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Gene information
General information
TC011697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14926847..14939970
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011697
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05083

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
    tropharium shortened - Number of animals showing the phenotype: 1

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    leg musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (22 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 10

    maxillary palp elongated - Number of animals showing the phenotype: 10

    labial palp swollen - Number of animals showing the phenotype: 10

    labrum shortened - Number of animals showing the phenotype: 10
    ommatidia number decreased - Number of animals showing the phenotype: 10

    legs shortened - Number of animals showing the phenotype: 10

    female genital lobes rounded - Number of animals showing the phenotype: 10

    Adult morphology
    maxillary palp slightlysize elongated - Number of animals showing the phenotype: 10


    funicle not present - Number of animals showing the phenotype: 10


    legs swollen - Number of animals showing the phenotype: 10


    legs tarsus not present - Number of animals showing the phenotype: 10

    Comment: Pretarsus present!!! Most distal part of appendages present!!!

    legs shortened - Number of animals showing the phenotype: 10


    legs tibia proximal division boundary not present - Number of animals showing the phenotype: 10


    legs tibia potentially fused - Number of animals showing the phenotype: 10

    hindwing necrotic - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011697 RA
      MLTDMTPTA GQLYGAQIP AMSMNVKAA EHPSLRGTP 
      LAMLAAQCN KLSNKSPPP LADAAVGKG FHPWKKSPQ 
      GAPSPSSTP SSLPTQRTS TSNPTYSSR SVMTSCSSV 
      PTTASYGSD LYFPGATSQ PPTDNSHVH HHQTSLLGK 
      VEGAATHHL GSVYSRHPY ESWPFNTMS GATHHGGIK 
      SDSVTSNAW WDVHSTGSW LDMSGGMHQ MANYSTDYS 
      SLTHSLAAS NHLLSSGQH LLQDTYKSM LPGAQTVGA 
      SFGLHATGS APSPTAGAG GLPPQVPSP RSQRRYTGR 
      ATCDCPNCQ EAERLGPAG VHLRKKNIH SCHIPGCGK 
      VYGKTSHLK AHLRWHTGE RPFVCNWLF CGKRFTRSD 
      ELQRHLRTH TGEKRFACP ICNKRFMRS DHLAKHVKT 
      HNGNGKKGS SDSCSDSEE NSQSDITNN VNSPASMNQ 
      TPPPTAQMG LDMVTGLDV KPPGLV
    CDS sequence
    • >TC011697 RA
      atgcttact gatatgacc cccacggcg ggacaactc 
      tacggggcg cagattccc gccatgtcg atgaatgtc 
      aaagcggct gagcacccc agcctccgc ggcacccct 
      ctggcgatg ctcgccgcc cagtgcaac aaactctcc 
      aacaaatcg ccgcctcct ttagcggac gccgccgtc 
      ggcaagggc ttccatccg tggaaaaaa agccctcaa 
      ggtgctcca tcaccatcg tccactccc agttcgctc 
      ccaactcaa cgcacctcc acctccaac cccacctac 
      tcgagccga tcggtgatg acgtcgtgt tccagcgtc 
      ccgacgacg gcatcgtac ggcagcgat ttgtacttc 
      cctggcgcc acttcgcaa cccccaacc gacaactcc 
      cacgtccac caccaccag acctcgctg ctgggcaaa 
      gtggagggc gccgccacc catcatctg ggctcggtt 
      tactcgcga cacccgtac gagtcgtgg ccgtttaat 
      accatgtct ggggccacc catcatggg gggattaag 
      agtgattca gtgacgtcg aacgcctgg tgggacgtt 
      cactccacg gggagttgg ctggatatg agtggaggg 
      atgcaccaa atggccaac tactccact gattattcg 
      agtttaacg cattctttg gccgcttcc aaccatttg 
      ttatcgtcc ggacagcac ctcctccag gatacgtac 
      aaaagtatg cttcctggg gcgcaaacg gttggtgct 
      tcgttcgga ttacatgcc accgggtcg gcaccatcg 
      ccgacggcc ggggctgga ggtttgccg cctcaggtg 
      ccgagcccc aggtcacag agacgttac acaggccgg 
      gcaacttgt gattgcccc aattgccaa gaagccgag 
      cgtttggga cctgcagga gtgcactta aggaagaag 
      aacatccac agttgccac attccgggc tgtgggaag 
      gtttacggg aaaacgtcc catttgaaa gcccatttg 
      aggtggcat acgggtgaa agaccgttc gtttgcaac 
      tggttgttt tgtggtaaa agattcacc cgttccgat 
      gagttgcaa cgacatttg aggacccat acgggggaa 
      aaacgcttc gcttgtccc atttgcaat aagcgattt 
      atgcgttcg gaccatttg gccaaacac gtcaagacc 
      cataacggt aacgggaag aagggcagt tcggattct 
      tgcagcgat tcggaggag aatagtcaa agtgatatc 
      acaaataac gtgaactcc ccggcgtcc atgaaccag 
      acgccgccg ccaacggcc caaatgggg ctggatatg 
      gtgacggga ctcgatgtg aagcccccc ggcttggtt 
      tga
    mRNA sequence
    • >TC011697 RA
      ttgtaattt gtttcgctc atgcgcact cccaccagt 
      ccataaaaa ccggtgggc gtggcgtgc gataaatgg 
      cagcgacgt cgattggct ctcccaccg acgtcacgc 
      ccatttatg accaaacag aaggggaac ctgtgattt 
      ggacttcgt ggatccttc cccttccga gaatcagta 
      agcaatgtg atctcaaca atagcagtt gaacatttt 
      tagtgttgc gtattgttt tttctatct atacgggac 
      tgtggtttg attaaaaat acagtggcg tacgtcaac 
      atcatgctt actgatatg acccccacg gcgggacaa 
      ctctacggg gcgcagatt cccgccatg tcgatgaat 
      gtcaaagcg gctgagcac cccagcctc cgcggcacc 
      cctctggcg atgctcgcc gcccagtgc aacaaactc 
      tccaacaaa tcgccgcct cctttagcg gacgccgcc 
      gtcggcaag ggcttccat ccgtggaaa aaaagccct 
      caaggtgct ccatcacca tcgtccact cccagttcg 
      ctcccaact caacgcacc tccacctcc aaccccacc 
      tactcgagc cgatcggtg atgacgtcg tgttccagc 
      gtcccgacg acggcatcg tacggcagc gatttgtac 
      ttccctggc gccacttcg caaccccca accgacaac 
      tcccacgtc caccaccac cagacctcg ctgctgggc 
      aaagtggag ggcgccgcc acccatcat ctgggctcg 
      gtttactcg cgacacccg tacgagtcg tggccgttt 
      aataccatg tctggggcc acccatcat ggggggatt 
      aagagtgat tcagtgacg tcgaacgcc tggtgggac 
      gttcactcc acggggagt tggctggat atgagtgga 
      gggatgcac caaatggcc aactactcc actgattat 
      tcgagttta acgcattct ttggccgct tccaaccat 
      ttgttatcg tccggacag cacctcctc caggatacg 
      tacaaaagt atgcttcct ggggcgcaa acggttggt 
      gcttcgttc ggattacat gccaccggg tcggcacca 
      tcgccgacg gccggggct ggaggtttg ccgcctcag 
      gtgccgagc cccaggtca cagagacgt tacacaggc 
      cgggcaact tgtgattgc cccaattgc caagaagcc 
      gagcgtttg ggacctgca ggagtgcac ttaaggaag 
      aagaacatc cacagttgc cacattccg ggctgtggg 
      aaggtttac gggaaaacg tcccatttg aaagcccat 
      ttgaggtgg catacgggt gaaagaccg ttcgtttgc 
      aactggttg ttttgtggt aaaagattc acccgttcc 
      gatgagttg caacgacat ttgaggacc catacgggg 
      gaaaaacgc ttcgcttgt cccatttgc aataagcga 
      tttatgcgt tcggaccat ttggccaaa cacgtcaag 
      acccataac ggtaacggg aagaagggc agttcggat 
      tcttgcagc gattcggag gagaatagt caaagtgat 
      atcacaaat aacgtgaac tccccggcg tccatgaac 
      cagacgccg ccgccaacg gcccaaatg gggctggat 
      atggtgacg ggactcgat gtgaagccc cccggcttg 
      gtttgaggc cggtggggc cctacgctg ctctatcca 
      ggctatcat cgctagcgt gaggccccc atgtgataa 
      ggacttgtt tcccaaatt gtacataat gtgaatgga 
      cgctttagt tatcgtttt gctaatgga tttattgta 
      tataatata aatatttaa atattttac atgtttaag 
      tgtcggcaa ggtgaatga gaaacgtgt cagtgtttt 
      cacatatca gatacgctt tgtggtcac tttatagga 
      gctgaggtg cgaatttaa acgttttat catgtttct 
      ctttccgcc aaagaagct gtaatcctt tgtaaattt 
      tattgtaac tatatctgt aaattattc tataagagc 
      ttttgtgaa cacgttgac agtgaatat aattttcaa 
      tctaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY