Status
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Gene information
General information
TC011676 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15133992..15138433
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011676
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05078

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011676 RA
      MFKLFVLAL CATATLATV EKAIVCLKS 
      GDIDGKITF TQTAEGVQV EGVINGLPK 
      GKHGFHIHE KGALGDSCK DAGGHFNPD 
      KKDHGAPED AVRHVGDLG NIIADDKKV 
      AHVNISDKI ISLNGEHSI IGRAVVVHE 
      GEDDLGKGN FNDSKTTGH AGARLVCGV 
      IGIASDGTE TCPEGPGNI AISTTGNYF 
      LLFTGLYTL FLNLRF
    • >TC011676 RB
      MFKLFVLAL CATATLATV EKAIVCLKS 
      GDIDGKITF TQTAEGVQV EGVINGLPK 
      GKHGFHIHE KGALGDSCK DAGGHFNPD 
      KKDHGAPED AVRHVGDLG NIIADDKKV 
      AHVNISDKI ISLNGEHSI IGRAVVVHE 
      GEDDLGKGN FNDSKTTGH AGARLVCGV 
      IGIA
    CDS sequence
    • >TC011676 RA
      atgttcaaa cttttcgtc ctggcactt tgcgccacc 
      gccacactg gccacagtg gagaaagcc atcgtgtgc 
      ctcaaatcg ggcgacatc gacggcaaa atcaccttc 
      acccaaacc gcagaaggc gtccaagtc gaaggcgtg 
      atcaacggg ctcccgaag ggcaagcac ggcttccac 
      atccacgag aagggggcc cttggggac agctgcaag 
      gacgcaggg ggccacttt aacccggac aaaaaagat 
      cacggggct cctgaggat gctgttcgc cacgttggt 
      gatctcggg aacatcatc gctgatgat aagaaagtg 
      gcccatgtt aacatttcc gacaagatc atttcgctg 
      aatggtgaa cacagtatt atagggagg gctgtggtg 
      gtccatgag ggggaggac gatctgggg aaagggaac 
      ttcaatgac tccaaaacc acaggacat gctggggcc 
      agacttgtc tgcggcgtc atcggaatt gcaagtgat 
      gggactgag acatgtccg gaaggacct ggaaacatt 
      gccatttct acgaccgga aattacttc ctgttattt 
      actggactg tatacacta tttcttaat ctaagattt 
      taa
    • >TC011676 RB
      atgttcaaa cttttcgtc ctggcactt tgcgccacc 
      gccacactg gccacagtg gagaaagcc atcgtgtgc 
      ctcaaatcg ggcgacatc gacggcaaa atcaccttc 
      acccaaacc gcagaaggc gtccaagtc gaaggcgtg 
      atcaacggg ctcccgaag ggcaagcac ggcttccac 
      atccacgag aagggggcc cttggggac agctgcaag 
      gacgcaggg ggccacttt aacccggac aaaaaagat 
      cacggggct cctgaggat gctgttcgc cacgttggt 
      gatctcggg aacatcatc gctgatgat aagaaagtg 
      gcccatgtt aacatttcc gacaagatc atttcgctg 
      aatggtgaa cacagtatt atagggagg gctgtggtg 
      gtccatgag ggggaggac gatctgggg aaagggaac 
      ttcaatgac tccaaaacc acaggacat gctggggcc 
      agacttgtc tgcggcgtc atcggaatt gcgtaa
    mRNA sequence
    • >TC011676 RA
      aattaatgg acaccgttt tcgacgtaa ttaacgtgt 
      aaacagttc aagtgttgt gataaagga aaagaatag 
      atagcgggg taaaagcaa gtcagttcg agtcgaaaa 
      gagctcagt ggaatagtg ttatcatta atcaaagtg 
      acagtgaag ctgtgttga aaaaatatt caggtgtgg 
      taaagcagc actcaaaat gttcaaact tttcgtcct 
      ggcactttg cgccaccgc cacactggc cacagtgga 
      gaaagccat cgtgtgcct caaatcggg cgacatcga 
      cggcaaaat caccttcac ccaaaccgc agaaggcgt 
      ccaagtcga aggcgtgat caacgggct cccgaaggg 
      caagcacgg cttccacat ccacgagaa gggggccct 
      tggggacag ctgcaagga cgcaggggg ccactttaa 
      cccggacaa aaaagatca cggggctcc tgaggatgc 
      tgttcgcca cgttggtga tctcgggaa catcatcgc 
      tgatgataa gaaagtggc ccatgttaa catttccga 
      caagatcat ttcgctgaa tggtgaaca cagtattat 
      agggagggc tgtggtggt ccatgaggg ggaggacga 
      tctggggaa agggaactt caatgactc caaaaccac 
      aggacatgc tggggccag acttgtctg cggcgtcat 
      cggaattgc aagtgatgg gactgagac atgtccgga 
      aggacctgg aaacattgc catttctac gaccggaaa 
      ttacttcct gttatttac tggactgta tacactatt 
      tcttaatct aagatttta aactttcga agacgaaat 
      aaaattgta atatttttt gttgtaata aattttaac 
      acaagaa
    • >TC011676 RB
      taatcccgc gtccatctc attgtccat tatgcaaaa 
      aaccggtta caacaattt acccaaggt attgcgttg 
      tttatcaaa cgtagcatt cgttatccc aacgcagtc 
      cactttcta ccgttggac ttggcatac atcagcgag 
      aaatttatt tctaggtgt ggtaaagca gcactcaaa 
      atgttcaaa cttttcgtc ctggcactt tgcgccacc 
      gccacactg gccacagtg gagaaagcc atcgtgtgc 
      ctcaaatcg ggcgacatc gacggcaaa atcaccttc 
      acccaaacc gcagaaggc gtccaagtc gaaggcgtg 
      atcaacggg ctcccgaag ggcaagcac ggcttccac 
      atccacgag aagggggcc cttggggac agctgcaag 
      gacgcaggg ggccacttt aacccggac aaaaaagat 
      cacggggct cctgaggat gctgttcgc cacgttggt 
      gatctcggg aacatcatc gctgatgat aagaaagtg 
      gcccatgtt aacatttcc gacaagatc atttcgctg 
      aatggtgaa cacagtatt atagggagg gctgtggtg 
      gtccatgag ggggaggac gatctgggg aaagggaac 
      ttcaatgac tccaaaacc acaggacat gctggggcc 
      agacttgtc tgcggcgtc atcggaatt gcgtaaata 
      gttgttcca agcttgtgt tttgtacct tttttgcta 
      aataaagtc gaacaaatt tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY