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Gene information
General information
TC011676 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15133992..15138433
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011676
    iBeetle screen iB_05078

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011676 RA
      MFKLFVLAL CATATLATV EKAIVCLKS 
GDIDGKITF TQTAEGVQV EGVINGLPK 
GKHGFHIHE KGALGDSCK DAGGHFNPD 
KKDHGAPED AVRHVGDLG NIIADDKKV 
AHVNISDKI ISLNGEHSI IGRAVVVHE 
GEDDLGKGN FNDSKTTGH AGARLVCGV 
IGIASDGTE TCPEGPGNI AISTTGNYF 
LLFTGLYTL FLNLRF
    • >TC011676 RB
      MFKLFVLAL CATATLATV EKAIVCLKS 
GDIDGKITF TQTAEGVQV EGVINGLPK 
GKHGFHIHE KGALGDSCK DAGGHFNPD 
KKDHGAPED AVRHVGDLG NIIADDKKV 
AHVNISDKI ISLNGEHSI IGRAVVVHE 
GEDDLGKGN FNDSKTTGH AGARLVCGV 
IGIA
    CDS sequence
    • >TC011676 RA
      atgttcaaa cttttcgtc ctggcactt tgcgccacc 
gccacactg gccacagtg gagaaagcc atcgtgtgc 
ctcaaatcg ggcgacatc gacggcaaa atcaccttc 
acccaaacc gcagaaggc gtccaagtc gaaggcgtg 
atcaacggg ctcccgaag ggcaagcac ggcttccac 
atccacgag aagggggcc cttggggac agctgcaag 
gacgcaggg ggccacttt aacccggac aaaaaagat 
cacggggct cctgaggat gctgttcgc cacgttggt 
gatctcggg aacatcatc gctgatgat aagaaagtg 
gcccatgtt aacatttcc gacaagatc atttcgctg 
aatggtgaa cacagtatt atagggagg gctgtggtg 
gtccatgag ggggaggac gatctgggg aaagggaac 
ttcaatgac tccaaaacc acaggacat gctggggcc 
agacttgtc tgcggcgtc atcggaatt gcaagtgat 
gggactgag acatgtccg gaaggacct ggaaacatt 
gccatttct acgaccgga aattacttc ctgttattt 
actggactg tatacacta tttcttaat ctaagattt 
taa
    • >TC011676 RB
      atgttcaaa cttttcgtc ctggcactt tgcgccacc 
gccacactg gccacagtg gagaaagcc atcgtgtgc 
ctcaaatcg ggcgacatc gacggcaaa atcaccttc 
acccaaacc gcagaaggc gtccaagtc gaaggcgtg 
atcaacggg ctcccgaag ggcaagcac ggcttccac 
atccacgag aagggggcc cttggggac agctgcaag 
gacgcaggg ggccacttt aacccggac aaaaaagat 
cacggggct cctgaggat gctgttcgc cacgttggt 
gatctcggg aacatcatc gctgatgat aagaaagtg 
gcccatgtt aacatttcc gacaagatc atttcgctg 
aatggtgaa cacagtatt atagggagg gctgtggtg 
gtccatgag ggggaggac gatctgggg aaagggaac 
ttcaatgac tccaaaacc acaggacat gctggggcc 
agacttgtc tgcggcgtc atcggaatt gcgtaa
    mRNA sequence
    • >TC011676 RA
      aattaatgg acaccgttt tcgacgtaa ttaacgtgt 
aaacagttc aagtgttgt gataaagga aaagaatag 
atagcgggg taaaagcaa gtcagttcg agtcgaaaa 
gagctcagt ggaatagtg ttatcatta atcaaagtg 
acagtgaag ctgtgttga aaaaatatt caggtgtgg 
taaagcagc actcaaaat gttcaaact tttcgtcct 
ggcactttg cgccaccgc cacactggc cacagtgga 
gaaagccat cgtgtgcct caaatcggg cgacatcga 
cggcaaaat caccttcac ccaaaccgc agaaggcgt 
ccaagtcga aggcgtgat caacgggct cccgaaggg 
caagcacgg cttccacat ccacgagaa gggggccct 
tggggacag ctgcaagga cgcaggggg ccactttaa 
cccggacaa aaaagatca cggggctcc tgaggatgc 
tgttcgcca cgttggtga tctcgggaa catcatcgc 
tgatgataa gaaagtggc ccatgttaa catttccga 
caagatcat ttcgctgaa tggtgaaca cagtattat 
agggagggc tgtggtggt ccatgaggg ggaggacga 
tctggggaa agggaactt caatgactc caaaaccac 
aggacatgc tggggccag acttgtctg cggcgtcat 
cggaattgc aagtgatgg gactgagac atgtccgga 
aggacctgg aaacattgc catttctac gaccggaaa 
ttacttcct gttatttac tggactgta tacactatt 
tcttaatct aagatttta aactttcga agacgaaat 
aaaattgta atatttttt gttgtaata aattttaac 
acaagaa
    • >TC011676 RB
      taatcccgc gtccatctc attgtccat tatgcaaaa 
aaccggtta caacaattt acccaaggt attgcgttg 
tttatcaaa cgtagcatt cgttatccc aacgcagtc 
cactttcta ccgttggac ttggcatac atcagcgag 
aaatttatt tctaggtgt ggtaaagca gcactcaaa 
atgttcaaa cttttcgtc ctggcactt tgcgccacc 
gccacactg gccacagtg gagaaagcc atcgtgtgc 
ctcaaatcg ggcgacatc gacggcaaa atcaccttc 
acccaaacc gcagaaggc gtccaagtc gaaggcgtg 
atcaacggg ctcccgaag ggcaagcac ggcttccac 
atccacgag aagggggcc cttggggac agctgcaag 
gacgcaggg ggccacttt aacccggac aaaaaagat 
cacggggct cctgaggat gctgttcgc cacgttggt 
gatctcggg aacatcatc gctgatgat aagaaagtg 
gcccatgtt aacatttcc gacaagatc atttcgctg 
aatggtgaa cacagtatt atagggagg gctgtggtg 
gtccatgag ggggaggac gatctgggg aaagggaac 
ttcaatgac tccaaaacc acaggacat gctggggcc 
agacttgtc tgcggcgtc atcggaatt gcgtaaata 
gttgttcca agcttgtgt tttgtacct tttttgcta 
aataaagtc gaacaaatt tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY