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Gene information
General information
TC011685 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15103413..15104813
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011685
    iBeetle screen iB_05081

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011685 RA
      MEVAAMEAY KKDMETGGD LSSSLMKGK 
INSGAKITK LWNEARTRD GKTYYYNIM 
TKETVWQPP REGYLTIQE QRAQAEAEA 
QNQLKAVEK FKRQEALMT MQQVKEEEM 
ENEARKNRE KLKERRVVD DLPATTCGP 
LLEPGKTDP YGKWQTIQE RPVVDLQLP 
VQDYYYEEP VVQYEPEAP VKEFKEKTV 
ESLGGESSE TGFKKRKFG GAAKRNVRQ 
RLDDD
    CDS sequence
    • >TC011685 RA
      atggaagtg gcggcaatg gaagcctat aaaaaagac 
atggagaca ggtggcgac ctctcctcg tccctcatg 
aaggggaaa atcaacagt ggtgccaaa ataaccaaa 
ttgtggaat gaggcaagg actagagat ggcaaaacc 
tactattac aacattatg actaaggaa acagtttgg 
cagcctcca cgggagggt tacctcacc atccaggaa 
cagcgggcc caggcggaa gccgaggcc cagaaccag 
ttaaaagct gtcgagaag tttaaacgt caggaagcc 
ctcatgaca atgcaacaa gtcaaggag gaagaaatg 
gaaaatgaa gcccgaaaa aatcgggaa aaattaaaa 
gagcgcaga gtcgttgat gatttaccg gccaccact 
tgtgggccc cttttagag ccggggaag accgatcct 
tatggcaaa tggcaaaca atccaagaa aggcctgtt 
gtagattta caattgccg gttcaggac tattattac 
gaggaaccg gtggtgcaa tacgagccc gaagccccc 
gttaaggag tttaaagag aaaactgtg gagagttta 
gggggcgaa agtagcgag acagggttc aagaaacga 
aagtttgga ggcgctgcc aagcgcaac gtgagacaa 
cggctagac gatgattga
    mRNA sequence
    • >TC011685 RA
      tattacctt caggtgttt ttaaaacac tccgttctt 
ccattatta cagtatttg tcagatttc tagcgtaat 
tgtttattt ttgtaactg ttcattccc ttccgccgt 
tatttattt atttatttc catttgtta cattgtcaa 
cattcatag ttcctatat tgtaaatga tttgaattg 
tatttggat tgttgatta ttaaaacac tattaacaa 
gaaactttg tttgatttt tttcaaaag ggccgatta 
ttgggtctc acaaaaccg gaagtattg cgaattttg 
caaatgctg gatcaccga taacaagcc gagcgtcga 
ttttcacga aaaaggcag acgacatca ggaaaatgt 
caaaaaaag gctacgaaa tatcacaaa acaaagcaa 
tcaagtcca aagaaccac aaccaaaac tgatgccac 
catcaaact catggaagt ggcggcaat ggaagccta 
taaaaaaga catggagac aggtggcga cctctcctc 
gtccctcat gaaggggaa aatcaacag tggtgccaa 
aataaccaa attgtggaa tgaggcaag gactagaga 
tggcaaaac ctactatta caacattat gactaagga 
aacagtttg gcagcctcc acgggaggg ttacctcac 
catccagga acagcgggc ccaggcgga agccgaggc 
ccagaacca gttaaaagc tgtcgagaa gtttaaacg 
tcaggaagc cctcatgac aatgcaaca agtcaagga 
ggaagaaat ggaaaatga agcccgaaa aaatcggga 
aaaattaaa agagcgcag agtcgttga tgatttacc 
ggccaccac ttgtgggcc ccttttaga gccggggaa 
gaccgatcc ttatggcaa atggcaaac aatccaaga 
aaggcctgt tgtagattt acaattgcc ggttcagga 
ctattatta cgaggaacc ggtggtgca atacgagcc 
cgaagcccc cgttaagga gtttaaaga gaaaactgt 
ggagagttt agggggcga aagtagcga gacagggtt 
caagaaacg aaagtttgg aggcgctgc caagcgcaa 
cgtgagaca acggctaga cgatgattg attattttt 
gtatatata atgtattat gtaaaaaat aaagcgaaa 
aacttgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY