Status
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Gene information
General information
TC011713 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14589848..14600264
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011713
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05087

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011713 RA
      MVRSRTPSQ GGGSQGFEM ERKYSVPSN PDSRAFSGT 
      TSEDLHAWS IYRQNLNSD FTDSALGST DKSPLPYGN 
      FQLRDTTVQ SILSHPRYG PKSALGSNM YTYLKFGLP 
      RVFPPNHNG SNRSGTPQH FVRNASTRP HTRGPKSRG 
      LRDGSSGYD SSDNETSNY KHNRKYRSD PDFRMQNIH 
      YHGGSPGIP LAAMQQGGV RTHNQWNRN KSVSEANLL 
      AAEYGPHPH HRRNSVAEF GHHHDVVDS HIGRPVSKA 
      ESHISIPVS RRGPSVIRT DYLSQDEDP GTSFIYPHL 
      QVGGIGVWP KTSSCFGSK HHLLLNDVS FEIRGGEIM 
      AIMATSEEE GTALLDLVA GHAKPVVGD IILNGQTVK 
      SEMLKNRVA YVQSDSHLC KDMTVIQTL RFHYDLKKP 
      TDKLGYLKI DAMDRINVL IEDLGLEQV RNTKVASMT 
      ISERRRLNV ACHLLLDTD VVVLDQPTR GMDIFDTFF 
      LVEYLRQWA NGGAGNALG RIVILTLHP PTYEIFTML 
      SRILLISAG RTMFSGRRR DMLPYFALV DYPCPSFKN 
      PSDYYLDLV TLDDLSAEA MLESSQRIE QLAEVFRQK 
      QEPMSDPGP PSTLPMTVR NSNFLVQGF VLFTKSMIY 
      TQPATFITW LTLIVISAS LSLIIGAIF WDVPNTDPQ 
      LNLNDRLGY HYSVMCVMS WPLLLLLTL SEVQRNKNT 
      VERDIKDGL YTRLTYIVS KSIINLPPS LFIWLIYLV 
      PSYSMSGLY MQSLNDYEG FYVYIGLML LYLITIQEF 
      LLAFIYFIP LRDTAATIS ALFLTAFFL SSGYLVHFK 
      DLPPYVKFL QFLSPPTWT MPYLLSREL SPEAIASSS 
      ALMYCRNKQ VQHQDIIVQ LPCPTGNGT QVLTNYGYL 
      TSSLKFYNY GNPPIALVV FYVICFVVA CFAFVCRRT 
      GSQRRARND ANKP
    CDS sequence
    • >TC011713 RA
      atggtgcga agtcgcacc ccttcgcag gggggtggc 
      tcgcaaggc ttcgaaatg gaacgaaag tactcggta 
      ccatcgaac ccagattcg agagctttc tcaggcacc 
      accagcgag gacctccac gcctggtcc atctacaga 
      caaaacctc aactcggat ttcacggac agtgcctta 
      ggctccacc gacaaaagc cccctgcct tatggcaac 
      ttccagctg cgcgacacc acggtccag tccatatta 
      tcccaccct cgatacggc ccaaaatcg gctttgggc 
      tcgaacatg tacacgtat ttaaagttc gggctccct 
      cgtgtcttc ccgccgaac cacaacggc tccaaccgg 
      tcggggact ccccagcat ttcgtccga aatgcctcg 
      acacgtccc cacactagg ggtccaaaa tctcgcggt 
      ttgcgagac ggaagttcc ggttacgac agttcggat 
      aatgagacg tccaattac aaacataac cggaaatac 
      cgctcggat ccagacttc cggatgcag aacattcac 
      tatcacggg ggaagtccc ggaattcct ctcgcggca 
      atgcaacaa ggcggggtc cggactcac aatcaatgg 
      aaccggaat aaaagcgtt tctgaggcc aatttactg 
      gcggctgag tacgggccg cacccgcac cataggagg 
      aacagtgtg gcggagttt gggcaccac catgatgtg 
      gtggacagt cacattggc cgtcctgtg tcgaaggcc 
      gagagccac atctcaatt cccgtctcc cggagaggt 
      ccctcggtc atccgaacg gactacttg tcccaggac 
      gaggacccc ggaacctcc ttcatctac ccgcacctc 
      caggtcggt ggcattgga gtttggccg aaaaccagc 
      tcctgcttc gggtccaag caccacctg ctgctcaac 
      gacgtctcg ttcgagatc cgtggaggc gagatcatg 
      gccatcatg gccacgtcg gaggaagag ggcacagct 
      ctgctggac ctggtggca ggacacgcc aagcccgtc 
      gtgggcgac atcatcctc aacggccag accgtcaaa 
      tcggagatg ttgaagaac cgggtcgcg tacgtccaa 
      agtgactcg catttgtgc aaagacatg accgttatt 
      cagactttg cggtttcac tacgatctc aaaaaaccc 
      accgataag ttgggctat ctcaagatc gatgccatg 
      gaccggatc aacgtgctg atcgaggat ctgggcttg 
      gagcaggtg aggaacacc aaagtggcg tcgatgacg 
      atttcggag cggaggagg ttgaatgtg gcgtgtcat 
      ttgctgctg gatacggat gtggtggtt ttggaccag 
      ccgaccaga gggatggat attttcgat acgttcttc 
      ctggtggag tatttgagg cagtgggct aatgggggg 
      gctgggaat gcattgggg aggattgtt attctgacg 
      ctgcatccg cccacctat gagattttt acgatgctg 
      tcgaggatt ttgctgatt tcggccggg aggacgatg 
      tttagtggg aggaggagg gacatgttg ccatatttc 
      gcgctagtg gattatccc tgtccctcg ttcaagaac 
      ccatcagac tattatctc gatctagta acccttgac 
      gacctctcg gccgaagcg atgctagag tcatcccaa 
      agaatcgaa caactagct gaggtcttc cgccaaaag 
      caggaacca atgagcgac ccaggccca ccctcaacc 
      ctcccaatg accgtccga aactcaaat tttctagta 
      caaggtttc gttttgttt actaagtca atgatttac 
      acccaacct gcaaccttc atcacttgg ctgaccctt 
      atcgttatt tcggccagt ctctcgctc attattggt 
      gcaatattc tgggacgta cccaacacg gacccacag 
      ctgaattta aacgacaga cttgggtac cattacagc 
      gtgatgtgt gtcatgtct tggccactt ttattactt 
      ctgactttg agtgaagtc cagaggaat aaaaacaca 
      gttgagagg gacatcaag gacgggttg tacaccaga 
      ctgacttat atcgtttca aaaagcatc ataaatctg 
      ccaccatct ctcttcatc tggttgatt tatttggtc 
      ccgagctac tccatgtcg gggctttac atgcaaagt 
      ctgaacgac tacgaaggc ttttacgtc tacattggc 
      ctcatgctg ctttaccta atcacaatt caagaattt 
      ctactcgct tttatctat tttattcca cttcgggac 
      accgcagct acgatttcc gctcttttc ctcaccgct 
      ttcttcctc tcctccgga tacctagtc cacttcaaa 
      gacttacca ccctacgta aaatttttg caatttttg 
      agtccccca acctggaca atgccatac cttctaagt 
      cgcgagtta tcccccgaa gctatagcc agtagttcg 
      gctctgatg tactgcagg aacaagcaa gtgcaacac 
      caggatatc atagtgcag ttgccttgt ccgaccgga 
      aacgggacg caagtcttg acgaattat ggatattta 
      acgtcgagt ttgaaattc tacaattat gggaatccc 
      ccgattgcg ctggtggtt ttctacgtg atttgtttc 
      gttgttgca tgttttgct tttgtttgt cgacggacg 
      ggctcgcag agacgggcc agaaacgat gccaataaa 
      ccatag
    mRNA sequence
    • >TC011713 RA
      ccagtccgt tgtgttggc aacgaattc tttgacgtc 
      aggaaagcc agtcgaacg gcattcagt ccgcttcag 
      tggctttgg tgtaacagt gaagttcat tcagttcaa 
      atgtgcgtt tttgggaaa aattcgggg aaaacggcg 
      ccaaaaaca tgaccgaat aactcgaat ataagccaa 
      gatggtgcg aagtcgcac cccttcgca ggggggtgg 
      ctcgcaagg cttcgaaat ggaacgaaa gtactcggt 
      accatcgaa cccagattc gagagcttt ctcaggcac 
      caccagcga ggacctcca cgcctggtc catctacag 
      acaaaacct caactcgga tttcacgga cagtgcctt 
      aggctccac cgacaaaag ccccctgcc ttatggcaa 
      cttccagct gcgcgacac cacggtcca gtccatatt 
      atcccaccc tcgatacgg cccaaaatc ggctttggg 
      ctcgaacat gtacacgta tttaaagtt cgggctccc 
      tcgtgtctt cccgccgaa ccacaacgg ctccaaccg 
      gtcggggac tccccagca tttcgtccg aaatgcctc 
      gacacgtcc ccacactag gggtccaaa atctcgcgg 
      tttgcgaga cggaagttc cggttacga cagttcgga 
      taatgagac gtccaatta caaacataa ccggaaata 
      ccgctcgga tccagactt ccggatgca gaacattca 
      ctatcacgg gggaagtcc cggaattcc tctcgcggc 
      aatgcaaca aggcggggt ccggactca caatcaatg 
      gaaccggaa taaaagcgt ttctgaggc caatttact 
      ggcggctga gtacgggcc gcacccgca ccataggag 
      gaacagtgt ggcggagtt tgggcacca ccatgatgt 
      ggtggacag tcacattgg ccgtcctgt gtcgaaggc 
      cgagagcca catctcaat tcccgtctc ccggagagg 
      tccctcggt catccgaac ggactactt gtcccagga 
      cgaggaccc cggaacctc cttcatcta cccgcacct 
      ccaggtcgg tggcattgg agtttggcc gaaaaccag 
      ctcctgctt cgggtccaa gcaccacct gctgctcaa 
      cgacgtctc gttcgagat ccgtggagg cgagatcat 
      ggccatcat ggccacgtc ggaggaaga gggcacagc 
      tctgctgga cctggtggc aggacacgc caagcccgt 
      cgtgggcga catcatcct caacggcca gaccgtcaa 
      atcggagat gttgaagaa ccgggtcgc gtacgtcca 
      aagtgactc gcatttgtg caaagacat gaccgttat 
      tcagacttt gcggtttca ctacgatct caaaaaacc 
      caccgataa gttgggcta tctcaagat cgatgccat 
      ggaccggat caacgtgct gatcgagga tctgggctt 
      ggagcaggt gaggaacac caaagtggc gtcgatgac 
      gatttcgga gcggaggag gttgaatgt ggcgtgtca 
      tttgctgct ggatacgga tgtggtggt tttggacca 
      gccgaccag agggatgga tattttcga tacgttctt 
      cctggtgga gtatttgag gcagtgggc taatggggg 
      ggctgggaa tgcattggg gaggattgt tattctgac 
      gctgcatcc gcccaccta tgagatttt tacgatgct 
      gtcgaggat tttgctgat ttcggccgg gaggacgat 
      gtttagtgg gaggaggag ggacatgtt gccatattt 
      cgcgctagt ggattatcc ctgtccctc gttcaagaa 
      cccatcaga ctattatct cgatctagt aacccttga 
      cgacctctc ggccgaagc gatgctaga gtcatccca 
      aagaatcga acaactagc tgaggtctt ccgccaaaa 
      gcaggaacc aatgagcga cccaggccc accctcaac 
      cctcccaat gaccgtccg aaactcaaa ttttctagt 
      acaaggttt cgttttgtt tactaagtc aatgattta 
      cacccaacc tgcaacctt catcacttg gctgaccct 
      tatcgttat ttcggccag tctctcgct cattattgg 
      tgcaatatt ctgggacgt acccaacac ggacccaca 
      gctgaattt aaacgacag acttgggta ccattacag 
      cgtgatgtg tgtcatgtc ttggccact tttattact 
      tctgacttt gagtgaagt ccagaggaa taaaaacac 
      agttgagag ggacatcaa ggacgggtt gtacaccag 
      actgactta tatcgtttc aaaaagcat cataaatct 
      gccaccatc tctcttcat ctggttgat ttatttggt 
      cccgagcta ctccatgtc ggggcttta catgcaaag 
      tctgaacga ctacgaagg cttttacgt ctacattgg 
      cctcatgct gctttacct aatcacaat tcaagaatt 
      tctactcgc ttttatcta ttttattcc acttcggga 
      caccgcagc tacgatttc cgctctttt cctcaccgc 
      tttcttcct ctcctccgg atacctagt ccacttcaa 
      agacttacc accctacgt aaaattttt gcaattttt 
      gagtccccc aacctggac aatgccata ccttctaag 
      tcgcgagtt atcccccga agctatagc cagtagttc 
      ggctctgat gtactgcag gaacaagca agtgcaaca 
      ccaggatat catagtgca gttgccttg tccgaccgg 
      aaacgggac gcaagtctt gacgaatta tggatattt 
      aacgtcgag tttgaaatt ctacaatta tgggaatcc 
      cccgattgc gctggtggt tttctacgt gatttgttt 
      cgttgttgc atgttttgc ttttgtttg tcgacggac 
      gggctcgca gagacgggc cagaaacga tgccaataa 
      accataggt tttattatt gttattatt tatcgatag 
      gtgaataaa ttttttaaa cattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY