Status
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Gene information
General information
TC011720 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14536488..14547784
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011720
    iBeetle screen iB_05089

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011720 RA
      MSSSSRSNI YENNNDIKA KMAETPQPT PKPCAAKPR 
      PGCCTCLHL NNLWSLWYG VFGTALQAY TAIKCVKRI 
      LGYSLLAWP ENLPVPYLE LNCSLGLTG AAILLLPLF 
      LVATLFKIG NLANDGFKL GRQLSTCSR EPANEILTN 
      NGTCRLFRH GGPTAPFVH LVIAFCLLV PKLLMEARL 
      IEAGFLSQD WIWRTDLDF MGLHKDRMV ILSFMTPNN 
      TTIAQVKRS ATPSPITPL PFTSNSVVT NTVIKTLKD 
      FIGHENHTN FEHKNIETG WGSSVSVEY MNLVIALMV 
      YSIRYPALF WSTNKCLGT IFSFQLLIN GLHTLLAYA 
      GMSILYKVQ IVGGWKTLP LLKHEPTTL FTFSKLSPF 
      LLNPQVTLA LYMMSTLLI LSSSLVLYL YGHTRFNAF 
      LNQERERKV IMLKEGSTN RWSYFTHCA ALCVLVSVG 
      ICNAPLLHD YTVLYKGSL DETTLACIV GGILHLFFW 
      IVIWLFLTI KQTWIFKLR VTVGRATVR QARSLKLVT 
      DIDLMSSRV EDLSQQPLL IVGNGRTYN IAETSPKKA 
      IMSVIQKAT MSKKASNGS VSNQDDDEE QIYWLRPAL 
      VTPQSSPDE AKQLCWFKN KPKHKVTFN ETTSTSVNR 
      SKNSGVRGR RLTAMDEDD GDYATLREL PLPQPVRRP 
      ENREDTVSE EGKLLACVQ DERVTYASN NQDLIPPVD 
      YEDPSPLLT PEPLYAQKT PASVIVHSH ETQITTGQT 
      PRCLRRADS GMPHDELTP RSDTLSTES STSPPEPPG 
      SNHSETSSG VHSNDSDHK TARRATSVV DLVQNREEI 
      HWKSSSLQR NTSPPPSQA VILENPAES TVVIRRKVS 
      RPVPADVVQ GVGVKEEPF GRATNMRMT SFMEKTDLG 
      GMQASSATL PHFPTQPMQ NVYAHCSTM PLPQHGGIP 
      NGQSLVAGN SCNVYPRQH TTLPTHHNG VKLFHPQNP 
      YVTRFKCKQ AEPIKTNIH EPIYTGVNK SGDMYHYNS
    CDS sequence
    • >TC011720 RA
      atgtcttca tcctctcgc agcaacatt tacgagaac 
      aacaatgac ataaaagcg aaaatggca gagacgccg 
      caaccgacg cccaagccc tgcgccgcc aagccccgg 
      cccggctgc tgcacttgc ctccacctc aacaacttg 
      tggtcgctg tggtacggc gtcttcgga acggccttg 
      caggcctac accgcgatc aagtgcgtc aagcggatc 
      ctggggtac tcgctgttg gcctggccg gagaacctg 
      ccagtgccg tacttggag ctgaactgc agtctgggc 
      ctgacaggg gccgcgatt ctgctgctg cccctgttc 
      ctggtcgcg acgctgttc aaaatcggg aacttggcc 
      aatgacggg ttcaagctg ggacggcag ttgagcacg 
      tgcagcagg gagccggcg aacgagatt ctgacgaac 
      aatgggact tgtcggctg tttaggcat gggggaccc 
      acggcgccg tttgtgcac ttggtcatt gcgttttgt 
      ctgctggtg cccaagctg ctgatggag gccaggctg 
      attgaagcc gggttcttg tcacaagac tggatctgg 
      cgcacggat ttggacttc atgggtctg cacaaagac 
      cgcatggta attttaagc ttcatgaca cccaacaac 
      acgacaata gcgcaagtc aaacgctcg gcgacccct 
      tcacccatc actcctctc ccatttacc agcaactcg 
      gttgtgact aatactgta ataaagact ttgaaggat 
      tttatcggt catgagaac cacacgaat ttcgaacac 
      aaaaatatc gaaacagga tggggcagt tcagttagt 
      gtggagtac atgaatctg gtcatagct ctaatggtg 
      tactccata cggtatccg gcgttgttc tggtcgacg 
      aacaagtgt ctgggtaca attttctcg tttcagctg 
      ctgatcaac ggcttacat acgcttttg gcctacgcc 
      ggaatgtcc atcttatac aaagtacaa atcgtcggc 
      gggtggaaa accctccct ttgctcaaa cacgaaccg 
      acgactttg ttcacattt agcaaatta tcaccgttt 
      ttactcaac ccacaagtt actctagca ctttacatg 
      atgtcaacg cttctaatt ctatcgtct agcctagtg 
      ttgtatctt tatggccat acgagattc aacgcgttc 
      ctgaaccag gaacgcgaa cgaaaagtc ataatgttg 
      aaggaaggg agcaccaac aggtggagc tacttcacc 
      cattgtgcc gccctgtgt gtcttggta tcggtaggg 
      atatgcaac gcccccctc ctccatgac tacaccgtc 
      ctgtacaaa ggaagtctg gatgaaacc acgttggct 
      tgcatcgtc ggcggaatc ctccacctg tttttctgg 
      atcgtcatc tggctattt ttgacaata aaacaaacc 
      tggattttt aaactgaga gttacagtc ggccgagca 
      acagtgcgc caagcccgc tccctgaaa ctcgtcacc 
      gacatcgat ttaatgtcc tccagggtt gaggatctc 
      tcgcagcaa cccctcctg attgtgggc aacggtcgc 
      acctacaac atcgccgag acctcgccc aagaaagcc 
      ataatgagc gtcatccag aaagccacc atgagcaaa 
      aaagcctcc aatggttcg gtctcgaac caggacgac 
      gacgaggag cagatttac tggcttcgg ccggctcta 
      gtcaccccg cagtcgtca ccagacgag gccaagcag 
      ttgtgctgg ttcaagaac aagccgaag cacaaagtc 
      accttcaac gaaacaact agtacgtct gttaatcgt 
      agtaaaaac agtggagta cgaggacga cgattgacg 
      gcgatggac gaggatgac ggggattat gccacgctg 
      agggagctg ccgctccct cagccggtt cgcaggccg 
      gagaaccgc gaagatacg gtcagtgaa gagggaaag 
      cttctggct tgcgtgcaa gacgaacgt gtaacatac 
      gctagtaat aatcaagat ctaatacct cctgtagat 
      tatgaggat ccgagtcct ttactaaca ccagaacca 
      ttatacgca caaaaaaca cccgcatct gtcattgtt 
      cacagtcat gagacgcag atcaccact ggccaaacc 
      ccccgttgc ctccgtcgc gccgactcg ggcatgccc 
      cacgacgag ctcaccccc cgttccgac accctcagc 
      accgagagc tccacgtcc cctcccgag cccccaggg 
      agcaaccac agcgagacc tccagcggc gtccactcc 
      aacgacagc gaccacaaa accgcccgt cgtgccacc 
      agcgtcgta gacctcgtc caaaaccgg gaggaaatc 
      cactggaag tcctcctcc ctccaacgc aacacgagc 
      cctccaccc agccaggca gtcatcctg gaaaacccc 
      gccgagagc acggtggta atcaggagg aaggtctcg 
      cggccggtc cctgcagac gtcgtccag ggcgtgggc 
      gtgaaggag gagccgttc gggagggcg accaacatg 
      cggatgacc tccttcatg gagaagact gacctgggg 
      ggcatgcag gcctcgtct gccacgctg ccgcacttc 
      cccacccag cccatgcag aacgtctac gcccactgc 
      tcgaccatg ccgctaccc cagcatggg gggatcccc 
      aacgggcag agtctagtc gctgggaac agctgcaat 
      gtgtacccc aggcaacac accaccctc ccgactcat 
      cataacgga gtcaagctg tttcatccc caaaacccc 
      tacgtgacg aggtttaaa tgcaagcaa gccgaacca 
      atcaaaacc aatatacat gagccgatc tacacagga 
      gtgaacaaa tcaggagac atgtatcat tataattct 
      taa
    mRNA sequence
    • >TC011720 RA
      gatcaaaac taacttccg gcgacgctc gcgacgcct 
      gaatagacg gcttggcgc gttatggaa gcgtcggca 
      tctttggca gaaggtcga gtgagcaca agtgtcctt 
      gtgatggtt taattgaga tgtgaacag cgagaacca 
      gttgcgctg ctaaaatga cgtctttag ggcgctgct 
      ccactccca ccgccactg ccaccaagt gagccgtca 
      tgtcttcat cctctcgca gcaacattt acgagaaca 
      acaatgaca taaaagcga aaatggcag agacgccgc 
      aaccgacgc ccaagccct gcgccgcca agccccggc 
      ccggctgct gcacttgcc tccacctca acaacttgt 
      ggtcgctgt ggtacggcg tcttcggaa cggccttgc 
      aggcctaca ccgcgatca agtgcgtca agcggatcc 
      tggggtact cgctgttgg cctggccgg agaacctgc 
      cagtgccgt acttggagc tgaactgca gtctgggcc 
      tgacagggg ccgcgattc tgctgctgc ccctgttcc 
      tggtcgcga cgctgttca aaatcggga acttggcca 
      atgacgggt tcaagctgg gacggcagt tgagcacgt 
      gcagcaggg agccggcga acgagattc tgacgaaca 
      atgggactt gtcggctgt ttaggcatg ggggaccca 
      cggcgccgt ttgtgcact tggtcattg cgttttgtc 
      tgctggtgc ccaagctgc tgatggagg ccaggctga 
      ttgaagccg ggttcttgt cacaagact ggatctggc 
      gcacggatt tggacttca tgggtctgc acaaagacc 
      gcatggtaa ttttaagct tcatgacac ccaacaaca 
      cgacaatag cgcaagtca aacgctcgg cgacccctt 
      cacccatca ctcctctcc catttacca gcaactcgg 
      ttgtgacta atactgtaa taaagactt tgaaggatt 
      ttatcggtc atgagaacc acacgaatt tcgaacaca 
      aaaatatcg aaacaggat ggggcagtt cagttagtg 
      tggagtaca tgaatctgg tcatagctc taatggtgt 
      actccatac ggtatccgg cgttgttct ggtcgacga 
      acaagtgtc tgggtacaa ttttctcgt ttcagctgc 
      tgatcaacg gcttacata cgcttttgg cctacgccg 
      gaatgtcca tcttataca aagtacaaa tcgtcggcg 
      ggtggaaaa ccctccctt tgctcaaac acgaaccga 
      cgactttgt tcacattta gcaaattat caccgtttt 
      tactcaacc cacaagtta ctctagcac tttacatga 
      tgtcaacgc ttctaattc tatcgtcta gcctagtgt 
      tgtatcttt atggccata cgagattca acgcgttcc 
      tgaaccagg aacgcgaac gaaaagtca taatgttga 
      aggaaggga gcaccaaca ggtggagct acttcaccc 
      attgtgccg ccctgtgtg tcttggtat cggtaggga 
      tatgcaacg cccccctcc tccatgact acaccgtcc 
      tgtacaaag gaagtctgg atgaaacca cgttggctt 
      gcatcgtcg gcggaatcc tccacctgt ttttctgga 
      tcgtcatct ggctatttt tgacaataa aacaaacct 
      ggattttta aactgagag ttacagtcg gccgagcaa 
      cagtgcgcc aagcccgct ccctgaaac tcgtcaccg 
      acatcgatt taatgtcct ccagggttg aggatctct 
      cgcagcaac ccctcctga ttgtgggca acggtcgca 
      cctacaaca tcgccgaga cctcgccca agaaagcca 
      taatgagcg tcatccaga aagccacca tgagcaaaa 
      aagcctcca atggttcgg tctcgaacc aggacgacg 
      acgaggagc agatttact ggcttcggc cggctctag 
      tcaccccgc agtcgtcac cagacgagg ccaagcagt 
      tgtgctggt tcaagaaca agccgaagc acaaagtca 
      ccttcaacg aaacaacta gtacgtctg ttaatcgta 
      gtaaaaaca gtggagtac gaggacgac gattgacgg 
      cgatggacg aggatgacg gggattatg ccacgctga 
      gggagctgc cgctccctc agccggttc gcaggccgg 
      agaaccgcg aagatacgg tcagtgaag agggaaagc 
      ttctggctt gcgtgcaag acgaacgtg taacatacg 
      ctagtaata atcaagatc taatacctc ctgtagatt 
      atgaggatc cgagtcctt tactaacac cagaaccat 
      tatacgcac aaaaaacac ccgcatctg tcattgttc 
      acagtcatg agacgcaga tcaccactg gccaaaccc 
      cccgttgcc tccgtcgcg ccgactcgg gcatgcccc 
      acgacgagc tcacccccc gttccgaca ccctcagca 
      ccgagagct ccacgtccc ctcccgagc ccccaggga 
      gcaaccaca gcgagacct ccagcggcg tccactcca 
      acgacagcg accacaaaa ccgcccgtc gtgccacca 
      gcgtcgtag acctcgtcc aaaaccggg aggaaatcc 
      actggaagt cctcctccc tccaacgca acacgagcc 
      ctccaccca gccaggcag tcatcctgg aaaaccccg 
      ccgagagca cggtggtaa tcaggagga aggtctcgc 
      ggccggtcc ctgcagacg tcgtccagg gcgtgggcg 
      tgaaggagg agccgttcg ggagggcga ccaacatgc 
      ggatgacct ccttcatgg agaagactg acctggggg 
      gcatgcagg cctcgtctg ccacgctgc cgcacttcc 
      ccacccagc ccatgcaga acgtctacg cccactgct 
      cgaccatgc cgctacccc agcatgggg ggatcccca 
      acgggcaga gtctagtcg ctgggaaca gctgcaatg 
      tgtacccca ggcaacaca ccaccctcc cgactcatc 
      ataacggag tcaagctgt ttcatcccc aaaacccct 
      acgtgacga ggtttaaat gcaagcaag ccgaaccaa 
      tcaaaacca atatacatg agccgatct acacaggag 
      tgaacaaat caggagaca tgtatcatt ataattctt 
      aaaatcgag gaccacgtg catttaaca ctcacatac 
      ttatcactt gttcatatc gtaccactc ttatcatca 
      caacaagac acaataatt ttaggttta ggttttata 
      tggacaagt atacacttg gtagctagg attaaattt 
      tgtatatat acagtagtt aaaataatt ttatatgat 
      tagtagagt cgttttttt attaatgtg gtcaggtca 
      ggttctagt cattaggtt tacgagacg gagaaatat 
      tttatttat cagcttttt atggtaaac ttgttcgtg 
      aaaccccat agacccatg tgtaacagt aacttatat 
      caaaatgtc gaatcaata aacgcatta cactcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY