General information | |||
TC031250
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15120002..15122283 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC031250 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05079 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
- >TC031250 RA
MSKLEPLQT LCKHTGRVW DVSWHPKGQ TFASCGEDK TIRIWSKDS DSKWSNKVI LTDGHKRTI REIAWSPCG NYLASASFD TTTCIWDKK SGEFECNAT LEGHENEVK SVSWSKSGR FLATCSRDK SVWIWEIAE EDEYDCAAV LSAHTQDVK KVVWHPHDD ILASASYDN TVKLFKEDQ SDNDWVCFA TLQGHESTV WSISWDKTG TRIVSCSDD ATLKIWQKG QNTEETWKC VCTMSGYHN RTIYDVSWN HSSDLIATA CGDDAIRIF KEEEGGDLE APTFSQVTC IERAHAQDV NCVAWNPLL SDILVSCSD DGEIKLRLA ALSRIKNYY STGPQMSVK EIVKTLQSY APLTLAESW DNVGLLVDP MTDKQVKTI LLTNDLTED VVNEAKNFQ AGLIISYHP NIFQGLKTV SDKTWKERI VIQCIRHEI AVYSPHTSW DCVEGGVND WLASAFAFK NIAPIKPLE NPRQGAGRI LTLDTPITV ETAVANVKK HVGVGHLRL ALARNKGLD ARIETVGLC AGSGASVLK GLTSDLYLT GEMSHHEVL DATQNGVHV ILCEHSNSE RGFLGRFQK QLSDLLQNS VNIIVSSVD KDPLIVV
- >TC031250 RA
atgtcaaag cttgagccc cttcaaact ttatgcaag cacacgggg cgcgtttgg gacgtgagt tggcacccg aaggggcaa acgttcgcc tcgtgtggc gaagacaag accatccga atctggtcg aaagactcg gactcgaag tggtcgaat aaagtaatt ctaaccgat ggccacaaa cgcacaatt cgggaaatt gcgtggtcc ccctgtggc aattacctc gcctcggcc tcctttgac accactacg tgcatttgg gacaagaaa tcgggcgag ttcgagtgc aatgccact cttgagggg cacgaaaat gaggttaag agtgtttcc tggtcgaag tcggggcga tttttggcc acttgtagc cgtgacaag tcggtttgg atttgggag attgccgag gaggatgag tatgattgc gcggccgtg ttgagtgcg cacacacag gatgtgaag aaagtcgtg tggcaccca catgacgat atcctcgcc tcggcctcc tacgacaac acggttaag ttgttcaaa gaggaccag tcagacaac gactgggtt tgttttgcc accttacag gggcacgag tcaacggtt tggagcata agttgggac aaaacgggg acaaggatt gtgtcgtgt agcgacgac gccacgttg aaaatctgg caaaagggc caaaatacg gaggaaacg tggaaatgt gtgtgtacc atgtcgggg taccacaac agaaccatc tatgacgtg tcttggaac cactcaagt gacttgatc gccaccgct tgcggtgac gacgctatc agaattttt aaagaggag gaagggggc gacttagag gcccccacg tttagccaa gtcacctgc attgagagg gcgcacgcc caggatgtc aactgcgtc gcctggaac ccccttctt agtgatatt ttagtgtcg tgcagcgac gacggggaa attaaatta cggttggcg gccctaagt cggattaaa aattactac agcacgggc ccccaaatg tcggtcaaa gaaatcgtc aaaacttta caatcctac gcccccttg acccttgcc gagagctgg gacaatgtg gggctcctg gtggaccca atgaccgac aaacaggtc aaaacaatt ctattaaca aacgacctc actgaggat gtggtcaat gaagctaaa aacttccaa gcggggctc attatctcc tatcaccct aatatcttt caaggcctt aaaacagtc tctgacaag acttggaaa gaacgaatc gtaatccaa tgtattaga catgaaatt gcggtttac tcgccccat acgagctgg gattgtgtc gaagggggc gttaatgat tggctagcc tcagcgttc gcttttaaa aacattgcg ccaatcaaa ccattggaa aatccgaga caaggggcg gggcgcatc ctcacacta gacacgccc ataactgtg gaaaccgcg gtcgcaaat gtcaaaaaa catgtcggg gtggggcat ctgcgactc gcattggct aggaacaaa ggtttagat gcgaggatt gaaaccgtg gggctgtgt gccggttcg ggggcttcg gttttaaag gggctcacg agcgatttg tacctaacg ggggaaatg tcccaccat gaagtcctg gatgctacg caaaacggg gttcatgtg attctctgc gaacatagt aattccgaa cggggcttt ttggggcgt ttccagaaa caattgagt gatttgttg caaaattca gtcaatatt attgtttca agtgtcgat aaagaccca ttaattgta gtttga
- >TC031250 RA
aacgaagca aaaacgtaa taaaatcac ccatttttt cacgttttg tggcattgg taagcccct taagtacat ttgccccct tttggcacg tcgtgcgtc ccccgagac cacttttta accaccctg tcgttgttc gcgacccta accccaaaa tgtcaaagc ttgagcccc ttcaaactt tatgcaagc acacggggc gcgtttggg acgtgagtt ggcacccga aggggcaaa cgttcgcct cgtgtggcg aagacaaga ccatccgaa tctggtcga aagactcgg actcgaagt ggtcgaata aagtaattc taaccgatg gccacaaac gcacaattc gggaaattg cgtggtccc cctgtggca attacctcg cctcggcct cctttgaca ccactacgt gcatttggg acaagaaat cgggcgagt tcgagtgca atgccactc ttgaggggc acgaaaatg aggttaaga gtgtttcct ggtcgaagt cggggcgat ttttggcca cttgtagcc gtgacaagt cggtttgga tttgggaga ttgccgagg aggatgagt atgattgcg cggccgtgt tgagtgcgc acacacagg atgtgaaga aagtcgtgt ggcacccac atgacgata tcctcgcct cggcctcct acgacaaca cggttaagt tgttcaaag aggaccagt cagacaacg actgggttt gttttgcca ccttacagg ggcacgagt caacggttt ggagcataa gttgggaca aaacgggga caaggattg tgtcgtgta gcgacgacg ccacgttga aaatctggc aaaagggcc aaaatacgg aggaaacgt ggaaatgtg tgtgtacca tgtcggggt accacaaca gaaccatct atgacgtgt cttggaacc actcaagtg acttgatcg ccaccgctt gcggtgacg acgctatca gaattttta aagaggagg aagggggcg acttagagg cccccacgt ttagccaag tcacctgca ttgagaggg cgcacgccc aggatgtca actgcgtcg cctggaacc cccttctta gtgatattt tagtgtcgt gcagcgacg acggggaaa ttaaattac ggttggcgg ccctaagtc ggattaaaa attactaca gcacgggcc cccaaatgt cggtcaaag aaatcgtca aaactttac aatcctacg cccccttga cccttgccg agagctggg acaatgtgg ggctcctgg tggacccaa tgaccgaca aacaggtca aaacaattc tattaacaa acgacctca ctgaggatg tggtcaatg aagctaaaa acttccaag cggggctca ttatctcct atcacccta atatctttc aaggcctta aaacagtct ctgacaaga cttggaaag aacgaatcg taatccaat gtattagac atgaaattg cggtttact cgccccata cgagctggg attgtgtcg aagggggcg ttaatgatt ggctagcct cagcgttcg cttttaaaa acattgcgc caatcaaac cattggaaa atccgagac aaggggcgg ggcgcatcc tcacactag acacgccca taactgtgg aaaccgcgg tcgcaaatg tcaaaaaac atgtcgggg tggggcatc tgcgactcg cattggcta ggaacaaag gtttagatg cgaggattg aaaccgtgg ggctgtgtg ccggttcgg gggcttcgg ttttaaagg ggctcacga gcgatttgt acctaacgg gggaaatgt cccaccatg aagtcctgg atgctacgc aaaacgggg ttcatgtga ttctctgcg aacatagta attccgaac ggggctttt tggggcgtt tccagaaac aattgagtg atttgttgc aaaattcag tcaatatta ttgtttcaa gtgtcgata aagacccat taattgtag tttgagtag gctggtgaa taaacaatc gtgtcaaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
