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Gene information
General information
TC011702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14856889..14858487
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011702
    iBeetle screen iB_05085

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011702 RA
      MESSTKAWS QAGKNTQLS FYSGLGLAI LSSVFIGSS 
      FIIKKLSLL RLSRKGALR AGAGGFGYL KDWMWWLGF 
      LTMGIGELA NFAAYTVAP ASLVTPLGA LSVLVSAVL 
      ASKFLKETL NTLGKLGCL LCILGSIVL IIHSPKEQE 
      VASVAELVS KLHNTYFLN YIITVVSIT IIIIFYVGP 
      RYGSRHVMV YITLCSSVG SLTVMACKG LGLSISEIV 
      SKPSDLSYW SSSLFFLTV AVCIFIQMN YLNKALDLF 
      NTSVVTPVY YVMFTSLVI VASAILFNE WGNMTFEDI 
      LGSICGFLT VIVAIFMLQ GYRKDNYQK QSLLRSDYT 
      SVFYT
    CDS sequence
    • >TC011702 RA
      atggaatcg tcaacaaaa gcctggtct caggctggg 
      aaaaacacc caattgtcg ttttatagt ggtttgggt 
      ctcgcgatc ctgtcgtct gtttttata gggtcgagt 
      tttatcata aaaaagcta tcattgttg aggttaagt 
      cggaagggg gctttgcgg gccggagct ggggggttc 
      ggctatttg aaggactgg atgtggtgg ctggggttc 
      ctaacaatg gggatagga gagttggcg aatttcgcg 
      gcttatacg gtggcacct gcgtcgctt gtgacacct 
      ctgggggcc ctcagtgtg ctggtttcg gcagtctta 
      gcctccaag ttcctcaaa gagactcta aataccctc 
      gggaagttg ggctgtctt ttgtgcatc cttggctca 
      atcgtcctg attatccac tcgccgaag gaacaggaa 
      gtggcctcc gtcgctgag ctggtgtcc aagctccac 
      aacacctat ttccttaat tatataata actgtagtt 
      tcaataaca ataattata attttttac gtgggccct 
      cgatacggc tcgcgccat gtaatggtt tacataacg 
      ctttgttcc tcagtcggg agcttaact gtcatggcc 
      tgcaaaggc ctgggcttg tcaataagc gaaattgtg 
      tcaaagccg agcgattta agttactgg tcttccagt 
      ttatttttc ttgactgta gccgtctgt attttcatc 
      caaatgaat tatttaaac aaggctttg gatttattc 
      aacaccagt gttgtcaca ccggtatat tatgtaatg 
      tttacaagt ttagtgatt gtagcttca gcgatttta 
      ttcaacgag tggggcaat atgactttt gaggatatt 
      ctagggagt atttgcgga tttttaact gtgattgtt 
      gcgattttt atgctccaa ggctatagg aaagacaac 
      tatcagaag cagagtttg ttaaggagt gattatacg 
      agcgttttt tacacgtag
    mRNA sequence
    • >TC011702 RA
      aaaagcttc taccgtgac ttgtgcccc aaaaactgt 
      tttaagttg ttattgttt aagtttttg tacggtaaa 
      taaatgttt attttttgg aattacaag gataattaa 
      ttacataac cttactttc cctatgacg tcacaaatg 
      actctccac aaaaaccga taaaattcc cgttaataa 
      ttgatagcg catcgttaa caggtgact cagcgtgat 
      aacattatc acgaattca gtttttttc caataactc 
      gccaaacac ctagttata acctcaaaa aatggaatc 
      gtcaacaaa agcctggtc tcaggctgg gaaaaacac 
      ccaattgtc gttttatag tggtttggg tctcgcgat 
      cctgtcgtc tgtttttat agggtcgag ttttatcat 
      aaaaaagct atcattgtt gaggttaag tcggaaggg 
      ggctttgcg ggccggagc tggggggtt cggctattt 
      gaaggactg gatgtggtg gctggggtt cctaacaat 
      ggggatagg agagttggc gaatttcgc ggcttatac 
      ggtggcacc tgcgtcgct tgtgacacc tctgggggc 
      cctcagtgt gctggtttc ggcagtctt agcctccaa 
      gttcctcaa agagactct aaataccct cgggaagtt 
      gggctgtct tttgtgcat ccttggctc aatcgtcct 
      gattatcca ctcgccgaa ggaacagga agtggcctc 
      cgtcgctga gctggtgtc caagctcca caacaccta 
      tttccttaa ttatataat aactgtagt ttcaataac 
      aataattat aatttttta cgtgggccc tcgatacgg 
      ctcgcgcca tgtaatggt ttacataac gctttgttc 
      ctcagtcgg gagcttaac tgtcatggc ctgcaaagg 
      cctgggctt gtcaataag cgaaattgt gtcaaagcc 
      gagcgattt aagttactg gtcttccag tttattttt 
      cttgactgt agccgtctg tattttcat ccaaatgaa 
      ttatttaaa caaggcttt ggatttatt caacaccag 
      tgttgtcac accggtata ttatgtaat gtttacaag 
      tttagtgat tgtagcttc agcgatttt attcaacga 
      gtggggcaa tatgacttt tgaggatat tctagggag 
      tatttgcgg atttttaac tgtgattgt tgcgatttt 
      tatgctcca aggctatag gaaagacaa ctatcagaa 
      gcagagttt gttaaggag tgattatac gagcgtttt 
      ttacacgta ggtctgtat catatctac ctcaaatgt 
      gctgtgact tgttttatt tgccataat ttatttatc 
      gtgtttagg ggtttgtga cttgttagg gacatttat 
      atagttatt gccaacctt aggcaagtc gcaaacaaa 
      tgcaattat gtaaatagg tccactgta accgacaaa 
      agattttgt aaataaata tgattcagt aga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY