttgacc 
      cctgctcaa acaaccacc gaaactttg gacattcct 
      cgtgggcgc agtgcccat cgcagggtg cgcccttcg 
      cgccctaga agttaccgc cgaccaaac cctagacat 
      aggtttagt accgaattg ttcacatct cggcccacg 
      cgccgaaaa accaccgtc gaaatcaac tcgaacgct 
      ttaacaagt caactgacc accactgaa cttgtctct 
      acctcaccc acggaagaa gtccccccc accgacgcc 
      cccttgttc aaaagtgcc aaccctacg caagccgaa 
      tcggccaaa gagtccctc catacgacc cccttgtcg 
      atgacttat aaactaagc gtgcggaag gagaaactc 
      aagtcgtac atttttaac tgtttcggg aaagcgatt 
      aataagttt tactccgat ccgagagac tgtaggctt 
      ttccattat tgtacttcg gggtacacg aaaaaccag 
      ctgctagac atgaagttt gtgtgtgat ttggggact 
      tatttcgac gatgagaag ctcatttgt acaaaggag 
      aagccccac aggtgtttg taa
    mRNA sequence
    • >TC011724 RA
      tatgtaaat cggcgacct ttggcgcgg taatttccc 
      gaaatcggc gcatcttcg gaaggtcaa tcattacat 
      aaaccccga atgtcagcg gtcctccac tttctccaa 
      aaattcctt gtttgcctt tgcaattgc acaaaacct 
      ttggtggcg ccgccccac ttttcctgc ttcctgctg 
      caggaaggc cagttctgg ggtcctgca ggagctgct 
      gaagctcaa gttcagcgc tccgacgac ggtcgacca 
      gctccgagg cggcttcgg tcttcactt tcatatgtc 
      tttggtctg gtaacacac tgcagctat cctcaataa 
      cgctaggtg gacctccgc aggtttttg catttgtgc 
      tgtgcgcga tgtgcttgc ccagctgga tagccttca 
      aggccagtg ataacgtgc agtgataaa ggataatgg 
      atctggatg aagagcggg ttgtcacag cggcttagc 
      gatgcccga cgccatcat gaagccaat ctatcttcg 
      aggtctgct gtggctcta ccttttggg ctgcttcct 
      cttcgtcgc ggccctgga acctggctt tttggactt 
      tgataacct cccagacac gaacttctc ctgcgttgg 
      taaagtaat cggagggta ctatgccga cctggagac 
      caactgcca aatgttcca cgtttgtac catcgggca 
      actggacga acccatgga catccggtt cctgtgcct 
      caatgggac cgtcttcga ccaggaaac gcgagtttg 
      cgaacgaat cgatgaagt tgattgctc aaaatctga 
      acagttcta cagtctcaa tctggaatt gtacggaaa 
      cacccaacc cagcgtttt ggaagaaag cgaaaccga 
      accgccaat cataatcaa ggcttcgac cacgacaac 
      aacaacaac cccacgccc aaaacccaa aacaacacg 
      cacctacta cacaacatc ggcaccaac agtaacaac 
      cgtaaccaa agtcatatc atcgcacca cttcccgat 
      caatggtca gacggacat tcgcttcaa cccggaaga 
      aatcaacat tagtctaaa tccgggggc gccgccaaa 
      catccggaa accagtcac ctaccacca gcaagtctt 
      cagtgacaa taaaaaagt agtggtaac cactcacac 
      aacttttgg tactgataa agaggatat ccttcgagt 
      ggtaccgaa cacccagcg cccggtctc ttcgaccca 
      aaaggcaaa accaccggc cacacccgc tgacattaa 
      ttacgggtt cacgtacca tcaaactga gaaagtacc 
      acataatta tcagtacca tcacgcgaa cttccatca 
      gcatggcca gttccggga tcagtttaa aacaaccga 
      gcgtgcaac aacccggaa acacccata caaggaacc 
      tacgacgtt tcgaaccac ttatgtcaa acctcagta 
      ccagtaccg caccgagaa gccacaaag ggtgcaatt 
      accggtacc actactacc aacactgcc acctttgac 
      gtttagttc cccagcacc gttcagtct aggccacca 
      tatcgaggc caaacggta caccaacga ccatcaatc 
      acccagaat cataatcag tgctagtgc tagtgttag 
      cgatgccag tggtcgaag acttaacta ttcgctagg 
      aaccatcgg tgtaacgcc tctactaga gcagccgcc 
      gaaaaccta cgatgagta taaggacca tgacgtggt 
      cctggaccc attctacca cgacgtgcc caaaatcaa 
      gaacaagag acgcaagag acaagtcaa tcattccga 
      agtgatcaa aaacgagca agaagcagt cgatgtttt 
      gaagttttt gttcgactg gtaccaaaa ccaccaacg 
      gactagtac catcagagt accaatcga tgccgagga 
      cataacgga gataaacca agagttggc acccattgt 
      ggaaccacc agaaccaat aaatcacac gaaaattga 
      gcctaactt tggcgaaag tgacttatt ccaaagaaa 
      aagcaagtt taacatagt tggggatgt cttaagtgt 
      gaccgaagt tgaagacaa tagtacaac tagtacaag 
      tactagtgc aagtgtggg tagtgaaga ctacgttga 
      tgacaatta cgaggcggt tcaaggaac taaagttga 
      aactacaac gatcagtac tacaagtac cacaagaag 
      ggtacgggg gaggggtcg tgctcatta taggaaggt 
      ttcggaaag tgagccaaa atactcggc ttcgtactc 
      gagacgaag agggcgagg gagaagtac tacgacaac 
      tgaagtacc accaaaaga agaaattgt tgaagagtt 
      gaagaaaga agcgagttt gaaagaaga atcgcgagt 
      tgttgaagt ttccgagaa aagtgttga agaaacgac 
      cacaaaacc agaagaaac tttagaaac aacaacaat 
      tgggaagaa tagtagtga agaaacaac cacaaggac 
      agacgagac tttagaaac aacaacaat taaaaataa 
      cagtactga agacgtaac gaaatcaga agagatttt 
      ggaaataac gacaagaac cgcggaaac aacaacact 
      taaagaaga aacgacaaa taaaaatga tgaagttga 
      cgaaacgtc ttcactgga agatgagtc gcatctaga 
      accagaaac aacaacagt gcttgatga agaaacaac 
      ggaagaatt tcagattgc ttctgaaac gtcagcagc 
      tgatctgaa agaagatga aaccaagaa cctggacca 
      agaaacgac aacaatttc gttagttaa cgatgaagt 
      aaagaattc gaaggaaga aacgcagat attgtctga 
      aacgtcagc agttaccga agaaacaaa gactgtgga 
      tgaactgca gttactttc cgaagacca gaatgaaga 
      aacggtgga agcacaaag cgaagattt tgatgactt 
      ggatgattt agaagaaaa atcaaatgt tacgacacc 
      aagtcaagt cagtgaagg tccagtaac tgaagctac 
      gctagtcac aactactgg agaagtttt tgacgaaaa 
      gaccacaga aaacgaaac aactgaaac aataacgga 
      agtgaccac actagaaga aaaagaaag tgaagaaat 
      agttttgtc taaacccaa aactgaaga tgcggtttc 
      gccaaaaat cgaaacgaa gaatgaaac tgcagaagt 
      ggttttgcc tgaaaatcc tgaaacgac agagtattt 
      gcacgaaag cgaagaaac gacttttgc gaccactga 
      cgctgtcgc aactgaaac cgaattttc acctactag 
      taccagttc tagtactac tgaaaatca cgcaactgt 
      gaccactag cactagtac cgagtcccc gaacttgac 
      ccctgctca aacaaccac cgaaacttt ggacattcc 
      tcgtgggcg cagtgccca tcgcagggt gcgcccttc 
      gcgccctag aagttaccg ccgaccaaa ccctagaca 
      taggtttag taccgaatt gttcacatc tcggcccac 
      gcgccgaaa aaccaccgt cgaaatcaa ctcgaacgc 
      tttaacaag tcaactgac caccactga acttgtctc 
      tacctcacc cacggaaga agtcccccc caccgacgc 
      ccccttgtt caaaagtgc caaccctac gcaagccga 
      atcggccaa agagtccct ccatacgac ccccttgtc 
      gatgactta taaactaag cgtgcggaa ggagaaact 
      caagtcgta catttttaa ctgtttcgg gaaagcgat 
      taataagtt ttactccga tccgagaga ctgtaggct 
      tttccatta ttgtacttc ggggtacac gaaaaacca 
      gctgctaga catgaagtt tgtgtgtga tttggggac 
      ttatttcga cgatgagaa gctcatttg tacaaagga 
      gaagcccca caggtgttt gtaaatatg ctctcttag 
      ctagcgatc gaacgtcct attagccta aatcaattt 
      gtaattatt actgaactt gttatgaat actcataga 
      ttaatataa ttaaattaa atcaataaa ctattttgt 
      taaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY