Status
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Gene information
General information
TC031250
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011681,TC011680
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15120002..15122283
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031250
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05079

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031250 RA
      MSKLEPLQT LCKHTGRVW DVSWHPKGQ TFASCGEDK 
      TIRIWSKDS DSKWSNKVI LTDGHKRTI REIAWSPCG 
      NYLASASFD TTTCIWDKK SGEFECNAT LEGHENEVK 
      SVSWSKSGR FLATCSRDK SVWIWEIAE EDEYDCAAV 
      LSAHTQDVK KVVWHPHDD ILASASYDN TVKLFKEDQ 
      SDNDWVCFA TLQGHESTV WSISWDKTG TRIVSCSDD 
      ATLKIWQKG QNTEETWKC VCTMSGYHN RTIYDVSWN 
      HSSDLIATA CGDDAIRIF KEEEGGDLE APTFSQVTC 
      IERAHAQDV NCVAWNPLL SDILVSCSD DGEIKLRLA 
      ALSRIKNYY STGPQMSVK EIVKTLQSY APLTLAESW 
      DNVGLLVDP MTDKQVKTI LLTNDLTED VVNEAKNFQ 
      AGLIISYHP NIFQGLKTV SDKTWKERI VIQCIRHEI 
      AVYSPHTSW DCVEGGVND WLASAFAFK NIAPIKPLE 
      NPRQGAGRI LTLDTPITV ETAVANVKK HVGVGHLRL 
      ALARNKGLD ARIETVGLC AGSGASVLK GLTSDLYLT 
      GEMSHHEVL DATQNGVHV ILCEHSNSE RGFLGRFQK 
      QLSDLLQNS VNIIVSSVD KDPLIVV
    CDS sequence
    • >TC031250 RA
      atgtcaaag cttgagccc cttcaaact ttatgcaag 
      cacacgggg cgcgtttgg gacgtgagt tggcacccg 
      aaggggcaa acgttcgcc tcgtgtggc gaagacaag 
      accatccga atctggtcg aaagactcg gactcgaag 
      tggtcgaat aaagtaatt ctaaccgat ggccacaaa 
      cgcacaatt cgggaaatt gcgtggtcc ccctgtggc 
      aattacctc gcctcggcc tcctttgac accactacg 
      tgcatttgg gacaagaaa tcgggcgag ttcgagtgc 
      aatgccact cttgagggg cacgaaaat gaggttaag 
      agtgtttcc tggtcgaag tcggggcga tttttggcc 
      acttgtagc cgtgacaag tcggtttgg atttgggag 
      attgccgag gaggatgag tatgattgc gcggccgtg 
      ttgagtgcg cacacacag gatgtgaag aaagtcgtg 
      tggcaccca catgacgat atcctcgcc tcggcctcc 
      tacgacaac acggttaag ttgttcaaa gaggaccag 
      tcagacaac gactgggtt tgttttgcc accttacag 
      gggcacgag tcaacggtt tggagcata agttgggac 
      aaaacgggg acaaggatt gtgtcgtgt agcgacgac 
      gccacgttg aaaatctgg caaaagggc caaaatacg 
      gaggaaacg tggaaatgt gtgtgtacc atgtcgggg 
      taccacaac agaaccatc tatgacgtg tcttggaac 
      cactcaagt gacttgatc gccaccgct tgcggtgac 
      gacgctatc agaattttt aaagaggag gaagggggc 
      gacttagag gcccccacg tttagccaa gtcacctgc 
      attgagagg gcgcacgcc caggatgtc aactgcgtc 
      gcctggaac ccccttctt agtgatatt ttagtgtcg 
      tgcagcgac gacggggaa attaaatta cggttggcg 
      gccctaagt cggattaaa aattactac agcacgggc 
      ccccaaatg tcggtcaaa gaaatcgtc aaaacttta 
      caatcctac gcccccttg acccttgcc gagagctgg 
      gacaatgtg gggctcctg gtggaccca atgaccgac 
      aaacaggtc aaaacaatt ctattaaca aacgacctc 
      actgaggat gtggtcaat gaagctaaa aacttccaa 
      gcggggctc attatctcc tatcaccct aatatcttt 
      caaggcctt aaaacagtc tctgacaag acttggaaa 
      gaacgaatc gtaatccaa tgtattaga catgaaatt 
      gcggtttac tcgccccat acgagctgg gattgtgtc 
      gaagggggc gttaatgat tggctagcc tcagcgttc 
      gcttttaaa aacattgcg ccaatcaaa ccattggaa 
      aatccgaga caaggggcg gggcgcatc ctcacacta 
      gacacgccc ataactgtg gaaaccgcg gtcgcaaat 
      gtcaaaaaa catgtcggg gtggggcat ctgcgactc 
      gcattggct aggaacaaa ggtttagat gcgaggatt 
      gaaaccgtg gggctgtgt gccggttcg ggggcttcg 
      gttttaaag gggctcacg agcgatttg tacctaacg 
      ggggaaatg tcccaccat gaagtcctg gatgctacg 
      caaaacggg gttcatgtg attctctgc gaacatagt 
      aattccgaa cggggcttt ttggggcgt ttccagaaa 
      caattgagt gatttgttg caaaattca gtcaatatt 
      attgtttca agtgtcgat aaagaccca ttaattgta 
      gtttga
    mRNA sequence
    • >TC031250 RA
      aacgaagca aaaacgtaa taaaatcac ccatttttt 
      cacgttttg tggcattgg taagcccct taagtacat 
      ttgccccct tttggcacg tcgtgcgtc ccccgagac 
      cacttttta accaccctg tcgttgttc gcgacccta 
      accccaaaa tgtcaaagc ttgagcccc ttcaaactt 
      tatgcaagc acacggggc gcgtttggg acgtgagtt 
      ggcacccga aggggcaaa cgttcgcct cgtgtggcg 
      aagacaaga ccatccgaa tctggtcga aagactcgg 
      actcgaagt ggtcgaata aagtaattc taaccgatg 
      gccacaaac gcacaattc gggaaattg cgtggtccc 
      cctgtggca attacctcg cctcggcct cctttgaca 
      ccactacgt gcatttggg acaagaaat cgggcgagt 
      tcgagtgca atgccactc ttgaggggc acgaaaatg 
      aggttaaga gtgtttcct ggtcgaagt cggggcgat 
      ttttggcca cttgtagcc gtgacaagt cggtttgga 
      tttgggaga ttgccgagg aggatgagt atgattgcg 
      cggccgtgt tgagtgcgc acacacagg atgtgaaga 
      aagtcgtgt ggcacccac atgacgata tcctcgcct 
      cggcctcct acgacaaca cggttaagt tgttcaaag 
      aggaccagt cagacaacg actgggttt gttttgcca 
      ccttacagg ggcacgagt caacggttt ggagcataa 
      gttgggaca aaacgggga caaggattg tgtcgtgta 
      gcgacgacg ccacgttga aaatctggc aaaagggcc 
      aaaatacgg aggaaacgt ggaaatgtg tgtgtacca 
      tgtcggggt accacaaca gaaccatct atgacgtgt 
      cttggaacc actcaagtg acttgatcg ccaccgctt 
      gcggtgacg acgctatca gaattttta aagaggagg 
      aagggggcg acttagagg cccccacgt ttagccaag 
      tcacctgca ttgagaggg cgcacgccc aggatgtca 
      actgcgtcg cctggaacc cccttctta gtgatattt 
      tagtgtcgt gcagcgacg acggggaaa ttaaattac 
      ggttggcgg ccctaagtc ggattaaaa attactaca 
      gcacgggcc cccaaatgt cggtcaaag aaatcgtca 
      aaactttac aatcctacg cccccttga cccttgccg 
      agagctggg acaatgtgg ggctcctgg tggacccaa 
      tgaccgaca aacaggtca aaacaattc tattaacaa 
      acgacctca ctgaggatg tggtcaatg aagctaaaa 
      acttccaag cggggctca ttatctcct atcacccta 
      atatctttc aaggcctta aaacagtct ctgacaaga 
      cttggaaag aacgaatcg taatccaat gtattagac 
      atgaaattg cggtttact cgccccata cgagctggg 
      attgtgtcg aagggggcg ttaatgatt ggctagcct 
      cagcgttcg cttttaaaa acattgcgc caatcaaac 
      cattggaaa atccgagac aaggggcgg ggcgcatcc 
      tcacactag acacgccca taactgtgg aaaccgcgg 
      tcgcaaatg tcaaaaaac atgtcgggg tggggcatc 
      tgcgactcg cattggcta ggaacaaag gtttagatg 
      cgaggattg aaaccgtgg ggctgtgtg ccggttcgg 
      gggcttcgg ttttaaagg ggctcacga gcgatttgt 
      acctaacgg gggaaatgt cccaccatg aagtcctgg 
      atgctacgc aaaacgggg ttcatgtga ttctctgcg 
      aacatagta attccgaac ggggctttt tggggcgtt 
      tccagaaac aattgagtg atttgttgc aaaattcag 
      tcaatatta ttgtttcaa gtgtcgata aagacccat 
      taattgtag tttgagtag gctggtgaa taaacaatc 
      gtgtcaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY