Status
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Gene information
General information
TC034465
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011669,TC011670
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16175687..16181374
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034465
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05075

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034465 RA
      MEFPSDLNA VLKTPNSIS SRKQVDFSR ARPTLVIPP 
      PQDQWTQYT SASSGRTPQ DYSRKQSIF YEGIDGIRD 
      FTKSGLGLG EKCAYWLYN KLKLWSQKW FTHMFLTVV 
      LIFYTIGGA LIFEVIEGR AESTVELDL KDSRDELLR 
      ELRVTSLQT PVEKSLDEW IGEASRHIQ KRYESRLEN 
      YYSHHKLAV TNGIEKKIW TFWNSVVFC STVYTSIEI 
      YDDSKREEK EVDIARIEA MVTPEQMHS ASVAKKRRN 
      FTKRRASRR DKTPLKELD DKTTTDEDV PEQRPKTPE 
      RRRRKKIVS KDESKSEPE FTEKMESAT IKKSHSFSK 
      QVIDEYKKR ETLSELAQA EVVSFFNKT KKGLTAAAI 
      RGRERFKKL DKEQVRRSQ SAPHAFTQE ELIESFRKA 
      QLLDEFNVQ LQTKIYDVY ESDNISVSK QFRPEVQEF 
      VRQRKKQRT TNCSKIVEI LDPKTSNVI AIKSVIKAD 
      FHIRQLFDI VRLFTIKEL LVYFRAFKS DMKDECRKI 
      RILRNRCMC ELFLIITFC GLGGFLFKF VEGSFEHFY 
      KCGVKRVKR DFIEMLWLK SHNLREEEW KSLARNKLR 
      TFEEELHTA HEAGMKTYS GQRSWSFLN SVVYCLTIV 
      TTIGYGHIY PETRTGKAL TIVYSLIGI PLFLLALTD 
      FGKLFTRCI KFLWSFVRR LYYTGSCRK VRKTAHVKE 
      IVKGAQMMY EIATFRRPS VFAEGEQAD TPSPTTPAM 
      SNFEIDDEF NLPVTLAIF ILVVYMFVG ALIYWLWEA 
      WNFFDSFYF VFISMSTVG FGDMVPNDA ACMMVSIVY 
      LVFGLALMS MCINVVQAK LSDTFQQAS TKIGATIGL 
      SVAEDDGSI TTAVPDNVE VVPVHDKAK ISTKDS
    CDS sequence
    • >TC034465 RA
      atggaattc ccgagtgat ttaaacgcg gttttaaaa 
      acaccaaat tcaatatca tcgcgtaaa caagtggac 
      tttagtcgt gccagacct actttagtt attcctcca 
      ccacaagat caatggact caatacaca tcagccagt 
      tccggtagg actccacaa gactattca agaaaacaa 
      tcaattttt tacgaagga atcgacgga atcagagat 
      tttacaaag tctggcttg gggcttggg gaaaaatgt 
      gcttattgg ttatacaat aaacttaaa ctttggagt 
      caaaaatgg tttacgcac atgtttttg accgttgtg 
      cttatattt tacactatt ggtggagca ttaattttc 
      gaggtaatt gaaggccga gcggaatca acggtagaa 
      ctagattta aaagattcc agagatgaa cttcttaga 
      gaacttcga gtgacttct ctccaaact cctgttgag 
      aaatctctc gacgaatgg ataggagag gcatcaaga 
      catatccaa aaacgctac gagagtaga ctagaaaat 
      tattattcg catcataag cttgccgtt actaacggt 
      attgaaaag aaaatttgg acgttttgg aattccgtc 
      gttttctgc agtactgtg tacactagt atagaaatt 
      tacgatgat tcaaaacga gaagaaaaa gaagtagat 
      atagctaga atcgaagca atggttaca cctgaacaa 
      atgcatagt gcttctgtg gcaaaaaaa cggcgaaac 
      tttacgaaa cgacgagct agccgtcga gataaaaca 
      cctcttaaa gaactcgac gataaaacg acaacagac 
      gaagatgtt ccagagcaa cgaccaaaa acaccggaa 
      agaagacgt cgaaaaaaa atcgtgagt aaagacgaa 
      tcgaaatct gaaccggag tttactgaa aaaatggaa 
      tctgctact ataaaaaaa tcacacagt ttttccaaa 
      caagtcata gatgagtac aagaaaaga gaaaccttg 
      agtgagtta gcccaggcc gaagttgtt agttttttt 
      aataaaacc aaaaaagga ctaactgca gctgcaata 
      agaggccgc gagagattt aaaaagttg gacaaggaa 
      caggtgaga agaagtcaa tcagcacct cacgcattt 
      acacaagaa gaattgatt gaaagcttt cgcaaagca 
      caacttttg gatgaattt aatgtacaa ctccaaacg 
      aaaatctat gatgtttac gaaagcgac aatatttct 
      gtctcgaaa cagtttcga ccagaggtg caagaattt 
      gtgcgccaa agaaaaaaa caacgcacc acaaattgt 
      tccaaaatt gtagagatt ttagatcca aaaacatcg 
      aatgtgatt gcaattaaa tcagtgata aaagcagat 
      tttcatata aggcaactt tttgatatt gttcgactt 
      tttacgatt aaagaattg ctggtgtat tttcgtgca 
      tttaagtcc gatatgaaa gacgaatgt cgaaagatt 
      cgtatctta agaaatcga tgtatgtgc gaacttttt 
      cttattatt actttttgt ggtctgggt ggattttta 
      tttaaattt gttgaagga agttttgag catttttac 
      aaatgcggc gttaaacga gttaaacgc gactttatc 
      gagatgtta tggcttaaa agtcataat cttagagaa 
      gaagaatgg aaaagttta gcaagaaat aaattgagg 
      acgtttgaa gaagagctc catactgca cacgaagcc 
      ggaatgaaa acatacagt ggtcaaagg tcgtggagt 
      tttctaaat tccgttgtt tattgtcta actatagtt 
      acaaccatt ggttatggt cacatttat cctgaaaca 
      agaactgga aaagctttg accatcgtc tactctctt 
      atcggtatc ccccttttt cttctggca ttaaccgat 
      ttcggcaaa ctctttaca agatgcatt aaatttctt 
      tggtcgttt gttagacga ctttattat actggaagc 
      tgtcgaaaa gtgcgaaaa acagcacac gtgaaggaa 
      attgtcaaa ggagcacaa atgatgtac gaaattgcg 
      acatttcgt cgcccttca gtgttcgcc gagggcgaa 
      caagccgac accccttca ccaacaact cctgcaatg 
      tccaacttt gaaattgac gacgaattt aatcttccc 
      gttacgtta gccattttt attcttgtc gtttatatg 
      tttgtcggc gctcttatc tattggctg tgggaggct 
      tggaacttt tttgattct ttttacttt gtttttatt 
      tcaatgtca acagttgga tttggagat atggtaccg 
      aacgacgcc gcttgtatg atggtctct attgtgtac 
      ctagttttt ggcctcgct ctaatgtcg atgtgtatc 
      aatgtggta caagcaaaa ctgagtgat acttttcag 
      caagcttct actaaaatt ggtgccact attggactt 
      tccgtcgcc gaagatgac ggcagcatc actacagcg 
      gttcccgac aacgttgaa gtggttccg gttcacgac 
      aaggccaaa atttcgact aaagacagc tga
    mRNA sequence
    • >TC034465 RA
      gcacattgt tgctattta taaacagct ggcagaaaa 
      gatgacgtt gtagaataa gcaaaaatg atttttcat 
      cttggtagg aaactagct cgtaaatat ctaaattta 
      tatcgacaa tatttaaac aacatgaac ccgtatctg 
      ttatgctgc tttgattta atgccctat aatatttta 
      atgcacata aataactcc gtgaataat aaatttaat 
      acagacaaa aattacata aaacaattt ccaggaata 
      tactgttgg gtaaaacta agtacgtta gaaatattc 
      tttctttag taatttgtg aagaattct tgacctaaa 
      ctgccctat ttttaaata attttggct gtgccacta 
      actacctta gtctcaaaa ttctgcgta aaccatcgg 
      atctcatca ctggcgtcg gaaatattt ataagacag 
      tagaaaaca aacggcgtc acgaccagc agagtgcaa 
      ctgtgccgg gatgctatg ttagtttaa cttttgatc 
      gatattgag atgtgatgg aattcccga gtgatttaa 
      acgcggttt taaaaacac caaattcaa tatcatcgc 
      gtaaacaag tggacttta gtcgtgcca gacctactt 
      tagttattc ctccaccac aagatcaat ggactcaat 
      acacatcag ccagttccg gtaggactc cacaagact 
      attcaagaa aacaatcaa ttttttacg aaggaatcg 
      acggaatca gagatttta caaagtctg gcttggggc 
      ttggggaaa aatgtgctt attggttat acaataaac 
      ttaaacttt ggagtcaaa aatggttta cgcacatgt 
      ttttgaccg ttgtgctta tattttaca ctattggtg 
      gagcattaa ttttcgagg taattgaag gccgagcgg 
      aatcaacgg tagaactag atttaaaag attccagag 
      atgaacttc ttagagaac ttcgagtga cttctctcc 
      aaactcctg ttgagaaat ctctcgacg aatggatag 
      gagaggcat caagacata tccaaaaac gctacgaga 
      gtagactag aaaattatt attcgcatc ataagcttg 
      ccgttacta acggtattg aaaagaaaa tttggacgt 
      tttggaatt ccgtcgttt tctgcagta ctgtgtaca 
      ctagtatag aaatttacg atgattcaa aacgagaag 
      aaaaagaag tagatatag ctagaatcg aagcaatgg 
      ttacacctg aacaaatgc atagtgctt ctgtggcaa 
      aaaaacggc gaaacttta cgaaacgac gagctagcc 
      gtcgagata aaacacctc ttaaagaac tcgacgata 
      aaacgacaa cagacgaag atgttccag agcaacgac 
      caaaaacac cggaaagaa gacgtcgaa aaaaaatcg 
      tgagtaaag acgaatcga aatctgaac cggagttta 
      ctgaaaaaa tggaatctg ctactataa aaaaatcac 
      acagttttt ccaaacaag tcatagatg agtacaaga 
      aaagagaaa ccttgagtg agttagccc aggccgaag 
      ttgttagtt tttttaata aaaccaaaa aaggactaa 
      ctgcagctg caataagag gccgcgaga gatttaaaa 
      agttggaca aggaacagg tgagaagaa gtcaatcag 
      cacctcacg catttacac aagaagaat tgattgaaa 
      gctttcgca aagcacaac ttttggatg aatttaatg 
      tacaactcc aaacgaaaa tctatgatg tttacgaaa 
      gcgacaata tttctgtct cgaaacagt ttcgaccag 
      aggtgcaag aatttgtgc gccaaagaa aaaaacaac 
      gcaccacaa attgttcca aaattgtag agattttag 
      atccaaaaa catcgaatg tgattgcaa ttaaatcag 
      tgataaaag cagattttc atataaggc aactttttg 
      atattgttc gacttttta cgattaaag aattgctgg 
      tgtattttc gtgcattta agtccgata tgaaagacg 
      aatgtcgaa agattcgta tcttaagaa atcgatgta 
      tgtgcgaac tttttctta ttattactt tttgtggtc 
      tgggtggat ttttattta aatttgttg aaggaagtt 
      ttgagcatt tttacaaat gcggcgtta aacgagtta 
      aacgcgact ttatcgaga tgttatggc ttaaaagtc 
      ataatctta gagaagaag aatggaaaa gtttagcaa 
      gaaataaat tgaggacgt ttgaagaag agctccata 
      ctgcacacg aagccggaa tgaaaacat acagtggtc 
      aaaggtcgt ggagttttc taaattccg ttgtttatt 
      gtctaacta tagttacaa ccattggtt atggtcaca 
      tttatcctg aaacaagaa ctggaaaag ctttgacca 
      tcgtctact ctcttatcg gtatccccc tttttcttc 
      tggcattaa ccgatttcg gcaaactct ttacaagat 
      gcattaaat ttctttggt cgtttgtta gacgacttt 
      attatactg gaagctgtc gaaaagtgc gaaaaacag 
      cacacgtga aggaaattg tcaaaggag cacaaatga 
      tgtacgaaa ttgcgacat ttcgtcgcc cttcagtgt 
      tcgccgagg gcgaacaag ccgacaccc cttcaccaa 
      caactcctg caatgtcca actttgaaa ttgacgacg 
      aatttaatc ttcccgtta cgttagcca tttttattc 
      ttgtcgttt atatgtttg tcggcgctc ttatctatt 
      ggctgtggg aggcttgga acttttttg attcttttt 
      actttgttt ttatttcaa tgtcaacag ttggatttg 
      gagatatgg taccgaacg acgccgctt gtatgatgg 
      tctctattg tgtacctag tttttggcc tcgctctaa 
      tgtcgatgt gtatcaatg tggtacaag caaaactga 
      gtgatactt ttcagcaag cttctacta aaattggtg 
      ccactattg gactttccg tcgccgaag atgacggca 
      gcatcacta cagcggttc ccgacaacg ttgaagtgg 
      ttccggttc acgacaagg ccaaaattt cgactaaag 
      acagctgaa taacatttc caaaataat aattaattt 
      ttgacatgt tctatttat tcatcaact acttgtata 
      cctattttt ttttcaata ataaatttt actattaaa 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY