| General information | |||
| TC034465
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 16175687..16181374 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034465 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05075 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC034465 RA
MEFPSDLNA VLKTPNSIS SRKQVDFSR ARPTLVIPP PQDQWTQYT SASSGRTPQ DYSRKQSIF YEGIDGIRD FTKSGLGLG EKCAYWLYN KLKLWSQKW FTHMFLTVV LIFYTIGGA LIFEVIEGR AESTVELDL KDSRDELLR ELRVTSLQT PVEKSLDEW IGEASRHIQ KRYESRLEN YYSHHKLAV TNGIEKKIW TFWNSVVFC STVYTSIEI YDDSKREEK EVDIARIEA MVTPEQMHS ASVAKKRRN FTKRRASRR DKTPLKELD DKTTTDEDV PEQRPKTPE RRRRKKIVS KDESKSEPE FTEKMESAT IKKSHSFSK QVIDEYKKR ETLSELAQA EVVSFFNKT KKGLTAAAI RGRERFKKL DKEQVRRSQ SAPHAFTQE ELIESFRKA QLLDEFNVQ LQTKIYDVY ESDNISVSK QFRPEVQEF VRQRKKQRT TNCSKIVEI LDPKTSNVI AIKSVIKAD FHIRQLFDI VRLFTIKEL LVYFRAFKS DMKDECRKI RILRNRCMC ELFLIITFC GLGGFLFKF VEGSFEHFY KCGVKRVKR DFIEMLWLK SHNLREEEW KSLARNKLR TFEEELHTA HEAGMKTYS GQRSWSFLN SVVYCLTIV TTIGYGHIY PETRTGKAL TIVYSLIGI PLFLLALTD FGKLFTRCI KFLWSFVRR LYYTGSCRK VRKTAHVKE IVKGAQMMY EIATFRRPS VFAEGEQAD TPSPTTPAM SNFEIDDEF NLPVTLAIF ILVVYMFVG ALIYWLWEA WNFFDSFYF VFISMSTVG FGDMVPNDA ACMMVSIVY LVFGLALMS MCINVVQAK LSDTFQQAS TKIGATIGL SVAEDDGSI TTAVPDNVE VVPVHDKAK ISTKDS
- >TC034465 RA
atggaattc ccgagtgat ttaaacgcg gttttaaaa acaccaaat tcaatatca tcgcgtaaa caagtggac tttagtcgt gccagacct actttagtt attcctcca ccacaagat caatggact caatacaca tcagccagt tccggtagg actccacaa gactattca agaaaacaa tcaattttt tacgaagga atcgacgga atcagagat tttacaaag tctggcttg gggcttggg gaaaaatgt gcttattgg ttatacaat aaacttaaa ctttggagt caaaaatgg tttacgcac atgtttttg accgttgtg cttatattt tacactatt ggtggagca ttaattttc gaggtaatt gaaggccga gcggaatca acggtagaa ctagattta aaagattcc agagatgaa cttcttaga gaacttcga gtgacttct ctccaaact cctgttgag aaatctctc gacgaatgg ataggagag gcatcaaga catatccaa aaacgctac gagagtaga ctagaaaat tattattcg catcataag cttgccgtt actaacggt attgaaaag aaaatttgg acgttttgg aattccgtc gttttctgc agtactgtg tacactagt atagaaatt tacgatgat tcaaaacga gaagaaaaa gaagtagat atagctaga atcgaagca atggttaca cctgaacaa atgcatagt gcttctgtg gcaaaaaaa cggcgaaac tttacgaaa cgacgagct agccgtcga gataaaaca cctcttaaa gaactcgac gataaaacg acaacagac gaagatgtt ccagagcaa cgaccaaaa acaccggaa agaagacgt cgaaaaaaa atcgtgagt aaagacgaa tcgaaatct gaaccggag tttactgaa aaaatggaa tctgctact ataaaaaaa tcacacagt ttttccaaa caagtcata gatgagtac aagaaaaga gaaaccttg agtgagtta gcccaggcc gaagttgtt agttttttt aataaaacc aaaaaagga ctaactgca gctgcaata agaggccgc gagagattt aaaaagttg gacaaggaa caggtgaga agaagtcaa tcagcacct cacgcattt acacaagaa gaattgatt gaaagcttt cgcaaagca caacttttg gatgaattt aatgtacaa ctccaaacg aaaatctat gatgtttac gaaagcgac aatatttct gtctcgaaa cagtttcga ccagaggtg caagaattt gtgcgccaa agaaaaaaa caacgcacc acaaattgt tccaaaatt gtagagatt ttagatcca aaaacatcg aatgtgatt gcaattaaa tcagtgata aaagcagat tttcatata aggcaactt tttgatatt gttcgactt tttacgatt aaagaattg ctggtgtat tttcgtgca tttaagtcc gatatgaaa gacgaatgt cgaaagatt cgtatctta agaaatcga tgtatgtgc gaacttttt cttattatt actttttgt ggtctgggt ggattttta tttaaattt gttgaagga agttttgag catttttac aaatgcggc gttaaacga gttaaacgc gactttatc gagatgtta tggcttaaa agtcataat cttagagaa gaagaatgg aaaagttta gcaagaaat aaattgagg acgtttgaa gaagagctc catactgca cacgaagcc ggaatgaaa acatacagt ggtcaaagg tcgtggagt tttctaaat tccgttgtt tattgtcta actatagtt acaaccatt ggttatggt cacatttat cctgaaaca agaactgga aaagctttg accatcgtc tactctctt atcggtatc ccccttttt cttctggca ttaaccgat ttcggcaaa ctctttaca agatgcatt aaatttctt tggtcgttt gttagacga ctttattat actggaagc tgtcgaaaa gtgcgaaaa acagcacac gtgaaggaa attgtcaaa ggagcacaa atgatgtac gaaattgcg acatttcgt cgcccttca gtgttcgcc gagggcgaa caagccgac accccttca ccaacaact cctgcaatg tccaacttt gaaattgac gacgaattt aatcttccc gttacgtta gccattttt attcttgtc gtttatatg tttgtcggc gctcttatc tattggctg tgggaggct tggaacttt tttgattct ttttacttt gtttttatt tcaatgtca acagttgga tttggagat atggtaccg aacgacgcc gcttgtatg atggtctct attgtgtac ctagttttt ggcctcgct ctaatgtcg atgtgtatc aatgtggta caagcaaaa ctgagtgat acttttcag caagcttct actaaaatt ggtgccact attggactt tccgtcgcc gaagatgac ggcagcatc actacagcg gttcccgac aacgttgaa gtggttccg gttcacgac aaggccaaa atttcgact aaagacagc tga
- >TC034465 RA
gcacattgt tgctattta taaacagct ggcagaaaa gatgacgtt gtagaataa gcaaaaatg atttttcat cttggtagg aaactagct cgtaaatat ctaaattta tatcgacaa tatttaaac aacatgaac ccgtatctg ttatgctgc tttgattta atgccctat aatatttta atgcacata aataactcc gtgaataat aaatttaat acagacaaa aattacata aaacaattt ccaggaata tactgttgg gtaaaacta agtacgtta gaaatattc tttctttag taatttgtg aagaattct tgacctaaa ctgccctat ttttaaata attttggct gtgccacta actacctta gtctcaaaa ttctgcgta aaccatcgg atctcatca ctggcgtcg gaaatattt ataagacag tagaaaaca aacggcgtc acgaccagc agagtgcaa ctgtgccgg gatgctatg ttagtttaa cttttgatc gatattgag atgtgatgg aattcccga gtgatttaa acgcggttt taaaaacac caaattcaa tatcatcgc gtaaacaag tggacttta gtcgtgcca gacctactt tagttattc ctccaccac aagatcaat ggactcaat acacatcag ccagttccg gtaggactc cacaagact attcaagaa aacaatcaa ttttttacg aaggaatcg acggaatca gagatttta caaagtctg gcttggggc ttggggaaa aatgtgctt attggttat acaataaac ttaaacttt ggagtcaaa aatggttta cgcacatgt ttttgaccg ttgtgctta tattttaca ctattggtg gagcattaa ttttcgagg taattgaag gccgagcgg aatcaacgg tagaactag atttaaaag attccagag atgaacttc ttagagaac ttcgagtga cttctctcc aaactcctg ttgagaaat ctctcgacg aatggatag gagaggcat caagacata tccaaaaac gctacgaga gtagactag aaaattatt attcgcatc ataagcttg ccgttacta acggtattg aaaagaaaa tttggacgt tttggaatt ccgtcgttt tctgcagta ctgtgtaca ctagtatag aaatttacg atgattcaa aacgagaag aaaaagaag tagatatag ctagaatcg aagcaatgg ttacacctg aacaaatgc atagtgctt ctgtggcaa aaaaacggc gaaacttta cgaaacgac gagctagcc gtcgagata aaacacctc ttaaagaac tcgacgata aaacgacaa cagacgaag atgttccag agcaacgac caaaaacac cggaaagaa gacgtcgaa aaaaaatcg tgagtaaag acgaatcga aatctgaac cggagttta ctgaaaaaa tggaatctg ctactataa aaaaatcac acagttttt ccaaacaag tcatagatg agtacaaga aaagagaaa ccttgagtg agttagccc aggccgaag ttgttagtt tttttaata aaaccaaaa aaggactaa ctgcagctg caataagag gccgcgaga gatttaaaa agttggaca aggaacagg tgagaagaa gtcaatcag cacctcacg catttacac aagaagaat tgattgaaa gctttcgca aagcacaac ttttggatg aatttaatg tacaactcc aaacgaaaa tctatgatg tttacgaaa gcgacaata tttctgtct cgaaacagt ttcgaccag aggtgcaag aatttgtgc gccaaagaa aaaaacaac gcaccacaa attgttcca aaattgtag agattttag atccaaaaa catcgaatg tgattgcaa ttaaatcag tgataaaag cagattttc atataaggc aactttttg atattgttc gacttttta cgattaaag aattgctgg tgtattttc gtgcattta agtccgata tgaaagacg aatgtcgaa agattcgta tcttaagaa atcgatgta tgtgcgaac tttttctta ttattactt tttgtggtc tgggtggat ttttattta aatttgttg aaggaagtt ttgagcatt tttacaaat gcggcgtta aacgagtta aacgcgact ttatcgaga tgttatggc ttaaaagtc ataatctta gagaagaag aatggaaaa gtttagcaa gaaataaat tgaggacgt ttgaagaag agctccata ctgcacacg aagccggaa tgaaaacat acagtggtc aaaggtcgt ggagttttc taaattccg ttgtttatt gtctaacta tagttacaa ccattggtt atggtcaca tttatcctg aaacaagaa ctggaaaag ctttgacca tcgtctact ctcttatcg gtatccccc tttttcttc tggcattaa ccgatttcg gcaaactct ttacaagat gcattaaat ttctttggt cgtttgtta gacgacttt attatactg gaagctgtc gaaaagtgc gaaaaacag cacacgtga aggaaattg tcaaaggag cacaaatga tgtacgaaa ttgcgacat ttcgtcgcc cttcagtgt tcgccgagg gcgaacaag ccgacaccc cttcaccaa caactcctg caatgtcca actttgaaa ttgacgacg aatttaatc ttcccgtta cgttagcca tttttattc ttgtcgttt atatgtttg tcggcgctc ttatctatt ggctgtggg aggcttgga acttttttg attcttttt actttgttt ttatttcaa tgtcaacag ttggatttg gagatatgg taccgaacg acgccgctt gtatgatgg tctctattg tgtacctag tttttggcc tcgctctaa tgtcgatgt gtatcaatg tggtacaag caaaactga gtgatactt ttcagcaag cttctacta aaattggtg ccactattg gactttccg tcgccgaag atgacggca gcatcacta cagcggttc ccgacaacg ttgaagtgg ttccggttc acgacaagg ccaaaattt cgactaaag acagctgaa taacatttc caaaataat aattaattt ttgacatgt tctatttat tcatcaact acttgtata cctattttt ttttcaata ataaatttt actattaaa a
