Status
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Gene information
General information
TC011690 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15042360..15044267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011690
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05082

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011690 RA
      MSASLYNED SLPDLLPIY YKRLFPHTA LHKWLCYGD 
      PNVSNRREI SFTLVGDIY LRYQSFENA EDFAQALHK 
      RFPVKIDIG AIYKQRLES SGSYSSSKT PVEREIVFD 
      IDMTDYDDV RTCCTGAGV CPKCWKFMV IACKILDGA 
      LREDFGFEN ILWVFSGRR GIHCWVADK KARILDDNQ 
      RSAIAEYLT LLRGGLVVR KVKFFNMHA ATERALKVI 
      DEYFEKSIL VEQDILGTY ERLDAFLGN IDEGFSPLF 
      RDAMTKVQT SVERWKAFV NTFQTLQRE QKIPKHYRY 
      MVEEMKFYY AYPRLDINV TKSLNHLLK APFCIHPKS 
      GKVSVPFKP KFIEHFDPD AVPTVSQLL DEINTYDAK 
      TKAQEKDLA ENSQTSVKI KDYKKTSLL KYVTIFCEF 
      LRGLQASAA RNRAIKNGT VMEY
    CDS sequence
    • >TC011690 RA
      atgagtgcc tctttgtac aatgaagac agcctccct 
      gacctgctc cccatctac tacaagcgt ctgttcccc 
      catacagcg ctccacaaa tggctttgc tacggtgac 
      cccaacgtg tccaaccgg cgcgaaatt tcgttcact 
      ctagtcgga gacatttac ctccgttac cagagtttc 
      gaaaatgcg gaggacttt gcccaagcg ctccataaa 
      cgcttcccg gttaaaatc gatattggg gctatttac 
      aagcagcga cttgagtcg tctggaagt tactctagc 
      tccaaaaca ccagtcgag cgagagatt gtgtttgat 
      atcgatatg actgattat gatgatgtt cggacttgt 
      tgtacggga gctggggtg tgccccaaa tgctggaag 
      tttatggtg attgcgtgc aagattttg gatggggct 
      ttacgggag gacttcggt tttgagaac attttatgg 
      gtgttttcg ggtcggaga ggcatccat tgttgggtg 
      gctgataaa aaggcccgg atcttggat gataatcaa 
      cggtcggct attgctgaa tatttgact ttgttgcga 
      gggggcctt gtggtacga aaagtcaag tttttcaac 
      atgcatgcg gccactgag agggcctta aaagtcatt 
      gatgaatat ttcgaaaag tcgattctt gtcgaacag 
      gacatttta gggacttat gagcgcttg gacgctttt 
      ttgggcaac attgacgag ggttttagt cctttgttt 
      cgagacgca atgaccaaa gttcagact tcggtcgag 
      cgctggaag gcttttgtt aacactttt cagacgtta 
      caaagagag caaaaaata ccaaaacac tacagatac 
      atggttgag gagatgaaa ttttactat gcgtatccg 
      cgacttgat atcaacgtc accaaaagc ttaaatcac 
      ttgctgaaa gcgccgttt tgcattcac ccgaaaagt 
      ggcaaagtt tcggtgcct tttaagccc aaattcatc 
      gagcatttt gacccagat gccgtaccc acagtcagc 
      caactcttg gacgagata aacacctac gatgccaaa 
      accaaagcc caggagaaa gatttagcg gaaaattcc 
      caaaccagt gttaaaata aaagactac aagaagaca 
      agtttgctc aaatatgtt acaattttt tgcgagttt 
      ttaagaggc ctgcaggct tcagctgcc cgaaacagg 
      gccataaaa aatggaact gtaatggaa tattaa
    mRNA sequence
    • >TC011690 RA
      cctcacttt ttttggcgg gaaaagttg tcactgtgt 
      tttgcgtga tgagtgcct ctttgtaca atgaagaca 
      gcctccctg acctgctcc ccatctact acaagcgtc 
      tgttccccc atacagcgc tccacaaat ggctttgct 
      acggtgacc ccaacgtgt ccaaccggc gcgaaattt 
      cgttcactc tagtcggag acatttacc tccgttacc 
      agagtttcg aaaatgcgg aggactttg cccaagcgc 
      tccataaac gcttcccgg ttaaaatcg atattgggg 
      ctatttaca agcagcgac ttgagtcgt ctggaagtt 
      actctagct ccaaaacac cagtcgagc gagagattg 
      tgtttgata tcgatatga ctgattatg atgatgttc 
      ggacttgtt gtacgggag ctggggtgt gccccaaat 
      gctggaagt ttatggtga ttgcgtgca agattttgg 
      atggggctt tacgggagg acttcggtt ttgagaaca 
      ttttatggg tgttttcgg gtcggagag gcatccatt 
      gttgggtgg ctgataaaa aggcccgga tcttggatg 
      ataatcaac ggtcggcta ttgctgaat atttgactt 
      tgttgcgag ggggccttg tggtacgaa aagtcaagt 
      ttttcaaca tgcatgcgg ccactgaga gggccttaa 
      aagtcattg atgaatatt tcgaaaagt cgattcttg 
      tcgaacagg acattttag ggacttatg agcgcttgg 
      acgcttttt tgggcaaca ttgacgagg gttttagtc 
      ctttgtttc gagacgcaa tgaccaaag ttcagactt 
      cggtcgagc gctggaagg cttttgtta acacttttc 
      agacgttac aaagagagc aaaaaatac caaaacact 
      acagataca tggttgagg agatgaaat tttactatg 
      cgtatccgc gacttgata tcaacgtca ccaaaagct 
      taaatcact tgctgaaag cgccgtttt gcattcacc 
      cgaaaagtg gcaaagttt cggtgcctt ttaagccca 
      aattcatcg agcattttg acccagatg ccgtaccca 
      cagtcagcc aactcttgg acgagataa acacctacg 
      atgccaaaa ccaaagccc aggagaaag atttagcgg 
      aaaattccc aaaccagtg ttaaaataa aagactaca 
      agaagacaa gtttgctca aatatgtta caatttttt 
      gcgagtttt taagaggcc tgcaggctt cagctgccc 
      gaaacaggg ccataaaaa atggaactg taatggaat 
      attaattaa ttagtttac gttttttat tactcattg 
      ttactgatt acgaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY